Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for Twelve Antimicrobials (Biocides and Antibiotics) in Eight Strains of Listeria monocytogenes

Biology, Journal Year: 2021, Volume and Issue: 11(1), P. 46 - 46

Published: Dec. 29, 2021

When selecting effective doses of antimicrobials, be they biocides or antibiotics, it is essential to know the minimum inhibitory concentrations (MICs) and bactericidal (MBCs) these substances. The present research determined MICs MBCs for three biocides, sodium hypochlorite (SH), benzalkonium chloride (BC), peracetic acid (PAA), nine antibiotics in eight strains Listeria monocytogenes varying serotypes. Marked intra-species differences were observed resistance L. antibiotics. (ppm) ranged between 1750 4500 SH, 0.25 20.00 BC, 1050 1700 PAA. Their from 2250 0.50 1150 1800 lay 1 15 ampicillin, 8 150 cephalothin, 20 170 cefoxitin, 0.05 0.20 erythromycin, 4 50 chloramphenicol, 3 100 gentamicin, 2 tetracycline, 80 vancomycin, 160 430 fosfomycin. corresponding 5 9 70 200 30 90 450 Notably, erythromycin showed considerable efficacy, demonstrated by low values both MIC MBC. Based on EUCAST CLSI criteria, all susceptible erythromycin. All resistant Further 87.50% ampicillin 75.00% 62.50% chloramphenicol. high prevalence antibiotic a matter concern. A positive correlation was found MBC most higher hydrophobicity cell surface, susceptibility suggesting that surface characteristics bacterial cells influence compounds.

Language: Английский

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Nationwide genomic atlas of soil-dwelling Listeria reveals effects of selection and population ecology on pangenome evolution

Nature Microbiology, Journal Year: 2021, Volume and Issue: 6(8), P. 1021 - 1030

Published: July 15, 2021

Language: Английский

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Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2022, Volume and Issue: 21(2), P. 1777 - 1802

Published: Feb. 24, 2022

Abstract The development of antibiotic resistance is a serious public health crisis, reducing our ability to effectively combat infectious bacterial diseases. parallel study reduced susceptibility sanitizers growing, particularly for environmental foodborne pathogens, such as Listeria monocytogenes . As regulations demand seek‐and‐destroy approach L. , understanding sanitizer efficacy and its uses are critical the food industry. Studies have reported survive in concentrations 10–1000 times lower than manufacturer‐recommended concentration (MRC). Notably, data show that at MRC when applied according label instructions, remain largely effective. also report variables presence organic material, application time/temperature, attachment surfaces can impact effectiveness. Due lack standardization methodology definitions resistance, tolerance, susceptibility, different messages conveyed studies. In this review, we examine diversity definitions, terminology, methodologies used studies examining antimicrobials. Research available date fails demonstrate “resistance” recommended treatments prescribed by label. such, tolerance would be more accurate description response low (i.e., sub‐MRC). Conservative use word will reduce confusion allow concise messaging research findings communicated industry regulators.

Language: Английский

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Listeria monocytogenes—How This Pathogen Uses Its Virulence Mechanisms to Infect the Hosts

Pathogens, Journal Year: 2022, Volume and Issue: 11(12), P. 1491 - 1491

Published: Dec. 7, 2022

Listeriosis is a serious food-borne illness, especially in susceptible populations, including children, pregnant women, and elderlies. The disease can occur two forms: non-invasive febrile gastroenteritis severe invasive listeriosis with septicemia, meningoencephalitis, perinatal infections, abortion. Expression of each symptom depends on various bacterial virulence factors, immunological status the infected person, number ingested bacteria. Internalins, mainly InlA InlB, invasins (invasin A, LAP), other surface adhesion proteins (InlP1, InlP4) are responsible for epithelial cell binding, whereas internalin C (InlC) actin assembly-inducing protein (ActA) involved cell-to-cell spread. L. monocytogenes able to disseminate through blood invade diverse host organs. In persons impaired immunity, elderly, pathogen also cross blood-brain placental barriers, which results invasion central nervous system fetus infection, respectively. aim this comprehensive review summarize current knowledge epidemiology mechanisms that special focus their molecular cellular aspects. We believe all information crucial better understanding pathogenesis infection.

Language: Английский

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Landscape of Stress Response and Virulence Genes Among Listeria monocytogenes Strains

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: Jan. 20, 2022

The pathogenic microorganism Listeria monocytogenes is ubiquitous and responsible for listeriosis, a disease with high mortality rate in susceptible people. It can persist different habitats, including the farm environment, food production environments, foods. This pathogen grow under challenging conditions, such as low pH, temperatures, salt concentrations. However, L. has degree of strain divergence regarding virulence potential, environmental adaption, stress response. review seeks to provide reader an up-to-date overview clonal serotype-specific differences among strains. Emphasis on genes genomic islands resistance stresses given explain complex adaptation Moreover, we highlight use advanced diagnostic technologies, whole-genome sequencing, fine-tune quantitative microbiological risk assessment better control listeriosis.

