Development of Granzyme B-targeted Smart Positron Emission Tomography Probes for Monitoring Tumor Early Response to Immunotherapy DOI
Jiayu Fu,

Hongjie Xi,

Shuyue Cai

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(29), P. 18910 - 18921

Published: July 13, 2024

Granzyme B is an immune-related biomarker that closely correlates with cytotoxic T lymphocytes (CTLs), and hence detecting the expression level of granzyme can provide a dependable scheme for clinical immune response assessment. In this study, two positron emission tomography (PET) probes [18F]SF-M-14 [18F]SF-H-14 targeting are designed based on intramolecular cyclization scaffold SF. respond to glutathione (GSH) conduct self-assemble into nanoaggregates enhance retention probe at target site. Both prepared high radiochemical purity (>98%) stability in PBS mouse serum. 4T1 cells cocultured lymphocytes, reach maximum uptake 6.71 ± 0.29 3.47 0.09% ID/mg 0.5 h, respectively, but they remain below 1.95 0.22 1.47 0.21% without coculture lymphocytes. vivo PET imaging shows tumor 4T1-tumor-bearing mice after immunotherapy significantly higher (3.5 times) than untreated group. The treated BEC was 4.08 0.16 3.43 0.12% ID/g, while 1.04 0.79 1.41 0.11% respectively. These results indicate both have great potential early evaluation efficacy.

Language: Английский

Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process DOI

Weijing Huang,

Wenhui Zeng, Zheng Huang

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(26)

Published: April 19, 2024

Ratiometric afterglow luminescent (AGL) probes are attractive for in vivo imaging due to their high sensitivity and signal self-calibration function. However, there currently few ratiometric AGL available enzymatic activity living organisms. Here, we present an energy diversion (ED) strategy that enables the design of enzyme-activated probe (RAG-RGD) imaging. The ED process provides RAG-RGD with a radiative transition 'always on' 520-nm (AGL

Language: Английский

Citations

6
Ultrasound-Induced Cascade Amplification in a Mechanoluminescent Nanotransducer for Enhanced Sono-Optogenetic Deep Brain Stimulation DOI
Wenliang Wang, Kai Wing Kevin Tang, Ilya Pyatnitskiy

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(24), P. 24936 - 24946

Published: Dec. 14, 2023

Remote and genetically targeted neuromodulation in the deep brain is important for understanding treatment of neurological diseases. Ultrasound-triggered mechanoluminescent technology offers a promising approach achieving remote modulation. However, its application has thus far been limited to shallow depths due challenges related low sonochemical reaction efficiency restricted photon yields. Here we report cascaded nanotransducer achieve efficient light emission upon ultrasound stimulation. As result, blue was generated under stimulation with subsecond response latency. Leveraging high energy transfer focused tissue sensitivity these nanotransducers, are able show delivery activation ChR2-expressing neurons both superficial motor cortex ventral tegmental area after intracranial injection. Our liposome nanotransducers enable minimally invasive behavioral control animals via flexible, sono-optogenetic system.

Language: Английский

Citations

15
Hierarchical Self‐Assembly Molecular Building Blocks as Intelligent Nanoplatforms for Ovarian Cancer Theranostics DOI Creative Commons
Shuo Li, Qingrong Chen, Qi Xu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(17)

Published: Feb. 26, 2024

Hierarchical self-assembly from simple building blocks to complex polymers is a feasible approach constructing multi-functional smart materials. However, the polymerization process of often involves challenges such as design and drive external energy. Here, hierarchical with self-driven energy conversion capabilities based on p-aminophenol diethylenetriamine reported. Through β-galactosidase (β-Gal) specific activation self-assembly, intelligent assemblies (oligomer superpolymer) excellent photothermal fluorescent properties are dynamically formed in situ, thus sensitive multi-mode detection β-Gal activity realized. Based overexpression ovarian cancer cells, superpolymer specifically generated SKOV-3 cells achieve fluorescence imaging. The therapeutic ability oligomer (synthesized vitro) evaluated by subcutaneous model, showing satisfactory anti-tumor effects. This work expands construction through cascade assembly small molecules provides new methods for diagnosis treatment cancer.

Language: Английский

Citations

5
Reactive oxygen species-mediated organic long-persistent luminophores light up biomedicine: from two-component separated nano-systems to integrated uni-luminophores DOI
Zhe Li, Hong‐Wen Liu, Xiaobing Zhang

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

An overview of the recent advances in reactive oxygen species-mediated organic long-persistent luminophores, including their history, working mechanisms, design strategies, and biomedical applications.

