Carotenoids Composition of Green Algae Caulerpa racemosa and Their Antidiabetic, Anti-Obesity, Antioxidant, and Anti-Inflammatory Properties

Molecules, Год журнала: 2023, Номер 28(7), С. 3267 - 3267

Опубликована: Апрель 6, 2023

Green alga Caulerpa racemosa is an underexploited species of macroalgae, even though it characterized by a green color that indicates abundance bioactive pigments, such as chlorophyll and possibly xanthophyll. Unlike chlorophyll, which has been well explored, the composition carotenoids C. its biological activities have not reported. Therefore, this study aims to look at carotenoid profile racemose determine their activities, include antidiabetic, anti-obesity, anti-oxidative, anti-inflammatory, cytotoxicity in vitro. The detected were all xanthophylls, included fucoxanthin, lutein, astaxanthin, canthaxanthin, zeaxanthin, β-carotene, β-cryptoxanthin based on orbitrap-mass spectrometry (MS) rapid ultra-high performance liquid chromatography (UHPLC) diode array detector. Of seven observed, should be highlighted β-carotene canthaxanthin two most dominant present racemosa. Interestingly, extract good activity inhibiting α-glucosidase, α-amylase, DPPH ABTS, TNF-α mTOR, upregulating AMPK, makes drug candidate or functional antidiabetic food, very promising anti-obesity anti-inflammatory. More interestingly, value shows level safety normal cells, potential for further development nutraceuticals pharmaceuticals.

Язык: Английский

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Seaweed-derived phenolic compounds as diverse bioactive molecules: A review on identification, application, extraction and purification strategies

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 266, С. 131147 - 131147

Опубликована: Март 26, 2024

Язык: Английский

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Dietary Supplementation of Caulerpa racemosa Ameliorates Cardiometabolic Syndrome via Regulation of PRMT-1/DDAH/ADMA Pathway and Gut Microbiome in Mice

Nutrients, Год журнала: 2023, Номер 15(4), С. 909 - 909

Опубликована: Фев. 11, 2023

This study evaluated the effects of an aqueous extract Caulerpa racemosa (AEC) on cardiometabolic syndrome markers, and modulation gut microbiome in mice administered a cholesterol- fat-enriched diet (CFED). Four groups received different treatments: normal diet, CFED, CFED added with AEC at 65 130 mg/kg body weight (BW). The effective concentration (EC50) values for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), lipase inhibition were lower than those controls vitro. In model, administration high-dose showed improved lipid blood glucose profiles reduction endothelial dysfunction markers (PRMT-1 ADMA). Furthermore, correlation between specific microbiomes biomarkers associated diseases was also observed. vitro studies highlighted antioxidant properties AEC, while vivo data demonstrated that plays role management via regulation oxidative stress, inflammation, function (PRMT-1/DDAH/ADMA pathway), microbiota.

Язык: Английский

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Unlocking the Therapeutic Potential of Algae-Derived Compounds in Hematological Malignancies

Cancers, Год журнала: 2025, Номер 17(2), С. 318 - 318

Опубликована: Янв. 20, 2025

Algae are a rich source of bioactive compounds that have wide range beneficial effects on human health and can show significant potential in the treatment hematological malignancies such as leukemia, lymphoma, multiple myeloma. These diseases often pose therapeutic challenge despite recent advances (e.g., use immunomodulatory drugs, proteasome inhibitors, CD38 monoclonal antibodies, stem cell transplant, targeted therapy). A considerable number patients experience relapses or resistance to applied therapies. Algal compounds, alone combination with chemotherapy other more advanced therapies, exhibited antitumor preclinical studies may improve disease outcomes. include ability induce apoptosis, inhibit tumor growth, immune responses. However, most these conducted vitro, without vivo validation clinical trials. This paper summarizes current evidence vitro algae extracts isolated myeloma lines. In addition, we address application algae-derived drug carriers their synergistic against hematologic malignancies.

