Genomic epidemiology of the clinically dominant clonal complex 1 in the Listeria monocytogenes population in the UK DOI Creative Commons
Emily T. Fotopoulou, Claire Jenkins, Clare R. Barker

и другие.

Microbial Genomics, Год журнала: 2024, Номер 10(1)

Опубликована: Янв. 2, 2024

Listeria monocytogenes is a food-borne pathogen, typically affecting the elderly, immunocompromised patients and pregnant women. The aim of this study was to determine population structure L. clonal complex 1 (CC1) in UK describe genomic epidemiology clinically significant CC. We interrogated working dataset 4073 sequences isolated between January 2015 December 2020 from human clinical specimens, food and/or food-production environments. A minimum spanning tree reconstructed UK. Subsequent analysis focused on CC1, as cause highest proportion invasive listeriosis humans. Sequencing data integrated with metadata environmental isolates, information patient questionnaires, including age, sex outcomes. All isolates either belonged lineage I ( n =1299/4073, 32%) or II =2774/4073, 68%), cases more likely belong =546/928, 59%) =2352/3067, 77%). Of four largest CCs, CC1 =237) had disease (CC1 =160/237, 67.5 %; CC121 =13/843, 2 CC9 =53/360, 15 CC2 =69/339, 20%). Within most were female =95/160, 59%, P =0.01771) people >60 years old (39/95, 41%, =1.314×10 −6 ) high number them aged 20–39 =35/95, 37%) linked pregnancy-related =29/35, 83%). Most male men over 60 (40/65, 62%), fatal both males females identified age group (42/55, 76%). Phylogenetic revealed 23 5 SNP single linkage clusters comprising 80/237 (34 %) cluster sizes ranging 19. Five comprised an implicated item. Expanding 25 resolved additional two linking potential vehicle. Analysis demographic outcome elderly women child-bearing age. small, sparsely populated clusters. Only containing vehicle, processing farming environments, resolved, emphasizing need for clinical, animal-health agencies share sequencing real time, importance One Health approach public-health surveillance listeriosis.

Язык: Английский

Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for Twelve Antimicrobials (Biocides and Antibiotics) in Eight Strains of Listeria monocytogenes DOI Creative Commons
Cristina Rodríguez‐Melcón, Carlos Alonso‐Calleja, María del Camino García Fernández

и другие.

Biology, Год журнала: 2021, Номер 11(1), С. 46 - 46

Опубликована: Дек. 29, 2021

When selecting effective doses of antimicrobials, be they biocides or antibiotics, it is essential to know the minimum inhibitory concentrations (MICs) and bactericidal (MBCs) these substances. The present research determined MICs MBCs for three biocides, sodium hypochlorite (SH), benzalkonium chloride (BC), peracetic acid (PAA), nine antibiotics in eight strains Listeria monocytogenes varying serotypes. Marked intra-species differences were observed resistance L. antibiotics. (ppm) ranged between 1750 4500 SH, 0.25 20.00 BC, 1050 1700 PAA. Their from 2250 0.50 1150 1800 lay 1 15 ampicillin, 8 150 cephalothin, 20 170 cefoxitin, 0.05 0.20 erythromycin, 4 50 chloramphenicol, 3 100 gentamicin, 2 tetracycline, 80 vancomycin, 160 430 fosfomycin. corresponding 5 9 70 200 30 90 450 Notably, erythromycin showed considerable efficacy, demonstrated by low values both MIC MBC. Based on EUCAST CLSI criteria, all susceptible erythromycin. All resistant Further 87.50% ampicillin 75.00% 62.50% chloramphenicol. high prevalence antibiotic a matter concern. A positive correlation was found MBC most higher hydrophobicity cell surface, susceptibility suggesting that surface characteristics bacterial cells influence compounds.

Язык: Английский

Процитировано

133
Nationwide genomic atlas of soil-dwelling Listeria reveals effects of selection and population ecology on pangenome evolution DOI
Jingqiu Liao, Xiaodong Guo, Daniel L. Weller

и другие.