Language: Английский

Citations

5
Ultrasound activated probe for disease imaging and therapy In-Vivo DOI
Xing Liu,

Jimei Wang,

Ying Wu

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 205, P. 115158 - 115158

Published: Dec. 15, 2023

Language: Английский

Citations

12
Light/X-ray/ultrasound activated delayed photon emission of organic molecular probes for optical imaging: mechanisms, design strategies, and biomedical applications DOI
Rui Qu, Xiqun Jiang, Zhen Xu

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(22), P. 10970 - 11003

Published: Jan. 1, 2024

Versatile energy inputs, including light, X-ray and ultrasound, activate organic molecular probes to undergo different delay mechanisms, charge separation, triplet exciton stabilization chemical trap, for delayed photon emission.

Language: Английский

Citations

4
Highly Photoreactive Semiconducting Polymers with Cascade Intramolecular Singlet Oxygen and Energy Transfer for Cancer-Specific Afterglow Theranostics DOI
Yiguang Lin, Jingsheng Huang, Jing Liu

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Afterglow luminescence provides ultrasensitive optical detection by minimizing tissue autofluorescence and increasing the signal-to-noise ratio. However, due to lack of suitable unimolecular afterglow scaffolds, current agents are nanocomposites containing multiple components with limited performance have rarely been applied for cancer theranostics. Herein, we report synthesis a series oxathiine-containing donor-acceptor block semiconducting polymers (PDCDs) observation their high photoreactivity strong near-infrared (NIR) luminescence. We reveal that PDCDs absorb NIR light undergo photodynamic process generate singlet oxygen (1O2), which intramolecularly transfers efficiently reacts oxathiine form intermediates low Gibbs free energy changes required this photoreaction. Following intramolecular transfer from donor acceptor block, emission is produced PDCDs. Owing efficient cascade photochemical process, PDCD-based nanoparticles achieve higher brightness longer compared most reported agents, even after ultrashort photoirradiation only 3 s. Furthermore, within PDCD can be inhibited bioconjugation quencher-linked peptide. This allows construction cancer-activatable theranostic probe (CATP) switches on signal function in presence cancer-overexpressed enzyme. Thereby, CATP represents first phototheranostic permits cancer-specific therapy under preclinical settings. In summary, study molecular guideline develop probes photoreactive polymers.

Language: Английский

Citations

0
Molecular probes for in vivo optical imaging of immune cells DOI
Jing Liu, Penghui Cheng, Cheng Xu

et al.

Nature Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

0
Non‐Invasive Diagnosis of Early Colorectal Cancerization via Amplified Sensing of MicroRNA‐21 in NIR‐II Window DOI Open Access

Fushan Zhai,

Baofeng Yun,

Ming Jiang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Accurate, sensitive, and in situ visualization of aberrant expression level low‐abundant biomolecules is crucial for early colorectal cancer (CRC) detection ahead tumor morphology change. However, the clinical used colonoscopy biopsy methods are invasive lack sensitivity at early‐stage cancerization. Here, an amplified sensing strategy developed second near‐infrared long‐wavelength subregion (NIR‐II‐L, 1500–1900 nm) by integrating DNAzyme‐triggered signal amplification technology lanthanide‐dye hybrid system. In CRC, overexpressed biomarker microRNA‐21 initiates NIR‐II‐L luminescence ratiometric CRCsensor. The high with a limit (LOD) 1.26 p m allows non‐invasive orthotopic cancerization via rectal administration, which achieves accurate diagnosis 2 weeks vitro histological results. This innovative approach offers promising tool long‐term monitoring carcinogenesis progression, potential applications other cancer‐related biomarkers.

Language: Английский

Citations

0
Noninvasively Real‐Time Monitoring In‐Vivo Immune Cell and Tumor Cell Interaction by NIR‐II Nanosensor DOI Open Access
Liwen Huang, Ming Jiang,

Zhihua Wang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract Immunocytotherapy holds significant promise as a novel cancer treatment, but its effectiveness is often hindered by delayed responses, requiring evaluations every 2–3 weeks based on current diagnostic methods. Early assessment of immune cell‐tumor cell interactions could provide more timely insights into therapeutic efficacy, enabling adjustments to treatment plans. In this study, noninvasive nanosensor (C8R‐DSNP) for real‐time monitoring in vivo activities the second near‐infrared long‐wavelength (NIR‐II‐L) window (1500–1900 nm), which offers deep tissue transparency, reported. The C8R‐DSNP responds rapidly caspase‐8, key apoptotic signaling molecule generated during between natural killer (NK‐92) cells and tumor cells. Using ratiometric NIR‐II‐L fluorescence imaging, dynamic observations NK‐92 cells' engagement with mouse model are captured. These results demonstrate apoptosis that happens early 4.5 h after infusion. Additionally, vitro urine imaging confirmed initiation via cleaved fluorescent small molecules, while single‐cell tracking within blood vessels tumors further elucidated dynamics. This approach valuable optimizing immunocytotherapy strategies.

Language: Английский

Citations

0