Integrated Multi-Omics Analysis to Investigate the Molecular Mechanisms Underlying the Response of Auricularia heimuer to High-Temperature Stress

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 167 - 167

Published: Feb. 20, 2025

High-temperature stress is a key factor that reduces the yields of edible fungi. Auricularia heimuer (A. heimuer) nutrient-rich fungus widely cultivated in China. In this study, we analyzed physiological, transcriptomic, and metabolomic results A. (variety “Hei29”) under high-temperature stress. Our findings revealed high temperatures (30 °C 35 °C) significantly reduced hyphal growth, increased malondialdehyde content antioxidant enzyme activity, enhanced accumulation secondary metabolites, such as phenolic compounds flavonoids. A total 15 candidate genes potentially responsive to were identified through transcriptomic analysis, including those involved regulating defense, heat shock response, sugar metabolism, amino acid accumulating metabolites. Metabolomic analysis three metabolites stress, kinetin, flavonoids, caffeic acid, well several metabolic pathways, nucleotide ABC transporters, cofactor biosynthesis. These mechanisms help mitigate oxidative damage cellular structures energy deficits caused by elevated temperatures, enabling maintain stability, function, growth This study first explore molecular mechanism response The provide valuable insights into tolerance heimuer, highlighting potential targets for developing heat-tolerant strains industrial application.

Language: Английский

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Orally Administered Functional Polyphenol‐Nanozyme‐Armored Probiotics for Enhanced Amelioration of Intestinal Inflammation and Microbiota Dysbiosis

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Abstract Maintaining microbiota balance and enhancing the antioxidant performance of nanozyme‐based probiotic systems are crucial for effective inflammatory bowel disease (IBD) therapy. Despite significant advancements, developing a green safe coating technology that functionalizes probiotics with nanozymes while preserving activity both components remains challenge. To address this, chitosan‐modified epigallocatechin gallate (EGCG‐CS, EC)is synthesized, leveraging intrinsic adhesive coordination properties polyphenols to capture gold (AuNPs), forming ECA complexes enhance nanozyme activity. When coated onto Escherichia coli Nissle 1917 (EcN), resulting ECA@EcN system effectively scavenged reactive oxygen species (ROS), improving viability promoting colon accumulation. Mechanistically, protected EcN by suppressing activation Flagellar Assembly Branched‐Chain Amino Acid Synthesis pathways, ultimately alleviating inflammation modulating intestinal microbial communities relieve IBD symptoms. Given biocompatibility its environmentally friendly assembly approach, this polyphenol‐nanozyme‐armored represents promising platform treatment.

Language: Английский

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Recent advances on emerging nanomaterials for diagnosis and treatment of inflammatory bowel disease

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 363, P. 149 - 179

Published: Sept. 27, 2023

Language: Английский

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Orally Administered Silicon Hydrogen Nanomaterials as Target Therapy to Treat Intestinal Diseases

ACS Nano, Journal Year: 2023, Volume and Issue: 17(21), P. 21539 - 21552

Published: Oct. 16, 2023

The occurrence and development of inflammatory bowel diseases (IBDs) are inextricably linked to the excessive production reactive oxygen species (ROS). Thus, there is an urgent need develop innovative tactics combat IBDs scavenge excess ROS from affected areas. Herein, silicon hydrogen nanoparticles (SiH NPs) with ROS-scavenging ability were prepared by etching Si nanowires (NWs) fluoride (HF) alleviate symptoms associated IBD orally targeting inflamed colonic sites. strong reductive Si–H bonds showed excellent stability in gastric intestinal fluids, which exhibited efficient effects protect cells high oxidative stress-induced death. After oral delivery, negatively charged SiH NPs specifically adsorbed positively epithelial tissues colon for extended period via electrostatic interactions prolong residence time. significant preventive therapeutic dextran sodium sulfate-induced prophylactic mouse models inhibiting shortening, reducing secretion pro-inflammatory cytokines, regulating macrophage polarization, protecting barrier. As determined using 16S rDNA high-throughput sequencing, administration treatment led changes abundance microbiome, improved bacterial diversity restored relative beneficial bacteria after colon. Overall, our findings highlight broad application SiH-based anti-inflammatory drugs other diseases.

Language: Английский

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Designing a microbial fermentation-functionalized alginate microsphere for targeted release of 5-ASA using nano dietary fiber carrier for inflammatory bowel disease treatment

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Sept. 23, 2023

Abstract Patients with inflammatory bowel disease (IBD) always suffer from severe abdominal pain and appear to be at high risk for colorectal cancer. Recently, the co-delivery of targeted drugs gut microbiota has developed into an attractive strategy. A new strategy using fermentation overcome interspace diffuse resistance mucus layer control drug release in sites (IBS sites) not yet been available. Here, we designed alginate hydrogel microsphere encapsulating bifidobacterium (Bac) drug-modified nanoscale dietary fibers (NDFs). The is responsible protecting acidic multi-enzymatic environments delivering colorectum. Subsequently, Bac by digesting NDFs proteins as carbon nitrogen sources can promote play a probiotic role microbiota. In vitro evidence indicated that small-sized NDF (NDF-1) could significantly short-chain fatty acid (SCFA) expression. Notably, NDF-1 microspheres showed boost 5-ASA IBS sites, resulting amelioration inflammation remodeling chronic colitis mice. This study controlled system based on microbial treatment IBD.

Language: Английский

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