Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
12(26)
Published: April 28, 2023
Abstract
Spinal
cord
injury
(SCI)
is
a
devastating
condition
with
no
curative
therapy
currently
available.
Immunomodulation
can
be
applied
as
therapeutic
strategy
to
drive
alternative
immune
cell
activation
and
promote
proregenerative
microenvironment.
Locally
injected
hydrogels
carrying
immunotherapeutic
cargo
directly
injured
tissue
offer
an
encouraging
treatment
approach
from
immunopharmacological
perspective.
Gelatin
methacrylate
(GelMA)
are
promising
in
this
regard,
however,
detailed
analysis
on
the
immunogenicity
of
GelMA
specific
context
SCI
microenvironment
lacking.
Here,
formulated
translationally
relevant
photoinitiator
analyzed
vitro
ex
vivo.
3%
(
w/v
)
GelMA,
synthesized
gelatin
type‐A,
first
identified
optimal
hydrogel
formulation
based
mechanical
properties
cytocompatibility.
Additionally,
GelMA‐A
does
not
alter
expression
profile
key
polarization
markers
BV2
microglia
or
RAW264.7
macrophages
after
48
h.
Finally,
it
shown
for
time
that
support
vivo
culture
primary
murine
organotypic
spinal
slices
14
days
direct
effect
glial
fibrillary
acidic
protein
(GFAP
+
astrocyte
ionized
calcium‐binding
adaptor
molecule
1
(Iba‐1
reactivity.
This
provides
evidence
act
hydrogel‐based
platform
preclinical
SCI.
Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
19(4), P. 042008 - 042008
Published: May 20, 2024
Gelatin
methacryloyl
(GelMA)
hydrogels
have
gained
significant
recognition
as
versatile
biomaterials
in
the
biomedical
domain.
GelMA
emulate
vital
characteristics
of
innate
extracellular
matrix
by
integrating
cell-adhering
and
metalloproteinase-responsive
peptide
motifs.
These
features
enable
cellular
proliferation
spreading
within
GelMA-based
hydrogel
scaffolds.
Moreover,
displays
flexibility
processing,
it
experiences
crosslinking
when
exposed
to
light
irradiation,
supporting
development
with
adjustable
mechanical
characteristics.
The
drug
delivery
landscape
has
been
reshaped
hydrogels,
offering
a
favorable
platform
for
controlled
sustained
release
therapeutic
actives.
tunable
physicochemical
precise
modulation
kinetics
release,
ensuring
optimal
effectiveness.
In
tissue
engineering,
perform
an
essential
role
design
scaffold,
providing
biomimetic
environment
conducive
cell
adhesion,
proliferation,
differentiation.
Incorporating
three-dimensional
printing
further
improves
its
applicability
developing
complicated
constructs
spatial
precision.
Wound
healing
applications
showcase
bioactive
dressings,
fostering
microenvironment
regeneration.
inherent
biocompatibility
provide
efficiency
closure
wounds
repair.
stand
at
forefront
innovation,
addressing
diverse
challenges
delivery,
wound
healing.
This
review
provides
comprehensive
overview,
in-depth
understanding
hydrogel's
potential
impact
on
progressing
sciences.
Composites Part B Engineering,
Journal Year:
2024,
Volume and Issue:
280, P. 111455 - 111455
Published: April 23, 2024
The
reconstruction
of
the
vascular
network
is
crucial
step
in
bone
regeneration.
Therefore,
effectively
modulating
angiogenesis-osteogenesis
coupling
tissue
engineering
scaffolds
currently
an
urgent
need.
In
this
study,
we
employed
silane
agents
containing
double
bonds
to
modify
tetrahedral
silicate,
resulting
preparation
a
photocurable
precursor
45S5
bioactive
glass
(PG).
PG
was
utilized
as
binding
agent
for
tricalcium
phosphate
(TCP)
powder,
and
one-step
photocuring
3D
printing
approach
fabricate
PG/TCP
(PT)
scaffolds.
Furthermore,
endothelial
progenitor
cell-derived
exosomes
(EPC-exos)
encapsulated
by
GelMA
anchored
onto
PT
create
exosome-functionalized
PT/G@Exos
composite
summary,
scaffold
orchestrates
creation
vascularized
regeneration
microenvironment
releasing
EPC-exos,
well
calcium,
silicon
(Si),
phosphorus
(P)
elements.
This
enables
efficient
modulation
accelerates
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Jan. 6, 2025
Recovery
from
spinal
cord
injury
(SCI)
is
often
impeded
by
neuroinflammation,
scar
formation,
and
limited
axonal
regeneration.
To
tackle
these
issues,
we
developed
an
innovative
biomimetic
drug
delivery
system
using
liquid
nitrogen-treated
M2
macrophages
(LNT
M2)
which
internalized
paclitaxel
(PTX)
nanoparticles
beforehand.
These
were
incorporated
into
a
gelatin
methacryloyl
(GelMA)
scaffold,
creating
multifunctional,
injectable
treatment
for
single-dose
administration.
The
LNT
inherited
the
inflammatory
factor/chemokine
receptors
living
thus
possessing
significant
neutralizing
effect.
In
addition,
scaffold
provides
slow,
sustained
release
of
PTX,
promoting
regeneration
suppressing
formation
in
SCI
rats.
M2-based
dual-functional
significantly
enhances
motor
function,
reduces
accelerates
modulating
microenvironment
preventing
glial
fibrotic
scars.
