APL Bioengineering, Journal Year: 2021, Volume and Issue: 5(2)
Published: April 5, 2021
One of the key challenges in engineering three-dimensional tissue constructs is development a mature microvascular network capable supplying sufficient oxygen and nutrients to tissue. Recent angiogenic therapeutic strategies have focused on vascularization constructed tissue, its integration
Language: Английский
Advanced Functional Materials, Journal Year: 2020, Volume and Issue: 30(37)
Published: June 11, 2020
From micro-scaled capillaries to millimeter-sized arteries and veins, human vasculature spans multiple scales cell types. The convergence of bioengineering, materials science, stem biology has enabled tissue engineers recreate the structure function different hierarchical levels vascular tree. Engineering large-scale vessels been pursued over past thirty years replace or bypass damaged arteries, arterioles, venules, their routine application in clinic may become a reality near future. Strategies engineer meso- microvasculature have extensively explored generate models study biology, drug transport, disease progression, as well for vascularizing engineered tissues regenerative medicine. However, bioengineering whole organs transplantation, failed result clinical translation due lack proper integrated effective oxygen nutrient delivery. development strategies multi-scale networks direct anastomosis host would greatly benefit this formidable goal. In review, we discuss design considerations technologies engineering millimeter-, meso-, micro-scale vessels. We further provide examples recent state-of-the-art vasculature. Finally, identify key challenges limiting vascularized offer our perspective on future directions exploration.
Language: Английский
Citations
141
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 231, P. 115271 - 115271
Published: March 31, 2023
Current in-vitro 2D cultures and animal models present severe limitations in recapitulating human physiopathology with striking discrepancies estimating drug efficacy side effects when compared to trials. For these reasons, microphysiological systems, organ-on-chip multiorgans microdevices attracted considerable attention as novel tools for high-throughput high-content research achieve an improved understanding of diseases accelerate the development process towards more precise eventually personalized standards. This review takes form a guide on this fast-growing field, providing useful introduction major themes indications further readings. We start analyzing Organs-on-chips (OOC) technologies testing administration routes: (1) oral/rectal route by intestine-on-a-chip, (2) inhalation lung-on-a-chip, (3) transdermal skin-on-a-chip (4) intravenous through vascularization models, considering how drugs penetrate bloodstream are conveyed their targets. Then, we focus OOC (other) specific organs diseases: neurodegenerative brain blood barriers, tumor including vascularization, organoids/spheroids, engineering screening antitumor drugs, liver/kidney chips multiorgan gastrointestinal metabolic assessment biomechanical systems heart, muscles bones structures related diseases. Successively, discuss materials organ chips, microfluidic organs-on-chips, sensor integration real-time monitoring, cell lines chips. (Nano)delivery approaches therapeutics chip also described. Finally, conclude critical discussion current significance/relevance, trends, limitations, challenges future prospects terms revolutionary impact biomedical research, preclinical development.
Language: Английский
Citations
70
Materials & Design, Journal Year: 2024, Volume and Issue: 240, P. 112853 - 112853
Published: March 19, 2024
3D bioprinting techniques have emerged as a flexible tool in tissue engineering and regenerative medicine to fabricate or pattern functional bio-structures with precise geometric designs, bridging the divergence between engineered natural constructs. A significantly increasing development has been achieved understanding relationship 3D-printing process structures, properties, applications of objects created. The ongoing advancement novel biomaterial inks enabled manufacturing models vitro implants capable achieving some level success preclinical trials. Remarkable progress cell biology biology-inspired computational design assisted latest milestone planned tissue- organ-like constructs having specific levels functionality. However, biofabricated still long way go before reaching clinics. This review presents picture context medicine, focus on biomaterials-related design-centred aspects. Biomedical are described detail relation major tissues organs considered human body. Current technical limitations, challenges, future prospects improvements critically outlined discussed.
Language: Английский
Citations
43
Advanced Functional Materials, Journal Year: 2020, Volume and Issue: 31(8)
Published: Nov. 12, 2020
Abstract Nanoparticle transport across tumor blood vessels is a key step in nanoparticle delivery to solid tumors. However, the specific pathways and mechanisms of this process are not fully understood. Here, biological physical characteristics vasculature microenvironment explored how these features affect discussed. The methods deliver nanoparticles into tumors reviewed paracellular transcellular explored. Understanding underlying will inform engineering safer more effective nanomedicines for clinical translation.
