Ulvophyte Green Algae Caulerpa lentillifera: Metabolites Profile and Antioxidant, Anticancer, Anti-Obesity, and In Vitro Cytotoxicity Properties

Molecules, Год журнала: 2023, Номер 28(3), С. 1365 - 1365

Опубликована: Янв. 31, 2023

Marine algae have excellent bioresource properties with potential nutritional and bioactive therapeutic benefits, but studies regarding Caulerpa lentillifera are limited. This study aims to explore the metabolites profile antioxidant, anticancer, anti-obesity, in vitro cytotoxicity of fractionated ethanolic extract C. using two maceration soxhlet extraction methods. Dried simplicia was mashed extracted ethanol solvent, concentrated evaporated, then sequentially partitioned equal volumes ethyl acetate n-Hexane. Six samples were used this study, consisting ME (Maceration-Ethanol), MEA (Maceration-Ethyl Acetate), MH (Maceration-n-Hexane), SE (Soxhletation-Ethanol), SEA (Soxhletation-Ethyl SH (Soxhletation-n-Hexane). Non-targeted metabolomic profiling determined LC-HRMS, while anticancer DPPH ABTS, lipase inhibition, MTT assay, respectively. demonstrates that has several functional metabolites, antioxidant capacity (EC50 is very close EC50 Trolox), as well anti-obesity < orlistat, an inhibitor lipid hydrolyzing enzymes), which useful precursors for new approaches improving obesity-related diseases. More interestingly, ME, MH, novel agents drugs, especially hepatoma, breast, colorectal, leukemia cancers. Finally, can be a nutraceutical great benefits.

Язык: Английский

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Dietary Supplementation of Caulerpa racemosa Ameliorates Cardiometabolic Syndrome via Regulation of PRMT-1/DDAH/ADMA Pathway and Gut Microbiome in Mice

Nutrients, Год журнала: 2023, Номер 15(4), С. 909 - 909

Опубликована: Фев. 11, 2023

This study evaluated the effects of an aqueous extract Caulerpa racemosa (AEC) on cardiometabolic syndrome markers, and modulation gut microbiome in mice administered a cholesterol- fat-enriched diet (CFED). Four groups received different treatments: normal diet, CFED, CFED added with AEC at 65 130 mg/kg body weight (BW). The effective concentration (EC50) values for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), lipase inhibition were lower than those controls vitro. In model, administration high-dose showed improved lipid blood glucose profiles reduction endothelial dysfunction markers (PRMT-1 ADMA). Furthermore, correlation between specific microbiomes biomarkers associated diseases was also observed. vitro studies highlighted antioxidant properties AEC, while vivo data demonstrated that plays role management via regulation oxidative stress, inflammation, function (PRMT-1/DDAH/ADMA pathway), microbiota.

Язык: Английский

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Phytochemical analysis, antioxidant activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells

BMC Complementary Medicine and Therapies, Год журнала: 2025, Номер 25(1)

Опубликована: Март 11, 2025

Marine algae have excellent phytoconstituents with notable biological activity and bioactive therapeutic benefits, but the anti-oral cancer of Caulerpa lentillifera (C. lentillifera) has not been widely studied. This study aimed to explore anti-cancer properties C. gain insights into possible treatment approaches. The three extracts were prepared using maceration method methanol (CLM), ethanol (CLE), acetone (CLA). chemical composition was investigated. Its metabolite profiles selectively further investigated LC-QTOF MS/MS technique their antioxidative evaluated. cytotoxic effect on KON cells MRC-5 assessed MTT test. Morphological changes apoptosis examined through Hoechst 33,258 AO double staining, while DAPI FDA labeling used observe nucleus cytoplasm. Using a flow cytometer, percentage cell cycle arrest calculated fraction death examined. CLA exhibited higher quantities TPC, TFC, chlorophyll a, b compared CLM CLE. analysis revealed ten major phytochemicals in CLA. activity, CLE demonstrating significantly antioxidant In-vitro, oral sensitivity CLA, CLE, that order. induced ROS-mediated as well disruption mitochondrial membrane potential, concentrations at IC40, IC60, IC80 leading within 24 h. Furthermore, blocked sub-G G0-G1 by all extracts. Notably, impeded colony growth, migration, invasion. increase cellular uptake measured TEER findings showed several functional metabolites, strong anti-tumor properties. According these results, may be utilized treat cancer.

