A New Understanding of Long Non-Coding RNA in Hepatocellular Carcinoma—From m6A Modification to Blood Biomarkers DOI Creative Commons
Jung Woo Eun, Jae Youn Cheong, Jee‐Yeong Jeong

и другие.

Cells, Год журнала: 2023, Номер 12(18), С. 2272 - 2272

Опубликована: Сен. 14, 2023

With recent advancements in biological research, long non-coding RNAs (lncRNAs) with lengths exceeding 200 nucleotides have emerged as pivotal regulators of gene expression and cellular phenotypic modulation. Despite initial skepticism due to their low sequence conservation levels, significance various processes has become increasingly apparent. We provided an overview lncRNAs discussed defining features modes operation. then explored crucial function the hepatocarcinogenesis process, elucidating complex involvement hepatocellular carcinoma (HCC). The influential role within HCC tumor microenvironment is emphasized, illustrating potential key modulators disease dynamics. also investigated significant influence N6-methyladenosine (m6A) modification on lncRNA HCC, enhancing our understanding both roles upstream regulators. Additionally, promising biomarkers was liver cancer diagnosis, suggesting a novel avenue for future research clinical application. Finally, work underscored dual contributors pathogenesis innovative tools its diagnosis. Existing challenges prospective trajectories are discussed, emphasizing advancing research.

Язык: Английский

Involvement of lncRNAs in cancer cells migration, invasion and metastasis: cytoskeleton and ECM crosstalk DOI Creative Commons
Mohammad Ahmad, Louis‐Bastien Weiswald, Laurent Poulain

и другие.

Journal of Experimental & Clinical Cancer Research, Год журнала: 2023, Номер 42(1)

Опубликована: Июль 18, 2023

Cancer is the main cause of death worldwide and metastasis a major poor prognosis cancer-associated mortality. Metastatic conversion cancer cells multiplex process, including EMT through cytoskeleton remodeling interaction with TME. Tens thousands putative lncRNAs have been identified, but biological functions most are still to be identified. However, already emerged as key regulators gene expression at transcriptional post-transcriptional level control in spatio-temporal fashion. LncRNA-dependent mechanisms can cell fates during development their perturbed associated onset progression many diseases cancer. LncRNAs involved each step different modes action. The investigation roles could possibly lead identification new biomarkers innovative therapeutic options.

Язык: Английский

Процитировано

65
LncRNA HOTAIR promotes the growth and metastasis of gastric cancer by sponging miR-1277-5p and upregulating COL5A1 DOI Open Access

Zhijian Wei,

Lei Chen,

Lei Meng

и другие.

Gastric Cancer, Год журнала: 2020, Номер 23(6), С. 1018 - 1032

Опубликована: Июнь 24, 2020

Язык: Английский

Процитировано

79
Upregulation of lncRNA NIFK-AS1 in hepatocellular carcinoma by m6A methylation promotes disease progression and sorafenib resistance DOI
Yitian Chen, Dan Xiang, Xiaoyue Zhao

и другие.

Human Cell, Год журнала: 2021, Номер 34(6), С. 1800 - 1811

Опубликована: Авг. 10, 2021

Язык: Английский

Процитировано

60
Identification of lncRNA/circRNA-miRNA-mRNA ceRNA Network as Biomarkers for Hepatocellular Carcinoma DOI Creative Commons
Shanshan Chen, Yongchao Zhang, Xiaoyan Ding

и другие.

