Suspended Tissue Open Microfluidic Patterning (STOMP)

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Free‐standing tissue structures tethered between pillars are powerful mechanobiology tools for studying cell contraction. To model interfaces ubiquitous in natural tissues and upgrade existing single‐region suspended constructs, we developed Suspended Tissue Open Microfluidic Patterning (STOMP), a method to create multi‐regional tissues. STOMP uses open microfluidics capillary pinning pattern subregions within free‐standing tissues, facilitating the study of complex interfaces, such as diseased‐healthy boundaries (e.g., fibrotic‐healthy) tissue‐type bone‐ligament). We observed altered contractile dynamics fibrotic‐healthy engineered heart compared differing contractility bone‐ligament enthesis constructs single‐tissue periodontal ligament models. is versatile platform – surface tension‐driven patterning removes material requirements common with other methods shear‐thinning, photopolymerizable) allowing generation multiple geometries native extracellular matrices advanced four‐dimensional (4D) materials. combines functionality precise patterning, enabling dynamic spatially controlled studies.

Language: Английский

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Will microfluidics enable functionally integrated biohybrid robots?

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(35)

Published: Aug. 24, 2022

The next robotics frontier will be led by biohybrids. Capable biohybrid robots require microfluidics to sustain, improve, and scale the architectural complexity of their core ingredient: biological tissues. Advances in have already revolutionized disease modeling drug development, are positioned impact regenerative medicine but yet apply Fusing with living materials improve tissue perfusion maturation, enable precise patterning sensing, processing, control elements. This perspective suggests future developments advanced

Language: Английский

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Responsive hydrogel microfibers for biomedical engineering

Smart Medicine, Journal Year: 2022, Volume and Issue: 1(1)

Published: Dec. 1, 2022

Responsive hydrogel microfibers can realize multiple controllable changes in shapes or properties under the stimulation of surrounding environment, and are called as intelligent biomaterials. Recently, these responsive have been proved to possess significant biomedical values, remarkable progress has achieved engineering applications, including drug delivery, biosensors clinical therapy, etc. In this review, latest research application prospects summarized. We first introduce common preparation strategies microfibers. Subsequently, response characteristics applications materials discussed. Finally, present opportunities challenges well for future development critically analyzed.

Language: Английский

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Soft Fiber/Textile Actuators: From Design Strategies to Diverse Applications

ACS Nano, Journal Year: 2023, Volume and Issue: 18(1), P. 89 - 118

Published: Dec. 26, 2023

Fiber/textile-based actuators have garnered considerable attention due to their distinctive attributes, encompassing higher degrees of freedom, intriguing deformations, and enhanced adaptability complex structures. Recent studies highlight the development advanced fibers textiles, expanding application scope fiber/textile-based across diverse emerging fields. Unlike sheet-like soft actuators, fibers/textiles with intricate structures exhibit versatile movements, such as contraction, coiling, bending, folding, achieved through adjustable strain stroke. In this review article, we provide a timely comprehensive overview fiber/textile including structures, fabrication methods, actuation principles, applications. After discussing hierarchical structure deformation actuator, discuss various spinning strategies, detailing merits drawbacks each. Next, present principles fiber/fabric along common external stimuli. addition, summary applications actuators. Concluding an assessment existing challenges future opportunities, aims valuable perspective on enticing realm

Language: Английский

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Living cell‐laden hydrogels: Unleashing the future of responsive biohybrid systems

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(1)

Published: Aug. 1, 2023

Abstract Responsive biohybrid systems have the potential to overcome limitations of both natural and artificial machines in terms efficiency, accuracy, functionality. As functional units, living cells act as bricks for building machines, where extracellular matrix mimics hydrogels ideal biological concrete. Combining with offers unique advantages simulating human tissues or organs, which unleashes future systems, thus has attracted extensive attention. Herein, recent progress cell‐laden hydrogel‐based responsive is summarized provide a basic understanding how these are built from bottom up achieve complex functions. This review focuses on advanced manufacturing technologies including laden hydrogel matrices, three‐dimensional bioprinting, microfluidic manufacturing. Subsequently, innovative applications works, actuators, sensors, engineered materials, presented, along different triggering mechanisms that highlighted. Finally, current challenges opportunities field addressed. provides perspective hoped inspire fields such technologies, soft robots, tissue engineering.

