Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 197, С. 114826 - 114826
Опубликована: Апрель 23, 2023
Язык: Английский
Cells, Год журнала: 2023, Номер 12(10), С. 1416 - 1416
Опубликована: Май 17, 2023
Extracellular vesicles (EVs) such as ectosomes and exosomes have gained attention promising natural carriers for drug delivery. Exosomes, which range from 30 to 100 nm in diameter, possess a lipid bilayer are secreted by various cells. Due their high biocompatibility, stability, low immunogenicity, favored cargo carriers. The membrane of also offers protection against degradation, making them desirable candidate However, loading into remains be challenge. Despite strategies incubation, electroporation, sonication, extrusion, freeze–thaw cycling, transfection that been developed facilitate loading, inadequate efficiency still persists. This review an overview current delivery using summarizes recent approaches small-molecule, nucleic acid, protein drugs exosomes. With insights these studies, we provide ideas more efficient effective molecules
Язык: Английский
Процитировано
160
ACS Biomaterials Science & Engineering, Год журнала: 2023, Номер 9(2), С. 577 - 594
Опубликована: Янв. 9, 2023
Exosomes are the phospholipid-membrane-bound subpopulation of extracellular vesicles derived from plasma membrane. The main activity exosomes is cellular communication. In cancer, play an important rolefrom two distinct perspectives, one related to carcinogenesis and other as theragnostic drug delivery tools. outer phospholipid membrane Exosome improves targeting efficiency. . Some vital features such biocompatibility, low toxicity, immunogenicity make it a more exciting system. Exosome-based new innovative approach cancer treatment. Exosome-associated biomarker analysis heralded era diagnostics in specific way. This Review focuses on exosome biogenesis, sources, isolation, interrelationship with exosome-related biomarkers, loading methods, exosome-based biomolecule delivery, advances limitations clinical settings studies. understanding will change diagnostic therapeutic future.
Язык: Английский
Процитировано
139
Biomedicines, Год журнала: 2021, Номер 9(6), С. 594 - 594
Опубликована: Май 24, 2021
The accumulation of extracellular β-amyloid (Aβ) plaques within the brain is unique to Alzheimer’s disease (AD) and thought induce synaptic deficits neuronal loss. Optimal therapies should tackle core AD pathophysiology prevent decline in memory cognitive functions. This study aimed evaluate therapeutic performance mesenchymal stem cell-derived exosomes (MSC-exosomes), which are secreted membranous elements encapsulating a variety MSC factors, on AD. A human neural cell culture model with familial (FAD) mutations was established co-cultured purified MSC-exosomes. 2-[18F]Fluoro-2-deoxy-d-glucose ([18F]FDG) novel object recognition (NOR) testing were performed before/after treatment effect vivo. AD-related pathology expression memory/synaptic plasticity-related genes also evaluated. results showed that MSC-exosomes reduced Aβ restored model. [18F]FDG-PET imaging assessment revealed significant improvement glucose metabolism function transgenic mice. phase neurons astrocytes mice found be regulated after Our demonstrates mechanism provides an alternative strategy based cell-free for
Язык: Английский
Процитировано
117
Biomarker Research, Год журнала: 2022, Номер 10(1)
Опубликована: Май 12, 2022
Abstract Exosomes, ranging in size from 30 to 150 nm as identified initially via electron microscopy 1946, are one of the extracellular vesicles (EVs) produced by many cells and have been subject studies; initially, they were considered cell wastes with belief that exosomes maintain homeostasis. Nowadays, it has found EVs secreted different play a vital role cellular communication usually both physiological pathological conditions. Due presence markers ligands on surface exosomes, paracrine, endocrine autocrine effects some cases. Immune cells, like other can secrete interact surrounding these vesicles. system cells-derived (IEXs) induce responses, such increasing decreasing transcription various genes regulating cytokine production. This review deliberate function innate acquired immune derived their pathogenesis diseases, therapeutic appliances.
