A 3D Bioprinted Human Neurovascular Unit Model of Glioblastoma Tumor Growth

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(15)

Published: Feb. 23, 2024

Abstract A 3D bioprinted neurovascular unit (NVU) model is developed to study glioblastoma (GBM) tumor growth in a brain‐like microenvironment. The NVU includes human primary astrocytes, pericytes and brain microvascular endothelial cells, patient‐derived cells (JHH‐520) are used for this study. Fluorescence reporters with confocal high content imaging quantitate real‐time network formation growth. Extensive validation of the NVU‐GBM immunostaining relevant cellular markers extracellular matrix components; single cell RNA sequencing (scRNAseq) establish physiologically transcriptomics changes; secretion GBM‐relevant cytokines. scRNAseq reveals changes gene expression cytokines associated wound healing/angiogenesis, including appearance an mesenchymal transition population. test 18 chemotherapeutics anti‐cancer drugs assess pharmacological relevance robustness throughput screening.

Language: Английский

---

Gastric Cancer Models Developed via GelMA 3D Bioprinting Accurately Mimic Cancer Hallmarks, Tumor Microenvironment Features, and Drug Responses

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Current in vitro models for gastric cancer research, such as 2D cell cultures and organoid systems, often fail to replicate the complex extracellular matrix (ECM) found vivo. For first time, this study utilizes a gelatin methacryloyl (GelMA) hydrogel, biomimetic ECM-like material, 3D bioprinting construct physiologically relevant model. GelMA's tunable mechanical properties allow precise manipulation of cellular behavior within physiological ranges. Genetic phenotypic analyses indicate that bioprinted GelMA (3Db) model accurately mimics clinical tumor characteristics reproduces key hallmarks, proliferation, invasion, migration, angiogenesis, Warburg effect. Comparisons gene expression drug responses between 3Db patient-derived xenograft models, both constructed from primary cells, validate model's relevance. The ability closely simulate vivo conditions highlights its crucial role identifying treatment targets predicting patient-specific responses, showcasing potential high-throughput screening applications. This is report pivotal GelMA-based advancing research regenerative medicine.

Language: Английский

---

Recent Advances in Hydrogel-Based 3D Disease Modeling and Drug Screening Platforms

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

---

Recent advances in 3D printing applications for CNS tumours

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 7, 2025

Three-dimensional printing (3DP) has emerged as a transformative technology in the field of central nervous system (CNS) tumours, offering innovative advancements various aspects diagnosis, treatment and education. By precisely replicating microenvironment CNS modelling tumour vascularisation, capturing genetic heterogeneity, 3DP enables development targeted therapies personalised strategies. The markedly enhanced preoperative planning intraoperative guidance, providing highly accurate, patient-specific models that improve localisation, facilitate tailored surgical planning, offer superior visualisation complex anatomical structures. Furthermore, revolutionised education training for neurosurgeons, trainees, patients by delivering realistic simulations enhance skills decision-making. Despite its potential, widespread adoption faces challenges, including material biocompatibility issues, high costs, technical limitations. ethical regulatory landscape clinical practice requires further development. This review concludes while offers significant promise advancing treatment, ongoing research is essential to address these challenges optimising impact.

Language: Английский

---

Construction of Multicellular Neural Tissue Using Three-Dimensional Printing Technology: Cell Interaction

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

The study of the human nervous system remains challenging due to its inherent complexity and difficulty in obtaining original samples. Three-dimensional (3D) bioprinting is a rapidly evolving technology field tissue engineering that has made significant contributions several disciplines, including neuroscience. In order more accurately reflect intricate multicellular milieu vivo environment, an increasing number studies have commenced experimentation with coprinting diverse cell types. This article provides overview technical details application 3D multiple types neuroscience, focusing on challenges research conducted based printing. review discusses interactions systems, stem applications, construction brain-like organoids, establishment disease models, potential for integrating other culture techniques.

