Generating parallel representations of position and identity in the olfactory system

Cell, Год журнала: 2023, Номер 186(12), С. 2556 - 2573.e22

Опубликована: Май 25, 2023

In Drosophila, a dedicated olfactory channel senses male pheromone, cis-vaccenyl acetate (cVA), promoting female courtship while repelling males. Here, we show that separate cVA-processing streams extract qualitative and positional information. cVA sensory neurons respond to concentration differences in 5-mm range around male. Second-order projection encode the angular position of by detecting inter-antennal concentration, which are amplified through contralateral inhibition. At third circuit layer, identify 47 cell types with diverse input-output connectivity. One population responds tonically flies, second is tuned looming, integrates taste coincidentally promote mating. The separation features resembles mammalian what where visual streams; together multisensory integration, this enables behavioral responses appropriate specific ethological contexts.

Язык: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

BMC Bioinformatics, Год журнала: 2024, Номер 25(1)

Опубликована: Март 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Язык: Английский

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Common principles for odour coding across vertebrates and invertebrates

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(7), С. 453 - 472

Опубликована: Май 28, 2024

Язык: Английский

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A split-GAL4 driver line resource for Drosophila neuron types

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

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Neurotransmitter Classification from Electron Microscopy Images at Synaptic Sites in Drosophila Melanogaster

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2020, Номер unknown

Опубликована: Июнь 13, 2020

Abstract High-resolution electron microscopy of nervous systems enables the reconstruction connectomes. A key piece missing information from connectomes is synaptic sign. We show that for D. melanogaster , artificial neural networks can predict transmitter type released at synapses micrographs and thus add putative signs to connections. Our network discriminates between six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) with an average accuracy 87%/94% synapses/entire neurons. developed explainability method reveal which features our using found significant ultrastructural differences classical transmitters. in two characterize morphological connection properties tens thousands neurons classed by predicted expression. find hemilineages largely express only one fastacting among their Furthermore, we different may differ like polarization projection targets.

Язык: Английский

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Connectomics Analysis Reveals First-, Second-, and Third-Order Thermosensory and Hygrosensory Neurons in the Adult Drosophila Brain

Current Biology, Год журнала: 2020, Номер 30(16), С. 3167 - 3182.e4

Опубликована: Июль 2, 2020

Animals exhibit innate and learned preferences for temperature humidity—conditions critical their survival reproduction. Leveraging a whole-brain electron microscopy volume, we studied the adult Drosophila melanogaster circuitry associated with antennal thermo- hygrosensory neurons. We have identified two new target glomeruli in lobe, addition to five known ones, ventroposterior projection neurons (VP PNs) that relay information higher brain centers, including mushroom body lateral horn, seats of behavior. present first connectome neuropil, accessory calyx (lACA), by reconstructing downstream heating- cooling-responsive VP PNs. A few body-intrinsic solely receive thermosensory input from lACA, while most additional olfactory and/or PN inputs. Furthermore, several classes lACA-associated form local network outputs other neuropils, suggesting lACA serves as hub circuitry. For example, DN1a link PNs circadian clock via medulla. Finally, survey strongly connected partners across protocerebrum; these include descending neuron targeted dry-responsive PNs, meaning just synapses might separate inputs motor circuits. These data provide comprehensive first- second-order layer analysis systems an initial third-order could directly modulate

Язык: Английский

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Functional and anatomical specificity in a higher olfactory centre

eLife, Год журнала: 2019, Номер 8

Опубликована: Май 21, 2019

Most sensory systems are organized into parallel neuronal pathways that process distinct aspects of incoming stimuli. In the insect olfactory system, second order projection neurons target both mushroom body, required for learning, and lateral horn (LH), proposed to mediate innate behavior. Mushroom body form a sparse population code, which is not stereotyped across animals. contrast, odor coding in LH remains poorly understood. We combine genetic driver lines, anatomical functional criteria show Drosophila has ~1400 >165 cell types. Genetically labeled LHNs have responses animals on average respond three times more odors than single neurons. better categorizers neurons, likely due pooling related inputs. Our results reveal some principles by higher processing area can extract behavioral significance from

Язык: Английский

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Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain

eLife, Год журнала: 2021, Номер 10

Опубликована: Сен. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Язык: Английский

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Olfactory coding in honeybees

Cell and Tissue Research, Год журнала: 2021, Номер 383(1), С. 35 - 58

Опубликована: Янв. 1, 2021

Abstract With less than a million neurons, the western honeybee Apis mellifera is capable of complex olfactory behaviors and provides an ideal model for investigating neurophysiology circuit basis perception learning. Here, we review most fundamental aspects honeybee’s olfaction: first, discuss which odorants dominate its environment, how bees use them to communicate regulate colony homeostasis; then, describe neuroanatomy circuit; finally, explore cellular molecular mechanisms leading memory formation. The vastity histological, neurophysiological, behavioral data collected during last century, together with new technological advancements, including genetic tools, confirm as attractive research understanding coding

Язык: Английский

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Valence and State-Dependent Population Coding in Dopaminergic Neurons in the Fly Mushroom Body

Current Biology, Год журнала: 2020, Номер 30(11), С. 2104 - 2115.e4

Опубликована: Май 7, 2020

Neuromodulation permits flexibility of synapses, neural circuits, and ultimately behavior. One neuromodulator, dopamine, has been studied extensively in its role as a reward signal during learning memory across animal species. Newer evidence suggests that dopaminergic neurons (DANs) can modulate sensory perception acutely, thereby allowing an to adapt behavior decision making internal behavioral state. In addition, some data indicate DANs are not homogeneous but rather convey different types information heterogeneous population. We have investigated DAN population activity how it could encode relevant about stimuli state by taking advantage the confined anatomy innervating mushroom body (MB) fly Drosophila melanogaster. Using vivo calcium imaging custom 3D image registration method, we found MB encodes innate valence odor or taste well physiological animal. Furthermore, is strongly correlated with movement, consistent dopamine conveying MB. Altogether, our analysis suggest activities valence, MB-compartment-specific manner. propose shapes through combinatorial coding physiological, context.

Язык: Английский

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