bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Ноя. 10, 2021
Abstract
Modern
optical
neuroimaging
approaches
are
expanding
our
ability
to
elucidate
complex
brain
function.
Diverse
imaging
contrasts
enable
direct
observation
of
neural
activity
with
functional
sensors
along
the
induced
hemodynamic
responses.
To
date,
decoupling
interplay
neurovascular
coupling
and
dynamical
physiological
states
has
remained
challenging
when
employing
single-modality
tools.
We
devised
a
hybrid
fluorescence
optoacoustic
tomography
(FLOT)
platform
combined
custom
data
processing
pipeline
based
on
statistical
parametric
mapping,
accomplishing
first
simultaneous
noninvasive
both
indirect
brain-wide
activation
patterns
contrast.
Correlated
changes
in
oxy-
deoxygenated
hemoglobin,
total
oxygen
saturation
rapid
GCaMP6f
signals
were
observed
response
peripheral
sensory
stimulation.
While
concurrent
epifluorescence
served
corroborate
complement
observations,
latter
further
aided
calcium
responses
from
slowly
varying
background
recordings
mediated
by
changes.
The
expands
capabilities
conventional
methods
provide
more
comprehensive
readings
for
studying
neurometabolic
mechanisms
related
diseases.
European Journal of Nuclear Medicine and Molecular Imaging,
Год журнала:
2021,
Номер
48(13), С. 4152 - 4170
Опубликована: Фев. 16, 2021
Abstract
The
ability
to
non-invasively
visualize
endogenous
chromophores
and
exogenous
probes
sensors
across
the
entire
rodent
brain
with
high
spatial
temporal
resolution
has
empowered
optoacoustic
imaging
modalities
unprecedented
capacities
for
interrogating
under
physiological
diseased
conditions.
This
rapidly
transformed
microscopy
(OAM)
multi-spectral
tomography
(MSOT)
into
emerging
research
tools
study
animal
models
of
diseases.
In
this
review,
we
describe
principles
showcase
recent
technical
advances
that
enable
high-resolution
real-time
observations
in
preclinical
models.
addition,
advanced
molecular
probe
designs
allow
efficient
visualization
pathophysiological
processes
playing
a
central
role
variety
neurodegenerative
diseases,
tumors,
stroke.
We
outstanding
challenges
methodologies
propose
future
outlook.
Diagnostics,
Год журнала:
2020,
Номер
10(8), С. 581 - 581
Опубликована: Авг. 12, 2020
Background:
The
management
of
people
affected
by
age-related
neurological
disorders
requires
the
adoption
targeted
and
cost-effective
interventions
to
cope
with
chronicity.
Therapy
adaptation
rehabilitation
represent
major
targets
requiring
long-term
follow-up
neurodegeneration
or,
conversely,
promotion
neuroplasticity
mechanisms.
However,
affordable
reliable
neurophysiological
correlates
cerebral
activity
be
used
throughout
treatment
stages
are
often
lacking.
aim
this
systematic
review
is
highlight
actual
applications
functional
Near-Infrared
Spectroscopy
(fNIRS)
as
a
versatile
optical
neuroimaging
technology
for
investigating
cortical
hemodynamic
in
most
common
chronic
conditions.
Methods:
We
reviewed
studies
fNIRS
Parkinson’s
Disease
(PD),
Alzheimer’s
(AD)
Mild
Cognitive
Impairment
(MCI)
those
focusing
on
motor
cognitive
impairment
ageing
Multiple
Sclerosis
(MS)
disease
young
adults.
literature
search
was
conducted
NCBI
PubMed
Web
Science
databases
PRISMA
guidelines.
Results:
identified
total
63
peer-reviewed
articles.
AD
spectrum
investigated
pathology
40
articles
ranging
from
traditional
monitoring
tissue
oxygenation
analysis
resting-state
conditions
or
functions
means
memory
verbal
fluency
tasks.
Conversely,
PD
(12
articles)
MS
(11
mainly
focused
characterization
their
association
dual-task
area
prefrontal
cortex,
since
reported
play
an
important
role
compensatory
mechanism
neurofunctional
changes
associated
these
Interestingly,
only
9
applied
longitudinal
approach.
Conclusion:
results
indicate
that
employed
cross-sectional
clinical
phenotypes
pathologies,
whereas
data
its
utility
surrogate
biomarkers
progression
effects
promising
but
still
Modern
optical
neuroimaging
approaches
are
expanding
the
ability
to
elucidate
complex
brain
function.
Diverse
imaging
contrasts
enable
direct
observation
of
neural
activity
with
functional
sensors
along
induced
hemodynamic
responses.
To
date,
decoupling
interplay
neurovascular
coupling
and
dynamical
physiological
states
has
remained
challenging
when
employing
single-modality
readings.
