In
this
study,
a
novel
oral
formulation
of
berberine
hydrochloride
(BBH)
hydrogel
was
successfully
synthesized
through
physical
cross-linking
using
chitosan
(CS)
and
carboxymethyl-β-cyclodextrin
(CMCD).
The
characterization
results
confirmed
the
successful
synthesis
CS/CMCD
subsequent
loading
BBH
into
composite
(CS/CMCD/BBH)
effectively
accomplished.
used
as
model
drug
resulting
demonstrated
sustained
release
profile.
addition
to
its
improved
solubility
characteristics,
exhibited
excellent
antibacterial
activity
against
common
pathogens
such
Escherichia
coli
(E.
coli),
Staphylococcus
aureus
(S.
aureus),
Candida
albicans
(C.
albicans).
Additionally,
in
vitro
studies
indicated
that
not
cytotoxic
NIH3T3
HaCaT
cells,
suggesting
safety
for
biomedical
applications.
This
lack
effects,
combined
with
mechanical
strength,
improvements,
properties
hydrogel,
positions
CS/CMCD/BBH
promising
candidate
effective
delivery
BBH.
By
addressing
challenges
BBH,
offers
viable
solution
administration
hydrochloride,
potential
applications
various
fields.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(4), С. 2100 - 2100
Опубликована: Фев. 8, 2024
The
rising
prevalence
of
drug-resistant
bacteria
underscores
the
need
to
search
for
innovative
and
nature-based
solutions.
One
approaches
may
be
use
plants
that
constitute
a
rich
source
miscellaneous
compounds
with
wide
range
biological
properties.
This
review
explores
antimicrobial
activity
seven
bioactives
their
possible
molecular
mechanisms
action.
Special
attention
was
focused
on
antibacterial
properties
berberine,
catechin,
chelerythrine,
cinnamaldehyde,
ellagic
acid,
proanthocyanidin,
sanguinarine
against
Staphylococcus
aureus,
Enterococcus
spp.,
Klebsiella
pneumoniae,
Acinetobacter
baumannii,
Escherichia
coli,
Serratia
marcescens
Pseudomonas
aeruginosa.
growing
interest
in
novel
therapeutic
strategies
based
new
plant-derived
formulations
confirmed
by
number
articles.
Natural
products
are
one
most
promising
intensively
examined
agents
combat
consequences
overuse
misuse
classical
antibiotics.
Abstract
Stimulus‐responsive
nanocarrier
materials
have
garnered
significant
attention
in
biomedical
science.
Among
them,
metal‐organic
frameworks
(MOFs)
emerged
as
promising
candidates
due
to
their
remarkable
antibacterial
properties
and
pH
sensitivity.
In
this
study,
a
pH‐responsive
bimetallic
MOFs
carrier,
ZIF‐8@ZIF‐67,
was
successfully
synthesized
for
the
delivery
of
berberine
hydrochloride
(BBH).
Comprehensive
characterization
using
UV‐Vis
spectroscopy,
FT‐IR
X‐ray
diffraction
(XRD),
scanning
electron
microscopy
(SEM),
transmission
(TEM)
confirmed
successful
synthesis
structural
integrity
ZIF‐8@ZIF‐67
BBH@ZIF‐8@ZIF‐67.
Subsequently,
drug
release
behavior,
efficacy,
biocompatibility
composite
material
were
systematically
evaluated.
Our
findings
revealed
that
BBH@ZIF‐8@ZIF‐67
exhibited
well‐controlled
properties,
excellent
effects
biocompatibility.
Furthermore,
molecular
docking
results
penicillin‐binding
protein
2a
dihydrofolate
reductase
S.
aureus
may
serve
two
potential
targets
BBH.
This
research
provides
valuable
insights
into
use
developing
highly
efficient
stimulus‐responsive
systems
applications.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2023,
Номер
22(4), С. 2523 - 2590
Опубликована: Апрель 17, 2023
Abstract
Plant
byproducts
and
waste
present
enormous
environmental
challenges
an
opportunity
for
valorization
industrial
application.
Due
to
consumer
demands
natural
compounds,
the
evident
paucity
of
novel
antimicrobial
agents
against
foodborne
pathogens,
urgent
need
improve
arsenal
infectious
diseases
resistance
(AMR),
plant
byproduct
compounds
have
attracted
significant
research
interest.
Emerging
highlighted
their
promising
activity,
yet
inhibitory
mechanisms
remain
largely
unexplored.
Therefore,
this
review
summarizes
overall
on
activity
compounds.
A
total
315
antimicrobials
from
byproducts,
totaling
1338
minimum
concentrations
(MIC)
(in
μg/mL)
a
broad
spectrum
bacteria,
were
identified,
particular
emphasis
was
given
with
high
or
good
(typically
<100
μg/mL
MIC).
