Elucidating the catalytic mechanism of Prussian blue nanozymes with self-increasing catalytic activity

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 13, 2024

Abstract Although Prussian blue nanozymes (PBNZ) are widely applied in various fields, their catalytic mechanisms remain elusive. Here, we investigate the long-term performance of PBNZ as peroxidase (POD) and catalase (CAT) mimetics to elucidate lifespan underlying mechanisms. Unlike our previously reported Fe 3 O 4 nanozymes, which exhibit depletable POD-like activity, POD CAT-like activities not only persist but slightly enhance over prolonged catalysis. We demonstrate that irreversible oxidation significantly promotes catalysis, leading self-increasing activities. The process pre-oxidized can be initiated through either conduction band pathway or valence pathway. In summary, reveal follows a dual-path electron transfer mechanism during offering advantage long service life.

Language: Английский

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Multi‐Enzyme Mimetic MoCu Dual‐Atom Nanozyme Triggering Oxidative Stress Cascade Amplification for High‐Efficiency Synergistic Cancer Therapy

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 64(1)

Published: Aug. 22, 2024

Single-atom nanozymes (SAzymes) with ultrahigh atom utilization efficiency have been extensively applied in reactive oxygen species (ROS)-mediated cancer therapy. However, the high energy barriers of reaction intermediates on single-atom sites and overexpressed antioxidants tumor microenvironment restrict amplification oxidative stress, resulting unsatisfactory therapeutic efficacy. Herein, we report a multi-enzyme mimetic MoCu dual-atom nanozyme (MoCu DAzyme) various catalytic active sites, which exhibits peroxidase, oxidase, glutathione (GSH) nicotinamide adenine dinucleotide phosphate (NADPH) oxidase mimicking activities. Compared Mo SAzyme, introduction Cu atoms, formation synergetic effects among enhance substrate adsorption reduce barrier, thereby endowing DAzyme stronger Benefiting from above enzyme-like activities, can not only generate multiple ROS, but also deplete GSH block its regeneration to trigger cascade stress. Additionally, strong optical absorption near-infrared II bio-window endows remarkable photothermal conversion performance. Consequently, achieves high-efficiency synergistic treatment incorporating collaborative therapy This work will advance applications DAzymes provide valuable insights for nanocatalytic

Language: Английский

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Rational Design of Nanozymes for Engineered Cascade Catalytic Cancer Therapy

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Nanozymes have shown significant potential in cancer catalytic therapy by strategically catalyzing tumor-associated substances and metabolites into toxic reactive oxygen species (ROS) situ, thereby inducing oxidative stress promoting cell death. However, within the complex tumor microenvironment (TME), rational design of nanozymes factors like activity, reaction substrates, TME itself significantly influence efficiency ROS generation. To address these limitations, recent research has focused on exploring that affect activity developing nanozyme-based cascade systems, which can trigger two or more processes tumors, producing therapeutic achieving efficient stable with minimal side effects. This area remarkable progress. Perspective provides a comprehensive overview nanozymes, covering their classification fundamentals. The regulation nanozyme strategies are discussed detail. Furthermore, representative paradigms for successful construction systems treatment summarized focus revealing underlying mechanisms. Finally, we current challenges future prospects development biomedical applications.

Language: Английский

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Amino acid-based, sustainable organic nanozyme and integrated sensing platform for histamine detection

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 142751 - 142751

Published: Jan. 1, 2025

Inorganic nanozymes hold promise for biomolecule sensing but face challenges like complex fabrication, toxicity, and low sustainability, limiting their use. To overcome these, a sustainable organic nanozyme (OA nanozyme) was created using amino acids biocompatible polymer effective histamine detection. The OA exhibits peroxidase-like activity fabricated through single chelation/polymer entanglement method, enabling rapid production (within 3 h) with uniform morphology (≤100 nm diameter) negative surface charge at neutral pH. It shows decent kinetic performance (Km = 0.009 mM H

Language: Английский

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Single‐Atom Iridium‐doped Carbon Dots Nanozyme with High Peroxidase‐Like Activity as Colorimetric Sensors for Multimodal Detection of Mercury Ions

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Nanozyme-based colorimetric sensors are promising approaches for environmental monitoring, food safety, and medical diagnostics. However, developing novel nanozymes that exhibit high catalytic activity, good dispersion in aqueous solution, sensitivity, selectivity, stability is challenging. In this study, the first time, single-atom iridium-doped carbon dot (SA Ir-CDs) synthesized via a simple situ pyrolysis process. Doping dots with iridium form of single atoms to achieve maximum atomic utilization not only enhances peroxidase (POD)-like activity 178.81 U mg-1 but also improves dispersibility solutions over 30 days. Hence, SA Ir-CD platform developed mercury ions (Hg2+) detection exhibited linear relationship from 0.01 10 µm limit 4.4 nm. Notably, changes color can be observed through naked eye smartphone, enabling convenient field onsite monitoring without need sophisticated analytical equipment. an approach fabricating metal-based POD-like developed, new effective easy-to-use sensor Hg2+ constructed.

Language: Английский

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