Bioprocessing of human platelet concentrates to generate lysates and extracellular vesicles for therapeutic applications DOI Creative Commons
Wei-Ting Yeh,

Ezrin Yi-Ling Yu,

Ya-Hsuan Lu

et al.

MethodsX, Journal Year: 2024, Volume and Issue: 13, P. 102822 - 102822

Published: July 9, 2024

This work describes protocols for preparing specific forms of human platelet lysates from pooled concentrates (PCs) and the isolation platelet-derived extracellular vesicles (p-EVs). Clinical-grade PCs can be sourced blood establishments immediately following expiration transfusion use. Here, we describe methods to process into which p-EVs isolated. Each lysate type is prepared using activation processing produce distinct products that may useful in different applications. For example, serum-converted (SCPL)-EVs were recently shown have powerful therapeutic properties myocardial infarction mice. EVs isolated all size exclusion chromatography, producing pure consistent multiple batches. Together, these allow with excellent potential clinical preclinical applications.•Platelet obtained local are reliable sustainable sources generate biomaterials.•We outline five generation one method vesicle isolation.•Each form has biological suitable certain

Language: Английский

Platelet-derived drug delivery systems: Pioneering treatment for cancer, cardiovascular diseases, infectious diseases, and beyond DOI

Yalan Zhu,

Lingling Xu, Yong Kang

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 306, P. 122478 - 122478

Published: Jan. 21, 2024

Language: Английский

Citations

23
Extracellular vesicles purified from serum-converted human platelet lysates offer strong protection after cardiac ischaemia/reperfusion injury DOI Creative Commons

Dora Livkisa,

Tzu-Hsin Chang,

Thierry Burnouf

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 306, P. 122502 - 122502

Published: Feb. 6, 2024

Extracellular vesicles (EVs) from cultured cells or bodily fluids have been demonstrated to show therapeutic value following myocardial infarction. However, challenges in donor variation, EV generation and isolation methods, material availability hindered their use. Here, we that human clinical-grade platelet concentrates a blood establishment can be used rapidly generate high concentrations of purity EVs sero-converted lysate (SCPL-EVs) with minimal processing, using size-exclusion chromatography. Processing removed serum carrier proteins, coagulation factors complement proteins the original resultant SCPL-EVs carried range trophic multiple recognised cardioprotective miRNAs. As such, protected rodent cardiomyocytes hypoxia/re-oxygenation injury stimulated angiogenesis cardiac microvessel endothelial cells. In mouse model infarction reperfusion, SCPL-EV delivery echo-guided intracavitary percutaneous injection produced large improvements function, reduced scar formation promoted angiogenesis. Since platelet-based biomaterials are already widely clinically, believe this therapy could suitable for clinical trial.

Language: Английский

Citations

17
Platelet extracellular vesicles-loaded hydrogel bandages for personalized wound care DOI
Sabine Szunerits, Andrew E.‐Y. Chuang, Jen‐Chang Yang

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
The Role of Fibroblasts in Skin Homeostasis and Repair DOI Creative Commons
Federica Boraldi, Francesco Demetrio Lofaro,

Susanna Bonacorsi

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1586 - 1586

Published: July 17, 2024

Fibroblasts are typical mesenchymal cells widely distributed throughout the human body where they (1) synthesise and maintain extracellular matrix, ensuring structural role of soft connective tissues; (2) secrete cytokines growth factors; (3) communicate with each other cell types, acting as signalling source for stem niches; (4) involved in tissue remodelling, wound healing, fibrosis, cancer. This review focuses on developmental heterogeneity dermal fibroblasts, their ability to sense changes biomechanical properties surrounding aging, skin repair, pathologic conditions tumour development. Moreover, we describe use fibroblasts different models (e.g., vivo animal vitro systems from 2D 6D cultures) bioengineering informative potential high-throughput assays study under disease contexts personalized healthcare regenerative medicine applications.

Language: Английский

Citations

15
Exosome-mediated communication between gastric cancer cells and macrophages: implications for tumor microenvironment DOI Creative Commons

Yue Qiu,

Guimei Lu,

Na Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 22, 2024

Gastric cancer (GC) is a malignant neoplasm originating from the epithelial cells of gastric mucosa. The pathogenesis GC intricately linked to tumor microenvironment within which reside. Tumor-associated macrophages (TAMs) primarily differentiate peripheral blood monocytes and can be broadly categorized into M1 M2 subtypes. M2-type TAMs have been shown promote growth, tissue remodeling, angiogenesis. Furthermore, they actively suppress acquired immunity, leading poorer prognosis reduced tolerance chemotherapy. Exosomes, contain myriad biologically active molecules including lipids, proteins, mRNA, noncoding RNAs, emerged as key mediators communication between TAMs. exchange these via exosomes markedly influence consequently impact progression. Recent studies elucidated correlation various clinicopathological parameters GC, such size, differentiation, infiltration depth, lymph node metastasis, TNM staging, highlighting pivotal role in development metastasis. In this review, we aim comprehensively examine bidirectional TAMs, implications alterations on immune escape, invasion, metastasis targeted therapeutic approaches for efficacy potential drug resistance strategies.

