Abstract
Exosomes,
small
extracellular
vesicles
with
lipid
bilayer
membranes,
play
a
crucial
role
in
cellular
communication
and
can
transfer
diverse
biological
cargo,
including
proteins,
lipids,
nucleic
acids,
from
donor
to
recipient
cells.
Exosomes
possess
immunological
properties,
such
as
antigen
delivery
immune
activation,
along
excellent
drug
capabilities,
making
them
promising
candidates
for
vaccine
development.
For
different
diseases,
exosome‐based
vaccines
be
designed
therapeutic
or
prophylactic
by
leveraging
immunity
humoral
immunity.
With
the
emergence
of
precision
medicine,
personalized
demonstrate
exceptional
potential.
This
review
systematically
introduces
sources,
biogenesis
mechanisms,
components
exosomes
describes
their
regulatory
roles
system.
Subsequently,
preparation,
administration,
therapy
are
discussed.
Finally,
applications
clinical
trials
fields
anti‐infection
anti‐tumor
therapies
particularly
highlighted,
an
analysis
potential
challenges
future
Journal of Hematology & Oncology,
Год журнала:
2023,
Номер
16(1)
Опубликована: Июнь 26, 2023
Abstract
Exosomal
circRNA
serves
a
novel
genetic
information
molecule,
facilitating
communication
between
tumor
cells
and
microenvironmental
cells,
such
as
immune
fibroblasts,
other
components,
thereby
regulating
critical
aspects
of
cancer
progression
including
escape,
angiogenesis,
metabolism,
drug
resistance,
proliferation
metastasis.
Interestingly,
microenvironment
have
new
findings
in
influencing
escape
mediated
by
the
release
exosomal
circRNA.
Given
intrinsic
stability,
abundance,
broad
distribution
circRNAs,
they
represent
excellent
diagnostic
prognostic
biomarkers
for
liquid
biopsy.
Moreover,
artificially
synthesized
circRNAs
may
open
up
possibilities
therapy,
potentially
bolstered
nanoparticles
or
plant
exosome
delivery
strategies.
In
this
review,
we
summarize
functions
underlying
mechanisms
cell
non-tumor
cell-derived
progression,
with
special
focus
on
their
roles
immunity
metabolism.
Finally,
examine
potential
application
therapeutic
targets,
highlighting
promise
clinical
use.
Pharmacological Research,
Год журнала:
2023,
Номер
198, С. 106999 - 106999
Опубликована: Ноя. 19, 2023
Cell-to-cell
communication
strategies
include
extracellular
vesicles
(EVs)
in
plants
and
animals.
The
bioactive
molecules
a
diet
rich
vegetables
fruits
are
associated
with
disease-preventive
effects.
Plant-derived
EVs
(PDEVs)
biogenetically
morphologically
comparable
to
mammalian
transport
molecules,
including
miRNAs.
However,
the
biological
functions
of
PDEVs
not
fully
understood,
standard
isolation
protocols
lacking.
Here,
were
isolated
from
four
foods
combination
ultracentrifugation
size
exclusion
chromatography,
evaluated
as
vehicles
for
enhanced
synthetic
In
addition,
role
food-derived
carriers
dietary
(poly)phenols
other
secondary
metabolites
was
investigated.
broccoli,
pomegranate,
apple,
orange
efficiently
characterized.
all
sources,
4
miRNA
families
present
tissues
EVs.
miRNAs
broccoli
fruit-derived
showed
reduced
RNase
degradation
ferried
inside
exposed
cells.
transfected
ath-miR159a,
ath-miR162a-3p,
ath-miR166b-3p,
ath-miR396b-5p
toxic
effects
on
human
cells,
did
natural
alone.
trace
amounts
phytochemicals,
flavonoids,
anthocyanidins,
phenolic
acids,
or
glucosinolates.
Thus,
can
act
nanocarriers
functional
that
could
be
used
RNA-based
therapy.
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
174, С. 116543 - 116543
Опубликована: Апрель 11, 2024
In
recent
years,
there
has
been
an
increasing
number
of
related
studies
on
exosomes.
Most
have
focused
exosomes
derived
from
mammals,
confirming
the
important
role
that
play
in
cell
communication.