Язык: Английский

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In silico and in vitro studies reveal a synergistic potential source of novel anti-ageing from two Indonesian green algae

Journal of Functional Foods, Год журнала: 2023, Номер 104, С. 105555 - 105555

Опубликована: Апрель 24, 2023

The exploration of natural ingredients to prevent or delay ageing is fascinating develop in the industrial technologies nutraceuticals. Indonesian green algae (Caulerpa racemosa and Caulerpa lentillifera) are predicted contain many bioactive substances that have not been studied intensively but potential be a source anti-ageing. Therefore, this study examine content with related proteins play an important role process vitro study. This reveals novel anti-ageing from two C. lentillifera via modulation mTOR-SIRT1-AMPK pathways their antioxidant abilities. Based on integration these silico vitro, candidates for nutraceuticals pharmaceutical agents, which supported by synergistic effect capacity against free radicals oxidative stress.

Язык: Английский

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Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation

Journal of Agriculture and Food Research, Год журнала: 2023, Номер 12, С. 100621 - 100621

Опубликована: Май 13, 2023

Marine and terrestrial organisms are rich in chemical compounds with medicinal pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is potential source of novel therapeutic human cancer. This study aimed to determine the anticancer activity C. racemosa extracts breast cancer cells, identify compounds, mechanism using computational models. Seaweed (C. racemosa) was taken from North Sulawesi, Indonesia; Followed by authentication identification according previously published protocol extracted three different solvent: hexane, ethyl acetate, ethanol. were evaluated cytotoxicity against MCF-7 cells MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay. Antioxidant activities assessed based on free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH)) ferric-reducing antioxidant power (FRAP) assays. The phytochemical constituents identified liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) system. interaction epidermal growth factor 2 (HER2) protein achieved molecular docking PyRx-vina application protein-ligand complex visualization. hexane extract exhibited highest followed acetate ethanol (IC50 23.7 ± 2.0; 66.7 5.8 182.7 14.3 μg/mL). best sample DPPH 21.5 2.0 μg/mL) while most active FRAP value (14.5 1.3 μg gallic acid equivalent/g). Data LC-ESI-QTOF-MS allowed 21 compounds. showed that 12 could prevent tumors acting as inhibitors anti-apoptotic HER2 protein. has chemoprevention through its capacity inhibition More studies needed investigate efficacy

Язык: Английский

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Green seaweeds fatty acids and heterocyclic derivatives against cancer: Opinion on future nutraceutical application

Frontiers in Oncology, Год журнала: 2023, Номер 13

Опубликована: Фев. 14, 2023

OPINION article Front. Oncol., 14 February 2023Sec. Molecular and Cellular Oncology Volume 13 - 2023 | https://doi.org/10.3389/fonc.2023.1145919

Язык: Английский

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Physicochemical characterization of novel toothpaste from Caulerpa racemosa and Thunnus fish bone: Antibacterial potency against colonization of selected cariogenic-periodontal bacteria

