Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 5, 2024
Abstract
Injectable
porous
microspheres
represent
a
promising
therapeutic
platform
for
cell
delivery,
drug
and
tissue
regeneration.
Yet,
the
engineering
of
silk
fibroin
with
highly
interconnected
structure
remains
an
unsolved
challenge.
In
this
study,
simple
efficient
method
is
developed
that
does
not
require
use
organic
solvents
to
prepare
predictable
structure.
Through
extensive
screening,
addition
glucose
found
direct
formation
from
interior
surface
microspheres.
Compared
(SF
microspheres)
produced
through
combination
electro‐spray,
cryopreservation,
freeze
drying,
fibroin‐glucose
(SF‐Glu
demonstrates
enhanced
capabilities
in
promoting
adhesion
proliferation
vitro.
Both
SF‐Glu
SF
exhibit
capacity
maintain
sustained
release
kinetics
loaded
model
drug.
Furthermore,
facilitate
recruitment
endogenous
cells,
capillary
migration,
macrophage
phenotype
switch
following
subcutaneous
injection
rats.
This
study
opens
new
avenue
construction
microspheres,
which
could
lead
broader
range
applications
regenerative
medicine.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
13(22)
Published: May 21, 2024
The
repair
and
regeneration
of
cartilage
has
always
been
a
hot
topic
in
medical
research.
Cartilage
organoids
(CORGs)
are
special
tissue
created
using
engineering
techniques
outside
the
body.
These
engineered
tissues
provide
models
that
simulate
complex
biological
functions
cartilage,
opening
new
possibilities
for
regenerative
medicine
treatment
strategies.
However,
it
is
crucial
to
establish
suitable
matrix
scaffolds
cultivation
CORGs.
In
recent
years,
utilizing
hydrogel
culture
stem
cells
induce
their
differentiation
into
chondrocytes
emerged
as
promising
method
vitro
construction
this
review,
methods
establishing
CORGs
summarized
an
overview
advantages
limitations
matrigel
such
provided.
Furthermore,
importance
ECM
alternative
substitutes
Matrigel,
alginate,
peptides,
silk
fibroin,
DNA
derivatives
discussed,
pros
cons
these
hydrogels
outlined.
Finally,
challenges
future
directions
research
discussed.
It
hoped
article
provides
valuable
references
design
development
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(28)
Published: May 31, 2024
Abstract
3D
printing
of
articular
cartilage
tissue
faces
challenges
like
replicating
its
complex
structure,
time‐consuming
in
vitro
stem
cell
culture,
and
a
lack
robust
situ
regeneration
methods
for
osteochondral
defects
(OC).
In
response,
an
innovative
approach
utilizing
pre‐designed
bioink
modular
units
one‐step
immediate
implantation
is
proposed,
circumventing
the
need
prior
cultivation.
The
resulting
printed
scaffold
not
only
accurately
reproduces
three‐layer
structure
material
gradient
but
also
attains
impressive
compressive
strength
(6.3
MPa)
through
reinforcement
hydroxyapatite
nanofibers
establishment
chemical
bonds
with
hydrogels.
Moreover,
integrates
capturing
homing
layers
on
bottom
top
via
crosslinking
aptamer
loading
poly
(lactic‐co‐glycolic
acid)
(PLGA)
nanospheres
encapsulated
stromal
cell‐derived
factor‐1α
(SDF‐1α),
respectively.
This
design
enables
specific
capture
bone
marrow
mesenchymal
cells
(BMSCs)
vivo
interaction,
followed
by
their
mobilization
to
home
hyaline
layer
chemotaxis
SDF‐1α
concentration
gradient.
Within
scaffold's
microenvironment,
these
BMSCs
undergo
differentiation
into
distinct
each
layer,
effectively
contributing
repair
OC
rabbits.
Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 16, 2024
Abstract
Stem
cell‐related
therapeutic
technologies
have
garnered
significant
attention
of
the
research
community
for
their
multi‐faceted
applications.
