Peptide-based coacervates as biomimetic protocells

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(6), P. 3690 - 3705

Published: Jan. 1, 2021

This tutorial review describes molecular design principles for peptides and peptide derivatives undergoing phase separation highlights the potential of resulting coacervate protocells.

Language: Английский

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Engineering protein-based therapeutics through structural and chemical design

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: April 27, 2023

Protein-based therapeutics have led to new paradigms in disease treatment. Projected be half of the top ten selling drugs 2023, proteins emerged as rivaling and, some cases, superior alternatives historically used small molecule-based medicines. This review chronicles both well-established and emerging design strategies that enabled this paradigm shift by transforming protein-based structures are often prone denaturation, degradation, aggregation vitro vivo into highly effective therapeutics. In particular, we discuss for creating with increased affinity targetability, enhanced stability pharmacokinetics, improved cell permeability, reduced amounts undesired immunogenicity.

Language: Английский

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Ultra-strong bio-glue from genetically engineered polypeptides

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 14, 2021

Abstract The development of biomedical glues is an important, yet challenging task as seemingly mutually exclusive properties need to be combined in one material, i.e. strong adhesion and adaption remodeling processes healing tissue. Here, we report a biocompatible biodegradable protein-based adhesive with high strengths. maximum strength reaches 16.5 ± 2.2 MPa on hard substrates, which comparable that commercial cyanoacrylate superglue higher than other adhesives by at least order magnitude. Moreover, the soft tissues qualifies glue outperforming some products. Robust mechanical are realized without covalent bond formation during process. A complex consisting cationic supercharged polypeptides anionic aromatic surfactants lysine surfactant molar ratio 1:0.9 driven multiple supramolecular interactions enabling such adhesion. We demonstrate glue’s robust performance vitro vivo for cosmetic hemostasis applications accelerated wound comparison surgical closures.

Language: Английский

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Sustainable Design of Structural and Functional Polymers for a Circular Economy

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 62(12)

Published: Oct. 5, 2022

To achieve a sustainable circular economy, polymer production must start transitioning to recycled and biobased feedstock accomplish CO2 emission neutrality. This is not only true for structural polymers, such as in packaging or engineering applications, but also functional polymers liquid formulations, adhesives, lubricants, thickeners dispersants. At their end of life, be either collected via technical pathway, biodegradable if they are collectable. Advances chemistry aided by computational material science, open the way addressing these issues comprehensively designing recyclability biodegradability. Review explores how scientific progress, together with emerging regulatory frameworks, societal expectations economic boundary conditions, paint pathways transformation towards economy polymers.

Language: Английский

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An Artificial Phase‐Transitional Underwater Bioglue with Robust and Switchable Adhesion Performance

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(21), P. 12082 - 12089

Published: March 1, 2021

Complex coacervation enables important wet adhesion processes in natural and artificial systems. However, existed synthetic coacervate adhesives show limited properties, non-thermoresponsiveness, inferior biodegradability, greatly hampering their translations. Herein, by harnessing supramolecular assembly rational protein design, we present a temperature-sensitive bioadhesive fabricated through recombinant surfactant. Mechanical performance of the bioglue system is actively tunable with thermal triggers. In cold condition, strength was only about 50 kPa. By increasing temperature, presented up to 600 kPa, which remarkably stronger than other biological counterparts. This probably due thermally triggered phase transition engineered formation coacervate, thus leading enhanced bonding.

Language: Английский

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Significantly Improving the Bioefficacy for Rheumatoid Arthritis with Supramolecular Nanoformulations

Advanced Materials, Journal Year: 2021, Volume and Issue: 33(16)

Published: March 17, 2021

Abstract As a typical inflammatory disease with chronic pain syndromes, rheumatoid arthritis (RA) generally requires long‐term treatment frequent injection administration at 1–2 times per day, because common medications such as interleukin1 receptor antagonist (IL1ra) have poor bioavailability and very limited half‐life residence. Here novel strategy to fabricate nanotherapeutic formulations employing genetically engineered IL1ra protein complexes, yielding ultralong‐lasting bioefficacy is developed rationally. Using rat models, it shown that these nanotherapeutics significantly improved drug regimen single subcutaneous in 14‐day therapy, suggesting their extraordinary ultralong‐acting pharmacokinetics. Specifically, the of nanoformulations are boosted level 30 h by 7 times, respectively, greater than other systems. This new thus holds great promise potently improve patient compliance RA can be adapted for therapies suffer similar drawbacks.