Language: Английский

Citations

6
Treatment of knee cartilage lesions in 2024: From hyaluronic acid to regenerative medicine DOI Creative Commons
Mats Brittberg

Journal of Experimental Orthopaedics, Journal Year: 2024, Volume and Issue: 11(2)

Published: April 1, 2024

Abstract Intact articular cartilage plays a vital role in joint homeostasis. Local repairs, where defects the matrix are filled and sealed to congruity, therefore important treatments restore equilibrium. The base for all repairs is cells; either chondrocytes or chondrogeneic cells from bone, synovia fat tissue. surgical options include bone marrow stimulation techniques alone augmented with scaffolds, cell implantations osteochondral auto‐ allografts. current trend choose one‐stage procedures being easier use regulatory point of view. This narrative review provides an overview nonoperative available repair various lesions. Level Evidence IV.

Language: Английский

Citations

6
Proteomics of human platelet lysates and insight from animal studies on platelet protein diffusion to hippocampus upon intranasal administration DOI Creative Commons
Nhi Thao Ngoc Le, Chia‐Li Han, Liling Delila

et al.

APL Bioengineering, Journal Year: 2024, Volume and Issue: 8(2)

Published: May 6, 2024

Human platelet lysates (HPLs) from allogeneic concentrates (PCs) are biomaterials, which rich in various trophic factors, increasingly used regenerative medicine and biotherapy. Understanding how preparation methods influence the HPL protein profile, biological function, clinical outcomes is crucial. Our study sheds light on proteomes functionality of different HPLs, with aim advancing their scientifically grounded applications. To achieve this, PCs suspended plasma underwent three distinct processing methods, resulting seven types. We characterization techniques: label-free proteomics tandem mass tag (TMT)-based quantitative proteomics, both before after immunodepletion abundant proteins. Bioinformatic tools assessed proteome, western blotting validated our data. Subsequent pre-clinical studies fluorescent labeling were as a proof concept for brain diffusion. findings revealed 1441 proteins detected using method, 952 TMT experiment depletion, 1114 subsequent depleted HPLs. Most cytoplasmic, playing key roles catalysis, hemostasis, immune responses. Notably, methodologies significantly influenced compositions, canonical pathways, and, consequently, functionality. Each exhibited specific proteins, providing valuable insight tailored Immunoblotting results selected corroborated The diffusion differential effects to hippocampus neuroprotective administered intranasally mice demonstrated. This advances understanding suggesting ways standardize customize production better efficacy Proteomic analyses also offered objective evidence that HPPL, upon intranasal delivery, not only effectively diffuses but alters expression mice, bolstering its potential treatment memory impairments.

Language: Английский

Citations

6
The Core‐Shell Microneedle with Probiotic Extracellular Vesicles for Infected Wound Healing and Microbial Homeostasis Restoration DOI Open Access
Fangfang Qi, Yujie Xu, Bowen Zheng

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 7, 2024

Abstract Wound healing is a dynamic process involving the timely transition of organized phases. However, infected wounds often experience prolonged inflammation due to microbial overload. Thus, addressing viable treatment needs across different stages critical challenge in wound management. Herein, novel core‐shell microneedle (CSMN) patch designed for sequential delivery tannic acid‐magnesium (TA‐Mg) complexes and extracellular vesicles from Lactobacillus druckerii (LDEVs). Upon application sites, CSMN@TA‐Mg/LDEV releases TA‐Mg first counteract pathogenic overload reduce reactive oxygen species (ROS), aiding proliferative phase. Subsequently, sustained release LDEVs enhances activities keratinocytes fibroblasts, promotes vascularization, modulates collagen deposition. Notably, track composition demonstrates that can both inhibit aggressive pathogen increase diversity at sites. Functional analysis further highlights potential facilitating skin barrier restoration. Moreover, it confirmed accelerate closure improve post‐recovery quality murine wound. Conclusively, this innovative CSMN offers rapid high‐quality alternative emphasizes significance homeostasis.

Language: Английский

Citations

6
Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson’s disease models DOI Creative Commons
Liling Delila, Ouada Nebie, Nhi Thao Ngoc Le

et al.

Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 31(1)

Published: Sept. 5, 2024

The burgeoning field of regenerative medicine has significantly advanced with recent findings on biotherapies using human platelet lysates (HPLs), derived from clinical-grade concentrates (PCs), for treating brain disorders. These developments have opened new translational research avenues to explore the neuroprotective effects platelet-extracellular vesicles (PEVs). Their potential in managing neurodegenerative conditions like traumatic injury (TBI) and Parkinson's disease (PD) warrants further exploration. We aimed here characterize composition a PEV preparation isolated concentrate (PC) supernatant, determine its neurorestorative cellular animal models TBI PD.

Language: Английский

Citations

6
Platelet-Derived Extracellular Vesicles: A New-Generation Nanostructured Tool for Chronic Wound Healing DOI
Abdolreza Esmaeilzadeh, Pegah Moharrami Yeganeh, Mahdis Nazari

et al.

Nanomedicine, Journal Year: 2024, Volume and Issue: 19(10), P. 915 - 941

Published: March 6, 2024

Chronic nonhealing wounds pose a serious challenge to regaining skin function and integrity. Platelet-derived extracellular vesicles (PEVs) are nanostructured particles with the potential promote wound healing since they can enhance neovascularization cell migration reduce inflammation scarring. This work provides an innovative overview of technical laboratory issues in PEV production, PEVs' role chronic benefits challenges its clinical translation. The article also explores proper sourcing, extraction techniques storage conditions, discusses necessity further evaluations combinational therapeutics, including dressing biomaterials, M2-derived exosomes, mesenchymal stem cells-derived microneedle technology, boost their therapeutic efficacy as advanced strategies for healing.

Language: Английский

Citations

5