Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(38), P. 14494 - 14501

Published: Sept. 14, 2023

Noble-metal nanozymes have demonstrated great potential in various fields. However, aggregation of single-particle nanoparticles severely affects their exposed catalytically active sites to the extent exhibiting weak enzyme-like activity. Here, we present an organic block surfactant (polyvinylpyrrolidone, PVP) construct monodisperse water-stable Pt nanoclusters (Pt NCs) for enhanced immunoassay cardiac troponin I (cTnI). The PVP-modified NC nanozyme exhibited up 16.3 U mg-1 peroxidase-mimicking activity, which was mainly attributed ligand modification on surface and electron-absorbing effect NCs. NCs a lower OH-transition potential, as determined by density functional theory. Under optimized experimental conditions, strategy ultrawide dynamic response range 0.005-50 ng mL-1 cTnI targets with detection limit 1.3 pg mL-1, far superior some reported test protocols. This work provides designable pathway design artificial enzymes high activity further expand practical enzyme alternatives.

Language: Английский

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Bioinspired porous three-coordinated single-atom Fe nanozyme with oxidase-like activity for tumor visual identification via glutathione

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 6, 2023

Inspired by structures of natural metalloenzymes, a biomimetic synthetic strategy is developed for scalable synthesis porous Fe-N3 single atom nanozymes (pFeSAN) using hemoglobin as Fe-source and template. pFeSAN delivers 3.3- 8791-fold higher oxidase-like activity than Fe-N4 Fe3O4 nanozymes. The high catalytic performance attributed to (1) the suppressed aggregation atomically dispersed Fe; (2) facilitated mass transfer maximized exposure active sites created mesopores thermal removal (2 ~ 3 nm); (3) unique electronic configuration oxygen-to-water oxidation pathway (analogy with cytochrome c oxidase). successfully demonstrated rapid colorimetric detection glutathione low limit (2.4 nM) wide range (50 nM-1 mM), further real-time, facile, (~6 min) precise visualization analysis methodology tumors via level, showing its potentials diagnostic clinic applications.

Language: Английский

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Supramolecular Photothermal Cascade Nano‐Reactor Enables Photothermal Effect, Cascade Reaction, and In Situ Hydrogelation for Biofilm‐Associated Tooth‐Extraction Wound Healing

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(31)

Published: June 7, 2023

Abstract Due to the emergence of drug resistance in bacteria and biofilm protection, achieving a satisfactory therapeutic effect for bacteria‐infected open wounds with conventional measures is problematic. Here, photothermal cascade nano‐reactor (CPNC@GOx‐Fe 2+ ) constructed through supramolecular strategy hydrogen bonding coordination interactions between chitosan‐modified palladium nano‐cube (CPNC), glucose oxidase (GOx), ferrous iron (Fe ). CPNC@GOx‐Fe exhibits excellent effects powers GOx‐assisted reaction generate hydroxyl radicals, enabling chemodynamic combination therapy against biofilms. Further proteomics, metabolomics, all‐atom simulation results indicate that damage radical function structure cell membrane thermal enhance fluidity inhomogeneity bacterial membrane, resulting synergistic antibacterial effect. In biofilm‐associated tooth extraction wound model, generated from process can initiate polymerization form hydrogel situ protection. vivo experiments confirm protection accelerate healing infected tooth‐extraction without affecting oral commensal microbiota. This study provides way propose multifunctional system treatment infection.

Language: Английский

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Nanozymes for nanohealthcare

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: May 30, 2024

Language: Английский

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Deep Insight of Design, Mechanism, and Cancer Theranostic Strategy of Nanozymes

Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 16(1)

Published: Nov. 21, 2023

Since the discovery of enzyme-like activity Fe

Language: Английский

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Nanozymes: Applications in clinical biomarker detection

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(4)

Published: Sept. 19, 2023

Abstract Enzymes are highly efficient catalysts employed in the biomedical field. However, their use is limited by poor stability and high cost. To address this, there an imperative need for research on nanozymes. In last decade, introduction of nanozymes has led to exponential growth nanozyme‐based biosensors. The rapid precise detection clinical biomarkers crucial various disease diagnostics. recent years, researchers have developed different catalytic active nanomaterials combined them with colorimetric, fluorescent, electrochemical methods detect biomarkers. This review critically overviews progress, future prospects, challenges biomarker assays past few years.

