Multi-scale optoacoustic molecular imaging of brain diseases

European Journal of Nuclear Medicine and Molecular Imaging, Год журнала: 2021, Номер 48(13), С. 4152 - 4170

Опубликована: Фев. 16, 2021

Abstract The ability to non-invasively visualize endogenous chromophores and exogenous probes sensors across the entire rodent brain with high spatial temporal resolution has empowered optoacoustic imaging modalities unprecedented capacities for interrogating under physiological diseased conditions. This rapidly transformed microscopy (OAM) multi-spectral tomography (MSOT) into emerging research tools study animal models of diseases. In this review, we describe principles showcase recent technical advances that enable high-resolution real-time observations in preclinical models. addition, advanced molecular probe designs allow efficient visualization pathophysiological processes playing a central role variety neurodegenerative diseases, tumors, stroke. We outstanding challenges methodologies propose future outlook.

Язык: Английский

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A Systematic Review of Cerebral Functional Near-Infrared Spectroscopy in Chronic Neurological Diseases—Actual Applications and Future Perspectives

Diagnostics, Год журнала: 2020, Номер 10(8), С. 581 - 581

Опубликована: Авг. 12, 2020

Background: The management of people affected by age-related neurological disorders requires the adoption targeted and cost-effective interventions to cope with chronicity. Therapy adaptation rehabilitation represent major targets requiring long-term follow-up neurodegeneration or, conversely, promotion neuroplasticity mechanisms. However, affordable reliable neurophysiological correlates cerebral activity be used throughout treatment stages are often lacking. aim this systematic review is highlight actual applications functional Near-Infrared Spectroscopy (fNIRS) as a versatile optical neuroimaging technology for investigating cortical hemodynamic in most common chronic conditions. Methods: We reviewed studies fNIRS Parkinson’s Disease (PD), Alzheimer’s (AD) Mild Cognitive Impairment (MCI) those focusing on motor cognitive impairment ageing Multiple Sclerosis (MS) disease young adults. literature search was conducted NCBI PubMed Web Science databases PRISMA guidelines. Results: identified total 63 peer-reviewed articles. AD spectrum investigated pathology 40 articles ranging from traditional monitoring tissue oxygenation analysis resting-state conditions or functions means memory verbal fluency tasks. Conversely, PD (12 articles) MS (11 mainly focused characterization their association dual-task area prefrontal cortex, since reported play an important role compensatory mechanism neurofunctional changes associated these Interestingly, only 9 applied longitudinal approach. Conclusion: results indicate that employed cross-sectional clinical phenotypes pathologies, whereas data its utility surrogate biomarkers progression effects promising but still

Язык: Английский

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Multimodal Noninvasive Functional Neurophotonic Imaging of Murine Brain‐Wide Sensory Responses

Advanced Science, Год журнала: 2022, Номер 9(24)

Опубликована: Июль 7, 2022

Modern optical neuroimaging approaches are expanding the ability to elucidate complex brain function. Diverse imaging contrasts enable direct observation of neural activity with functional sensors along induced hemodynamic responses. To date, decoupling interplay neurovascular coupling and dynamical physiological states has remained challenging when employing single-modality readings. A hybrid fluorescence optoacoustic tomography platform combined a custom data processing pipeline based on statistical parametric mapping is devised, attaining first noninvasive simultaneous calcium activation patterns using contrasts. Correlated changes in oxy- deoxygenated hemoglobin, total oxygen saturation, rapid GCaMP6f signals observed response peripheral sensory stimulation. While concurrent epifluorescence serves corroborate complement observations, latter further aids responses from slowly varying background recordings mediated by changes. The expands capabilities conventional methods provide more comprehensive readings for studying neurometabolic mechanisms related diseases.

Язык: Английский

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Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Frontiers in Neuroscience, Год журнала: 2021, Номер 15

Опубликована: Июнь 10, 2021

A prominent goal of neuroscience is to improve our understanding how brain structure and activity interact produce perception, emotion, behavior, cognition. The brain’s network inherently organized in distinct spatiotemporal patterns that span scales from nanometer-sized synapses meter-long nerve fibers millisecond intervals between electrical signals decades memory storage. There currently no single imaging method alone can provide all the relevant information, but intelligent combinations complementary techniques be effective. Here, we thus present latest advances biomedical biological engineering on photoacoustic neuroimaging context techniques. particular focus placed recent whole-brain rodent models its influential role bridging gap fluorescence microscopy more non-invasive such as magnetic resonance (MRI). We consider current strategies address persistent challenges, particularly developing molecular contrast agents, conclude with an overview potential future directions for deeper insights into healthy pathological processes.

Язык: Английский

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3D Monte Carlo simulation of light distribution in mouse brain in quantitative photoacoustic computed tomography

Quantitative Imaging in Medicine and Surgery, Год журнала: 2020, Номер 11(3), С. 1046 - 1059

Опубликована: Март 1, 2020

Photoacoustic computed tomography (PACT) detects light-induced ultrasound (US) waves to reconstruct the optical absorption contrast of biological tissues. Due its relatively deep penetration (several centimeters in soft tissue), high spatial resolution, and inherent functional sensitivity, PACT has great potential for imaging mouse brains with endogenous exogenous contrasts, which is immense interest neuroscience community. However, conventional either assumes homogenous fluence within brain or uses a simplified attenuation model estimation. Both approaches underestimate complexity heterogeneity can result poor quantitative accuracy.To optimize performance PACT, we explore first time 3D Monte Carlo (MC) simulation study distribution complete model. We apply MCX MC package on digital (Digimouse) atlas that anatomy information. To evaluate impact vasculature light delivery, also incorporate whole-brain Digimouse atlas. k-wave toolbox was used investigate effect inhomogeneous illumination reconstructed images chromophore concentration estimation.The results clearly show heterogeneous at global level decrease by factor five increasing depth. Moreover, strong scattering induce disturbance local level.Both contributes reduced accuracy brain. Correcting improve PACT.