Nature Microbiology, Год журнала: 2021, Номер 6(8), С. 1021 - 1030

Опубликована: Июль 15, 2021

Язык: Английский

Процитировано

76
Listeria monocytogenes—How This Pathogen Uses Its Virulence Mechanisms to Infect the Hosts DOI Creative Commons
Jacek Osek, Kinga Wieczorek

Pathogens, Год журнала: 2022, Номер 11(12), С. 1491 - 1491

Опубликована: Дек. 7, 2022

Listeriosis is a serious food-borne illness, especially in susceptible populations, including children, pregnant women, and elderlies. The disease can occur two forms: non-invasive febrile gastroenteritis severe invasive listeriosis with septicemia, meningoencephalitis, perinatal infections, abortion. Expression of each symptom depends on various bacterial virulence factors, immunological status the infected person, number ingested bacteria. Internalins, mainly InlA InlB, invasins (invasin A, LAP), other surface adhesion proteins (InlP1, InlP4) are responsible for epithelial cell binding, whereas internalin C (InlC) actin assembly-inducing protein (ActA) involved cell-to-cell spread. L. monocytogenes able to disseminate through blood invade diverse host organs. In persons impaired immunity, elderly, pathogen also cross blood-brain placental barriers, which results invasion central nervous system fetus infection, respectively. aim this comprehensive review summarize current knowledge epidemiology mechanisms that special focus their molecular cellular aspects. We believe all information crucial better understanding pathogenesis infection.

Язык: Английский

Процитировано

49
Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens DOI Creative Commons

Rebecca Bland,

Stephanie R.B. Brown, Joy Waite‐Cusic

и другие.

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2022, Номер 21(2), С. 1777 - 1802

Опубликована: Фев. 24, 2022

Abstract The development of antibiotic resistance is a serious public health crisis, reducing our ability to effectively combat infectious bacterial diseases. parallel study reduced susceptibility sanitizers growing, particularly for environmental foodborne pathogens, such as Listeria monocytogenes . As regulations demand seek‐and‐destroy approach L. , understanding sanitizer efficacy and its uses are critical the food industry. Studies have reported survive in concentrations 10–1000 times lower than manufacturer‐recommended concentration (MRC). Notably, data show that at MRC when applied according label instructions, remain largely effective. also report variables presence organic material, application time/temperature, attachment surfaces can impact effectiveness. Due lack standardization methodology definitions resistance, tolerance, susceptibility, different messages conveyed studies. In this review, we examine diversity definitions, terminology, methodologies used studies examining antimicrobials. Research available date fails demonstrate “resistance” recommended treatments prescribed by label. such, tolerance would be more accurate description response low (i.e., sub‐MRC). Conservative use word will reduce confusion allow concise messaging research findings communicated industry regulators.

Язык: Английский

Процитировано

47
Landscape of Stress Response and Virulence Genes Among Listeria monocytogenes Strains DOI Creative Commons
Brankica Lakićević, Heidy M.W. den Besten, Daniela De Biase

и другие.

Frontiers in Microbiology, Год журнала: 2022, Номер 12

Опубликована: Янв. 20, 2022

The pathogenic microorganism Listeria monocytogenes is ubiquitous and responsible for listeriosis, a disease with high mortality rate in susceptible people. It can persist different habitats, including the farm environment, food production environments, foods. This pathogen grow under challenging conditions, such as low pH, temperatures, salt concentrations. However, L. has degree of strain divergence regarding virulence potential, environmental adaption, stress response. review seeks to provide reader an up-to-date overview clonal serotype-specific differences among strains. Emphasis on genes genomic islands resistance stresses given explain complex adaptation Moreover, we highlight use advanced diagnostic technologies, whole-genome sequencing, fine-tune quantitative microbiological risk assessment better control listeriosis.

Язык: Английский

Процитировано

39
Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges DOI Creative Commons
Joshua Hadi, Delphine Rapp, Sharduli Dhawan

и другие.

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2023, Номер 22(3), С. 2433 - 2464

Опубликована: Апрель 11, 2023

The global food demand is expected to increase in the coming years, along with challenges around climate change and security. Concomitantly, safety risks, particularly those related bacterial pathogens, may also increase. Thus, sector needs innovate rise this challenge. Here, we discuss recent advancements molecular techniques that can be deployed within various foodborne bacteria surveillance systems across settings. To start with, provide updates on nucleic acid-based detection, a focus polymerase chain reaction (PCR)-based technologies loop-mediated isothermal amplification (LAMP). These include descriptions of novel genetic markers for several progresses multiplex PCR droplet digital PCR. next section provides an overview development clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) proteins systems, such as CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a, tools enhanced sensitive specific detection pathogens. final describes utilizations whole genome sequencing accurate characterization bacteria, ranging from epidemiological model-based predictions phenotypic traits through genome-wide association studies or machine learning.

Язык: Английский

Процитировано

21
The Saprophytic Lifestyle of Listeria monocytogenes and Entry Into the Food-Processing Environment DOI Creative Commons
António Lourenço,

Kristina Linke,

Martin Wagner

и другие.

