Research status and challenges of plant-derived exosome-like nanoparticles

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 174, С. 116543 - 116543

Опубликована: Апрель 11, 2024

In recent years, there has been an increasing number of related studies on exosomes. Most have focused exosomes derived from mammals, confirming the important role that play in cell communication. Plants, as a natural ingredient, plant-derived confirmed to similar structures and functions mammalian-derived Plant-derived exosome-like nanoparticles (PELNs) are lipid bilayer membrane nanovesicles containing bioactive constituents such miRNA, mRNA, protein, lipids obtained plant cells, can participate intercellular communication mediate transboundary communication, high bioavailability low immunogenicity, relatively safe, shown maintaining homeostasis preventing, treating variety diseases. this review, we describe biogenesis, isolation purification methods, structural composition, stability, safety, function PELNs challenges. The anti-inflammatory, antioxidant, antitumor drug delivery mainly described, status research exosome Chinese herbal medicines is outlined. Overall, summarized importance latest results field provided theoretical basis for future clinical application PELNs.

Язык: Английский

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Natural lipid nanoparticles extracted from Morus nigra L. leaves for targeted treatment of hepatocellular carcinoma via the oral route

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 3, 2024

Abstract The clinical application of conventional medications for hepatocellular carcinoma treatment has been severely restricted by their adverse effects and unsatisfactory therapeutic effectiveness. Inspired the concept ‘medicine food homology’, we extracted purified natural exosome-like lipid nanoparticles (LNPs) from black mulberry ( Morus nigra L.) leaves. obtained MLNPs possessed a desirable hydrodynamic particle size (162.1 nm), uniform distribution (polydispersity index = 0.025), negative surface charge (-26.6 mv). These LNPs were rich in glycolipids, functional proteins, active small molecules (e.g., rutin quercetin 3- O -glucoside). In vitro experiments revealed that preferentially internalized liver tumor cell lines via galactose receptor-mediated endocytosis, increased intracellular oxidative stress, triggered mitochondrial damage, resulting suppressing viability, migration, invasion these cells. Importantly, vivo investigations suggested oral entered into circulatory system mainly through jejunum colon, they exhibited negligible superior anti-liver outcomes direct killing intestinal microbiota modulation. findings collectively demonstrate potential as natural, safe, robust nanomedicine carcinoma. Graphic abstract

Язык: Английский

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Bioactive compounds and biological functions of medicinal plant-derived extracellular vesicles

Pharmacological Research, Год журнала: 2024, Номер 200, С. 107062 - 107062

Опубликована: Янв. 9, 2024

Extracellular vesicles (EVs) are tiny lipid bilayer-enclosed membrane particles released from a variety of cell types into the surrounding environment. These EVs have massive participated in cell-to-cell communication and interspecies communication. In recent years, plant-derived extracellular (PDEVs) "exosome-like" populations found distinct plants attracted widespread attention. Especially, research on medicinal (MPDEVs) increasing, which considered kind promising natural compound. This review summarizes current knowledge MPDEVs terms bioactive compounds, including small RNA, protein, lipid, metabolite, been surface and/or lumen MPDEVs. Moreover, both vitro vivo experiments shown that exert broad biomedical functions, such as anti-inflammatory, anticancer, antioxidant, modulate microbiota, etc. may be better substitute than animal-derived (ADEVs) because safety biocompatibility future.

Язык: Английский

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Plant-Derived Exosome-Like Nanoparticles: Emerging Nanosystems for Enhanced Tissue Engineering

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 1189 - 1204

Опубликована: Фев. 1, 2024

Abstract: Tissue engineering holds great potential for tissue repair and rejuvenation. Plant-derived exosome-like nanoparticles (ELNs) have recently emerged as a promising avenue in engineering. However, there is an urgent need to understand how plant ELNs can be therapeutically applied clinical disease management, especially regeneration. In this review, we comprehensively examine the properties, characteristics, isolation techniques of ELNs. We also discuss their impact on immune system, compatibility with human body, role To ensure suitability engineering, explore various modification strategies. Additionally, provide insights into progress commercialization industrial perspectives This review aims highlight regenerative medicine by exploring current research landscape key findings. Keywords: plant, nanoparticles, nanomedicine, cartilage, wound, bone

Язык: Английский

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Harnessing tomato-derived small extracellular vesicles as drug delivery system for cancer therapy

Future Science OA, Год журнала: 2025, Номер 11(1)

Опубликована: Фев. 7, 2025

Aim This study aims to explore a sustainable and scalable approach using tomato fruit-derived sEVs (TsEVs) deliver calcitriol for enhanced anticancer effects, addressing challenges of low yield high costs associated with mammalian cell-derived sEVs.