A
hybrid
fluorescence
optoacoustic
tomography
platform
combined
a
custom
data
processing
pipeline
based
on
statistical
parametric
mapping
is
devised,
attaining
first
noninvasive
simultaneous
calcium
activation
patterns
using
contrasts.
Correlated
changes
in
oxy-
deoxygenated
hemoglobin,
total
oxygen
saturation,
rapid
GCaMP6f
signals
observed
response
peripheral
sensory
stimulation.
While
concurrent
epifluorescence
serves
corroborate
complement
observations,
latter
further
aids
responses
from
slowly
varying
background
recordings
mediated
by
changes.
The
expands
capabilities
conventional
methods
provide
more
comprehensive
readings
for
studying
neurometabolic
mechanisms
related
diseases.
Frontiers in Neuroscience,
Год журнала:
2021,
Номер
15
Опубликована: Июнь 10, 2021
A
prominent
goal
of
neuroscience
is
to
improve
our
understanding
how
brain
structure
and
activity
interact
produce
perception,
emotion,
behavior,
cognition.
The
brain’s
network
inherently
organized
in
distinct
spatiotemporal
patterns
that
span
scales
from
nanometer-sized
synapses
meter-long
nerve
fibers
millisecond
intervals
between
electrical
signals
decades
memory
storage.
There
currently
no
single
imaging
method
alone
can
provide
all
the
relevant
information,
but
intelligent
combinations
complementary
techniques
be
effective.
Here,
we
thus
present
latest
advances
biomedical
biological
engineering
on
photoacoustic
neuroimaging
context
techniques.
particular
focus
placed
recent
whole-brain
rodent
models
its
influential
role
bridging
gap
fluorescence
microscopy
more
non-invasive
such
as
magnetic
resonance
(MRI).
We
consider
current
strategies
address
persistent
challenges,
particularly
developing
molecular
contrast
agents,
conclude
with
an
overview
potential
future
directions
for
deeper
insights
into
healthy
pathological
processes.
Quantitative Imaging in Medicine and Surgery,
Год журнала:
2020,
Номер
11(3), С. 1046 - 1059
Опубликована: Март 1, 2020
Photoacoustic
computed
tomography
(PACT)
detects
light-induced
ultrasound
(US)
waves
to
reconstruct
the
optical
absorption
contrast
of
biological
tissues.
Due
its
relatively
deep
penetration
(several
centimeters
in
soft
tissue),
high
spatial
resolution,
and
inherent
functional
sensitivity,
PACT
has
great
potential
for
imaging
mouse
brains
with
endogenous
exogenous
contrasts,
which
is
immense
interest
neuroscience
community.
However,
conventional
either
assumes
homogenous
fluence
within
brain
or
uses
a
simplified
attenuation
model
estimation.
Both
approaches
underestimate
complexity
heterogeneity
can
result
poor
quantitative
accuracy.To
optimize
performance
PACT,
we
explore
first
time
3D
Monte
Carlo
(MC)
simulation
study
distribution
complete
model.
We
apply
MCX
MC
package
on
digital
(Digimouse)
atlas
that
anatomy
information.
To
evaluate
impact
vasculature
light
delivery,
also
incorporate
whole-brain
Digimouse
atlas.
k-wave
toolbox
was
used
investigate
effect
inhomogeneous
illumination
reconstructed
images
chromophore
concentration
estimation.The
results
clearly
show
heterogeneous
at
global
level
decrease
by
factor
five
increasing
depth.
Moreover,
strong
scattering
induce
disturbance
local
level.Both
contributes
reduced
accuracy
brain.
Correcting
improve
PACT.
NeuroImage,
Год журнала:
2021,
Номер
237, С. 118111 - 118111
Опубликована: Апрель 30, 2021
Intense
efforts
are
underway
to
develop
functional
imaging
modalities
for
capturing
brain
activity
at
the
whole
organ
scale
with
high
spatial
and
temporal
resolution.
Functional
optoacoustic
(fOA)
is
emerging
as
a
new
tool
monitor
multiple
hemodynamic
parameters
across
mouse
brain,
but
its
sound
validation
against
other
neuroimaging
often
lacking.
Here
we
investigate
responses
peripheral
sensory
stimulation
using
both
fOA
ultrasound
(fUS)
imaging.
The
two
operate
under
similar
spatio-temporal
resolution
regime,
potential
provide
synergistic
complementary
readouts.
Specific
contralateral
activation
was
observed
sub-millimeter
methods.
Sensitivity
found
be
on
comparable
levels,
strongest
obtained
in
oxygenated
hemoglobin
channel
of
fOA.
While
techniques
attained
highly
correlated
responses,
differential
readings
deoxygenated
haemoglobin
provided
information
blood
flow
contrast
fUS.
multi-modal
approach
may
thus
emerge
powerful
providing
insights
into
function,
complementing
our
current
knowledge
generated
well-established
Abstract
Functional
magnetic
resonance
imaging
(fMRI)
has
massively
contributed
to
the
understanding
of
mammalian
brain
function.
