A lasting symbiosis: how the Hawaiian bobtail squid finds and keeps its bioluminescent bacterial partner

Nature Reviews Microbiology, Год журнала: 2021, Номер 19(10), С. 666 - 679

Опубликована: Июнь 4, 2021

Язык: Английский

---

A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 21, 2023

Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level stabilizing their colonization. Primary colonizers saturate exclude secondary same strain, but initial colonization Lactobacillus physically remodels through production glycan-rich secretion favor unrelated commensals Acetobacter genus. Our results provide mechanistic framework understanding establishment multi-species intestinal microbiome.

Язык: Английский

---

Tools for the Microbiome: Nano and Beyond

ACS Nano, Год журнала: 2015, Номер 10(1), С. 6 - 37

Опубликована: Дек. 22, 2015

The microbiome presents great opportunities for understanding and improving the world around us elucidating interactions that compose it. also poses tremendous challenges mapping manipulating entangled networks of among myriad diverse organisms. Here, we describe opportunities, technical needs, potential approaches to address these challenges, based on recent upcoming advances in measurement control at nanoscale beyond. These needs will provide basis advancing largely descriptive studies theoretical mechanistic understandings underpin discipline engineering. We anticipate new tools methods developed be more broadly useful environmental monitoring, medicine, forensics, other areas.

Язык: Английский

---

Symbiotic organs shaped by distinct modes of genome evolution in cephalopods

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(8), С. 3030 - 3035

Опубликована: Янв. 11, 2019

Microbes have been critical drivers of evolutionary innovation in animals. To understand the processes that influence origin specialized symbiotic organs, we report sequencing and analysis genome Euprymna scolopes , a model cephalopod with richly characterized host–microbe interactions. We identified large-scale genomic reorganization shared between E. Octopus bimaculoides posit this has contributed to evolution complexity. reveal signatures host–symbiont interactions, focused on two organs : light organ, which harbors monoculture Vibrio fischeri accessory nidamental gland (ANG), reproductive organ containing bacterial consortium. Our findings suggest within originated by different mechanisms. Transcripts expressed these microbe-associated tissues displayed their own unique both coding sequences surrounding regulatory regions. Compared other tissues, showed an abundance genes associated immunity mediating light, whereas ANG was enriched orphan known only from . Together, analyses provide evidence for patterns single host.

Язык: Английский

---

Motile cilia create fluid-mechanical microhabitats for the active recruitment of the host microbiome

Proceedings of the National Academy of Sciences, Год журнала: 2017, Номер 114(36), С. 9510 - 9516

Опубликована: Авг. 23, 2017

We show that mucociliary membranes of animal epithelia can create fluid-mechanical microenvironments for the active recruitment specific microbiome host. In terrestrial vertebrates, these tissues are typically colonized by complex consortia and inaccessible to observation. Such be directly examined in aquatic animals, providing valuable opportunities analysis activity relation bacteria recruitment. Using squid-vibrio model system, we provide a characterization initial engagement microbial symbionts along ciliated tissues. Specifically, developed an empirical theoretical framework conduct census cell types, structural maps, resolve spatiotemporal flow dynamics. Our multiscale analyses revealed two distinct, highly organized populations cilia on host An array long ([Formula: see text]25 [Formula: text]m) with metachronal beat creates focuses bacteria-sized particles, at exclusion larger into sheltered zones; there, field randomly beating short text]10 mixes local fluid environment, which contains biochemical signals known prime colonization. This cilia-mediated process represents previously unrecognized mechanism symbiont Each surface recruits such as case described here is likely have system-specific features. However, all surfaces subject same physical biological constraints imposed environment evolutionary conserved structure cilia. As such, our study promises insight universal mechanisms drive symbiotic partners.

Язык: Английский

---

Host-associated microbiomes drive structure and function of marine ecosystems

PLoS Biology, Год журнала: 2019, Номер 17(11), С. e3000533 - e3000533

Опубликована: Ноя. 11, 2019

The significance of symbioses between eukaryotic hosts and microbes extends from the organismal to ecosystem level underpins health Earth's most threatened marine ecosystems. Despite rapid growth in research on host-associated microbes, individual microbial symbionts consortia significantly relevant taxa, little is known about their interactions with vast majority host species. We outline priorities strengthen our current knowledge host-microbiome how they shape argue that such advances will help predict responses species, communities, ecosystems stressors driven by human activity inform future management strategies.

Язык: Английский

---

Eco-evolutionary Dynamics Linked to Horizontal Gene Transfer in Vibrios

Annual Review of Microbiology, Год журнала: 2018, Номер 72(1), С. 89 - 110

Опубликована: Июнь 13, 2018

Vibrio is a genus of ubiquitous heterotrophic bacteria found in aquatic environments. Although they are small percentage the these environments, vibrios can predominate during blooms. Vibrios also play important roles degradation polymeric substances, such as chitin, and other biogeochemical processes. be free-living bacteria, attached to particles, or associated with organisms mutualistic, commensal, pathogenic relationship. This review focuses on vibrio ecology genome plasticity, which confers an ability adapt new niches driven, at least part, by horizontal gene transfer (HGT). The extent HGT its role pathogen emergence discussed based genomic studies environmental vibrios, mobile genetically encoded virulence factors, mechanistic different modes HGT.

Язык: Английский

---

A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host

Nature Reviews Microbiology, Год журнала: 2021, Номер 19(10), С. 654 - 665

Опубликована: Июнь 4, 2021

Язык: Английский

---

The microbiota influences the Drosophila melanogaster life history strategy

Molecular Ecology, Год журнала: 2019, Номер 29(3), С. 639 - 653

Опубликована: Дек. 21, 2019

Abstract Organisms are locally adapted when members of a population have fitness advantage in one location relative to conspecifics other geographies. For example, across latitudinal gradients, some organisms may trade off between traits that maximize components one, but not both, somatic maintenance or reproductive output. Latitudinal gradients life history strategies traditionally attributed environmental selection on an animal's genotype, without any consideration the possible impact associated microorganisms (“microbiota”) traits. Here, we show Drosophila melanogaster , key model for studying local adaptation and strategy, excluding microbiota from definitions is major shortfall. First, reveal isogenic fly line reared with different bacteria varies investment early reproduction versus maintenance. Next, wild fruit flies, abundance these same was correlated latitude strategy suggesting geographic specificity composition. Variation composition D. could be both environment host genetic selection. Finally, by eliminating manipulating lines collected gradient, genotype contributes latitude‐specific independent variation can suppress reverse differences lines. Together, findings establish animal as essential adaptation.

Язык: Английский

---

All living cells are cognitive

Biochemical and Biophysical Research Communications, Год журнала: 2020, Номер 564, С. 134 - 149

Опубликована: Сен. 22, 2020

Язык: Английский

---