Язык: Английский

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Edible plant-derived extracellular vesicles serve as promising therapeutic systems

Nano TransMed, Год журнала: 2023, Номер 2(2-3), С. 100004 - 100004

Опубликована: Сен. 1, 2023

Extracellular vesicles (EVs), which are natural nanocarriers characterized by a phospholipid bilayer structure, released living cells. They play crucial role in the intercellular transport of proteins, nucleic acids, lipids, and metabolites, facilitating substance delivery information exchange between In light recent numerous studies, EVs has been found to transcend their basic as mere vehicle. Instead, they demonstrate an impressive array biological activities, displaying preventive therapeutic potential mitigating various pathological processes encompassing cancer, neoplastic proliferation, infectious diseases, oxidative trauma. Particularly, derived from edible plants (EPDEVs) have emphasized for extensive range physiological regulatory functions animals humans, with targeted drug through oral administration. Leveraging these advantages, EPDEVs expected excellent competitiveness clinical applications or healthcare products. This review provides brief overview biogenesis, composition EPDEVs, summarizes mechanisms. It also analyzes methods isolating purifying plant-EVs, assessing advantages disadvantages; discusses latest advancements biomedical applications, concludes prospective insight into research development directions EPDEVs.

Язык: Английский

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Ginger: a representative material of herb-derived exosome-like nanoparticles

Frontiers in Nutrition, Год журнала: 2023, Номер 10

Опубликована: Июль 13, 2023

Edible plant-derived exosome-like nanoparticles (PELNs) provide numerous benefits, including high yield, low cost, ethical compatibility, and multiple health which enable them to address technical constraints associated with mammalian nanoparticles. Herbs, known for their abundant bioactive components, are considered the primary source of natural medicines within plant kingdom. Recently, a number herbaceous sources have been investigated isolation functionality (ELNs). However, they commonly referred as PELNs, distinct pharmacological properties overlooked. In this review, these herb-derived ELNs designated HELNs, novel herbal product that may also exhibit superior activity compared other types PELNs. Among documented ginger-derived (GELNs) most extensively studied. This review employs GELNs an exemplar delineate process extraction purification, together physical biochemical characteristics therapeutic potential. The aim is promote development application future research encouraged uncover additional properties, extending beyond those GELNs.

Язык: Английский

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Gut‐liver axis: Potential mechanisms of action of food‐derived extracellular vesicles

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(6)

Опубликована: Июнь 1, 2024

Abstract Food‐derived extracellular vesicles (FEVs) are nanoscale membrane obtained from dietary materials such as breast milk, plants and probiotics. Distinct other EVs, FEVs can survive the harsh degrading conditions in gastrointestinal tract reach intestines. This unique feature allows to be promising prebiotics health oral nanomedicine for gut disorders, inflammatory bowel disease. Interestingly, therapeutic effects of have recently also been observed non‐gastrointestinal diseases. However, mechanisms remain unclear or even mysterious. It is speculated that orally administered could enter bloodstream, remote organs, thus exert therein. emerging evidence suggests amount reaching organs beyond marginal may insufficient account significant achieved regarding diseases involving liver. Thus, we herein propose primarily act locally intestine by modulating intestinal microenvironments barrier integrity microbiota, thereby eliciting impact remotely on liver via gut‐liver axis. Likewise, drugs delivered system through As main metabolic hub, microenvironment implicated In fact, many patients with non‐alcoholic fatty disease, obesity, diabetes cardiovascular disease suffer a leaky dysbiosis. this review, provide an overview recent progress discuss their biomedical applications agents drug delivery systems, highlighting pivotal role axis action treatment disorders

Язык: Английский

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Extracellular vesicles and exosome-like nanovesicles as pioneering oral drug delivery systems

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 11

Опубликована: Янв. 8, 2024

As extracellular vesicle (EV)-based nanotechnology has developed rapidly, it made unprecedented opportunities for nanomedicine possible. EVs and exosome-like nanovesicles (ELNVs) are natural nanocarriers with unique structural, compositional, morphological characteristics that provide excellent physical, chemical, biochemical properties. In this literature, we examine the of EVs, including how they administered orally their therapeutic activity. According to current examples ELNVs oral delivery, milk plant can exert effects through protein, nucleic acid, lipid components. Furthermore, several methods loading drugs into exosomes targeting have been employed investigate capability. Moreover, discuss as potential drug carriers role disease prevention treatment or in future. conclusion, issues associated development from sources such plants, well concerns standardized applications these discussed.

Язык: Английский

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Emerging Drug Delivery Vectors: Engineering of Plant-Derived Nanovesicles and Their Applications in Biomedicine

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 2591 - 2610

Опубликована: Март 1, 2024

Extracellular vesicles can transmit intercellular information and transport biomolecules to recipient cells during various pathophysiological processes in the organism. Animal cell exosomes have been identified as potential nanodrugs delivery vehicles, yet they some shortcomings such high immunogenicity, cytotoxicity, complicated preparation procedures. In addition exosomes, plant-derived extracellular (PDVs), which carry a variety of active substances, are another promising nano-transport vehicles emerging recent years due their stable physicochemical properties, wide source, low cost. This work briefly introduces collection characterization PDVs, then focuses on application PDVs natural or engineered drug carriers biomedicine, finally discusses development challenges future applications.

Язык: Английский

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