Frontiers in Cell and Developmental Biology,
Год журнала:
2021,
Номер
9
Опубликована: Авг. 3, 2021
Osteoarthritis
(OA)
is
a
common
chronic
disease
and
significant
health
concern
that
needs
to
be
urgently
solved.
OA
affects
the
cartilage
entire
joint
tissues,
including
subchondral
bone,
synovium,
infrapatellar
fat
pads.
The
physiological
pathological
changes
in
these
tissues
affect
occurrence
development
of
OA.
Understanding
complex
crosstalk
among
different
their
roles
initiation
progression
critical
elucidating
pathogenic
mechanism
In
this
review,
we
begin
with
an
overview
role
chondrocytes,
synovial
cells
(synovial
fibroblasts
macrophages),
mast
cells,
osteoblasts,
osteoclasts,
various
stem
engineered
(induced
pluripotent
cells)
pathogenesis.
Then,
discuss
mechanisms
by
which
communicate,
paracrine
signaling,
local
microenvironment,
co-culture,
extracellular
vesicles
(exosomes),
cell
tissue
engineering.
We
particularly
focus
on
therapeutic
potential
clinical
applications
cell-derived
vesicles,
serve
as
modulators
cell-to-cell
communication,
field
regenerative
medicine,
such
repair.
Finally,
challenges
limitations
related
exosome-based
treatment
for
are
discussed.
This
article
provides
comprehensive
summary
key
might
targets
future
therapies
Bioactive Materials,
Год журнала:
2023,
Номер
30, С. 169 - 183
Опубликована: Авг. 4, 2023
Osteoarthritis
(OA)
is
the
most
common
disabling
joint
disease
with
no
effective
modifying
drugs.
Extracellular
vesicles
released
by
several
types
of
mesenchymal
stem
cells
could
promote
cartilage
repair
and
ameliorate
OA
pathology
in
animal
models,
representing
a
novel
therapeutic
strategy.
In
this
study,
we
demonstrated
that
extracellular
derived
from
human
umbilical
cord
(hUC-EVs)
maintain
chondrocyte
homeostasis
alleviate
OA,
further
revealed
molecular
mechanism
effect.
miR-223,
which
directly
bind
3'UTR
NLRP3
mRNA,
was
found
to
be
key
miRNA
for
hUC-EVs
exert
beneficial
effects
on
inflammation
inhibiting
protecting.
For
enhancing
effect
mitigating
osteoarthritis,
exogenous
miR-223
loaded
into
electroporation,
collagen
II-targeting
peptide
(WYRGRL)
modified
onto
surface
genetic
engineering
achieve
more
targeted
efficient
RNA
delivery
cartilage.
The
dual-engineered
EVs
showed
maximal
inflammasome
activation
pyroptosis,
offered
excellent
results
treatment
OA.
This
study
provides
theoretical
basis
promising
strategy
application
engineered
treatment.
Osteoarthritis
(OA),
the
commonest
arthritis,
is
characterized
by
progressive
destruction
of
cartilage,
leading
to
disability.
The
Current
early
clinical
treatment
strategy
for
OA
often
centers
on
anti-inflammatory
or
analgesia
medication,
weight
loss,
improved
muscular
function
and
articular
cartilage
repair.
Although
these
treatments
can
relieve
symptoms,
tends
be
progressive,
most
patients
require
arthroplasty
at
terminal
stages
OA.
Recent
studies
have
shown
a
close
correlation
between
joint
pain,
inflammation,
synovial
cells.
Consequently,
understanding
potential
mechanisms
associated
with
action
cells
in
could
beneficial
management
Therefore,
this
review
comprehensively
describes
biological
functions
cells,
synovium,
together
pathological
changes
OA,
interaction
which
lacking
present
literature.
Additionally,
therapeutic
approaches
based
are
further
discussed
from
perspective,
highlighting
new
direction
ACS Nano,
Год журнала:
2024,
Номер
18(31), С. 20101 - 20110
Опубликована: Июль 23, 2024
Osteoarthritis
(OA)
is
a
prevalent
degenerative
disease
that
afflicts
more
than
250
million
people
worldwide,
impairing
their
mobility
and
quality
of
life.
However,
conventional
drug
therapy
palliative.
Exosomes
(Exo),
although
with
the
potential
to
fundamentally
repair
cartilage,
face
challenges
in
efficient
enrichment
delivery.
