International Journal of Biological Macromolecules, Год журнала: 2024, Номер 274, С. 133286 - 133286
Опубликована: Июнь 21, 2024
Язык: Английский
Biomaterials, Год журнала: 2024, Номер 306, С. 122478 - 122478
Опубликована: Янв. 21, 2024
Язык: Английский
Процитировано
23
Biomaterials, Год журнала: 2024, Номер 306, С. 122502 - 122502
Опубликована: Фев. 6, 2024
Extracellular vesicles (EVs) from cultured cells or bodily fluids have been demonstrated to show therapeutic value following myocardial infarction. However, challenges in donor variation, EV generation and isolation methods, material availability hindered their use. Here, we that human clinical-grade platelet concentrates a blood establishment can be used rapidly generate high concentrations of purity EVs sero-converted lysate (SCPL-EVs) with minimal processing, using size-exclusion chromatography. Processing removed serum carrier proteins, coagulation factors complement proteins the original resultant SCPL-EVs carried range trophic multiple recognised cardioprotective miRNAs. As such, protected rodent cardiomyocytes hypoxia/re-oxygenation injury stimulated angiogenesis cardiac microvessel endothelial cells. In mouse model infarction reperfusion, SCPL-EV delivery echo-guided intracavitary percutaneous injection produced large improvements function, reduced scar formation promoted angiogenesis. Since platelet-based biomaterials are already widely clinically, believe this therapy could suitable for clinical trial.
Язык: Английский
Процитировано
17
Trends in biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
2
Biomedicines, Год журнала: 2024, Номер 12(7), С. 1586 - 1586
Опубликована: Июль 17, 2024
Fibroblasts are typical mesenchymal cells widely distributed throughout the human body where they (1) synthesise and maintain extracellular matrix, ensuring structural role of soft connective tissues; (2) secrete cytokines growth factors; (3) communicate with each other cell types, acting as signalling source for stem niches; (4) involved in tissue remodelling, wound healing, fibrosis, cancer. This review focuses on developmental heterogeneity dermal fibroblasts, their ability to sense changes biomechanical properties surrounding aging, skin repair, pathologic conditions tumour development. Moreover, we describe use fibroblasts different models (e.g., vivo animal vitro systems from 2D 6D cultures) bioengineering informative potential high-throughput assays study under disease contexts personalized healthcare regenerative medicine applications.
Язык: Английский
Процитировано
15
Journal of Orthopaedic Translation, Год журнала: 2025, Номер 51, С. 1 - 12
Опубликована: Янв. 19, 2025
Язык: Английский
Процитировано
1
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Фев. 22, 2024
Gastric cancer (GC) is a malignant neoplasm originating from the epithelial cells of gastric mucosa. The pathogenesis GC intricately linked to tumor microenvironment within which reside. Tumor-associated macrophages (TAMs) primarily differentiate peripheral blood monocytes and can be broadly categorized into M1 M2 subtypes. M2-type TAMs have been shown promote growth, tissue remodeling, angiogenesis. Furthermore, they actively suppress acquired immunity, leading poorer prognosis reduced tolerance chemotherapy. Exosomes, contain myriad biologically active molecules including lipids, proteins, mRNA, noncoding RNAs, emerged as key mediators communication between TAMs. exchange these via exosomes markedly influence consequently impact progression. Recent studies elucidated correlation various clinicopathological parameters GC, such size, differentiation, infiltration depth, lymph node metastasis, TNM staging, highlighting pivotal role in development metastasis. In this review, we aim comprehensively examine bidirectional TAMs, implications alterations on immune escape, invasion, metastasis targeted therapeutic approaches for efficacy potential drug resistance strategies.
