A dynamic basal complex modulates mammalian sperm movement

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Июнь 21, 2021

Abstract Reproductive success depends on efficient sperm movement driven by axonemal dynein-mediated microtubule sliding. Models predict sliding at the base of tail – centriole but such has never been observed. Centrioles are ancient organelles with a conserved architecture; their rigidity is thought to restrict Here, we show that, in mammalian sperm, atypical distal (DC) and its surrounding pericentriolar matrix form dynamic basal complex (DBC) that facilitates cascade internal deformations, coupling beating asymmetric head kinking. During beating, DC’s right side surroundings slide ~300 nm rostrally relative left side. The deformation throughout DBC transmitted head-tail junction; thus, tilts left, generating kinking motion. These findings suggest evolved as linker into single self-coordinated system.

Язык: Английский

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Energy Metabolism and Hyperactivation of Spermatozoa from Three Mouse Species under Capacitating Conditions

Cells, Год журнала: 2022, Номер 11(2), С. 220 - 220

Опубликована: Янв. 10, 2022

Mammalian sperm differ widely in morphology, and several explanations have been presented to account for this diversity. Less is known about variation physiology cellular processes that can give cells an advantage when competing fertilize oocytes. Capacitation of spermatozoa, a process essential mammalian fertilization, correlates with changes motility result characteristic swimming pattern as hyperactivation. Previous studies revealed velocity depend on the amount ATP available and, therefore, movement occurring during capacitation hyperactivation may involve bioenergetics. Here, we examine differences levels from three mouse species (genus

Язык: Английский

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Evolutionary interactions between thermal ecology and sexual selection

Ecology Letters, Год журнала: 2022, Номер 25(9), С. 1919 - 1936

Опубликована: Июль 13, 2022

Abstract Thermal ecology and mate competition are both pervasive features of ecological adaptation. A surge recent work has uncovered the diversity ways in which temperature affects mating interactions sexual selection. However, potential for thermal biology reproductive to evolve together as organisms adapt their environment been underappreciated. Here, we develop a series hypotheses regarding (1) not only how system dynamics, but also dynamics can generate selection on traits; (2) consequences favour reciprocal co‐adaptation traits. We discuss our context pre‐copulatory post‐copulatory processes. call future integrating experimental phylogenetic comparative approaches understand evolutionary feedbacks between Overall, studying may be necessary have adapted environments past could persist future.

Язык: Английский

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Switches, stability and reversals in the evolutionary history of sexual systems in fish

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Май 30, 2022

Abstract Sexual systems are highly diverse and have profound consequences for population dynamics resilience. Yet, little is known about how they evolved. Using phylogenetic Bayesian modelling a sample of 4614 species, we show that gonochorism the likely ancestral condition in teleost fish. While all hermaphroditic forms revert quickly to gonochorism, protogyny simultaneous hermaphroditism evolutionarily more stable than protandry. In line with theoretical expectations, does not evolve directly from but can slowly sequential hermaphroditism, particularly We find support predictions life history theory protogynous, protandrous, species live longer gonochoristic invest least male gonad mass. The distribution teleosts’ sexual on tree seem reflect just adaptive predictions, suggesting adaptations alone may fully explain why some taxa others (Williams’ paradox). propose future studies should incorporate mating systems, spawning behaviours, diversity sex determining mechanisms. Some latter might constrain evolution while non-duality embryological origin gonads predominates over protandry teleosts.

Язык: Английский

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Seminal fluid gene expression and reproductive fitness in Drosophila melanogaster

BMC Ecology and Evolution, Год журнала: 2022, Номер 22(1)

Опубликована: Фев. 23, 2022

Abstract Background The rapid evolution of seminal fluid proteins (SFPs) has been suggested to be driven by adaptations postcopulatory sexual selection (e.g. sperm competition). However, we have recently shown that most SFPs evolve rapidly under relaxed selective pressures. Given the role in competition for fertilization phenotypes, like ability transfer and store modulation female receptivity ovulation, prevalence selectively appears as a conundrum. One possible explanation is on might terms protein amino acid content, but adjustments expression are essential post-mating function. Interestingly, there general lack systematic implementation gene perturbation assays monitor their effect phenotypes related competition. Results We successfully manipulated 16 SFP encoding genes using tissue-specific knockdowns (KDs) determined these genes’ three important phenotypes: refractoriness remating, defensive (P1), offensive (P2) competitive abilities Drosophila melanogaster . Our analyses show KDs tested do not affect remating P2, however, significantly decreased P1. Moreover, constrained protein-coding sequence lower P1 than evolving selection. Conclusions results suggest more predominant role, previously acknowledged, variation coding changes D.