Language: Английский

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Engineering High Strength and Super‐Toughness of Unfolded Structural Proteins and their Extraordinary Anti‐Adhesion Performance for Abdominal Hernia Repair

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(19)

Published: March 9, 2022

The utility of unfolded structural proteins with diverse sequences offers multiple potentials to create functional biomaterials. However, it is challenging overcome their defects for the development biological fibers a combination high strength and toughness. Herein, robust from recombinant protein consisting resilin supercharged polypeptide are fabricated via wet-spinning approaches. Particularly, highly ordered structures induced by supramolecular complexation significantly improve fiber's mechanical performance. In contrast chemical low toughness (or vice versa), present demonstrate exceptional super-toughness, showing breaking ≈550 MPa ≈250 MJ m-3 , respectively, surpassing many polymers artificial fibers. Remarkably, outstanding biocompatibility superior properties allow application constructed fiber patches efficient abdominal hernia repair in rat models. stark clinical patches, there no observed tissue adhesion this treatment. Therefore, work provides new type engineered material surgical applications.

Language: Английский

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Nanopore Detection Using Supercharged Polypeptide Molecular Carriers

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(11), P. 6371 - 6382

Published: March 10, 2023

The analysis at the single-molecule level of proteins and their interactions can provide critical information for understanding biological processes diseases, particularly present in samples with low copy numbers. Nanopore sensing is an analytical technique that allows label-free detection single solution ideally suited to applications, such as studying protein–protein interactions, biomarker screening, drug discovery, even protein sequencing. However, given current spatiotemporal limitations nanopore sensing, challenges remain controlling translocation through a relating structures functions readouts. Here, we demonstrate supercharged unstructured polypeptides (SUPs) be genetically fused interest used molecular carriers facilitate proteins. We show cationic SUPs substantially slow down target due electrostatic surface. This approach enables differentiation individual different sizes shapes via characteristic subpeaks current, thus facilitating viable route use polypeptide control transport potential system study level.

Language: Английский

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Biomimetic Mineralization: From Microscopic to Macroscopic Materials and Their Biomedical Applications

ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(9), P. 3516 - 3531

Published: March 21, 2023

Biomineralization is an attractive pathway to produce mineral-based biomaterials with high performance and hierarchical structures. To date, the biomineralization process mechanism have been extensively studied, especially for formation of bone, teeth, nacre. Inspired by those, abundant biomimetic mineralized materials fabricated biomedical applications. Those bioinspired generally exhibit great mechanical properties biological functions. Nevertheless, substantial gaps remain between natural materials, particularly respect mutiscale This Review summarizes recent progress micro- macroscopic mineralization from perspective synthesis begin with, we discuss at microscopic level. The strength, stability, functionality nano- micromaterials are significantly improved introducing biominerals, such as DNA nanostructures, nanovaccines, living cells. Next, numerous strategies based on scale highlighted, including in situ bottom-up assembly building blocks. Finally, challenges future perspectives regarding development also presented aim offering insights rational design fabrication next-generation materials.

Language: Английский

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Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(3), P. 1850 - 1860

Published: Feb. 28, 2024

Developing double-network (DN) hydrogels with high mechanical properties and antibacterial efficacy to combat multidrug-resistant bacterial infections serve as scaffolds for cell culture still remains an ongoing challenge. In this study, ion-responsive peptide (AMP) (C16-WIIIKKK, termed IK7) was synergistically combined a photoresponsive gelatin methacryloyl (GelMA) polymer fabricate biocompatible DN hydrogel. The GelMA-IK7 hydrogel showed enhanced in contrast the individual IK7 GelMA demonstrated substantial efficacy. Further investigations revealed that effectively inhibited growth by controlled sustained release of peptide. addition, formation also found protect AMP from rapid degradation proteinase K. Our findings suggested developed holds great potential next-generation three-dimensional tissue regeneration.

Language: Английский

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