Plants,
as
a
natural
ingredient,
plant-derived
confirmed
to
similar
structures
and
functions
mammalian-derived
Plant-derived
exosome-like
nanoparticles
(PELNs)
are
lipid
bilayer
membrane
nanovesicles
containing
bioactive
constituents
such
miRNA,
mRNA,
protein,
lipids
obtained
plant
cells,
can
participate
intercellular
communication
mediate
transboundary
communication,
high
bioavailability
low
immunogenicity,
relatively
safe,
shown
maintaining
homeostasis
preventing,
treating
variety
diseases.
this
review,
we
describe
biogenesis,
isolation
purification
methods,
structural
composition,
stability,
safety,
function
PELNs
challenges.
The
anti-inflammatory,
antioxidant,
antitumor
drug
delivery
mainly
described,
status
research
exosome
Chinese
herbal
medicines
is
outlined.
Overall,
summarized
importance
latest
results
field
provided
theoretical
basis
for
future
clinical
application
PELNs.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 1189 - 1204
Опубликована: Фев. 1, 2024
Abstract:
Tissue
engineering
holds
great
potential
for
tissue
repair
and
rejuvenation.
Plant-derived
exosome-like
nanoparticles
(ELNs)
have
recently
emerged
as
a
promising
avenue
in
engineering.
However,
there
is
an
urgent
need
to
understand
how
plant
ELNs
can
be
therapeutically
applied
clinical
disease
management,
especially
regeneration.
In
this
review,
we
comprehensively
examine
the
properties,
characteristics,
isolation
techniques
of
ELNs.
We
also
discuss
their
impact
on
immune
system,
compatibility
with
human
body,
role
To
ensure
suitability
engineering,
explore
various
modification
strategies.
Additionally,
provide
insights
into
progress
commercialization
industrial
perspectives
This
review
aims
highlight
regenerative
medicine
by
exploring
current
research
landscape
key
findings.
Keywords:
plant,
nanoparticles,
nanomedicine,
cartilage,
wound,
bone
Theranostics,
Год журнала:
2024,
Номер
14(12), С. 4598 - 4621
Опубликована: Янв. 1, 2024
Over
the
past
ten
years,
significant
advancements
have
been
made
in
exploring
plant-derived
exosome-like
nanoparticles
(PELNs)
for
disease
therapeutics
and
drug
delivery.
PELNs,
as
inherent
nanoscale
particles
comprised
of
proteins,
lipids,
nucleic
acids,
secondary
metabolites,
exhibit
capacity
cellular
uptake
by
human
cells.
This
intercellular
interaction
transcends
biological
boundaries,
effectively
influencing
functions
animals.
PELNs
outstanding
biocompatibility,
low
immunogenicity,
enhanced
safety,
environmentally
friendly
sustainability.
article
summarized
preparation
methods
characteristics
PELNs.
It
provided
a
systematic
review
varied
roles
derived
from
fruits,
vegetables,
herbs
The
challenges
their
production
application
were
discussed,
future
prospects
this
rapidly
evolving
field
explored.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(2), С. 1154 - 1154
Опубликована: Янв. 18, 2024
Dramatic
shifts
in
global
climate
have
intensified
abiotic
and
biotic
stress
faced
by
plants.
Plant
microRNAs
(miRNAs)—20–24
nucleotide
non-coding
RNA
molecules—form
a
key
regulatory
system
of
plant
gene
expression;
playing
crucial
roles
growth;
development;
defense
against
stress.
Moreover,
they
participate
cross-kingdom
communication.
This
communication
encompasses
interactions
with
other
plants,
microorganisms,
insect
species,
collectively
exerting
profound
influence
on
the
agronomic
traits
crops.
article
comprehensively
reviews
biosynthesis
miRNAs
explores
their
impact
growth,
development,
resistance
through
endogenous,
non-transboundary
mechanisms.
Furthermore,
this
review
delves
into
effects
pests.
It
proceeds
to
specifically
discuss
design
modification
strategies
for
artificial
(amiRNAs),
as
well
protection
transport
exosome-like
nanovesicles
(ELNVs),
expanding
potential
applications
crop
breeding.
Finally,
current
limitations
associated
harnessing
are
addressed,
utilization
synthetic
biology
is
proposed
facilitate
heterologous
expression
large-scale
production
miRNAs.
novel
approach
suggests
plant-based
solution
address
future
biosafety
concerns
agriculture.