Язык: Английский

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Spermbots and Their Applications in Assisted Reproduction: Current Progress and Future Perspectives

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 5095 - 5108

Опубликована: Май 1, 2024

Abstract: Sperm quality is declining dramatically during the past decades. Male infertility has been a serious health and social problem. The sperm cell driven biohybrid nanorobot opens new era for automated precise assisted reproduction. Therefore, it urgent necessary to conduct an updated review perspective from viewpoints of researchers clinicians in field reproductive medicine. In present review, we first update current classification, design, control applications various spermbots. Then, by comprehensive summary functional features cells, journey sperms oocyte, sperm-related dysfunctions, provide systematic guidance further improve design Focusing on translation spermbots into clinical practice, point out that main challenges are biocompatibility, effectiveness, ethical issues. Considering special requirements reproduction, also propose three laws usage spermbots: good genetics, gentle operation no contamination. Finally, three-step roadmap proposed achieve goal translation. We believe spermbot-based treatments can be validated approved vitro near future. However, multi-center multi-disciplinary collaborations needed promote vivo applications. Keywords: nanorobot, translation, male infertility, spermbot

Язык: Английский

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An ultra-conserved poison exon in the Tra2b gene encoding a splicing activator is essential for male fertility and meiotic cell division

The EMBO Journal, Год журнала: 2025, Номер 44(3), С. 877 - 902

Опубликована: Янв. 2, 2025

The cellular concentrations of splicing factors (SFs) are critical for controlling alternative splicing. Most serine and arginine-enriched (SR) protein SFs regulate their own concentration via a homeostatic feedback mechanism that involves regulation inclusion non-coding 'poison exons' (PEs) target transcripts nonsense-mediated decay. importance SR PE during animal development is largely unknown despite ultra-conservation across genomes. To address this, we used mouse genetics to disrupt an ultra-conserved in the Tra2b gene encoding Tra2β. Focussing on germ cell development, found deletion causes azoospermia due catastrophic death meiotic prophase. Failure proceed through meiosis was associated with increased expression sufficient drive aberrant Tra2β hyper-responsive splice patterns. Although prophase, spared earlier mitotically active cells, even though these still required function. Our data indicate control prevents accumulation toxic levels incompatible This unexpected connection male fertility helps explain indicates evaluating function models.

Язык: Английский

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What matters to a mouse? Effects of internal and external context on male vocal response to female squeaks

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0312789 - e0312789

Опубликована: Фев. 19, 2025

House mice adjust their signaling behavior depending on the social context of an interaction, but which aspects elicit strongest responses from these individuals is often difficult to determine. To explore how internal and external contextual factors influence house produce respond signals, we assessed dominant subordinate male differed in ultrasonic vocalization (USV) production response playback broadband vocalizations (BBVs, or squeaks) when given limited access a stimulus female. We used repeated measures design each was exposed two types trials with different odor conditions: either just female odors (Fem condition) addition potential competitors, other males (Fem+Male condition). The presence this assay served as proxy for “audience” interacted These conditions were replicated distinct cohorts individuals: familiar competitors Fem+Male condition (Familiar cohort), unfamiliar (Unfamiliar cohort). By assessing dominance status focal individual familiarity “audience”, are able may affect one another BBVs. Dominants subordinates did not differ baseline vocal (vocalizations produced prior squeak playback) squeaks. However, all groups, regardless condition, reduced BBV playback. prompted decrease level calling complexity repertoire compared that only included odor, effect also across status. Importantly, individual. findings suggest alter during courtship interactions cues indicate modulated by competitor cues.

Язык: Английский

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Evolutionary Aspects of Sperm Physiology and Its Assessment

Methods in molecular biology, Год журнала: 2025, Номер unknown, С. 29 - 41

Опубликована: Янв. 1, 2025

Язык: Английский

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Lateralized courtship behavior in Ostrinia furnacalis (Lepidoptera: Crambidae): influence of gender, sexual experience, and its effects on mating success

Environmental Entomology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Abstract Lateralization in mating behavior is increasingly recognized as a significant trait insect species, yet its influence associated with gender, and sexual experience Ostrinia furnacalis (Guenée) remains poorly understood. This study examines how lateralized behaviors, interact to success efficiency O. furnacalis. We conducted controlled trials assess gender shape directional approaches (eg right- or left-biased) turnings 180° across the pre-copulatory, copulatory, post-copulatory phases. Our results indicated that, terms of males were more likely approach females, whereas females rarely initiated approaches, both each other simultaneously being infrequent. Both virgin experienced showed higher right-biased than front left-biased turns for successful intromissive copulation. Experienced greater virgins. In contrast, exhibited lower longer interactions compared particularly duration Post-copulatory that antennal touching occurred frequently pairs. first demonstrate combined on dynamics, male courtship behaviors linked learning processes. The indicate experience, potentially involving memory processes, significantly enhances fitness research provides nuanced understanding furnacalis, implications refining pest management strategies agricultural environments.

Язык: Английский

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