Язык: Английский

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Hemodynamic response to sensory stimulation in mice: Comparison between functional ultrasound and optoacoustic imaging

NeuroImage, Год журнала: 2021, Номер 237, С. 118111 - 118111

Опубликована: Апрель 30, 2021

Intense efforts are underway to develop functional imaging modalities for capturing brain activity at the whole organ scale with high spatial and temporal resolution. Functional optoacoustic (fOA) is emerging as a new tool monitor multiple hemodynamic parameters across mouse brain, but its sound validation against other neuroimaging often lacking. Here we investigate responses peripheral sensory stimulation using both fOA ultrasound (fUS) imaging. The two operate under similar spatio-temporal resolution regime, potential provide synergistic complementary readouts. Specific contralateral activation was observed sub-millimeter methods. Sensitivity found be on comparable levels, strongest obtained in oxygenated hemoglobin channel of fOA. While techniques attained highly correlated responses, differential readings deoxygenated haemoglobin provided information blood flow contrast fUS. multi-modal approach may thus emerge powerful providing insights into function, complementing our current knowledge generated well-established

Язык: Английский

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Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice

Advanced Science, Год журнала: 2022, Номер 10(3)

Опубликована: Ноя. 27, 2022

Abstract Functional magnetic resonance imaging (fMRI) has massively contributed to the understanding of mammalian brain function. However, origin and interpretation blood oxygen level‐dependent (BOLD) signals retrieved by fMRI remain highly disputed. This article reports on development a fully hybridized system enabling concurrent functional optoacoustic tomography (MROT) measurements stimulus‐evoked brain‐wide sensory responses in mice. The complementary angiographic soft tissue contrasts both modalities along with simultaneous multi‐parametric readings hemodynamic are leveraged order establish unequivocal links between various counteracting physiological metabolic processes brain. results indicate that BOLD correlated, spatially temporally, total hemoglobin resolved volumetric multi‐spectral tomography. Furthermore, differential oxygenated deoxygenated exhibit superior sensitivity as compared when detecting responses. MROT approach greatly expands neuroimaging toolset comprehensively study neurovascular neurometabolic coupling mechanisms related diseases.

Язык: Английский

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Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 25, 2024

Abstract Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding function. The existing neuroimaging techniques are largely limited by restricted field view, slow speed, or otherwise do not have the adequate spatial to capture activities on a capillary and cellular level. To address these limitations, we introduce fluorescence localization microscopy aided sparsely-labeled red blood cells cortex-wide morphological functional cerebral angiography 4.9 µm 1 s temporal resolution. When combined calcium imaging, proposed method enables extended recordings stimulus-evoked neuro-vascular changes in murine while providing simultaneous multiparametric readings intracellular neuronal activity, flow velocity/direction/volume, vessel diameter. Owing its simplicity versatility, approach will become an invaluable tool deciphering regulation cortical microcirculation neurovascular coupling health disease.

Язык: Английский

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Deep learning facilitates fully automated brain image registration of optoacoustic tomography and magnetic resonance imaging

Biomedical Optics Express, Год журнала: 2022, Номер 13(9), С. 4817 - 4817

Опубликована: Июль 18, 2022

Multispectral optoacoustic tomography (MSOT) is an emerging optical imaging method providing multiplex molecular and functional information from the rodent brain. It can be greatly augmented by magnetic resonance (MRI) which offers excellent soft-tissue contrast high-resolution brain anatomy. Nevertheless, registration of MSOT-MRI images remains challenging, chiefly due to entirely different image rendered these two modalities. Previously reported algorithms mostly relied on manual user-dependent segmentation, compromised data interpretation quantification. Here we propose a fully automated for multimodal empowered deep learning. The workflow includes neural network-based segmentation generate suitable masks, are subsequently registered using additional network. performance algorithm showcased with datasets acquired cross-sectional MSOT high-field MRI preclinical scanners. further validated half-automated registration, demonstrating its robustness accuracy.

Язык: Английский

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Volumetric registration framework for multimodal functional magnetic resonance and optoacoustic tomography of the rodent brain

Photoacoustics, Год журнала: 2023, Номер 31, С. 100522 - 100522

Опубликована: Июнь 1, 2023

Optoacoustic tomography (OAT) provides a non-invasive means to characterize cerebral hemodynamics across an entire murine brain while attaining multi-parametric readouts not available with other modalities. This unique capability can massively impact our understanding of function. However, OAT largely lacks the soft tissue contrast required for unambiguous identification regions. Hence, its accurate registration reference atlas is paramount meaningful functional readings. Herein, we capitalized on simultaneously acquired bi-modal data from recently-developed hybrid magnetic resonance optoacoustic (MROT) scanner in order devise image coregistration paradigm that facilitates parcellation and anatomical referencing. We evaluated performance proposed methodology by coregistering standalone system using different methods. The enhanced further demonstrated analysis characterization stimulus-evoked responses. suggested approach enables better consolidation research findings thus facilitating wider acceptance as powerful neuroimaging tool study functions diseases.

Язык: Английский

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