However,
origin
and
interpretation
blood
oxygen
level‐dependent
(BOLD)
signals
retrieved
by
fMRI
remain
highly
disputed.
This
article
reports
on
development
a
fully
hybridized
system
enabling
concurrent
functional
optoacoustic
tomography
(MROT)
measurements
stimulus‐evoked
brain‐wide
sensory
responses
in
mice.
The
complementary
angiographic
soft
tissue
contrasts
both
modalities
along
with
simultaneous
multi‐parametric
readings
hemodynamic
are
leveraged
order
establish
unequivocal
links
between
various
counteracting
physiological
metabolic
processes
brain.
results
indicate
that
BOLD
correlated,
spatially
temporally,
total
hemoglobin
resolved
volumetric
multi‐spectral
tomography.
Furthermore,
differential
oxygenated
deoxygenated
exhibit
superior
sensitivity
as
compared
when
detecting
responses.
MROT
approach
greatly
expands
neuroimaging
toolset
comprehensively
study
neurovascular
neurometabolic
coupling
mechanisms
related
diseases.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 25, 2024
Abstract
Large-scale
imaging
of
brain
activity
with
high
spatio-temporal
resolution
is
crucial
for
advancing
our
understanding
function.
The
existing
neuroimaging
techniques
are
largely
limited
by
restricted
field
view,
slow
speed,
or
otherwise
do
not
have
the
adequate
spatial
to
capture
activities
on
a
capillary
and
cellular
level.
To
address
these
limitations,
we
introduce
fluorescence
localization
microscopy
aided
sparsely-labeled
red
blood
cells
cortex-wide
morphological
functional
cerebral
angiography
4.9
µm
1
s
temporal
resolution.
When
combined
calcium
imaging,
proposed
method
enables
extended
recordings
stimulus-evoked
neuro-vascular
changes
in
murine
while
providing
simultaneous
multiparametric
readings
intracellular
neuronal
activity,
flow
velocity/direction/volume,
vessel
diameter.
Owing
its
simplicity
versatility,
approach
will
become
an
invaluable
tool
deciphering
regulation
cortical
microcirculation
neurovascular
coupling
health
disease.
Biomedical Optics Express,
Год журнала:
2022,
Номер
13(9), С. 4817 - 4817
Опубликована: Июль 18, 2022
Multispectral
optoacoustic
tomography
(MSOT)
is
an
emerging
optical
imaging
method
providing
multiplex
molecular
and
functional
information
from
the
rodent
brain.
It
can
be
greatly
augmented
by
magnetic
resonance
(MRI)
which
offers
excellent
soft-tissue
contrast
high-resolution
brain
anatomy.
Nevertheless,
registration
of
MSOT-MRI
images
remains
challenging,
chiefly
due
to
entirely
different
image
rendered
these
two
modalities.
Previously
reported
algorithms
mostly
relied
on
manual
user-dependent
segmentation,
compromised
data
interpretation
quantification.
Here
we
propose
a
fully
automated
for
multimodal
empowered
deep
learning.
The
workflow
includes
neural
network-based
segmentation
generate
suitable
masks,
are
subsequently
registered
using
additional
network.
performance
algorithm
showcased
with
datasets
acquired
cross-sectional
MSOT
high-field
MRI
preclinical
scanners.
further
validated
half-automated
registration,
demonstrating
its
robustness
accuracy.
Photoacoustics,
Год журнала:
2023,
Номер
31, С. 100522 - 100522
Опубликована: Июнь 1, 2023
Optoacoustic
tomography
(OAT)
provides
a
non-invasive
means
to
characterize
cerebral
hemodynamics
across
an
entire
murine
brain
while
attaining
multi-parametric
readouts
not
available
with
other
modalities.
This
unique
capability
can
massively
impact
our
understanding
of
function.
However,
OAT
largely
lacks
the
soft
tissue
contrast
required
for
unambiguous
identification
regions.
Hence,
its
accurate
registration
reference
atlas
is
paramount
meaningful
functional
readings.
Herein,
we
capitalized
on
simultaneously
acquired
bi-modal
data
from
recently-developed
hybrid
magnetic
resonance
optoacoustic
(MROT)
scanner
in
order
devise
image
coregistration
paradigm
that
facilitates
parcellation
and
anatomical
referencing.
We
evaluated
performance
proposed
methodology
by
coregistering
standalone
system
using
different
methods.
The
enhanced
further
demonstrated
analysis
characterization
stimulus-evoked
responses.
suggested
approach
enables
better
consolidation
research
findings
thus
facilitating
wider
acceptance
as
powerful
neuroimaging
tool
study
functions
diseases.