Language: Английский

---

Precision Spatial Control of Tumor‐Stroma Interactions in Cancer Models via 3D Bioprinting for Advanced Research and Therapy

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract As an emerging technology for modeling cancer tissue in vitro, 3D bioprinting facilitates precise spatial manipulation of cells and biomaterials, allowing the replication complex architectures accurate recapitulation tumor microenvironment (TME) features. This review initially elucidates mechanisms underlying tumorigenesis associated TME complexity, with a particular focus on roles stromal cell populations characteristics extracellular matrix (ECM) progression. Moreover, this work summarizes recent advances bioprinted models, emphasizing their application studying cell‐ECM interactions, stromal‐tumor crosstalk, vasculature formation investigating occurrence metastasis, as well utility high‐throughput drug screening therapeutic development. Finally, discusses advantages challenges creating biomimetic while providing insights into future development trajectories potential translational applications research personalized medicine. By focusing these critical dimensions, aims to highlight transformative role advancing vitro models ultimate goal improving treatment, prevention, patient outcomes.

Language: Английский

---

A comprehensive update on the application of high-throughput fluorescence imaging for novel drug discovery

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

High-throughput fluorescence imaging (HTFI) is revolutionizing drug discovery by enabling rapid and precise detection of biological targets cellular processes. Recent advances in technologies now provide unprecedented sensitivity, resolution, throughput. Integration artificial intelligence (AI) machine learning (ML) into HTFI workflows significantly enhances data processing, aiding hit identification, pattern recognition, mechanistic understanding. This review outlines recent technological developments, integration strategies, emerging applications HTFI. It emphasizes HTFI's role phenotypic screening, especially for complex diseases such as cancer, neurodegenerative disorders, viral infections. Additionally, it highlights 3D culture systems, organoids, organ-on-a-chip technologies, which facilitate physiologically relevant testing, improved predictive accuracy, translational potential, alongside innovative molecular probes biosensors. Despite its advancements, faces ongoing challenges, including standardization, with multi-omics approaches, scalability advanced models. However, progress organoid modeling has enhanced the physiological relevance assays, complemented sophisticated AI ML-driven analysis techniques.

Language: Английский

---

Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11000 - 11000

Published: Oct. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Language: Английский

---

Advancements in Cancer Research: 3D Models, Single‐Cell, and Live‐Cell Techniques for Better Insights

Advanced Therapeutics, Journal Year: 2024, Volume and Issue: 7(12)

Published: Nov. 9, 2024

Abstract The present review provides a comprehensive overview of the current state in vitro cancer studies, focusing on recent advancements and ongoing cell culture models analyses techniques. Cancer cells grow complex dynamic environment, interacting with various cellular components, such as stromal cells, cancer‐associated fibroblasts, immune extracellular matrix (ECM). ECM structural support unique characteristics essential for tumorigenesis. Accurately modeling this intricate tumor microenvironment precisely analyzing cell–cell cell–ECM interactions are crucial understanding progression therapeutic responses. Consequently, oncology research is advancing toward a) three‐dimensional models, b) single‐cell level analyses, c) live‐cell analyses. This aims to elucidate knowledge field, emphasizing benefits these innovative approaches offer over traditional two‐dimensional bulk endpoint measurements.

Language: Английский

---

Innovative drug delivery strategies for targeting glioblastoma: overcoming the challenges of the tumor microenvironment

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Introduction - Glioblastoma multiforme(GBM) presents a challenging endeavor in therapeutic management because of its highly aggressive tumor microenvironment(TME). This complex TME, characterized by hypoxia, nutrient deprivation, immunosuppression, stromal barriers, increased interstitial fluid pressure and the presence blood-brain barrier(BBB), frequently compromises efficacy promising strategies. Consequently, deeper understanding TME development innovative methods to overcome associated challenges are essential for improving treatment outcomes GBM.

Language: Английский

---