A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Language: Английский

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Visual processing in the fly, from photoreceptors to behavior

Genetics, Journal Year: 2023, Volume and Issue: 224(2)

Published: May 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Language: Английский

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Sleep is required to consolidate odor memory and remodel olfactory synapses

Cell, Journal Year: 2023, Volume and Issue: 186(13), P. 2911 - 2928.e20

Published: June 1, 2023

Animals with complex nervous systems demand sleep for memory consolidation and synaptic remodeling. Here, we show that, although the Caenorhabditis elegans system has a limited number of neurons, is necessary both processes. In addition, it unclear if, in any system, collaborates experience to alter synapses between specific neurons whether this ultimately affects behavior. C. have defined connections well-described contributions We that spaced odor-training post-training induce long-term memory. Memory consolidation, but not acquisition, requires pair interneurons, AIYs, which play role odor-seeking worms consolidate memory, odor conditioning are required diminish inhibitory AWC chemosensory AIYs. Thus, demonstrate living organism events immediately after training drive structures.

Language: Английский

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Associative learning in the box jellyfish Tripedalia cystophora

Current Biology, Journal Year: 2023, Volume and Issue: 33(19), P. 4150 - 4159.e5

Published: Sept. 22, 2023

Language: Английский

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The unbearable slowness of being: Why do we live at 10 bits/s?

Neuron, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Language: Английский

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Transcriptional complexity in the insect central complex: single nuclei RNA-sequencing of adult brain neurons derived from type 2 neuroblasts

Published: Feb. 27, 2025

In both invertebrates such as Drosophila and vertebrates mouse or human, the brain contains most diverse population of cell types any tissue. It is generally accepted that transcriptional diversity an early step in generating neuronal glial diversity, followed by establishment a unique gene expression profile determines morphology, connectivity, function. , there are two neural stem cells, called Type 1 (T1) 2 (T2) neuroblasts. contrast to T1 neuroblasts, T2 neuroblasts generate intermediate progenitors (INPs) expand number types. The T2-derived neurons contributes large portion central complex (CX), conserved region plays role sensorimotor integration. Recent work has revealed much connectome CX, but how this assembled remains unclear. Mapping derived from necessary linking assembly adult brain. Here we perform single nuclei RNA sequencing neuroblast-derived glia. We identify clusters containing all known classes glia, male/female enriched, 161 neuron-specific clusters. map neurotransmitter neuropeptide transcription factor combinatorial codes for each cluster (presumptive neuron subtype). This directs functional studies determine whether code specifies distinct type within CX. several columnar subtypes (NPF+ AstA+) closely related Our data support hypothesis represents one few subtypes.

Language: Английский

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Neuronal induction of BNIP3-mediated mitophagy slows systemic aging in Drosophila

Nature Aging, Journal Year: 2022, Volume and Issue: 2(6), P. 494 - 507

Published: May 16, 2022

Language: Английский

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A single-cell transcriptomic atlas tracking the neural basis of division of labour in an ant superorganism

Nature Ecology & Evolution, Journal Year: 2022, Volume and Issue: 6(8), P. 1191 - 1204

Published: June 16, 2022

Abstract Ant colonies with permanent division of labour between castes and highly distinct roles the sexes have been conceptualized to be superorganisms, but cellular molecular mechanisms that mediate caste/sex-specific behavioural specialization remained obscure. Here we characterized brain cell repertoire queens, gynes (virgin queens), workers males Monomorium pharaonis by obtaining 206,367 single-nucleus transcriptomes. In contrast Drosophila , mushroom body Kenyon cells are abundant in ants display a high diversity most subtypes being enriched worker brains, evolutionarily derived caste. Male brains as specialized opposite trends composition higher abundances all optic lobe neuronal subtypes, while gyne queen generalized, reminiscent solitary ancestors. Role differentiation from virgin inseminated queens induces abundance changes roughly 35% types, indicating active neurogenesis and/or programmed death during this transition. We also identified insemination-induced probably associated longevity fecundity reproductive caste, including increases ensheathing glia population dopamine-regulated Dh31 -expressing neurons. conclude caste extreme sex-differentiation induced major neural circuitry ants.

Language: Английский

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Transcription factor Acj6 controls dendrite targeting via a combinatorial cell-surface code

Neuron, Journal Year: 2022, Volume and Issue: 110(14), P. 2299 - 2314.e8

Published: May 24, 2022

Transcription factors specify the fate and connectivity of developing neurons. We investigate how a lineage-specific transcription factor, Acj6, controls precise dendrite targeting Drosophila olfactory projection neurons (PNs) by regulating expression cell-surface proteins. Quantitative proteomic profiling wild-type acj6 mutant PNs in intact brains, proteome-informed genetic screen identified PN surface proteins that execute Acj6-regulated wiring decisions. These include canonical cell adhesion molecules previously not associated with wiring, such as Piezo, whose mechanosensitive ion channel activity is dispensable for its function targeting. Comprehensive analyses revealed Acj6 employs unique sets different types Combined executors rescued phenotypes higher efficacy breadth than individual executors. Thus, specificity neuron specifying distinct combinatorial

Language: Английский

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