Angiogenesis,
Год журнала:
2024,
Номер
27(3), С. 423 - 440
Опубликована: Май 6, 2024
Abstract
Background
Retinopathy
of
prematurity
(ROP),
which
often
presents
with
bronchopulmonary
dysplasia
(BPD),
is
among
the
most
common
morbidities
affecting
extremely
premature
infants
and
a
leading
cause
severe
vision
impairment
in
children
worldwide.
Activations
inflammasome
cascade
microglia
have
been
implicated
playing
role
development
both
ROP
BPD.
Apoptosis-associated
speck-like
protein
containing
caspase
recruitment
domain
(ASC)
pivotal
assembly.
Utilizing
mouse
models
oxygen-induced
retinopathy
(OIR)
BPD,
this
study
was
designed
to
test
hypothesis
that
hyperoxia
induces
ASC
speck
formation,
leads
microglial
activation
retinopathy,
inhibition
formation
by
humanized
monoclonal
antibody,
IC100,
directed
against
ASC,
will
ameliorate
abnormal
retinal
vascular
formation.
Methods
We
first
tested
retina
ASC-citrine
reporter
mice
expressing
fusion
C-terminal
citrine
(fluorescent
GFP
isoform)
using
BPD
model
causes
lung
eye
injury
exposing
newborn
room
air
(RA)
or
85%
O
2
from
postnatal
day
(P)
1
P14.
The
retinas
were
dissected
on
P14
flat
mounts
used
detect
endothelium
AF-594-conjugated
isolectin
B4
(IB4)
citrine-tagged
specks.
To
assess
effects
IC100
an
OIR
model,
wildtype
(C57BL/6
J)
exposed
RA
P1
P6,
then
75%
P7
P11,
P12
P18.
At
randomized
following
groups:
placebo
PBS
(RA-PBS),
(O
-PBS),
+
intravitreal
injection
-IC100-IVT),
intraperitoneal
-IC100-IP).
Retinal
vascularization
evaluated
mount
staining
IB4.
Microglial
detected
immunofluorescence
for
allograft
inflammatory
factor
(AIF-1)
CD206.
structure
analyzed
H&E-stained
sections,
function
pattern
electroretinography
(PERG).
RNA-sequencing
(RNA-seq)
performed
determine
transcriptional
treatment
OIR.
Results
specks
significantly
increased
exposure
colocalized
vasculature
models,
associated
activation.
Treatment
IC100-IVT
IC100-IP
reduced
vaso-obliteration
neovascularization.
also
activation,
restored
structure,
improved
function.
RNA-seq
showed
corrected
induction
genes
angiogenesis,
leukocyte
migration,
VEGF
signaling
caused
.
suppression
cell
junction
assembly,
neuron
projection,
recognition
Conclusion
These
data
demonstrate
crucial
pathogenesis
efficacy
therapeutic
anti-ASC
antibody
treating
mice.
Thus,
may
potentially
be
considered
diseases
oxygen
stresses
such
as
ROP.
Cell Death and Disease,
Год журнала:
2023,
Номер
14(2)
Опубликована: Фев. 9, 2023
Abstract
Pyroptosis
is
a
recently
described
mechanism
of
programmed
cell
death
mediated
by
proteins
the
gasdermin
family.
Widely
recognized
signaling
cascades
include
classical,
non-classical,
caspase-3-dependent
E
and
caspase-8-dependent
D
pathways.
Additional
pyroptotic
pathways
have
been
subsequently
reported.
With
rising
prevalence
advanced
age,
role
pyroptosis
in
degenerative
diseases
elderly
has
attracted
increased
research
attention.
This
article
reviews
primary
mechanisms
summarizes
progress
such
as
presbycusis,
age-related
macular
degeneration,
Alzheimer’s
disease,
intervertebral
disc
osteoarthritis.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(7), С. 6317 - 6317
Опубликована: Март 28, 2023
Cataracts
remain
the
first
or
second
leading
cause
of
blindness
in
all
world
regions.
In
diabetic
population,
cataracts
not
only
have
a
3–5
times
higher
incidence
than
healthy
population
but
also
affect
people
at
younger
age.
patients
with
type
1
diabetes,
occur
on
average
20
years
earlier
non-diabetic
population.
addition,
risk
developing
increases
duration
diabetes
and
poor
metabolic
control.
A
better
understanding
mechanisms
to
formation
enables
more
effective
treatment
holistic
approach
patient.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 10, 2023
Adult
hippocampal
neurogenesis
generates
functional
neurons
from
neural
progenitor
cells
in
the
dentate
gyrus
(DG)
to
complement
and
repair
circuits,
thus
benefiting
treatment
of
depression.
Increasing
evidence
has
shown
that
aberrant
microglial
activity
can
disrupt
appropriate
formation
development
properties
neurogenesis,
which
will
play
a
crucial
role
occurrence
However,
mechanisms
crosstalk
between
microglia
adult
depression
are
not
yet
fully
understood.
