Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(1), P. 7 - 8
Published: Jan. 1, 2024
Language: Английский
Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 32(1), P. 93 - 105.e6
Published: Dec. 15, 2023
Cross-kingdom small RNA trafficking between hosts and microbes modulates gene expression in the interacting partners during infection. However, whether other RNAs are also transferred is unclear. Here, we discover that host plant Arabidopsis thaliana delivers mRNAs via extracellular vesicles (EVs) into fungal pathogen Botrytis cinerea. A fluorescent aptamer reporter Broccoli system reveals EVs recipient cells. Using translating ribosome affinity purification profiling polysome analysis, observe delivered translated Ectopic of two B. cinerea shows their proteins detrimental to knockout mutants genes corresponding these more susceptible. Thus, plants have a strategy reduce infection by transporting from pathogenic fungi compromise infection, providing knowledge helps combat crop diseases.
Language: Английский
Citations
47
Developmental Cell, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
2
Nano TransMed, Journal Year: 2023, Volume and Issue: 2(2-3), P. 100004 - 100004
Published: Sept. 1, 2023
Extracellular vesicles (EVs), which are natural nanocarriers characterized by a phospholipid bilayer structure, released living cells. They play crucial role in the intercellular transport of proteins, nucleic acids, lipids, and metabolites, facilitating substance delivery information exchange between In light recent numerous studies, EVs has been found to transcend their basic as mere vehicle. Instead, they demonstrate an impressive array biological activities, displaying preventive therapeutic potential mitigating various pathological processes encompassing cancer, neoplastic proliferation, infectious diseases, oxidative trauma. Particularly, derived from edible plants (EPDEVs) have emphasized for extensive range physiological regulatory functions animals humans, with targeted drug through oral administration. Leveraging these advantages, EPDEVs expected excellent competitiveness clinical applications or healthcare products. This review provides brief overview biogenesis, composition EPDEVs, summarizes mechanisms. It also analyzes methods isolating purifying plant-EVs, assessing advantages disadvantages; discusses latest advancements biomedical applications, concludes prospective insight into research development directions EPDEVs.
Language: Английский
Citations
30
Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(1), P. 22 - 23
Published: Jan. 1, 2024
Language: Английский
Citations
10
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
RNA interference (RNAi)-based control technologies are gaining popularity as potential alternatives to synthetic fungicides in the ongoing effort manage plant pathogenic fungi. Among these methods, spray-induced gene silencing (SIGS) emerges particularly promising due its convenience and feasibility for development. This approach is a new technology disease management, which double-stranded RNAs (dsRNAs) targeting essential or virulence genes applied plants products subsequently absorbed by pathogens, triggering effect inhibition of infection process. Spray-induced has demonstrated efficacy laboratory settings against various fungal pathogens. However, research progressed from greenhouse field environments, novel challenges arose, such ensuring stability dsRNAs their effective delivery targets. Here, we provide practical guide SIGS outlines steps considerations needed designing assessing dsRNA molecules. It also addresses key inherent SIGS, including molecules, how nanoencapsulation can aid overcoming obstacles. Additionally, underscores existing knowledge gaps that warrant further aims assistance researchers, especially those field, encouraging advancement broad range
Language: Английский
Citations
1
Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 859 - 859
Published: March 29, 2025
In planta RNAi or host-induced gene silencing (HIGS) has undergone significant advancements that have rendered it efficient and stable at the transgenerational level in plants for regulating host genes targeting of insect pests plant pathogens. Similarly, topical spray-induced (SIGS) garnered considerable attention as an environmentally sustainable, selective, alternative approach to chemical control Several biotechnology companies startups focused their efforts on RNAi-based solutions application agriculture. Nevertheless, further technological are required enhance efficacy agriculture, including improved dsRNA delivery systems, better target selection, addressing biosafety regulatory issues. Herein, this review discusses key advances bottlenecks RNAi, summarizes successful applications these technologies agriculture focusing Furthermore, delves into patenting landscape, considerations, risk evaluations, current status Latin America. Finally, explores contributions science, food production, fostering a more sustainable form
Language: Английский
Citations
1
Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108481 - 108481
Published: April 1, 2025
Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules various human host systems. Yet, study this mode in research currently constrained due limitations high-resolution tools and absence commercial antibodies. Here, we demonstrate power an advanced spectral flow cytometry approach robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids DNA cargo within (non-antibody staining approach), were able subpopulation parasitic-derived enriched DNA. Furthermore, could quantitatively measure DNA-carrying two distinct stages parasite: rings trophozoites. Our findings showcase potential monitor dynamic changes nucleic acid pathogenic EVs.
Language: Английский
Citations
1
ACS Nano, Journal Year: 2024, Volume and Issue: 18(34), P. 23117 - 23135
Published: Aug. 17, 2024
The escalating prevalence of obesity poses significant health challenges due to its direct association with various diseases. Most existing medications, such as appetite suppressants and fat absorption inhibitors, suffer from limited effectiveness undesirable side effects. Here, inspired by the versatile metabolic effects turmeric, we developed a naturally derived nanoformulation "Reconstructed Turmeric-derived Nanovesicles (Rec-tNVs)" for treatment. Employing quantitative nanoflow cytometry, four-orders-of-magnitude increase in curcumin content (∼10
Language: Английский
Citations
5
Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 532 - 532
Published: March 6, 2024
Plant–microbe interactions play a crucial role in shaping plant health and survival. In recent years, the of extracellular vesicles (EVs) mediating intercellular communication between plants microbes has emerged as an intriguing area research. EVs serve important carriers bioactive molecules genetic information, facilitating cells even different organisms. Pathogenic bacteria leverage to amplify their virulence, exploiting cargo rich toxins virulence factors. Conversely, beneficial initiate EV secretion stimulate immune responses nurture symbiotic relationships. The transfer EV-packed small RNAs (sRNAs) been demonstrated facilitate modulation responses. Furthermore, harnessing potential holds promise for development innovative diagnostic tools sustainable crop protection strategies. This review highlights biogenesis functions importance defense, paves way future research this exciting field.
Language: Английский
Citations
4
Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Abstract Probiotics have been established to exert a positive impact on the treatment of various diseases. Indeed, these active microorganisms garnered significant attention in recent years for their potential prevent and treat illnesses. Their beneficial effects hypothesized be linked released extracellular vesicles. These nanoscale structures, secreted during growth metabolism probiotics, possess favorable biocompatibility targeting properties, thereby promoting intercellular material transport signaling. This article aimed review bioactive components functions probiotics vesicles, highlighting role diseases discussing future applications. By exploring mechanisms probiotic vesicles disease development, this provide theoretical reference further research therapeutic potential. © 2025 Society Chemical Industry.
Language: Английский
Citations
0