The unexpected PD-L1 suppression function of celery-derived extracellular vesicles improves lung cancer chemotherapy efficacy DOI Open Access
Xin Lü, Zhuang Yu, Junjun Wang

и другие.

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2024, Номер 5(4), С. 697 - 713

Опубликована: Ноя. 9, 2024

Aim: The article explores celery-derived extracellular vesicles (CDEVs), characterized by high cellular uptake, low immunogenicity, and stability, as a therapeutic strategy for antitumor nanomedicines. Methods: methods employed in this study include vitro cell experiments such co-culture, Western Blot, flow cytometry. In vivo were conducted C57BL/6 tumor-bearing mice subcutaneously injected with Lewis lung carcinoma (LLC) cells. encompassed parameters survival rate, body weight, tumor size, cytometry, immunohistochemistry, spectral live imaging system. Results: Our revealed that CDEVs could be used drugs to effectively downregulate the phosphorylated signal transducer activator of transcription 3 (p-STAT3)/programmed death ligand 1 (PD-L1) axis cancer co-culture experiments, observed impede expression PD-L1, thereby interfering interaction between PD-L1 programmed (PD-1) subsequently preventing suppression T distribution loaded paclitaxel (PTX) demonstrated better targeting capabilities. Remarkably, following CDEVs-PTX treatment, CD8+ levels increased, presumably leading improved effects. Conclusion: not only serve drug carriers but also function themselves; such, through single administration CDEVs, it is possible combine immunotherapy chemotherapy achieve effects two, providing more comprehensive effective treatment promises improve outcomes reduce adverse therapy.

Язык: Английский

Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection DOI Creative Commons
Shumei Wang, Baoye He, Huaitong Wu

и другие.

Cell Host & Microbe, Год журнала: 2023, Номер 32(1), С. 93 - 105.e6

Опубликована: Дек. 15, 2023

Cross-kingdom small RNA trafficking between hosts and microbes modulates gene expression in the interacting partners during infection. However, whether other RNAs are also transferred is unclear. Here, we discover that host plant Arabidopsis thaliana delivers mRNAs via extracellular vesicles (EVs) into fungal pathogen Botrytis cinerea. A fluorescent aptamer reporter Broccoli system reveals EVs recipient cells. Using translating ribosome affinity purification profiling polysome analysis, observe delivered translated Ectopic of two B. cinerea shows their proteins detrimental to knockout mutants genes corresponding these more susceptible. Thus, plants have a strategy reduce infection by transporting from pathogenic fungi compromise infection, providing knowledge helps combat crop diseases.

Язык: Английский

Процитировано

48
Rice extracellular vesicles send defense proteins into fungus Rhizoctonia solani to reduce disease DOI
Yifan Huang, Wei Li,

Tiangu Liu

и другие.

Developmental Cell, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

4
Edible plant-derived extracellular vesicles serve as promising therapeutic systems DOI Creative Commons
Chunhua Yang, Wenjing Zhang,

Muran Bai

и другие.

Nano TransMed, Год журнала: 2023, Номер 2(2-3), С. 100004 - 100004

Опубликована: Сен. 1, 2023

Extracellular vesicles (EVs), which are natural nanocarriers characterized by a phospholipid bilayer structure, released living cells. They play crucial role in the intercellular transport of proteins, nucleic acids, lipids, and metabolites, facilitating substance delivery information exchange between In light recent numerous studies, EVs has been found to transcend their basic as mere vehicle. Instead, they demonstrate an impressive array biological activities, displaying preventive therapeutic potential mitigating various pathological processes encompassing cancer, neoplastic proliferation, infectious diseases, oxidative trauma. Particularly, derived from edible plants (EPDEVs) have emphasized for extensive range physiological regulatory functions animals humans, with targeted drug through oral administration. Leveraging these advantages, EPDEVs expected excellent competitiveness clinical applications or healthcare products. This review provides brief overview biogenesis, composition EPDEVs, summarizes mechanisms. It also analyzes methods isolating purifying plant-EVs, assessing advantages disadvantages; discusses latest advancements biomedical applications, concludes prospective insight into research development directions EPDEVs.

Язык: Английский

Процитировано

30
Extracellular vesicles, genetic programmers DOI
Graça Raposo,

Philip D. Stahl

Nature Cell Biology, Год журнала: 2024, Номер 26(1), С. 22 - 23

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

11
Spray‐induced gene silencing to control plant pathogenic fungi: A step‐by‐step guide DOI Open Access
Sandra Mosquera, Mireille Ginésy, Irene Teresa Bocos‐Asenjo

и другие.