Language: Английский

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Atomic-level design of metalloenzyme-like active pockets in metal–organic frameworks for bioinspired catalysis

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 53(1), P. 137 - 162

Published: Nov. 29, 2023

Natural metalloenzymes with astonishing reaction activity and specificity underpin essential life transformations. Nevertheless, enzymes only operate under mild conditions to keep sophisticated structures active, limiting their potential applications. Artificial that recapitulate the catalytic of can not circumvent enzymatic fragility but also bring versatile functions into practice. Among them, metal-organic frameworks (MOFs) featuring diverse site-isolated metal sites supramolecular have emerged as promising candidates for move toward unparalleled properties behaviour enzymes. In this review, we systematically summarize significant advances in MOF-based metalloenzyme mimics a special emphasis on active pocket engineering at atomic level, including primary secondary coordination spheres. Then, deep understanding mechanisms advanced applications are discussed. Finally, perspective emerging frontier research is provided advance bioinspired catalysis.

Language: Английский

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Using Cu‐Based Metal–Organic Framework as a Comprehensive and Powerful Antioxidant Nanozyme for Efficient Osteoarthritis Treatment

Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)

Published: Jan. 26, 2024

Abstract Developing nanozymes with effective reactive oxygen species (ROS) scavenging ability is a promising approach for osteoarthritis (OA) treatment. Nonetheless, numerous lie in their relatively low antioxidant activity. In certain circumstances, some of these may even instigate ROS production to cause side effects. To address challenges, copper‐based metal–organic framework (Cu MOF) nanozyme designed and applied OA Cu MOF exhibits comprehensive powerful activities (i.e., SOD‐like, CAT‐like, •OH activities) while negligible pro‐oxidant (POD‐ OXD‐like activities). Collectively, more at various types than other Cu‐based antioxidants, such as commercial CuO single‐atom nanozyme. Density functional theory calculations also confirm the origin its outstanding enzyme‐like activities. vitro vivo results demonstrate that an excellent decrease intracellular levels relieve hypoxic microenvironment synovial macrophages. As result, can modulate polarization macrophages from pro‐inflammatory M1 anti‐inflammatory M2 subtype, inhibit degradation cartilage matrix efficient The biocompatibility protective properties make it valuable asset treating ROS‐related ailments beyond OA.

Language: Английский

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Bimetallic Nanozyme: A Credible Tag for In Situ-Catalyzed Reporter Deposition in the Lateral Flow Immunoassay for Ultrasensitive Cancer Diagnosis

Nano Letters, Journal Year: 2023, Volume and Issue: 24(1), P. 51 - 60

Published: Oct. 12, 2023

The lateral flow immunoassay (LFIA) is a sought-after point-of-care testing platform, yet the insufficient sensitivity of LFIA limits its application in detection tumor biomarkers. Here, colorimetric signal amplification method, bimetallic nanozyme-mediated situ-catalyzed reporter deposition (BN-ISCRD), was designed for ultrasensitive cancer diagnosis. nanozyme used, palladium@iridium core–shell nanoparticles (Pd@Ir NPs), had ultrahigh enzyme-like activity, which further explained by electron transfer Pd@Ir NPs and change Gibbs free energy during catalysis through density functional theory calculations. With gastric biomarkers pepsinogen I II as model targets, this assay could achieve cutoff value 10 pg/mL, 200-fold lower than that without enhancement. applied to correctly identify 8 positive 28 negative clinical samples. Overall, BN-ISCRD-based showed great merits potential disease

Language: Английский

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Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(12), P. 3470 - 3488

Published: Aug. 15, 2024

Language: Английский

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