Balancing Bioresponsive Biofilm Eradication and Guided Tissue Repair via Pro-Efferocytosis and Bidirectional Pyroptosis Regulation during Implant Surgery

ACS Nano, Journal Year: 2024, Volume and Issue: 18(20), P. 13196 - 13213

Published: May 8, 2024

There is an increasingly growing demand to balance tissue repair guidance and opportunistic infection (OI) inhibition in clinical implant surgery. Herein, we developed a nanoadjuvant for all-stage biofilm-responsive OI eradication via situ incorporating Cobaltiprotoporphyrin (CoPP) into Prussian blue (PB) prepare PB-CoPP nanozymes (PCZs). Released CoPP possesses pro-efferocytosis effect eliminating apoptotic progressing necrotic cells trauma, thus preventing secondary inflammation. Once OIs occur, PCZs with switchable nanocatalytic capacity can achieve bidirectional pyroptosis regulation. reaching the acidic biofilm microenvironment, possess peroxidase (POD)-like activity that generate reactive oxygen species (ROS) eradicate bacterial biofilms, especially when synergized photothermal effect. Furthermore, generated ROS promote macrophage secrete inflammatory cytokines antimicrobial proteins vivo. After eradicating biofilm, catalase (CAT)-like neutral environment, which scavenge inhibit pyroptosis, thereby improving microenvironment. Briefly, as nanoadjuvants feature capability of be routinely performed all surgeries, providing wide range application prospects commercial translational value.

Language: Английский

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Evaluating the antibacterial and antibiofilm activities of chitosan derivatives containing six-membered heterocyclics against E. coli and S. aureus

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 242, P. 114084 - 114084

Published: July 14, 2024

Language: Английский

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Bacterial Programmed Cell Death Induced by Nanotherapeutic Strategies

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(9), P. 4209 - 4229

Published: Aug. 19, 2024

Programmed cell death (PCD) is crucial for renewal, embryogenesis, the immune response, tissue growth regulation, and other essential biological processes. Recent evidence underscores potential of harnessing PCD to combat bacterial infections, particularly in eradicating antibiotic-resistant superbugs. Extensive efforts have been devoted developing PCD-mediated anti-infective agents by drawing insights from materials science, chemistry, immunology, microbiology. In this review, challenges addressing infections PCD-based approaches revolutionize treatment are first summarized discussed. Then, a comprehensive examination nanoantibacterial therapy, encompassing various pathways, such as apoptosis, ferroptosis, cuproptosis, immunogenic death, NETosis, autophagy, pyroptosis, provided. Finally, barriers prospects PCD-driven antimicrobial strategies explored.

Language: Английский

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Polypyrrole@MnO2 fluorescence nanocomposites as a multifunctional architecture for glutathione sensing and synergistic therapy of cancer

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112852 - 112852

Published: Jan. 1, 2025

Language: Английский

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Bioinspired programmed antibiofilm strategies for accelerated wound healing via spatiotemporally controlled enzyme nanoreactors

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113582 - 113582

Published: March 1, 2025

Language: Английский

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Peroxidase-like nanozyme based microenvironment-responsive injectable hydrogel for bacteria-infected wound healing

Science China Technological Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Language: Английский

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Freeze‐Thaw Microfluidic System Produces “Themis” Nanocomplex for Cleaning Persisters‐Infected Macrophages and Enhancing Uninfected Macrophages

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(18)

Published: Jan. 5, 2024

Abstract Macrophages are the primary effectors against potential pathogen infections. They can be “parasitized” by intracellular bacteria, serving as “accomplices”, protecting bacteria and even switching them to persisters. Here, using a freeze‐thaw strategy‐based microfluidic chip, “Themis” nanocomplex (TNC) is created. The TNC consists of Lactobacillus reuteri ‐derived membrane vesicles, heme, vancomycin, which cleaned infected macrophages enhanced uninfected macrophages. In macrophages, releases heme that led reconstruction respiratory chain complexes persisters, forcing regrow. revived produces virulence factors destroyed host (accomplices), thereby being externalized becoming vulnerable immune responses. upregulates TCA cycle oxidative phosphorylation (OXPHOS), contributing immunoenhancement. combined effect cleaning accomplice (infected macrophages) reinforcing provides promising strategy for bacterial therapy.

Language: Английский

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A novel antibacterial immune activator: Bi-MOF acts as H2S scavenger to suppress HIF-1α S-sulfhydration and alleviate implant-associated infection

Nano Today, Journal Year: 2024, Volume and Issue: 57, P. 102334 - 102334

Published: June 11, 2024

Language: Английский

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Biofilm Microenvironment‐Sensitive Anti‐Virulent and Immunomodulatory Nano‐on‐Nanodroplets to Combat Refractory Biofilm Infection Through Toxin Neutralization and Phagocytosis

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Biofilm‐associated wound infection is principally perceived as the bacterial defense mechanism that hinders antibiotic penetration, causes toxin impairment, and suppresses immunological responses of host immune system. Several antibiofilm agents have been developed, but least these can simultaneously cornerstone on biofilm‐associated immunosuppression toxin‐induced cellular dysfunction. Inspired by fusogenic property nanodroplets immunomodulatory functions metal nanoparticles, biofilm targeted anti‐virulent cationic nanoparticle shelled (C‐AgND) fabricated to completely disintegrate eradicate Staphylococcus aureus (S. aureus) biofilm. The specific binding C‐AgND neutralizes negatively charged EPS layer, causing their destabilization followed penetration nanoformulation into matrix, killing persister cells. Consequently, eliminates virulence S. through α‐hemolysin neutralization. promotes a strong effect polarizing macrophages M1 phenotype induce phagocytosis disintegrated biofilm‐released residual cells, rejuvenating host's innate for complete eradication Moreover, ex vivo skin model illustrates an excellent efficacy in comparison commercial ones, rendering them be promising replacement existing clinical application.

Language: Английский

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Photothermal Nano‐Immunotherapy Against Methicillin‐Resistant Staphylococcus aureus (MRSA) Biofilm Infections

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Methicillin-resistant Staphylococcus aureus (MRSA) biofilm infections are a prevalent type of biofilm-associated infection with poor prognosis and antibiotic resistance. The senescence immune cells in the microenvironment contributes to formation. In this study, Ti₃C₂ MXene-PVA nanosheets loaded metformin (Met@TiC) developed for treatment MRSA infections. Nanosheets utilize near-infrared light induce photothermal effects provide direct bactericidal activity against structures. Met, which is known its anti-inflammatory anti-senescence properties, modulates responses by revitalizing function senescent macrophages within microenvironment, thereby enhancing their phagocytic biofilm-eradicating capabilities. efficacy nanoplatform both vitro an mouse model, demonstrating potential as nanoimmunotherapy combating validated. summary, Met@TiC offers significant alternative clinical solutions

Language: Английский

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