Moreover,
mechanisms,
particularly
bacterial
discussed
in‐depth,
summarizing
latest
using
combat
pathogenic
microorganisms
AMR.
Furthermore,
safety
concerns,
relevant
legislation,
perspective,
current
gaps
in
byproducts–derived
comprehensively
discussed.
This
comprehensive
covering
up‐to‐date
information
represents
powerful
tool
screening
selecting
most
sources
developing
agents.
Microbial Pathogenesis,
Год журнала:
2024,
Номер
193, С. 106774 - 106774
Опубликована: Июль 3, 2024
The
increasing
prevalence
of
extensively
drug-and
pan-drug-resistant
Pseudomonas
aeruginosa
is
a
major
concern
for
global
public
health.
Therefore,
it
crucial
to
develop
novel
antimicrobials
that
specifically
target
P.
and
its
biofilms.
In
the
present
study,
we
determined
berberine
hydrochloride
inhibited
growth
planktonic
bacteria
as
well
prevented
formation
Moreover,
observed
downregulation
in
expression
pslA
pelA
biofilm-related
genes.
Compared
with
existing
antibiotics,
exhibits
multiple
modes
action
against
aeruginosa.
Our
findings
suggest
exerts
antimicrobial
effects
by
damaging
bacterial
cell
membranes,
generating
reactive
oxygen
species
(ROS),
reducing
intracellular
adenosine
triphosphate
(ATP)
levels.
Furthermore,
showed
minimal
cytotoxicity
reduced
susceptibility
drug
resistance.
mouse
model
peritonitis,
significantly
exhibited
strong
bacteriostatic
action.
conclusion,
safe
effective
antibacterial
agent
inhibits
Polymers,
Год журнала:
2024,
Номер
16(16), С. 2368 - 2368
Опубликована: Авг. 21, 2024
In
this
study,
a
novel
oral
formulation
of
berberine
hydrochloride
(BBH)
hydrogel
was
successfully
synthesized
through
physical
cross-linking
using
chitosan
(CS)
and
carboxymethyl-β-cyclodextrin
(CMCD).
The
characterization
results
confirmed
the
successful
synthesis
CS/CMCD
subsequent
loading
BBH
into
composite
(CS/CMCD/BBH)
effectively
accomplished.
used
as
model
drug
resulting
demonstrated
sustained
release
profile.
addition
to
its
improved
solubility
characteristics,
exhibited
excellent
antibacterial
activity
against
common
pathogens
such
Escherichia
coli
(E.
coli),
Staphylococcus
aureus
(S.
aureus),
Candida
albicans
(C.
albicans).
Additionally,
in
vitro
studies
indicated
that
not
cytotoxic
NIH3T3
HaCaT
cells,
suggesting
safety
for
biomedical
applications.
This
lack
effects,
combined
with
mechanical
strength,
improvements,
properties
hydrogel,
positions
CS/CMCD/BBH
promising
candidate
effective
delivery
BBH.
By
addressing
challenges
BBH,
offers
viable
solution
administration
potential
applications
various
fields.
To
fight
the
global
epidemic
of
drug-resistant
bacteria,
essential
oils
have
gained
increasing
attention
as
a
new
source
antibiotics.
The
antimicrobial
activity
Monarda
didyma
(MDEO)
for
Carbapenem-resistant
Klebsiella
pneumoniae
(CRKP)
strains
were
determined
by
agar
disc
diffusion
assay
and
broth
microdilution
assay.
further
understand
MDEO
efficacy,
time-growth
curve
was
performed.
biofilm
formation
CRKP
crystalline
violet
staining
method,
additionally,
changes
in
intracellular
Adenosine
triphosphate
(ATP),
protein,
Alkaline
phosphatase
(AKP)
activities,
membrane
integrity
investigated
to
assess
influence
on
cell
damage.
Finally,
activities
key
enzymes
tricarboxylic
acid
(TCA)
pathways
pentose
phosphate
(PPP)
examined
determine
effect
respiratory
metabolism
CRKP.
This
study
presents
antibacterial
mechanism
against
with
minimum
inhibitory
concentration
(MIC)
bactericidal
(MBC)
1.25
mg/ml.
time-kill
kinetics
approach
evident
at
2
h
compared
control
its
MIC
2MIC.
Surface
electron
microscopic
ATP
studies
provided
evidence
multi-target
action
could
inhibit
formation.
also
cause
irreversible
damage
membrane,
resulting
leakage
biological
macromolecules
(protein,
ATP)
reduction
activities.
affected
metabolism,
such
PPP
TCA
pathways.
reduce
(Glucose-6-phosphate
dehydrogenase,
citrate
synthase,
isocitrate
α-ketoglutarate
dehydrogenase)
exert
effects
These
results
suggest
can
CRKP,
potential
mechanisms
including
inhibition
formation,
structure
energy
metabolism.