Frontiers in Genetics, Год журнала: 2022, Номер 13

Опубликована: Март 21, 2022

Background: Hepatocellular carcinoma (HCC) accounts for the majority of liver cancer, with incidence and mortality rates increasing every year. Despite improvement clinical management, substantial challenges remain due to its high recurrence short survival period. This study aimed identify potential diagnostic prognostic biomarkers in HCC through bioinformatic analysis. Methods: Datasets from GEO TCGA databases were used Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses carried out by WebGestalt website clusterProfiler package R. The STRING database Cytoscape software establish protein-protein interaction (PPI) network. GEPIA was perform expression genes. miRDB, miRWalk, TargetScan employed predict miRNAs levels predicted explored via OncomiR database. LncRNAs StarBase LncBase while circRNA prediction performed circBank. ROC curve analysis Kaplan-Meier (KM) evaluate value gene expression, respectively. Results: A total 327 upregulated 422 downregulated overlapping DEGs identified between tissues noncancerous tissues. PPI network constructed 89 nodes 178 edges eight hub genes selected upstream ceRNAs. lncRNA/circRNA-miRNA-mRNA successfully based on ceRNA hypothesis, including five lncRNAs (DLGAP1-AS1, GAS5, LINC00665, TYMSOS, ZFAS1), six circRNAs (hsa_circ_0003209, hsa_circ_0008128, hsa_circ_0020396, hsa_circ_0030051, hsa_circ_0034049, hsa_circ_0082333), (hsa-miR-150-5p, hsa-miR-19b-3p, hsa-miR-23b-3p, hsa-miR-26a-5p, hsa-miR-651-5p, hsa-miR-10a-5p, hsa-miR-214-5p hsa-miR-486-5p), mRNAs (CDC6, GINS1, MCM4, MCM6, MCM7). can promote progression cell cycle, DNA replication, other pathways. Clinical demonstrated that ZFAS1/hsa-miR-150-5p/GINS1 regulatory axis had a value. Conclusion: These results revealed cycle replication pathway could be pathways participate development. is expected provide therapeutic targets especially axis.

Язык: Английский

Процитировано

57
Long Non-coding RNA DANCR as an Emerging Therapeutic Target in Human Cancers DOI Creative Commons

Shi-Jia Jin,

Mingzhu Jin, Bairong Xia

и другие.

Frontiers in Oncology, Год журнала: 2019, Номер 9

Опубликована: Ноя. 15, 2019

Long noncoding RNAs (lncRNAs) are emerging as important regulators of numerous biological processes, especially in cancer development. Aberrantly expressed and specifically located tumor cells, they exert distinct functions different cancers via regulating multiple downstream targets such chromatins, RNAs, proteins. Differentiation antagonizing non-protein coding RNA (DANCR) is a cytoplasmic lncRNA that generally works promoter. Mechanically, DANCR promotes the vital components oncogene network by sponging their corresponding microRNAs or interacting with various DANCR's expression cells collective involvement pro-tumor pathways make it promising therapeutic target for broad treatment. Herein, we summarize molecular mechanism human cancers. Furthermore, introduce use CRISPR/Cas9, antisense oligonucleotides small interfering well viral, lipid, exosomal vectors onco-lncRNA targeted Conclusively, considerable promoter bright prospect therapy.

Язык: Английский

Процитировано

66
miR-335-5p inhibits TGF-β1-induced epithelial–mesenchymal transition in non-small cell lung cancer via ROCK1 DOI Creative Commons
Wenwen Du, Haicheng Tang, Zhe Lei

и другие.

Respiratory Research, Год журнала: 2019, Номер 20(1)

Опубликована: Окт. 21, 2019

Significant evidence has shown that the miRNA pathway is an important component in downstream signaling cascades of TGF-β1 pathway. Our previous study indicated miR-335-5p expression was significantly down-regulated and acted as a vital player metastasis non-small cell lung cancer (NSCLC), however underlying mechanism remained unclear.The differential level ROCK1 were determined by qRT-PCR IHC analysis human tissue samples with or without lymph node metastasis. Transwell assay conducted to determine ability migration invasion. SiRNA interference, microRNA transfection western blot utilized clarify regulatory mechanism.We showed up-regulated NSCLC tissues associated Over-expresion inhibited TGF-β1-mediated Furthermore, luciferase reporter assays proved can bind 3'-UTR directly. Moreover, we confirmed siRNA-mediated silencing diminished EMT migratory invasive capabilities A549 SPC-A1 cells.This first time report regulates impairs its functions, thereby playing key role TGF-β1-induced invasion NSCLC.

Язык: Английский

Процитировано

64
LncRNA ANCR promotes hepatocellular carcinoma metastasis through upregulating HNRNPA1 expression DOI Open Access
Zhili Wen,

Lingyan Lian,

Hao Ding

и другие.