Therefore,
this
review,
we
first
introduce
recent
discoveries
regarding
roles
etiology
Then,
systematically
discuss
possible
how
regulate
according
studies,
involve
toll-like
receptors,
polarization,
fractalkine-C-X3-C
motif
chemokine
receptor
1,
hypothalamic-pituitary-adrenal
axis,
cytokines,
brain-derived
neurotrophic
factor,
microbiota-gut-brain
etc.
In
addition,
summarize
promising
drugs
could
improve
by
regulating
microglia.
These
findings
help
us
understand
complicated
pathological
shed
light
on
new
strategies
for
disease.
Abstract
Programmed
cell
death
(PCD)
is
regarded
as
a
pathological
form
of
with
an
intracellular
program
mediated,
which
plays
pivotal
role
in
maintaining
homeostasis
and
embryonic
development.
Pyroptosis
new
paradigm
PCD,
has
received
increasing
attention
due
to
its
close
association
immunity
disease.
inflammatory
mediated
by
gasdermin
that
promotes
the
release
proinflammatory
cytokines
contents
induced
inflammasome
activation.
Recently,
evidence
studies
shows
pyroptosis
crucial
conditions
like
cardiovascular
diseases
(CVDs),
cancer,
neurological
(NDs),
metabolic
(MDs),
suggesting
targeting
potential
intervention
for
treatment
these
diseases.
Based
on
this,
review
aims
identify
molecular
mechanisms
signaling
pathways
related
activation
summarizes
current
insights
into
complicated
relationship
between
multiple
human
(CVDs,
NDs,
MDs).
We
also
discuss
promising
novel
strategy
method
treating
focus
pathway
application
clinics.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Ноя. 28, 2023
Abstract
As
a
naturally
occurring
cytolytic
peptide,
melittin
(MLT)
not
only
exhibits
potent
direct
tumor
cell-killing
effect
but
also
possesses
various
immunomodulatory
functions.
MLT
shows
minimal
chances
for
developing
resistance
and
has
been
recognized
as
promising
broad-spectrum
antitumor
drug
because
of
this
unique
dual
mechanism
action.
However,
still
displays
obvious
toxic
side
effects
during
treatment,
such
nonspecific
activity,
hemolytic
toxicity,
coagulation
disorders,
allergic
reactions,
seriously
hampering
its
broad
clinical
applications.
With
thorough
research
on
mechanisms
the
rapid
development
nanotechnology,
significant
effort
devoted
to
shielding
against
toxicity
achieving
tumor-directed
delivery
improve
therapeutic
efficacy
MLT.
Herein,
we
mainly
summarize
potential
recent
progress
in
targeted
strategies
therapy,
passive
targeting,
active
targeting
stimulus-responsive
targeting.
Additionally,
highlight
prospects
challenges
realizing
full
field
therapy.
By
exploring
molecular
MLT,
comprehensive
review
may
inspire
new
ideas
multimechanism
synergistic
Progress in Retinal and Eye Research,
Год журнала:
2024,
Номер
101, С. 101263 - 101263
Опубликована: Апрель 23, 2024
Retinal
diseases
encompass
various
conditions
associated
with
sight-threatening
immune
responses
and
are
leading
causes
of
blindness
worldwide.
These
include
age-related
macular
degeneration,
diabetic
retinopathy,
glaucoma
uveitis.
Emerging
evidence
underscores
the
vital
role
innate
response
in
retinal
diseases,
beyond
previously
emphasized
T-cell-driven
processes
adaptive
system.
In
particular,
pyroptosis,
a
newly
discovered
programmed
cell
death
process
involving
inflammasome
formation,
has
been
implicated
loss
membrane
integrity
release
inflammatory
cytokines.
Several
disease-relevant
animal
models
have
provided
that
formation
inflammasomes
induction
pyroptosis
cells
contribute
to
inflammation
diseases.
this
review
article,
we
summarize
current
knowledge
about
system
We
also
provide
insights
into
translational
targeting
approaches,
including
novel
drugs
countering
improve
diagnosis
treatment
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(1), С. 731 - 731
Опубликована: Янв. 1, 2023
The
research
on
new
treatments
for
dry
eye
diseases
(DED)
has
exponentially
grown
over
the
past
decades.
increased
prevalence
of
conditions,
particularly
in
younger
population,
received
much
attention.
Therefore,
it
is
utmost
importance
to
identify
novel
therapeutical
targets.
Regulated
cell
death
(RCD)
an
essential
process
control
biological
homeostasis
tissues
and
organisms.
identification
different
mechanisms
RCD
stimulated
their
involvement
human
pathologies.
Whereas
apoptosis
been
widely
studied
DED
included
vicious
cycle,
role
still
needs
be
completely
elucidated.
In
this
review,
we
will
explore
potential
roles
types
ocular
surface
dysfunction.
Starting
from
evidence
oxidative
stress
inflammation
pathology,
analyse
therapeutic
applications
following
principal
mechanisms:
ferroptosis,
necroptosis,
pyroptosis.