In
this
study,
we
developed
magnetic
polysaccharide
hydrogel
particles
as
microcarriers
for
synergistic
OA.
The
were
composed
modified
natural
polysaccharides,
hyaluronic
acid
(HAMA),
chondroitin
sulfate
(CSMA),
generated
from
microfluidic
electrospray
combination
cryogelation
process.
Magnetic
nanoparticles
spiny
structures
capable
capturing
stem
cell
Exo
encapsulated
within
together
an
anti-inflammatory
diclofenac
sodium
(DS).
released
DS
had
effect
alleviating
OA
symptoms
promoting
cartilage
repair.
vitro
vivo
results
demonstrated
excellent
performance
microcarrier
treatment.
We
believe
work
has
other
related
diseases.
Journal of Cellular and Molecular Medicine,
Год журнала:
2020,
Номер
24(18), С. 10855 - 10865
Опубликована: Авг. 9, 2020
Curcumin
treatment
was
reported
to
delay
the
progression
of
OA,
but
its
underlying
mechanism
remains
unclear.
In
this
study,
we
aimed
investigate
molecular
role
curcumin
in
OA
treatment.
Accordingly,
by
conducting
MTT
and
flow
cytometry
assays,
found
that
exosomes
derived
from
curcumin-treated
MSCs
helped
maintain
viability
while
inhibiting
apoptosis
model
cells.
Additionally,
quantitative
real-time
PCR
Western
blot
assays
showed
significantly
restored
down-regulated
miR-143
miR-124
expression
as
well
up-regulated
NF-kB
ROCK1
Mechanistically,
decreased
DNA
methylation
promoters.
addition,
3'
UTRs
were
proven
contain
binding
sites
for
miR-124,
respectively.
Therefore,
up-regulation
cellular
mouse
models
treated
with
remarkably
normal
ROCK1.
Consequently,
attenuated
exosomes.
Our
results
clarified
therapeutic
MSC-derived
Frontiers in Cell and Developmental Biology,
Год журнала:
2020,
Номер
8
Опубликована: Фев. 21, 2020
Parkinson's
disease
(PD)
is
the
second
most
prevalent
neurodegenerative
in
world,
after
Alzheimer's
(AD),
affecting
approximately
1%
of
people
over
65
years
age.
Exosomes
were
once
considered
to
be
cellular
waste
and
functionless.
However,
our
understanding
about
exosome
function
has
increased,
exosomes
have
been
found
carry
specific
proteins,
lipids,
functional
messenger
RNAs
(mRNAs),
high
amounts
non-coding
(including
microRNAs,
lincRNAs,
circRNAs)
other
bioactive
substances.
shown
involved
many
physiological
processes
vivo,
including
intercellular
communication,
cell
migration,
angiogenesis,
anti-tumor
immunity.
Moreover,
may
pivotal
occurrence
progression
various
diseases.
Therefore,
several
diverse
potential
applications
due
their
unique
structure
function.
For
instance,
used
as
biological
markers
for
diagnosis
prognosis
diseases,
or
a
natural
carrier
drugs
clinical
treatment.
Here,
we
review
roles
pathogenesis,
diagnosis,
treatment,
PD.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2020,
Номер
8
Опубликована: Окт. 29, 2020
Osteoarthritis
(OA)
has
gradually
been
recognized
as
a
low-grade
inflammatory
state.
Inflammatory
infiltration
of
synovium
by
macrophages,
T
cells,
B
cells
and
other
immune
are
often
exhibited
in
OA
patient,
which
play
key
role
the
pathogenesis
OA.
Hence,
orchestrating
local
microenvironment
tissue
regeneration
is
important
to
treatment
MSCs
offer
advantages
cartilage
due
their
effective
immunomodulatory
properties
anti-inflammatory
abilities.
The
paracrine
effect
mediated
exosomes
MSCs,
suggested
for
curative
recent
years.
In
this
review,
we
summarize
interactions
mechanism
or
MSC-derived
with
OA-related
how
relates
therapeutic
effects
osteoarthritis.
Additionally,
discuss
potential
MSC-exosomes
novel
cell-free
therapy
clinical
Acta Biomaterialia,
Год журнала:
2020,
Номер
114, С. 31 - 52
Опубликована: Июль 8, 2020
In
the
absence
of
timely
and
proper
treatments,
injuries
to
articular
cartilage
(AC)
can
lead
degeneration
ultimately
result
in
osteoarthritis.