RNA Biology, Год журнала: 2019, Номер 17(3), С. 381 - 394

Опубликована: Дек. 23, 2019

LncRNA ANCR plays important roles in the modulation of epithelial mesenchymal transition (EMT) and tumour metastasis many tumours. However, role regulating hepatocellular carcinoma (HCC) is still not known. The current study aims to investigate underlying mechanism for oncogenesis HCC metastasis. cell proliferation migration/invasion were measured by MTT Transwell assays. Xenograft model was established determine effect on growth ChIP assay used detect H3 H4 histone acetylation levels at promoter region. RNA pull-down RIP performed analyse relationship between heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1). Dual-luciferase reporter gene conducted interaction miR-140-3p. results indicated that highly expressed tissues cells, which promoted cells. In vivo experiments showed interfering suppressed HCC. H3/H4 region elevated deacetylases 3 (HDAC3) significantly up-regulated expression. could bind HNRNPA1, expression HNRNPA1 through its degradation. addition, upregulated sponging Finally, we found EMT invasion/migration cells HNRNPA1. conclusion, upregulating inhibiting degradation

Язык: Английский

Процитировано

57
Long noncoding RNA LINC00941 promotes pancreatic cancer progression by competitively binding miR-335-5p to regulate ROCK1-mediated LIMK1/Cofilin-1 signaling DOI Creative Commons
Jie Wang, Zhiwei He, Jian Xu

и другие.

Cell Death and Disease, Год журнала: 2021, Номер 12(1)

Опубликована: Янв. 4, 2021

Abstract An accumulation of evidence indicates that long noncoding RNAs are involved in the tumorigenesis and progression pancreatic cancer (PC). In this study, we investigated functions molecular mechanism action LINC00941 PC. Quantitative PCR was used to examine expression miR-335-5p PC tissues cell lines, investigate correlation between clinicopathological features. Plasmid vectors or lentiviruses were manipulate LINC00941, miR-335-5p, ROCK1 lines. Gain loss-of-function assays mechanistic employed verify roles growth metastasis, both vivo vitro. found be highly expressed PC, while exhibited low expression. High strongly associated with larger tumor size, lymph node poor prognosis. Functional experiments revealed silencing significantly suppressed growth, metastasis epithelial–mesenchymal transition. functioned as a sponge for competitive endogenous RNA (ceRNA) ROCK1, promoting upregulation, LIMK1/Cofilin-1 pathway activation. Our observations lead us conclude an oncogene progression, behaving ceRNA binding. may therefore have potential utility diagnostic treatment target disease.

Язык: Английский

Процитировано

53
Exosomes secreted by M2 macrophages promote cancer stemness of hepatocellular carcinoma via the miR-27a-3p/TXNIP pathways DOI
Wenxiao Li,

Xiaowen Xin,

Xiujun Li

и другие.

International Immunopharmacology, Год журнала: 2021, Номер 101, С. 107585 - 107585

Опубликована: Сен. 30, 2021

Язык: Английский

Процитировано

51
Long Non-coding RNA DANCR in Cancer: Roles, Mechanisms, and Implications DOI Creative Commons

Maoye Wang,

Jianmei Gu, Xu Zhang

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Окт. 15, 2021

Long non-coding RNA (lncRNA) DANCR (also known as ANCR)—differentiation antagonizing non-protein coding RNA, was first reported in 2012 to suppress differentiation of epithelial cells. Emerging evidence demonstrates that is a cancer-associated lncRNA abnormally expressed many cancers (e.g., lung cancer, gastric breast hepatocellular carcinoma). Increasing studies suggest the dysregulation plays critical roles cancer cell proliferation, apoptosis, migration, invasion, and chemoresistance vitro tumor growth metastasis vivo . Mechanistic analyses show can serve miRNA sponges, stabilize mRNAs, interact with proteins. Recent research reveals be detected body fluids such serum, plasma, exosomes, providing quick convenient method for monitor. Thus used promising diagnostic prognostic biomarker therapeutic target various types cancer. This review focuses on role mechanism progression an emphasis clinical significance human cancers.

Язык: Английский

Процитировано

43