Connectome-driven neural inventory of a complete visual system

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 18, 2024

Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial requires a large network of neurons, that in distant flies humans, regions comprise half brain's volume. These brain often reveal remarkable structure-function relationships, neurons organized along maps shapes directly relate to roles processing. To unravel stunning diversity complex system, careful mapping neural architecture matched tools for targeted exploration circuitry is essential. Here, we report new connectome right optic lobe from male Drosophila central nervous system FIB-SEM volume comprehensive inventory fly's neurons. We developed computational framework quantify anatomy establishing basis interpreting how vision. By integrating this analysis connectivity information, neurotransmitter identity, expert curation, classified ~53,000 into 727 types, which are systematically described named first time. Finally, share an extensive collection split-GAL4 lines our neuron type catalog. Together, set data unlock possibilities systematic investigations vision Drosophila, foundation deeper understanding sensory

Язык: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Опубликована: Янв. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

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Connectome-driven neural inventory of a complete visual system

Nature, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies catalogued detail cell types fly systems 1–3 , behavioural physiological experiments examined capabilities flies. To unravel diversity complex system, careful mapping neural architecture matched tools for targeted exploration this circuitry is essential. Here we present connectome right optic lobe male Drosophila melanogaster acquired using focused ion beam milling scanning electron microscopy. We established comprehensive inventory developed computational framework quantify anatomy. Together, data establish basis interpreting how vision. By integrating analysis connectivity information, neurotransmitter identity expert curation, classified approximately 53,000 into 732 types. are systematically described newly named. Finally, share an extensive collection split-GAL4 lines our neuron-type catalogue. Overall, set unlocks new possibilities systematic investigations vision foundation deeper understanding sensory

Язык: Английский

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Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions

Open Biology, Год журнала: 2022, Номер 12(7)

Опубликована: Июль 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Язык: Английский

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Synaptic connectome of the Drosophila circadian clock

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Язык: Английский

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Transcriptomic DN3 clock neuron subtypes regulate Drosophila sleep

Science Advances, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 3, 2025

Circadian neurons within animal brains orchestrate myriad physiological processes and behaviors, but the contribution of these to regulation sleep is not well understood. To address this deficiency, we leveraged single-cell RNA sequencing generate a comprehensive census transcriptomic cell types Drosophila clock neurons. We focused principally on enigmatic DN3s, which constitute most fly brain were previously almost completely uncharacterized. These DN3s are organized into 12 clusters with unusual gene expression features compared more well-studied further show that uncharacterized DN3 subtypes promote through G protein–coupled receptor, TrissinR . Our findings indicate an intricate behavior by highlight their remarkable diversity in functional properties.

Язык: Английский

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Mutual coupling of neurons in the circadian master clock: what we can learn from fruit flies

Neurobiology of Sleep and Circadian Rhythms, Год журнала: 2025, Номер 18, С. 100112 - 100112

Опубликована: Янв. 18, 2025

Circadian master clocks in the brain consist of multiple neurons that are organized into populations with different morphology, physiology, and neuromessenger content presumably functions. In most animals, these distributed bilaterally, located close proximity to visual system, synchronized by eyes light-dark cycles environment. mammals cockroaches, each two consists a core region receives information from shell which output projections originate, whereas flies several other insects, lateral dorsal regions. all cases, morning evening clock seem exist, communication between them neurons, as well connection across hemispheres, is prerequisite for normal rhythmic function. Phenomena such rhythm splitting, internal desynchronization caused "decoupling" hemispheres or decoupling certain within one hemisphere. Since contain relatively few characterized at individual level, fly particularly suited study neurons. Here, we review organization bilateral brain, focus on synaptic paracrine connections comparison insects mammals.

Язык: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Опубликована: Фев. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

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Neural mechanisms of parasite-induced summiting behavior in ‘zombie’ Drosophila

eLife, Год журнала: 2023, Номер 12

Опубликована: Май 15, 2023

For at least two centuries, scientists have been enthralled by the "zombie" behaviors induced mind-controlling parasites. Despite this interest, mechanistic bases of these uncanny processes remained mostly a mystery. Here, we leverage

Язык: Английский

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Synaptic connectome of theDrosophilacircadian clock

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 13, 2023

The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain novel insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated the first comprehensive connectivity map of an animal using Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ∼240 instead 150 neurons. We also revealed extensive contralateral synaptic within discovered indirect light input to Interestingly, observed sparse monosynaptic between neurons down-stream higher-order centers neurosecretory cells known regulate behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Язык: Английский

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