Язык: Английский
Процитировано
102
Molecules, Год журнала: 2022, Номер 27(21), С. 7289 - 7289
Опубликована: Окт. 27, 2022
Currently, particular interest among the scientific community is focused on exploring use of exosomes for several pharmaceutical and biomedical applications. This due to identification role as an excellent intercellular communicator by delivering requisite cargo comprising functional proteins, metabolites nucleic acids. Exosomes are smallest extracellular vesicles (EV) with sizes ranging from 30–100 nm derived endosomes. have similar surface morphology cells act a signal transduction channel between cells. They encompass different biomolecules, such acids lipids, thus rendering them naturally attractive drug delivery vehicle. Like other advanced systems, polymeric nanoparticles liposomes encapsulate substances, also gained much attention in enhancing therapeutic activity. present many advantages, compatibility living tissues, low toxicity, extended blood circulation, capability pass contents one cell another, non-immunogenic special targeting various cells, making carrier. Exosome-based molecules still early stages research clinical trials. The problems transition issues related exosome-based drugs need be overcome using tools better understanding systemic evaluation exosomes. In this current review, we summarize most up-to-date knowledge about complex biological journey biogenesis secretion, isolation techniques, characterization, loading methods, applications, challenges future perspectives
Язык: Английский
Процитировано
102
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Июль 22, 2023
Abstract Ocular drug delivery has constantly challenged ophthalmologists and scientists due to various anatomical physiological barriers. Static dynamic ocular barriers prevent the entry of exogenous substances impede therapeutic agents' active absorption. This review elaborates on anatomy eye associated constraints. Followed by an illustration some common diseases, including glaucoma their current clinical therapies, emphasizing significance therapy in treating diseases. Subsequently, advances modalities, especially nanotechnology-based systems, are recommended, typical research is highlighted. Based related research, systematic comprehensive characterizations nanocarriers summarized, hoping assist with future research. Besides, we summarize ophthalmic drugs currently market or still trials recent patents nanocarriers. Finally, inspired trends concepts, provide insight into challenges faced novel systems further put forward directions for We hope this can inspiration motivation better design development formulations. Graphical abstract
Язык: Английский
Процитировано
100
Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(4), С. 877 - 889
Опубликована: Апрель 5, 2024
Язык: Английский
Процитировано
99
Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(4), С. 836 - 849
Опубликована: Апрель 1, 2024
Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.
Язык: Английский
Процитировано
97
Advanced Healthcare Materials, Год журнала: 2023, Номер 12(13)
Опубликована: Фев. 5, 2023
Abstract Myocardial infarction (MI) is a cardiovascular disease that poses serious threat to human health. Uncontrolled and excessive cardiac fibrosis after MI has been recognized as primary contributor mortality by heart failure. Thus, prevention of or alleviation progression important for repair. To this end, biocompatible microneedle (MN) patch based on gelatin fabricated load exosomes containing microRNA‐29b (miR‐29b) mimics with antifibrotic activity prevent MI. Exosomes are isolated from umbilical cord mesenchymal stem cells loaded miR‐29b via electroporation, which can be internalized effectively in fibroblasts upregulate the expression downregulate fibrosis‐related proteins. After being implanted infarcted mouse model, MN increase retention myocardium, leading inflammation, reduction infarct size, inhibition fibrosis, improvement function. This design explored suitable platform deliver biomolecules locally showing potential treatment clinical applications.
Язык: Английский
Процитировано
91
Acta Pharmaceutica Sinica B, Год журнала: 2022, Номер 12(10), С. 3822 - 3842
Опубликована: Май 11, 2022
Extracellular vesicles (EVs) are secreted by both eukaryotes and prokaryotes, present in all biological fluids of vertebrates, where they transfer DNA, RNA, proteins, lipids, metabolites from donor to recipient cells cell-to-cell communication. Some EV components can also indicate the type status their parent serve as diagnostic targets for liquid biopsy. EVs natively carry or be modified contain therapeutic agents (e.g., nucleic acids, polysaccharides, small molecules) physical, chemical, bioengineering strategies. Due excellent biocompatibility stability, ideal nanocarriers bioactive ingredients induce signal transduction, immunoregulation, other effects, which targeted specific cell types. Herein, we review classification, intercellular communication, isolation, characterization strategies apply therapeutics. This focuses on recent advances applications carriers vitro research towards vivo animal models early clinical applications, using representative examples fields cancer chemotherapeutic drug, vaccine, infectious disease vaccines, regenerative medicine gene therapy. Finally, discuss current challenges therapeutics future development.
Язык: Английский
Процитировано
90