To
promote
effects
stem
cells,
strategies
cell
microencapsulation
in
hydrogel
microparticles
been
widely
explored,
as
potential
to
facilitate
oxygen
diffusion
and
nutrient
transport
alongside
ability
crucial
cell‐cell
cell‐matrix
interactions.
Despite
promise,
there
is
an
acute
shortage
automated,
standardized,
reproducible
platforms
further
research.
Microfluidics
offers
intriguing
platform
produce
cell‐laden
(SCHMs)
owing
its
manipulate
fluids
at
micrometer
scale
well
precisely
control
structure
composition
microparticles.
In
this
review,
typical
biomaterials
crosslinking
methods
microfluidic
encapsulation
cells
progress
droplet‐based
microfluidics
fabrication
SCHMs
are
outlined.
Moreover,
important
biomedical
applications
highlighted,
including
regenerative
medicine,
tissue
engineering,
scale‐up
production
microenvironmental
simulation
fundamental
studies.
Overall,
holds
tremendous
enabling
diverse
worthy
various
Frontiers in Chemistry,
Journal Year:
2024,
Volume and Issue:
12
Published: May 22, 2024
Recent
years
have
witnessed
a
surge
in
the
application
of
microrobots
within
medical
sector,
with
hydrogel
standing
out
due
to
their
distinctive
advantages.
These
microrobots,
characterized
by
exceptional
biocompatibility,
adjustable
physico-mechanical
attributes,
and
acute
sensitivity
biological
environments,
emerged
as
pivotal
tools
advancing
applications
such
targeted
drug
delivery,
wound
healing
enhancement,
bio-imaging,
precise
surgical
interventions.
The
capability
navigate
perform
tasks
complex
systems
significantly
enhances
precision,
efficiency,
safety
therapeutic
procedures.
Firstly,
this
paper
delves
into
material
classification
properties
compares
advantages
different
materials.
Furthermore,
it
offers
comprehensive
review
principal
categories
recent
innovations
synthesis,
actuation
mechanisms,
biomedical
hydrogel-based
microrobots.
Finally,
manuscript
identifies
prevailing
obstacles
future
directions
microrobot
research,
aiming
furnish
insights
that
could
propel
advancements
field.
Journal of Materials Chemistry B,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Vanadium
is
a
bioactive
trace
element
with
variable
valence.
Its
pentavalent
form
has
been
confirmed
to
be
capable
of
predominantly
regulating
the
early
and
mid-stage
osteogenic
differentiation
bone
marrow
mesenchymal
stem
cells
(BMSCs)
without
tumor
inhibition,
while
its
tetravalent
exhibits
inhibition
but
only
primarily
modulates
late
angiogenesis.
In
this
study,
multifunctional
tissue
scaffold
consisting
mixed-valence
vanadium-doped
mesoporous
glass
poly(lactic-co-glycolic
acid)
(V(IV/V)-MBG/PLGA)
was
developed
simultaneously
inhibit
recurrence
osteosarcoma
promote
regeneration
operative
defects.
The
in
vitro
results
showed
that
V(IV)
V(V)
species
could
sustainably
released
from
V(IV/V)-MBG
complementarily
enhance
proliferation,
differentiation,
mineralization
BMSCs
by
activating
multiple
signaling
pathways
throughout
whole
osteogenesis
process.
More
importantly,
co-existence
mixed-valent
vanadium
able
continuously
stimulate
generation
excessive
ROS
depletion
GSH
synergistically
supplying
an
appropriate
ratio
thermodynamically
kinetically
maintain
stable
self-circulation
valence
state
alteration,
thus
inducing
UMR-106
cell
death.
rat
model,
V(IV/V)-MBG/PLGA
scaffolds
effectively
suppressed
invasion
promoted
regeneration.
These
suggest
are
promising
strategy
for
treating
tumor-associated
defects,
offering
dual
