Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population DOI Open Access
Nancy Weiland‐Bräuer, Sven C. Neulinger,

Nicole Pinnow

et al.

Applied and Environmental Microbiology, Journal Year: 2015, Volume and Issue: 81(17), P. 6038 - 6052

Published: June 27, 2015

The scyphozoan Aurelia aurita is recognized as a key player in marine ecosystems and driver of ecosystem change. It thus intensely studied to address ecological questions, although its associations with microorganisms remain so far undescribed. In the present study, microbiota associated A. was visualized fluorescence situ hybridization (FISH) analysis, community structure analyzed respect different life stages, compartments, populations by 16S rRNA gene amplicon sequencing. We demonstrate that composition generally highly distinct from communities ambient water. Comparison microbial developmental stages reveals evidence for stage-specific patterns. Significant restructuring during strobilation benthic polyp planktonic present, arguing course metamorphosis. Furthermore, compartments adult medusa (exumbrella mucus gastric cavity) display significant differences, indicating body part-specific colonization. A novel Mycoplasma strain identified both compartment-specific most likely inside epithelium indicated FISH analysis polyps, potential endosymbiosis. Finally, comparison polyps kept under same controlled laboratory conditions water showed population-specific patterns, due genetic background host. conclusion, presented data indicate may play important functional roles, e.g., cycle.

Language: Английский

A lasting symbiosis: how the Hawaiian bobtail squid finds and keeps its bioluminescent bacterial partner DOI
Spencer V. Nyholm, Margaret McFall‐Ngai

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(10), P. 666 - 679

Published: June 4, 2021

Language: Английский

Citations

117

A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota DOI Creative Commons
Ren Dodge, Eric W. Jones, Haolong Zhu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 21, 2023

Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level stabilizing their colonization. Primary colonizers saturate exclude secondary same strain, but initial colonization Lactobacillus physically remodels through production glycan-rich secretion favor unrelated commensals Acetobacter genus. Our results provide mechanistic framework understanding establishment multi-species intestinal microbiome.

Language: Английский

Citations

45

Tools for the Microbiome: Nano and Beyond DOI Creative Commons
Julie S. Biteen, Paul C. Blainey, Zoë G. Cardon

et al.

ACS Nano, Journal Year: 2015, Volume and Issue: 10(1), P. 6 - 37

Published: Dec. 22, 2015

The microbiome presents great opportunities for understanding and improving the world around us elucidating interactions that compose it. also poses tremendous challenges mapping manipulating entangled networks of among myriad diverse organisms. Here, we describe opportunities, technical needs, potential approaches to address these challenges, based on recent upcoming advances in measurement control at nanoscale beyond. These needs will provide basis advancing largely descriptive studies theoretical mechanistic understandings underpin discipline engineering. We anticipate new tools methods developed be more broadly useful environmental monitoring, medicine, forensics, other areas.

Language: Английский

Citations

140

Symbiotic organs shaped by distinct modes of genome evolution in cephalopods DOI Creative Commons
Mahdi Belcaid, Giorgio Casaburi, Sarah J. McAnulty

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(8), P. 3030 - 3035

Published: Jan. 11, 2019

Microbes have been critical drivers of evolutionary innovation in animals. To understand the processes that influence origin specialized symbiotic organs, we report sequencing and analysis genome Euprymna scolopes , a model cephalopod with richly characterized host–microbe interactions. We identified large-scale genomic reorganization shared between E. Octopus bimaculoides posit this has contributed to evolution complexity. reveal signatures host–symbiont interactions, focused on two organs : light organ, which harbors monoculture Vibrio fischeri accessory nidamental gland (ANG), reproductive organ containing bacterial consortium. Our findings suggest within originated by different mechanisms. Transcripts expressed these microbe-associated tissues displayed their own unique both coding sequences surrounding regulatory regions. Compared other tissues, showed an abundance genes associated immunity mediating light, whereas ANG was enriched orphan known only from . Together, analyses provide evidence for patterns single host.

