Molecular Probes for Autofluorescence-Free Optical Imaging

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(21), P. 13086 - 13131

Published: Sept. 24, 2021

Optical imaging is an indispensable tool in clinical diagnostics and fundamental biomedical research. Autofluorescence-free optical imaging, which eliminates real-time excitation to minimize background noise, enables clear visualization of biological architecture physiopathological events deep within living subjects. Molecular probes especially developed for autofluorescence-free have been proven remarkably improve the sensitivity, penetration depth, target specificity, multiplexing capability. In this Review, we focus on advancements molecular through lens particular or photophysical mechanisms that produce long-lasting luminescence after cessation light excitation. The versatile design strategies these are discussed along with a broad range applications. Finally, challenges perspectives further advance next-generation vivo vitro biosensors.

Language: Английский

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A generic approach towards afterglow luminescent nanoparticles for ultrasensitive in vivo imaging

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: May 2, 2019

Abstract Afterglow imaging with long-lasting luminescence after cessation of light excitation provides opportunities for ultrasensitive molecular imaging; however, the lack biologically compatible afterglow agents has impeded exploitation in clinical settings. This study presents a generic approach to transforming ordinary optical (including fluorescent polymers, dyes, and inorganic semiconductors) into luminescent nanoparticles (ALNPs). integrates cascade photoreaction single-particle entity, enabling ALNPs chemically store photoenergy spontaneously decay it an energy-relay process. Not only can profiles be finetuned afford emission from visible near-infrared (NIR) region, but also their intensities predicted by mathematical model. The representative NIR permit rapid detection tumors living mice signal-to-background ratio that is more than three orders magnitude higher fluorescence. biodegradability further heightens potential vivo imaging.

Language: Английский

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Conjugated Polymer Nanoparticles for Imaging, Cell Activity Regulation, and Therapy

Advanced Functional Materials, Journal Year: 2018, Volume and Issue: 29(5)

Published: Nov. 29, 2018

Abstract Conjugated polymer nanoparticles, considered as a class of promising nanomaterials for biomedical application, are extensively employed in bioimaging, anti‐microorganism and antitumor, gene drug delivery/release the past decade. By virtue unique photoelectric properties, such strong light absorbing quality, high brightness, good photostability, tunable spectra property, favorable compatibility, conjugated nanoparticles attract increasing attention used more emerging aspects biological fields. This review summarizes recent (2014–2018) development including design, synthesis, applications. Especially, their abilities cell activity regulation, antitumor therapy discussed detail. Finally, challenges outlooks field highlighted.

Language: Английский

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Photoactivatable Organic Semiconducting Pro-nanoenzymes

Journal of the American Chemical Society, Journal Year: 2019, Volume and Issue: 141(9), P. 4073 - 4079

Published: Feb. 11, 2019

Therapeutic enzymes hold great promise for cancer therapy; however, in vivo remote control of enzymatic activity to improve their therapeutic specificity remains challenging. This study reports the development an organic semiconducting pro-nanoenzyme (OSPE) with a photoactivatable feature metastasis-inhibited therapy. Upon near-infrared (NIR) light irradiation, this not only generates cytotoxic singlet oxygen (1O2) photodynamic therapy (PDT), but also triggers spontaneous cascade reaction induce degradation ribonucleic acid (RNA) specifically tumor microenvironment. More importantly, OSPE-mediated RNA is found downregulate expression metastasis-related proteins, contributing inhibition metastasis after treatment. Such photoactivated and cancer-specific synergistic action OSPE enables complete growth lung mouse xenograft model, which possible counterpart PDT nanoagent. Thus, our proposes phototherapeutic-proenzyme approach toward complete-remission

Language: Английский

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Gathering brings strength: How organic aggregates boost disease phototheranostics

Aggregate, Journal Year: 2021, Volume and Issue: 2(1), P. 95 - 113

Published: Jan. 22, 2021

Abstract Phototheranostics that concurrently and complementarily integrate real‐time diagnosis in situ therapeutic capabilities one platform has become the advancing edge of precision medicine. Organic agents possess merits facile preparation, high purity, tunable photophysical property, good biocompatibility, potential biodegradability, which have shown great promise for disease theranostics. This review summarizes recent achievements organic phototheranostic applications, especially rationally utilize energy dissipation pathways Jablonski diagram to modulate fluorescence emission, photoacoustic/photothermal production, photodynamic processes. Of particular interest are systems exhibiting huge differences aggregate state as compared with solution or single molecule form, during intramolecular motions play an important role regulating properties. The advances from such aspect biomedical applications including high‐resolution imaging, activatable imaging therapy, adaptive theranostics, image‐guided surgery, immunotherapy, afterglow discussed. A brief summary perspective this field also presented. We hope will be helpful researchers interested bioprobe design theranostic inspire new insights into linkage between science field.

Language: Английский

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