Pharmacia, Год журнала: 2024, Номер 71, С. 1 - 8

Опубликована: Янв. 24, 2024

This study evaluated the physicochemical properties of toothpaste from combined Caulerpa racemosa and Thunnus fish bone (Toothpaste or TCT) its antibacterial activity towards colonization selected cariogenic periodontal bacterias. Four forms which contained C. extract calcium carbonate isolates tuna control (F1 (1.5:45); F2 (3:45); F3 (4.5:45); F4 (0:45)) were compared analyzed for antioxidant (DPPH assay), organoleptic (sensory), homogeneity, viscosity, pH, foamability. Antibacterial tests conducted on Streptococcus mutans , Staphylococcus aureus Porphyromonas gingivalis . The group’s (F1, F2, F3, F4) p=0.0001 differed considerably (CI 95%). was most antioxidant-active formula, with 27.46 ± 3.09%. also had good sensory tests, adequate optimal pH 7.64 0.68, an increased viscosity level 443.07 0.12, least foam formations 19.28 0.07, all are significantly different (p&lt;0.05) other variations TCT formulas. Interestingly, has greater inhibition against In conclusion, formula 3 (F3) is a recommended toothpaste, made bone, promising properties. A further clinical urgently needed.

Язык: Английский

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Unleashing the Potential of Marine Algae in Cancer Prevention and Treatment Through Combination of Tradition and Innovation

Interdisciplinary cancer research, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Cancer is a global health challenge, with rapidly rising incidence and significant economic burden. The urgent need for innovative approaches to cancer prevention treatment underscored by its growing impact. Marine algae, characterized their unique bioactive compounds, have emerged as promising sources of novel anticancer agents in this context. This chapter reviews the potential marine algae treatment, emphasizing integration traditional knowledge modern scientific methods develop safe, affordable, accessible drugs. offer rich source including polysaccharides, polyphenols, pigments, known antioxidant, anti-inflammatory, antitumor effects. Moreover, these organisms evolved complex defense mechanisms, making them particularly interesting drug discovery. Research highlights multifaceted mechanisms apoptosis induction, cell cycle arrest, selective cytotoxicity against cells. Fucoxanthin, fucoidan, stigmasterol, other compounds from shown potent properties through modulation various signaling pathways. advantages include chemical diversity, sustainability, environmentally friendly cultivation, presenting exciting opportunities development. To address challenges herbal medicine, standardization, active compound identification, rigorous clinical trials are essential. Collaboration between practitioners scientists can bridge gap wisdom medical standards. Combinatorial therapies that integrate extracts conventional treatments more effective holistic management.

Язык: Английский

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Sea grapes (Caulerpa lentillifera J. Agardh, Chlorophyta) for human use: Structured review on recent research in cultivation, nutritional value, and post-harvest management

Journal of Applied Phycology, Год журнала: 2023, Номер 35(6), С. 2957 - 2983

Опубликована: Июль 27, 2023

Abstract Seaweeds are a major contributor to global marine aquaculture production, with the biomass being mainly used, among others, for human nutrition, pharmaceutics, and cosmetics. However, green seaweeds severely underrepresented, compared red brown macroalgae. Caulerpa lentillifera (known as “sea grapes” or “green caviar”) is an edible, seaweed distinctive texture various nutritional benefits. In this review, all articles on sea grapes published between 1900 October 2022 found in scientific citation databases Scopus Web of Science (search string: “caulerpa” AND “lentillifera”) were grouped by research topic intended application following PRISMA approach. 51% 130 included review focused “Biochemical composition”, followed “Water treatment” (18%) “Ecophysiology” (15%). The most prominent was “Pharmaceutics”, “Cultivation” “Fundamental research”. order provide knowledge base researchers practitioners C. aquaculture, that simultaneously under one topics treatment”, applications “Cultivation”, “Nutritional value” “Post-harvest” summarized more detail. Light management grapes, their use high-value co-culture species capacity bioremediate nutrients, well short shelf-life identified important areas interest. assessment revealed several gaps, example need intra-species comparisons biochemical composition across spatial temporal scales.

Язык: Английский

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Dietary supplementation of Muntingia calabura leaves ameliorates reactive oxygen species and malondialdehyde levels: clinical study on alloxan-induced hyperglycemic rats

Clinical Nutrition Open Science, Год журнала: 2023, Номер 48, С. 87 - 96

Опубликована: Март 25, 2023

Язык: Английский

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