Language: Английский

Citations

140

Motile cilia create fluid-mechanical microhabitats for the active recruitment of the host microbiome DOI Open Access
Janna Nawroth, Hanliang Guo, Eric J. Koch

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2017, Volume and Issue: 114(36), P. 9510 - 9516

Published: Aug. 23, 2017

We show that mucociliary membranes of animal epithelia can create fluid-mechanical microenvironments for the active recruitment specific microbiome host. In terrestrial vertebrates, these tissues are typically colonized by complex consortia and inaccessible to observation. Such be directly examined in aquatic animals, providing valuable opportunities analysis activity relation bacteria recruitment. Using squid-vibrio model system, we provide a characterization initial engagement microbial symbionts along ciliated tissues. Specifically, developed an empirical theoretical framework conduct census cell types, structural maps, resolve spatiotemporal flow dynamics. Our multiscale analyses revealed two distinct, highly organized populations cilia on host An array long ([Formula: see text]25 [Formula: text]m) with metachronal beat creates focuses bacteria-sized particles, at exclusion larger into sheltered zones; there, field randomly beating short text]10 mixes local fluid environment, which contains biochemical signals known prime colonization. This cilia-mediated process represents previously unrecognized mechanism symbiont Each surface recruits such as case described here is likely have system-specific features. However, all surfaces subject same physical biological constraints imposed environment evolutionary conserved structure cilia. As such, our study promises insight universal mechanisms drive symbiotic partners.

Language: Английский

Citations

115

Host-associated microbiomes drive structure and function of marine ecosystems DOI Creative Commons
Laetitia Wilkins, Matthieu Leray, Aaron O’Dea

et al.

PLoS Biology, Journal Year: 2019, Volume and Issue: 17(11), P. e3000533 - e3000533

Published: Nov. 11, 2019

The significance of symbioses between eukaryotic hosts and microbes extends from the organismal to ecosystem level underpins health Earth's most threatened marine ecosystems. Despite rapid growth in research on host-associated microbes, individual microbial symbionts consortia significantly relevant taxa, little is known about their interactions with vast majority host species. We outline priorities strengthen our current knowledge host-microbiome how they shape argue that such advances will help predict responses species, communities, ecosystems stressors driven by human activity inform future management strategies.

Language: Английский

Citations

114

Eco-evolutionary Dynamics Linked to Horizontal Gene Transfer in Vibrios DOI Open Access
Frédérique Le Roux, Melanie Blokesch

Annual Review of Microbiology, Journal Year: 2018, Volume and Issue: 72(1), P. 89 - 110

Published: June 13, 2018

Vibrio is a genus of ubiquitous heterotrophic bacteria found in aquatic environments. Although they are small percentage the these environments, vibrios can predominate during blooms. Vibrios also play important roles degradation polymeric substances, such as chitin, and other biogeochemical processes. be free-living bacteria, attached to particles, or associated with organisms mutualistic, commensal, pathogenic relationship. This review focuses on vibrio ecology genome plasticity, which confers an ability adapt new niches driven, at least part, by horizontal gene transfer (HGT). The extent HGT its role pathogen emergence discussed based genomic studies environmental vibrios, mobile genetically encoded virulence factors, mechanistic different modes HGT.

Language: Английский

Citations

105

A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host DOI
Karen L. Visick, Eric V. Stabb, Edward G. Ruby

et al.

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(10), P. 654 - 665

Published: June 4, 2021

Language: Английский

Citations

98

The microbiota influences the Drosophila melanogaster life history strategy DOI

Amber Walters,

Rachel C. Hughes,

Tanner B. Call

et al.

Molecular Ecology, Journal Year: 2019, Volume and Issue: 29(3), P. 639 - 653

Published: Dec. 21, 2019

Abstract Organisms are locally adapted when members of a population have fitness advantage in one location relative to conspecifics other geographies. For example, across latitudinal gradients, some organisms may trade off between traits that maximize components one, but not both, somatic maintenance or reproductive output. Latitudinal gradients life history strategies traditionally attributed environmental selection on an animal's genotype, without any consideration the possible impact associated microorganisms (“microbiota”) traits. Here, we show Drosophila melanogaster , key model for studying local adaptation and strategy, excluding microbiota from definitions is major shortfall. First, reveal isogenic fly line reared with different bacteria varies investment early reproduction versus maintenance. Next, wild fruit flies, abundance these same was correlated latitude strategy suggesting geographic specificity composition. Variation composition D. could be both environment host genetic selection. Finally, by eliminating manipulating lines collected gradient, genotype contributes latitude‐specific independent variation can suppress reverse differences lines. Together, findings establish animal as essential adaptation.

Language: Английский

Citations

86

All living cells are cognitive DOI
James A. Shapiro

Biochemical and Biophysical Research Communications, Journal Year: 2020, Volume and Issue: 564, P. 134 - 149

Published: Sept. 22, 2020

Language: Английский

Citations

70