Language: Английский
Citations
71
ACS Applied Materials & Interfaces, Journal Year: 2021, Volume and Issue: 13(12), P. 13920 - 13933
Published: March 19, 2021
Tissue barriers play a crucial role in human physiology by establishing tissue compartmentalization and regulating organ homeostasis. At the interface between extracellular matrix (ECM) flowing fluids, epithelial endothelial are responsible for solute gas exchange. In past decade, microfluidic technologies organ-on-chip devices became popular as vitro models able to recapitulate these biological barriers. However, conventional devices, cell primarily grown on hard polymeric membranes within polydimethylsiloxane (PDMS) channels that do not mimic cell–ECM interactions nor allow incorporation of other cellular compartments such stromal or vascular structures. To develop accurately account different acellular barriers, researchers have integrated hydrogels into setups barrier-on-chips, either substrates inside chip, self-contained devices. These biomaterials provide soft mechanical properties embedding cells. Combining with microfluidics technology provides unique opportunities better recreate barrier including components functionality vivo tissues. Such platforms potential greatly improving predictive capacities systems applications drug development, disease modeling. Nevertheless, their development is without challenges microfabrication. this review, we will discuss recent advances driving fabrication hydrogel multiple models.
Language: Английский
Citations
63
Bioactive Materials, Journal Year: 2021, Volume and Issue: 9, P. 316 - 331
Published: Aug. 14, 2021
Three-dimensional (3D) matrix models using hydrogels are powerful tools to understand and predict cell behavior. The interactions between the its matrix, however is highly complex: has a profound effect on basic functions but simultaneously, cells able actively manipulate properties. This (mechano)reciprocity extracellular (ECM) central in regulating tissue it fundamentally important broadly consider biomechanical properties of vivo ECM when designing vitro models. manuscript discusses two commonly used biopolymer networks, i.e. collagen fibrin gels, one synthetic polymer network, polyisocyanide gel (PIC), which all possess characteristic nonlinear mechanics biological stress regime. We start from structure materials, then address uses, advantages, limitations each material, provide guideline for engineers biophysicists utilizing current materials also new 3D culture purposes.
Language: Английский
Citations
57
Advanced Healthcare Materials, Journal Year: 2021, Volume and Issue: 10(21)
Published: July 26, 2021
3D bioprinting is an emerging additive manufacturing technique to fabricate constructs for human disease modeling. However, current cell-laden bioinks lack sufficient biocompatibility, printability, and structural stability needed translate this technology preclinical clinical trials. Here, a new class of nanoengineered hydrogel-based introduced, that can be printed into 3D, anatomically accurate, multicellular blood vessels recapitulate both the physical chemical microenvironments native vasculature. A remarkably unique characteristic bioink regardless cell density, it demonstrates high printability ability protect encapsulated cells against shear forces in process. bioprinted maintain healthy phenotype remain viable nearly one-month post-fabrication. Leveraging these properties, cylindrical vessels, consisting living co-culture endothelial vascular smooth muscle cells, providing opportunity model function pathophysiology. Upon cytokine stimulation perfusion, vessel able thromboinflammatory responses observed only advanced vitro models or vivo. Therefore, provides potential tool understand pathophysiology assess therapeutics, toxins, other chemicals.
Language: Английский
Citations
56
Science Advances, Journal Year: 2021, Volume and Issue: 7(23)
Published: June 2, 2021
Bioinspired nanovesicles enable activated platelet–targeted delivery of tissue plasminogen activator for effective thrombolysis.
Language: Английский
Citations
51
Cells, Journal Year: 2021, Volume and Issue: 10(3), P. 577 - 577
Published: March 5, 2021
Cells exert, sense, and respond to the different physical forces through diverse mechanisms translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as maintain tissue morphogenesis homeostasis activate critical signaling pathways regulating survival, migration, gene expression, differentiation. More importantly, any mutations receptors can lead disorders diseases. Thus, it essential understand regulation cell during development its contribution conditions with help quantitative methods. techniques involved offering functionalities surface imaging detect present at cell-matrix deliver parameters will characterize changes for Here, we have briefly reviewed single-cell mechanical properties mechanotransduction studies using standard recently developed techniques. This used functionalize from measurement cellular deformability quantification interaction generated by a exerted on surroundings attachment detachment events. adhesive force microorganisms single-molecules emphasized well. focused review should be useful laying out experiments which would bring method broader range research future.
Language: Английский
Citations
45
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 13, 2024
Abstract The development of personalized precision medicine has become a pivotal focus in modern healthcare. Organoids‐on‐a‐Chip (OoCs), groundbreaking fusion organoid culture and microfluidic chip technology, emerged as promising approach to advancing patient‐specific treatment strategies. In this review, the diverse applications OoCs are explored, particularly their role medicine, potential cutting‐edge technology is highlighted. By utilizing patient‐derived organoids, offer pathway optimize treatments, create precise disease models, investigate mechanisms, conduct drug screenings, individualize therapeutic emphasis on significance technological revolutionizing healthcare improving patient outcomes. Furthermore, transformative future prospects, ongoing advancements field, with genomic multi‐omics integration, ethical frameworks discussed. convergence these innovations can empower patients, redefine approaches, shape
Language: Английский
Citations
7