Language: Английский

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Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2023, Volume and Issue: 22(3), P. 2433 - 2464

Published: April 11, 2023

The global food demand is expected to increase in the coming years, along with challenges around climate change and security. Concomitantly, safety risks, particularly those related bacterial pathogens, may also increase. Thus, sector needs innovate rise this challenge. Here, we discuss recent advancements molecular techniques that can be deployed within various foodborne bacteria surveillance systems across settings. To start with, provide updates on nucleic acid-based detection, a focus polymerase chain reaction (PCR)-based technologies loop-mediated isothermal amplification (LAMP). These include descriptions of novel genetic markers for several progresses multiplex PCR droplet digital PCR. next section provides an overview development clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) proteins systems, such as CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a, tools enhanced sensitive specific detection pathogens. final describes utilizations whole genome sequencing accurate characterization bacteria, ranging from epidemiological model-based predictions phenotypic traits through genome-wide association studies or machine learning.

Language: Английский

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The Saprophytic Lifestyle of Listeria monocytogenes and Entry Into the Food-Processing Environment

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: March 8, 2022

Listeria monocytogenes is an environmentally adapted saprophyte that can change into a human and animal bacterial pathogen with zoonotic potential through several regulatory systems. In this review, the focus on occurrence of sensu stricto lato in different ecological niches, detection methods, their analytical limitations. It also highlights L. genotypes environment (soil, water, wildlife), reflects molecular determinants for saprophytic lifestyle antibiotic resistance. particular, strain-specific properties which some circulate wastewater, surface soil, wildlife, agricultural environments are particular interest continuously updating risk analysis.

Language: Английский

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Ruminant‐associated Listeria monocytogenes isolates belong preferentially to dairy‐associated hypervirulent clones: a longitudinal study in 19 farms

Environmental Microbiology, Journal Year: 2021, Volume and Issue: 23(12), P. 7617 - 7631

Published: Dec. 1, 2021

Summary Studies have shown that ruminants constitute reservoirs of Listeria monocytogenes , but little is known about the epidemiology and genetic diversity this pathogen within farms. Here we conducted a large‐scale longitudinal study to monitor spp. in 19 dairy farms during three consecutive seasons ( N = 3251 samples). L . innocua was most prevalent species, followed by detected 52.6% more frequently cattle (4.1%) sheep (4.5%) than goat (0.2%). Lineage I accounted for 69% isolates. Among animal samples, sublineages (SL) clonal complexes (CC) were SL1/CC1, SL219/CC4, SL26/CC26 SL87/CC87, whereas SL666/CC666 environmental samples. Sixty‐one different cgMLST types found, 28% common animals and/or surfaces same farm 21% previously reported elsewhere context food human surveillance. prevalence not affected hygiene season: higher observed winter cattle, spring Cows their second lactation had probability faecal shedding. This highlights as reservoir hypervirulent

Language: Английский

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Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022

mBio, Journal Year: 2023, Volume and Issue: 15(2)

Published: Dec. 21, 2023

Since 2010, the genus Listeria has had addition of 22 new species that more than tripled number identified until 2010. Sixteen these are distantly related to type species, monocytogenes, and several present phenotypes distinguish them from classical (L. innocua, ivanovii, seeligeri, welshimeri, grayi). These newly described also show is genetically diverse previously estimated. While future studies surveys needed clarify distribution at least some may not be widely spread, while other frequently found spread human-related settings (e.g., farms processing facilities), others adapted specific environmental habitats. Here, we review taxonomic, phylogenetic, ecological characteristics since 2010 re-iterate suggestion re-classification into three genera: Murraya, Mesolisteria, Paenilisteria. We provide a current detection issues relevance food safety identification species. For example, non-pathogenic could misidentified as pathogen L. based on methods do target monocytogenes-specific virulence genes/factors, leading unnecessary product recalls. Moreover, eight in proposed Mesolisteria good indicators conditions allow monocytogenes grow unable low temperatures.

Language: Английский

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Prevalence, identification and antimicrobial resistance of Listeria monocytogenes and Listeria spp. isolated from poultry and pork meat

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: 59(4), P. 2667 - 2675

Published: Feb. 25, 2024

Summary The aim of this work was to evaluate the prevalence Listeria monocytogenes and other spp. in chicken, duck, quail, turkey pork meat, including antibiotic resistance isolated strains. A total 184 meat samples were collected from different retailers La Rioja (Spain). presence L . detected 24.46% 10.32% respectively. predominant found quail while innocua welshimeri species duck thirty‐three strains (55.93%) be multi‐resistant (resistant ≥3 families antibiotics). highest rates observed (73.68%) (70.59%), followed by ivanovii (50%). Resistance ampicillin trimethoprim–sulfamethoxazole being special concern, since these antibiotics are used treatment listeriosis. Special measures should taken reduce contamination such as adequate handling, correct preparation (cooking) cleaning disinfection order avoid cross‐contamination.

Language: Английский

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