Regenerative
medicine
tissue
engineering
techniques
are
emerging
as
promising
approaches
for
AC
regeneration
repair.
Although
use
cell-seeded
scaffolds
prior
implantation
regenerate
repair
lesions
some
extent,
these
still
restricted
by
limited
cell
sources,
excessive
costs,
risks
disease
transmission
complex
manufacturing
practices.
Recently
developed
acellular
scaffold
that
rely
on
recruitment
endogenous
cells
injured
sites
avoid
drawbacks
offer
great
promise
situ
regeneration.
Multiple
stem/progenitor
(ESPCs)
found
joint-resident
niches
have
capability
migrate
injury
participate
However,
natural
ESPCs
is
insufficient,
local
microenvironment
hostile
after
injury.
Hence,
an
strategy
based
combination
chemoattractants
effectively
specifically
recruit
improve
may
provide
new
insights
into
This
review
provides
a
brief
overview
of:
(1)
status
strategy;
(2)
subpopulations,
potential
migration
routes
(PMRs)
their
immunomodulatory
reparative
effects;
(3)
adverse
(4)
scaffold-based
drug
delivery
systems
(SDDSs)
utilized
regeneration;
(5)
challenges
future
perspectives
has
been
investigated
several
decades,
much
work
remains
be
performed
this
field.
Future
studies
should
following
aims:
reporting
up-to-date
progress
strategies;
determining
subpopulations
ESPCs,
cellular
molecular
mechanisms
underlying
anti-inflammatory,
elucidating
enhance
ESPC
developing
advanced
SDDSs
chemoattractant
dispatch.
Herein,
we
present
systematic
aforementioned
issues
better
understanding
strategies
Journal of Neurochemistry,
Год журнала:
2020,
Номер
157(3), С. 413 - 428
Опубликована: Дек. 29, 2020
Parkinson
disease
(PD)
is
a
prevalent
neurodegenerative
disease,
in
which
the
formation
of
misfolded
and
aggregated
α-synuclein
key
neuropathological
hallmark.
Recent
studies
reveal
that
extracellular
vesicles
such
as
exosomes
present
potential
mechanism
for
propagation
pathological
throughout
brain.
The
ability
to
transport
proteins
genetic
material
between
cells,
including
mRNA
microRNAs
have
been
implicated
PD
pathology,
provides
critical
insights
how
may
contribute
progression
PD.
Advances
also
made
investigation
tools
modulation
Parkinson's
pathology;
their
detection
extracellularly
facilitate
use
biomarkers,
while
small
size
could
be
utilised
vectors
delivery
therapeutics.
aim
this
review
was
highlight
our
current
knowledge
role
clinical
application.
International Journal of Molecular Sciences,
Год журнала:
2020,
Номер
21(4), С. 1541 - 1541
Опубликована: Фев. 24, 2020
Exosomes
are
nanosized
vesicles
(30–140
nm)
of
endocytic
origin
that
play
important
roles
in
regenerative
medicine.
They
derived
from
cell
membranes
during
internalization
and
stabilize
biological
fluids
such
as
blood
synovia.
Temporomandibular
joint
osteoarthritis
(TMJ
OA)
is
a
degenerative
disease,
which,
addition
to
chronic
pain,
characterized
by
progressive
cartilage
breakdown,
condylar
bone
remodeling,
synovitis.
However,
traditional
clinical
treatments
have
limited
symptom-
structure-modifying
effects
restore
damaged
other
TMJ
tissues.
This
due
the
self-healing
capacity
cartilage.
Recently,
stem-cell-derived
exosomes
been
studied
an
alternative
therapeutic
approach
tissue
repair
regeneration.
It
known
trophic
regulation
mesenchymal
stem
cells
(MSCs)
has
anti-inflammatory
immunomodulatory
under
pathological
conditions,
research
on
MSC-derived
rapidly
accumulating.
mimic
major
MSCs.
affect
activity
immune
effector
possess
multilineage
differentiation
potential,
including
chondrogenic
osteogenic
differentiation.
Furthermore,
capable
regenerating
or
osseous
compartments
restoring
injured
tissues
can
treat
dysfunction
pain
caused
OA.
In
this
review,
we
looked
at
uniqueness
TMJ,
pathogenesis
OA,
potential
role
for