Язык: Английский
Процитировано
6
Journal of Experimental Orthopaedics, Год журнала: 2024, Номер 11(2)
Опубликована: Апрель 1, 2024
Abstract Intact articular cartilage plays a vital role in joint homeostasis. Local repairs, where defects the matrix are filled and sealed to congruity, therefore important treatments restore equilibrium. The base for all repairs is cells; either chondrocytes or chondrogeneic cells from bone, synovia fat tissue. surgical options include bone marrow stimulation techniques alone augmented with scaffolds, cell implantations osteochondral auto‐ allografts. current trend choose one‐stage procedures being easier use regulatory point of view. This narrative review provides an overview nonoperative available repair various lesions. Level Evidence IV.
Язык: Английский
Процитировано
6
APL Bioengineering, Год журнала: 2024, Номер 8(2)
Опубликована: Май 6, 2024
Human platelet lysates (HPLs) from allogeneic concentrates (PCs) are biomaterials, which rich in various trophic factors, increasingly used regenerative medicine and biotherapy. Understanding how preparation methods influence the HPL protein profile, biological function, clinical outcomes is crucial. Our study sheds light on proteomes functionality of different HPLs, with aim advancing their scientifically grounded applications. To achieve this, PCs suspended plasma underwent three distinct processing methods, resulting seven types. We characterization techniques: label-free proteomics tandem mass tag (TMT)-based quantitative proteomics, both before after immunodepletion abundant proteins. Bioinformatic tools assessed proteome, western blotting validated our data. Subsequent pre-clinical studies fluorescent labeling were as a proof concept for brain diffusion. findings revealed 1441 proteins detected using method, 952 TMT experiment depletion, 1114 subsequent depleted HPLs. Most cytoplasmic, playing key roles catalysis, hemostasis, immune responses. Notably, methodologies significantly influenced compositions, canonical pathways, and, consequently, functionality. Each exhibited specific proteins, providing valuable insight tailored Immunoblotting results selected corroborated The diffusion differential effects to hippocampus neuroprotective administered intranasally mice demonstrated. This advances understanding suggesting ways standardize customize production better efficacy Proteomic analyses also offered objective evidence that HPPL, upon intranasal delivery, not only effectively diffuses but alters expression mice, bolstering its potential treatment memory impairments.
Язык: Английский
Процитировано
6
Small, Год журнала: 2024, Номер 20(46)
Опубликована: Авг. 7, 2024
Abstract Wound healing is a dynamic process involving the timely transition of organized phases. However, infected wounds often experience prolonged inflammation due to microbial overload. Thus, addressing viable treatment needs across different stages critical challenge in wound management. Herein, novel core‐shell microneedle (CSMN) patch designed for sequential delivery tannic acid‐magnesium (TA‐Mg) complexes and extracellular vesicles from Lactobacillus druckerii (LDEVs). Upon application sites, CSMN@TA‐Mg/LDEV releases TA‐Mg first counteract pathogenic overload reduce reactive oxygen species (ROS), aiding proliferative phase. Subsequently, sustained release LDEVs enhances activities keratinocytes fibroblasts, promotes vascularization, modulates collagen deposition. Notably, track composition demonstrates that can both inhibit aggressive pathogen increase diversity at sites. Functional analysis further highlights potential facilitating skin barrier restoration. Moreover, it confirmed accelerate closure improve post‐recovery quality murine wound. Conclusively, this innovative CSMN offers rapid high‐quality alternative emphasizes significance homeostasis.
Язык: Английский
Процитировано
6
Journal of Biomedical Science, Год журнала: 2024, Номер 31(1)
Опубликована: Сен. 5, 2024
The burgeoning field of regenerative medicine has significantly advanced with recent findings on biotherapies using human platelet lysates (HPLs), derived from clinical-grade concentrates (PCs), for treating brain disorders. These developments have opened new translational research avenues to explore the neuroprotective effects platelet-extracellular vesicles (PEVs). Their potential in managing neurodegenerative conditions like traumatic injury (TBI) and Parkinson's disease (PD) warrants further exploration. We aimed here characterize composition a PEV preparation isolated concentrate (PC) supernatant, determine its neurorestorative cellular animal models TBI PD.
Язык: Английский
Процитировано
6