Methods in cell biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
eLife, Год журнала: 2023, Номер 12
Опубликована: Фев. 23, 2023
Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of
Язык: Английский
Процитировано
93
Genetics, Год журнала: 2023, Номер 224(2)
Опубликована: Май 2, 2023
Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.
Язык: Английский
Процитировано
38
Cell, Год журнала: 2023, Номер 186(13), С. 2911 - 2928.e20
Опубликована: Июнь 1, 2023
Animals with complex nervous systems demand sleep for memory consolidation and synaptic remodeling. Here, we show that, although the Caenorhabditis elegans system has a limited number of neurons, is necessary both processes. In addition, it unclear if, in any system, collaborates experience to alter synapses between specific neurons whether this ultimately affects behavior. C. have defined connections well-described contributions We that spaced odor-training post-training induce long-term memory. Memory consolidation, but not acquisition, requires pair interneurons, AIYs, which play role odor-seeking worms consolidate memory, odor conditioning are required diminish inhibitory AWC chemosensory AIYs. Thus, demonstrate living organism events immediately after training drive structures.
Язык: Английский
Процитировано
31
Current Biology, Год журнала: 2023, Номер 33(19), С. 4150 - 4159.e5
Опубликована: Сен. 22, 2023
Язык: Английский
Процитировано
29
Neuron, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
11
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across
Язык: Английский
Процитировано
2
Опубликована: Фев. 27, 2025
In both invertebrates such as Drosophila and vertebrates mouse or human, the brain contains most diverse population of cell types any tissue. It is generally accepted that transcriptional diversity an early step in generating neuronal glial diversity, followed by establishment a unique gene expression profile determines morphology, connectivity, function. , there are two neural stem cells, called Type 1 (T1) 2 (T2) neuroblasts. contrast to T1 neuroblasts, T2 neuroblasts generate intermediate progenitors (INPs) expand number types. The T2-derived neurons contributes large portion central complex (CX), conserved region plays role sensorimotor integration. Recent work has revealed much connectome CX, but how this assembled remains unclear. Mapping derived from necessary linking assembly adult brain. Here we perform single nuclei RNA sequencing neuroblast-derived glia. We identify clusters containing all known classes glia, male/female enriched, 161 neuron-specific clusters. map neurotransmitter neuropeptide transcription factor combinatorial codes for each cluster (presumptive neuron subtype). This directs functional studies determine whether code specifies distinct type within CX. several columnar subtypes (NPF+ AstA+) closely related Our data support hypothesis represents one few subtypes.
Язык: Английский
Процитировано
2
Nature Aging, Год журнала: 2022, Номер 2(6), С. 494 - 507
Опубликована: Май 16, 2022
Язык: Английский
Процитировано
36
Nature Ecology & Evolution, Год журнала: 2022, Номер 6(8), С. 1191 - 1204
Опубликована: Июнь 16, 2022
Abstract Ant colonies with permanent division of labour between castes and highly distinct roles the sexes have been conceptualized to be superorganisms, but cellular molecular mechanisms that mediate caste/sex-specific behavioural specialization remained obscure. Here we characterized brain cell repertoire queens, gynes (virgin queens), workers males Monomorium pharaonis by obtaining 206,367 single-nucleus transcriptomes. In contrast Drosophila , mushroom body Kenyon cells are abundant in ants display a high diversity most subtypes being enriched worker brains, evolutionarily derived caste. Male brains as specialized opposite trends composition higher abundances all optic lobe neuronal subtypes, while gyne queen generalized, reminiscent solitary ancestors. Role differentiation from virgin inseminated queens induces abundance changes roughly 35% types, indicating active neurogenesis and/or programmed death during this transition. We also identified insemination-induced probably associated longevity fecundity reproductive caste, including increases ensheathing glia population dopamine-regulated Dh31 -expressing neurons. conclude caste extreme sex-differentiation induced major neural circuitry ants.
Язык: Английский
Процитировано
35
Neuron, Год журнала: 2022, Номер 110(14), С. 2299 - 2314.e8
Опубликована: Май 24, 2022
Transcription factors specify the fate and connectivity of developing neurons. We investigate how a lineage-specific transcription factor, Acj6, controls precise dendrite targeting Drosophila olfactory projection neurons (PNs) by regulating expression cell-surface proteins. Quantitative proteomic profiling wild-type acj6 mutant PNs in intact brains, proteome-informed genetic screen identified PN surface proteins that execute Acj6-regulated wiring decisions. These include canonical cell adhesion molecules previously not associated with wiring, such as Piezo, whose mechanosensitive ion channel activity is dispensable for its function targeting. Comprehensive analyses revealed Acj6 employs unique sets different types Combined executors rescued phenotypes higher efficacy breadth than individual executors. Thus, specificity neuron specifying distinct combinatorial
Язык: Английский
Процитировано
33