Vaccines,
Год журнала:
2024,
Номер
12(2), С. 200 - 200
Опубликована: Фев. 15, 2024
Nucleic
acid
delivery
through
extracellular
vesicles
(EVs)
is
a
well-preserved
evolutionary
mechanism
in
all
life
kingdoms
including
eukaryotes,
prokaryotes,
and
plants.
EVs
naturally
allow
horizontal
transfer
of
native
as
well
exogenous
functional
mRNAs,
which
once
incorporated
are
protected
from
enzymatic
degradation.
This
observation
has
prompted
researchers
to
investigate
whether
different
sources,
plants,
could
be
used
for
vaccine
delivery.
Several
studies
using
human
or
bacterial
expressing
mRNA
recombinant
SARS-CoV-2
proteins
showed
induction
humoral
cell
mediated
immune
response.
Moreover,
EV-based
vaccines
presenting
the
natural
configuration
viral
antigens
have
demonstrated
advantages
conferring
long-lasting
immunization
lower
toxicity
than
synthetic
nanoparticles.
Edible
plant-derived
were
shown
an
alternative
delivery,
especially
via
oral
administration.
obtained
orange
juice
(oEVs)
loaded
with
mRNAs
their
cargo
degradation,
stable
at
room
temperature
one
year,
able
trigger
response
mice.
Lyophilized
oEVs
containing
S1
administered
rats
gavage
induced
specific
generation
blocking
antibodies,
IgA
Th1
lymphocyte
activation.
In
conclusion,
mRNA-containing
developing
new
due
optimal
mucosal
absorption,
resistance
stress
conditions,
ability
stimulate
cellular
Cells,
Год журнала:
2023,
Номер
12(14), С. 1826 - 1826
Опубликована: Июль 11, 2023
mRNA-based
vaccines
were
effective
in
contrasting
SARS-CoV-2
infection.
However,
they
presented
several
limitations
of
storage
and
supply
chain,
their
parenteral
administration
elicited
a
limited
mucosal
IgA
immune
response.
Extracellular
vesicles
(EVs)
have
been
recognized
as
mechanism
cell-to-cell
communication
well-preserved
all
life
kingdoms,
including
plants.
Their
membrane
confers
protection
from
enzyme
degradation
to
encapsulated
nucleic
acids
favoring
transfer
between
cells.
In
the
present
study,
EVs
derived
juice
an
edible
plant
(Citrus
sinensis)
(oEVs)
investigated
carriers
orally
administered
mRNA
vaccine
coding
for
S1
protein
subunit
with
gastro-resistant
oral
capsule
formulation.
The
loaded
into
oEVs
was
protected
stable
at
room
temperature
one
year
after
lyophilization
encapsulation.
Rats
immunized
via
gavage
developed
humoral
response
production
specific
IgM,
IgG,
IgA,
which
represent
first
barrier
adaptive
vaccination
also
triggered
generation
blocking
antibodies
lymphocyte
activation.
conclusion,
formulation
lyophilized
mRNA-containing
represents
efficient
delivery
strategy
due
stability
temperature,
optimal
absorption,
ability
trigger
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Ноя. 24, 2023
Abstract
Tissue
damage
and
aging
lead
to
dysfunction,
disfigurement,
trauma,
posing
significant
global
challenges.
Creating
a
regenerative
microenvironment
resist
external
stimuli
induce
stem
cell
differentiation
is
essential.
Plant-derived
nanovesicles
(PDNVs)
are
naturally
bioactive
lipid
bilayer
that
contain
proteins,
lipids,
ribonucleic
acid,
metabolites.
They
have
shown
potential
in
promoting
growth,
migration,
into
various
types
of
tissues.
With
immunomodulatory,
microbiota
regulatory,
antioxidant,
anti-aging
bioactivities,
PDNVs
valuable
resisting
facilitating
tissue
repair.
The
unique
structure
provides
an
optimal
platform
for
drug
encapsulation,
surface
modifications
enhance
their
stability
specificity.
Moreover,
by
employing
synergistic
administration
strategies,
can
maximize
therapeutic
potential.
This
review
summarized
the
progress
prospects
as
tools,
provided
insights
selection
repair
activities
based
on
existing
studies,
considered
key
challenge
clinical
application,
anticipated
continued
prominent
role
field
biomedicine.
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