Язык: Английский

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Phytochemical analysis, antioxidant activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells

BMC Complementary Medicine and Therapies, Год журнала: 2025, Номер 25(1)

Опубликована: Март 11, 2025

Marine algae have excellent phytoconstituents with notable biological activity and bioactive therapeutic benefits, but the anti-oral cancer of Caulerpa lentillifera (C. lentillifera) has not been widely studied. This study aimed to explore anti-cancer properties C. gain insights into possible treatment approaches. The three extracts were prepared using maceration method methanol (CLM), ethanol (CLE), acetone (CLA). chemical composition was investigated. Its metabolite profiles selectively further investigated LC-QTOF MS/MS technique their antioxidative evaluated. cytotoxic effect on KON cells MRC-5 assessed MTT test. Morphological changes apoptosis examined through Hoechst 33,258 AO double staining, while DAPI FDA labeling used observe nucleus cytoplasm. Using a flow cytometer, percentage cell cycle arrest calculated fraction death examined. CLA exhibited higher quantities TPC, TFC, chlorophyll a, b compared CLM CLE. analysis revealed ten major phytochemicals in CLA. activity, CLE demonstrating significantly antioxidant In-vitro, oral sensitivity CLA, CLE, that order. induced ROS-mediated as well disruption mitochondrial membrane potential, concentrations at IC40, IC60, IC80 leading within 24 h. Furthermore, blocked sub-G G0-G1 by all extracts. Notably, impeded colony growth, migration, invasion. increase cellular uptake measured TEER findings showed several functional metabolites, strong anti-tumor properties. According these results, may be utilized treat cancer.

Язык: Английский

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In silico and in vitro studies reveal a synergistic potential source of novel anti-ageing from two Indonesian green algae

Journal of Functional Foods, Год журнала: 2023, Номер 104, С. 105555 - 105555

Опубликована: Апрель 24, 2023

The exploration of natural ingredients to prevent or delay ageing is fascinating develop in the industrial technologies nutraceuticals. Indonesian green algae (Caulerpa racemosa and Caulerpa lentillifera) are predicted contain many bioactive substances that have not been studied intensively but potential be a source anti-ageing. Therefore, this study examine content with related proteins play an important role process vitro study. This reveals novel anti-ageing from two C. lentillifera via modulation mTOR-SIRT1-AMPK pathways their antioxidant abilities. Based on integration these silico vitro, candidates for nutraceuticals pharmaceutical agents, which supported by synergistic effect capacity against free radicals oxidative stress.

Язык: Английский

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Marine algae‐derived characterized bioactive compounds as therapy for cancer: A review on their classification, mechanism of action, and future perspectives

Phytotherapy Research, Год журнала: 2024, Номер 38(8), С. 4053 - 4080

Опубликована: Июнь 19, 2024

Abstract In 2022, there were around 20 million new cases and over 9.7 cancer‐related deaths worldwide. An increasing number of metabolites with anticancer activity in algae had been isolated identified, which promising candidates for cancer therapy. Red are well‐known the production brominated metabolites, including terpenoids phenols, have capacity to induce cell toxicity. Some non‐toxic biological macromolecules, polysaccharides, distinct secondary found many algae, particularly green algae. They possess activities by inhibiting tumor angiogenesis, stimulating immune response, inducing apoptosis. However, structure–activity relationship between these components antitumor activity, as well certain taxa within remains relatively unstudied. This work is based on reports published from 2003 2024 PubMed ISI Web Science databases. A comprehensive review characterized algal active compounds, together their structure mechanism action was performed. Also, preliminarily summarized better assess potential properties a natural, safe bioactive product be used an alternative treatment cancers, leading opportunities drug discovery.