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

RNA interference (RNAi)-based control technologies are gaining popularity as potential alternatives to synthetic fungicides in the ongoing effort manage plant pathogenic fungi. Among these methods, spray-induced gene silencing (SIGS) emerges particularly promising due its convenience and feasibility for development. This approach is a new technology disease management, which double-stranded RNAs (dsRNAs) targeting essential or virulence genes applied plants products subsequently absorbed by pathogens, triggering effect inhibition of infection process. Spray-induced has demonstrated efficacy laboratory settings against various fungal pathogens. However, research progressed from greenhouse field environments, novel challenges arose, such ensuring stability dsRNAs their effective delivery targets. Here, we provide practical guide SIGS outlines steps considerations needed designing assessing dsRNA molecules. It also addresses key inherent SIGS, including molecules, how nanoencapsulation can aid overcoming obstacles. Additionally, underscores existing knowledge gaps that warrant further aims assistance researchers, especially those field, encouraging advancement broad range

Язык: Английский

Процитировано

1
Progress and Opportunities of In Planta and Topical RNAi for the Biotechnological Control of Agricultural Pests DOI Creative Commons
Marcos Fernando Basso,

Daniel D. Noriega Vasquez,

Eduardo Romano de Campos-Pinto

и другие.

Agronomy, Год журнала: 2025, Номер 15(4), С. 859 - 859

Опубликована: Март 29, 2025

In planta RNAi or host-induced gene silencing (HIGS) has undergone significant advancements that have rendered it efficient and stable at the transgenerational level in plants for regulating host genes targeting of insect pests plant pathogens. Similarly, topical spray-induced (SIGS) garnered considerable attention as an environmentally sustainable, selective, alternative approach to chemical control Several biotechnology companies startups focused their efforts on RNAi-based solutions application agriculture. Nevertheless, further technological are required enhance efficacy agriculture, including improved dsRNA delivery systems, better target selection, addressing biosafety regulatory issues. Herein, this review discusses key advances bottlenecks RNAi, summarizes successful applications these technologies agriculture focusing Furthermore, delves into patenting landscape, considerations, risk evaluations, current status Latin America. Finally, explores contributions science, food production, fostering a more sustainable form

Язык: Английский

Процитировано

1
Spectral flow cytometry for detecting DNA cargo in malaria parasite-derived extracellular vesicles DOI Creative Commons
Ewa Kozela, Ekaterina Petrovich-Kopitman,

Yves Berger

и другие.

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108481 - 108481

Опубликована: Апрель 1, 2025

Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules various human host systems. Yet, study this mode in research currently constrained due limitations high-resolution tools and absence commercial antibodies. Here, we demonstrate power an advanced spectral flow cytometry approach robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids DNA cargo within (non-antibody staining approach), were able subpopulation parasitic-derived enriched DNA. Furthermore, could quantitatively measure DNA-carrying two distinct stages parasite: rings trophozoites. Our findings showcase potential monitor dynamic changes nucleic acid pathogenic EVs.

Язык: Английский

Процитировано

1
Development and Evaluation of Reconstructed Nanovesicles from Turmeric for Multifaceted Obesity Intervention DOI
Jialin Wang, Tianyu Zhang,

Ruilan Gu

и другие.

ACS Nano, Год журнала: 2024, Номер 18(34), С. 23117 - 23135

Опубликована: Авг. 17, 2024

The escalating prevalence of obesity poses significant health challenges due to its direct association with various diseases. Most existing medications, such as appetite suppressants and fat absorption inhibitors, suffer from limited effectiveness undesirable side effects. Here, inspired by the versatile metabolic effects turmeric, we developed a naturally derived nanoformulation "Reconstructed Turmeric-derived Nanovesicles (Rec-tNVs)" for treatment. Employing quantitative nanoflow cytometry, four-orders-of-magnitude increase in curcumin content (∼10

Язык: Английский

Процитировано

5
Insights into Bacterial Extracellular Vesicle Biogenesis, Functions, and Implications in Plant–Microbe Interactions DOI Creative Commons

Sarita Pandey,

Anaïs Blache, Wafa Achouak

и другие.

Microorganisms, Год журнала: 2024, Номер 12(3), С. 532 - 532

Опубликована: Март 6, 2024

Plant–microbe interactions play a crucial role in shaping plant health and survival. In recent years, the of extracellular vesicles (EVs) mediating intercellular communication between plants microbes has emerged as an intriguing area research. EVs serve important carriers bioactive molecules genetic information, facilitating cells even different organisms. Pathogenic bacteria leverage to amplify their virulence, exploiting cargo rich toxins virulence factors. Conversely, beneficial initiate EV secretion stimulate immune responses nurture symbiotic relationships. The transfer EV-packed small RNAs (sRNAs) been demonstrated facilitate modulation responses. Furthermore, harnessing potential holds promise for development innovative diagnostic tools sustainable crop protection strategies. This review highlights biogenesis functions importance defense, paves way future research this exciting field.

Язык: Английский

Процитировано

4
Applications of emerging extracellular vesicles technologies in the treatment of inflammatory diseases DOI Creative Commons

Kecheng Lou,

Hui Luo,

Xinghua Jiang

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 13, 2024

The emerging extracellular vesicles technologies is an advanced therapeutic approach showing promising potential for addressing inflammatory diseases. These techniques have been proven to positive effects on immune modulation and anti-inflammatory responses. With these advancements, a comprehensive review update the role of in diseases become timely. This aims summarize research progress vesicle such as plant-derived vesicles, milk-derived mesenchymal stem cell-derived macrophage-derived etc., treatment It elucidates their significance regulating inflammation, promoting tissue repair, treating goal provide insights future this field, fostering application development technology

Язык: Английский

Процитировано

3