This
approach
combines
regenerative
effects
low-dose
PTX
with
immunoregulatory
properties
M2,
leading
to
remarkable
neurological
recovery
Moreover,
scaffold's
straightforward
preparation,
ease
standardization,
"ready-to-use"
nature
make
it
promising
candidate
acute
intervention
future
clinical
applications.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
33, P. 424 - 443
Published: Nov. 28, 2023
Spinal
cord
injury
(SCI)
causes
severe
axon
damage,
usually
leading
to
permanent
paraparesis,
which
still
lacks
effective
regenerative
therapy.
Recent
studies
have
suggested
that
exosomes
derived
from
neural
stem
cells
(NSCs)
may
hold
promise
as
attractive
candidates
for
SCI
treatment.
Epidermal
Growth
Factor
Receptor
positive
NSC
(EGFR+NSC)
is
a
subpopulation
of
endogenous
NSCs,
showing
strong
capability
in
central
nervous
system
disease.
In
the
current
study,
we
isolated
EGFR+NSCs
(EGFR+NSCs-Exos)
and
discovered
local
delivery
EGFR+NSCs-Exos
can
effectively
promote
neurite
regrowth
site
spinal
cord-injured
mice
improve
their
neurological
function
recovery.
Using
miRNA-seq,
firstly
characterized
microRNAs
(miRNAs)
cargo
identified
miR-34a-5p
was
highly
enriched
exosomes.
We
further
interpreted
exosomal
could
be
transferred
neurons
inhibit
HDAC6
expression
by
directly
binding
its
mRNA,
contributing
microtubule
stabilization
autophagy
induction
aiding
repair.
Overall,
our
research
demonstrated
novel
therapeutic
approach
improving
functional
recovery
using
secreted
NSCs
providing
precise
cell-free
treatment
strategy
Biomacromolecules,
Journal Year:
2023,
Volume and Issue:
24(7), P. 2957 - 2981
Published: June 23, 2023
Over
the
past
two
decades,
hydrogels
have
come
to
forefront
of
tissue
engineering
and
regenerative
medicine
due
their
biocompatibility,
tunable
degradation
low
immunogenicity.
Due
porosity
polymeric
network
built
up,
it
is
possible
incorporate
inside
drugs,
bioactive
molecules,
or
other
biochemically
active
monomers.
Among
biopolymers
used
for
fabrication
functional
hydrogels,
silk
fibroin
(SF)
has
received
considerable
research
attention
owing
its
known
biocompatibility
range
mechanical
properties.
However,
relatively
simple
structure
limits
potential
usability.
One
emerging
strategies
a
chemical
functionalization
SF,
allowing
introduction
methacrylate
groups.
This
allows
versatile
processing
capability,
including
photo-cross-linking,
which
makes
SF
useful
polymer
as
bioink
3D
printing.
The
methacrylation
reaction
been
done
using
numerous
monomers
such
methacrylic
anhydride
(MA),
2-isocyanatoethyl
(IEM),
glycidyl
(GMA).
In
this
Review,
we
summarize
materials
resulting
physicochemical
More
specifically,
brief
explanation
different
methods,
cross-linking
principles,
possibilities,
limitations
compound
are
provided.
addition,
describe
types
link
design
principles
performance
in
applications
broad
fields
biofabrication,
engineering,
medicine.
We
anticipate
that
provided
guidelines
will
contribute
future
development
composites
by
providing
rational
new
mechanisms
linked
successful
realization
targeted
biomedical
application.
Biofabrication,
Journal Year:
2024,
Volume and Issue:
16(3), P. 035015 - 035015
Published: April 2, 2024
Spinal
cord
injury
(SCI)
can
cause
permanent
impairment
to
motor
or
sensory
functions.
Pre-cultured
neural
stem
cell
(NSC)
hydrogel
scaffolds
have
emerged
as
a
promising
approach
treat
SCI
by
promoting
anti-inflammatory
effects,
axon
regrowth,
and
function
restoration.
Here,
in
this
study,
we
performed
coaxial
extrusion
process
fabricate
core-shell
microfiber
with
high
NSC
density
the
core
portion.
Oxidized
hyaluronic
acid,
carboxymethyl
chitosan,
matrigel
blend
were
used
matrix
for
growth
facilitate
fabrication
process.
During
thein
vitrodifferentiation
culture,
it
was
found
that
microfibers
could
differentiate
into
neurons
astrocytes
higher
efficiency
compared
cultured
petri
dishes.
Furthermore,
duringin
vivotransplantation,
coated
polylactic
acid
nanosheets
electrospinning
reinforcement.
The
nanofibers
exhibited
effect
lesion
cavity
filling
rate
control
group.
Meanwhile,
more
neuron-
oligodendrocyte-like
cells
visualized
at
epicenter.
Finally,
regrowth
across
whole
site
observed,
demonstrating
guide
renascent
regrowth.
Experiment
results
indicate
is
bioactive
treatment
complete
superior
outcomes.
Theranostics,
Journal Year:
2023,
Volume and Issue:
14(2), P. 510 - 527
Published: Dec. 5, 2023
Spinal
cord
injury
(SCI)
results
in
neural
tissue
damage.
However,
the
limited
regenerative
capacity
of
adult
mammals'
axons
upon
SCI
leads
to
persistent
neurological
dysfunction.
Thus,
exploring
pathways
that
can
enhance
axon
regeneration
injured
spinal
is
great
significance.