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Март 8, 2022

Listeria monocytogenes is an environmentally adapted saprophyte that can change into a human and animal bacterial pathogen with zoonotic potential through several regulatory systems. In this review, the focus on occurrence of sensu stricto lato in different ecological niches, detection methods, their analytical limitations. It also highlights L. genotypes environment (soil, water, wildlife), reflects molecular determinants for saprophytic lifestyle antibiotic resistance. particular, strain-specific properties which some circulate wastewater, surface soil, wildlife, agricultural environments are particular interest continuously updating risk analysis.

Язык: Английский

Процитировано

24
Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022 DOI Creative Commons
Renato H. Orsi, Jingqiu Liao, Catharine R. Carlin

и другие.

mBio, Год журнала: 2023, Номер 15(2)

Опубликована: Дек. 21, 2023

Since 2010, the genus Listeria has had addition of 22 new species that more than tripled number identified until 2010. Sixteen these are distantly related to type species, monocytogenes, and several present phenotypes distinguish them from classical (L. innocua, ivanovii, seeligeri, welshimeri, grayi). These newly described also show is genetically diverse previously estimated. While future studies surveys needed clarify distribution at least some may not be widely spread, while other frequently found spread human-related settings (e.g., farms processing facilities), others adapted specific environmental habitats. Here, we review taxonomic, phylogenetic, ecological characteristics since 2010 re-iterate suggestion re-classification into three genera: Murraya, Mesolisteria, Paenilisteria. We provide a current detection issues relevance food safety identification species. For example, non-pathogenic could misidentified as pathogen L. based on methods do target monocytogenes-specific virulence genes/factors, leading unnecessary product recalls. Moreover, eight in proposed Mesolisteria good indicators conditions allow monocytogenes grow unable low temperatures.

Язык: Английский

Процитировано

14
Ruminant‐associated Listeria monocytogenes isolates belong preferentially to dairy‐associated hypervirulent clones: a longitudinal study in 19 farms DOI
Carla Palacios‐Gorba, Alexandra Moura, Jesús Gomis

и другие.

Environmental Microbiology, Год журнала: 2021, Номер 23(12), С. 7617 - 7631

Опубликована: Дек. 1, 2021

Summary Studies have shown that ruminants constitute reservoirs of Listeria monocytogenes , but little is known about the epidemiology and genetic diversity this pathogen within farms. Here we conducted a large‐scale longitudinal study to monitor spp. in 19 dairy farms during three consecutive seasons ( N = 3251 samples). L . innocua was most prevalent species, followed by detected 52.6% more frequently cattle (4.1%) sheep (4.5%) than goat (0.2%). Lineage I accounted for 69% isolates. Among animal samples, sublineages (SL) clonal complexes (CC) were SL1/CC1, SL219/CC4, SL26/CC26 SL87/CC87, whereas SL666/CC666 environmental samples. Sixty‐one different cgMLST types found, 28% common animals and/or surfaces same farm 21% previously reported elsewhere context food human surveillance. prevalence not affected hygiene season: higher observed winter cattle, spring Cows their second lactation had probability faecal shedding. This highlights as reservoir hypervirulent

Язык: Английский

Процитировано

30
Prevalence, identification and antimicrobial resistance of Listeria monocytogenes and Listeria spp. isolated from poultry and pork meat DOI Creative Commons

Alba Martínez‐Laorden,

Celia Arraiz-Fernández,

María J. Cantalejo

и другие.

International Journal of Food Science & Technology, Год журнала: 2024, Номер 59(4), С. 2667 - 2675

Опубликована: Фев. 25, 2024

Summary The aim of this work was to evaluate the prevalence Listeria monocytogenes and other spp. in chicken, duck, quail, turkey pork meat, including antibiotic resistance isolated strains. A total 184 meat samples were collected from different retailers La Rioja (Spain). presence L . detected 24.46% 10.32% respectively. predominant found quail while innocua welshimeri species duck thirty‐three strains (55.93%) be multi‐resistant (resistant ≥3 families antibiotics). highest rates observed (73.68%) (70.59%), followed by ivanovii (50%). Resistance ampicillin trimethoprim–sulfamethoxazole being special concern, since these antibiotics are used treatment listeriosis. Special measures should taken reduce contamination such as adequate handling, correct preparation (cooking) cleaning disinfection order avoid cross‐contamination.

Язык: Английский

Процитировано

4