Language: Английский

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Cyclical endometrial repair and regeneration: Molecular mechanisms, diseases, and therapeutic interventions

MedComm, Journal Year: 2023, Volume and Issue: 4(6)

Published: Dec. 1, 2023

Abstract The endometrium is a unique human tissue with an extraordinary ability to undergo hormone‐regulated cycle encompassing shedding, bleeding, scarless repair, and regeneration throughout the female reproductive cycle. cyclical repair of manifest as changes in endometrial epithelialization, glandular regeneration, vascularization. mechanisms encompass inflammation, coagulation, fibrinolytic system balance. However, specific conditions such endometriosis or TCRA treatment can disrupt process regeneration. There uncertainty about traditional clinical treatments' efficacy side effects, finding new therapeutic interventions essential. Researchers have made substantial progress perspective regenerative medicine toward maintaining recent years. Such encompasses integration biomaterials, tissue‐engineered scaffolds, stem cell therapies, 3D printing. This review analyzes mechanisms, diseases, associated discusses advantages disadvantages currently employed practice. Additionally, it highlights significant this domain. Finally, we cells biologics among available medicine, providing insights into future strategies.

Language: Английский

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Advances of 3D Cell Co-Culture Technology Based on Microfluidic Chips

Biosensors, Journal Year: 2024, Volume and Issue: 14(7), P. 336 - 336

Published: July 10, 2024

Cell co-culture technology aims to study the communication mechanism between cells and better reveal interactions regulatory mechanisms involved in processes such as cell growth, differentiation, apoptosis, other cellular activities. This is achieved by simulating complex organismic environment. Such studies are of great significance for understanding physiological pathological multicellular organisms. As an emerging cultivation technology, 3D based on microfluidic chips, can efficiently, rapidly, accurately achieve co-culture. accomplished leveraging unique microchannel structures flow characteristics chips. The simulate native microenvironment providing a new technical platform studying intercellular communication. It has been widely used research oncology, immunology, neuroscience, fields. In this review, we summarize provide insights into design systems detection methods employed systems, applications these models.

Language: Английский

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Mechanical strategies to promote vascularization for tissue engineering and regenerative medicine

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract Vascularization is a major challenge in the field of tissue engineering and regenerative medicine. Mechanical factors have been demonstrated to play fundamental role vasculogenesis angiogenesis can affect architecture generated vascular network. Through regulation mechanical engineered tissues, various strategies be used optimize preformed network promote its rapid integration with host vessels. Optimization properties scaffolds, including controlling scaffold stiffness, increasing surface roughness anisotropic structure, designing interconnected, hierarchical pore structures, beneficial for vitro formation networks ingrowth blood The incorporation hollow channels into scaffolds promotes patterned networks. Dynamic stretching perfusion facilitate maturation vitro. Several indirect provide sustained stimulation tissues vivo, which further vascularization implants within body. Additionally, stiffness gradients, substrates as well external cyclic stretch, boundary constraints dynamic flow culture, effectively regulate alignment networks, thereby promoting better prevascularized This review summarizes influence contribution both scaffold-based stimulus-based elucidates underlying mechanisms involved.

Language: Английский

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Periodic Flows in Microfluidics

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Microfluidics, the science and technology of manipulating fluids in microscale channels, offers numerous advantages, such as low energy consumption, compact device size, precise control, fast reaction, enhanced portability. These benefits have led to applications biomedical assays, disease diagnostics, drug discovery, neuroscience, so on. Fluid flow within microfluidic channels is typically laminar region, which characterized by Reynolds numbers but brings challenge efficient mixing fluids. Periodic flows are time‐dependent fluid flows, featuring repetitive patterns that can significantly improve extend effective length microchannels for submicron nanoparticle manipulation. Besides, periodic crucial organ‐on‐a‐chip (OoC) accurately modeling physiological processes, advancing understanding, development, personalized medicine. Various techniques generating been reported, including syringe pumps, peristalsis, actuation based on electric, magnetic, acoustic, mechanical, pneumatic, fluidic forces, yet comprehensive reviews this topic remain limited. This paper aims provide a review microfluidics, from fundamental mechanisms generation applications. The challenges future perspectives also discussed exploit potential microfluidics.

Language: Английский

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Microfluidic Platforms for Real‐Time In Situ Monitoring of Biomarkers for Cellular Processes

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(6)

Published: Oct. 16, 2023

Abstract Cellular processes are mechanisms carried out at the cellular level that aimed guaranteeing stability of organism they comprise. The investigation is key to understanding cell fate, pathogenic mechanisms, and developing new therapeutic technologies. Microfluidic platforms thought be most powerful tools among all methodologies for investigating because can integrate almost types existing intracellular extracellular biomarker‐sensing methods observation approaches behavior, combined with precisely controlled culture, manipulation, stimulation, analysis. Most importantly, microfluidic realize real‐time in situ detection secreted proteins, exosomes, other biomarkers produced during physiological processes, thereby providing possibility draw whole picture a process. Owing their advantages high throughput, low sample consumption, precise control, monitoring characteristics widely being used analysis, disease diagnosis, pharmaceutical research, biological production. This review focuses on basic concepts, recent progress, application prospects processes.

Language: Английский

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