Язык: Английский

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New Insight on In Vitro Biological Activities of Sulfated Polysaccharides from Ulvophyte Green Algae

Molecules, Год журнала: 2023, Номер 28(11), С. 4531 - 4531

Опубликована: Июнь 2, 2023

Green algae are natural bioresources that have excellent bioactive potential, partly due to sulfated polysaccharides (SPs) which still rarely explored for their biological activities. There is currently an urgent need studies exploring the anticancer activity of SPs extracted from two Indonesian ulvophyte green algae: polysaccharide Caulerpa racemosa (SPCr) and lentillifera (SPCl). The method isolating assessment activities in this study were based on previous similar studies. highest yield sulfate/total sugar ratio was presented by SPCr than SPCl. Overall, exhibits a strong antioxidant activity, as indicated smaller EC50 values obtained series assays compared Trolox (control). As anti-obesity antidiabetic, overall value both close positive control (orlistat acarbose). Even more interesting SPCl displayed wide-ranging effects colorectal, hepatoma, breast cancer cell lines, leukemia. Finally, reveals new insights potential be promising nutraceuticals novel antioxidative actors, able fight obesity, diabetes, even cancer.

Язык: Английский

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Green seaweed Caulerpa racemosa - Chemical constituents, cytotoxicity in breast cancer cells and molecular docking simulation

Journal of Agriculture and Food Research, Год журнала: 2023, Номер 12, С. 100621 - 100621

Опубликована: Май 13, 2023

Marine and terrestrial organisms are rich in chemical compounds with medicinal pharmacological properties, including antitumor agents for chemoprevention. Caulerpa racemosa, a marine species, is potential source of novel therapeutic human cancer. This study aimed to determine the anticancer activity C. racemosa extracts breast cancer cells, identify compounds, mechanism using computational models. Seaweed (C. racemosa) was taken from North Sulawesi, Indonesia; Followed by authentication identification according previously published protocol extracted three different solvent: hexane, ethyl acetate, ethanol. were evaluated cytotoxicity against MCF-7 cells MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay. Antioxidant activities assessed based on free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH)) ferric-reducing antioxidant power (FRAP) assays. The phytochemical constituents identified liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) system. interaction epidermal growth factor 2 (HER2) protein achieved molecular docking PyRx-vina application protein-ligand complex visualization. hexane extract exhibited highest followed acetate ethanol (IC50 23.7 ± 2.0; 66.7 5.8 182.7 14.3 μg/mL). best sample DPPH 21.5 2.0 μg/mL) while most active FRAP value (14.5 1.3 μg gallic acid equivalent/g). Data LC-ESI-QTOF-MS allowed 21 compounds. showed that 12 could prevent tumors acting as inhibitors anti-apoptotic HER2 protein. has chemoprevention through its capacity inhibition More studies needed investigate efficacy

Язык: Английский

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Revealing Novel Source of Breast Cancer Inhibitors from Seagrass Enhalus acoroides: In Silico and In Vitro Studies

Molecules, Год журнала: 2024, Номер 29(5), С. 1082 - 1082

Опубликована: Фев. 29, 2024

Enhalus arcoides is a highly beneficial type of seagrass. Prior studies have presented proof the bioactivity E. acoroides, suggesting its potential to combat cancer. Therefore, this study aims delve deeper into acoroides bioactive molecule profiles and their direct biological anticancer activities potentials through combination in-silico in-vitro studies. This conducted metabolite profile analysis on utilizing HPLC-ESI-HRMS/MS analysis. Two extraction techniques, ethanol hexane, were employed for process. Furthermore, was using molecular docking simulations HER2, EGFR tyrosine kinase HIF-1α protein receptor. Afterward, antioxidant activity metabolites examined ABTS, antiproliferative tested an MTT assay. An revealed ability breast cancer by inhibiting HER2/EGFR/HIF-1α pathway docking. In addition, assay demonstrated that higher dosages from increased effectiveness toxicity against cell lines. Additionally, possess function as potent antioxidants, effectively series carcinogenic mechanisms. Ultimately, showed new approach unveiling metabolites’ receptors, with great cytotoxicity property prevent cascade.

Язык: Английский

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