Beige adipose tissue-derived extracellular vesicles: a potent metabolic regulator and a novel remedy for nonalcoholic fatty liver disease DOI Open Access
Kai Zhang, Sha Zhang, Bing‐Dong Sui

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 2, 2024

Abstract Adipose tissue (AT) is an essential metabolic and endocrine organ, which are categorized into the white adipose (WAT) brown (BAT), as well beige (BeAT) that derives from WAT browning. Notably, extracellular vesicles (EVs), especially tissue-produced EVs, have been recognized to be critical players in multiple physiological pathophysiological settings provide efficient strategy for disease treatment. However, little known about BeAT-derived EVs (BeEVs). In this study, we induced BeEV formation by cold stimulation extracted three kinds of AT via enzyme digestion combined with gradient centrifugation. The isolated BeEVs possess typical morphological, structural marker characteristics EVs. Proteomics analysis further demonstrated hold a distinct protein profile while enriched regulatory proteins. Importantly, systemic injection mice improved dysfunction nonalcoholic fatty liver (NAFLD) model high-fat diet (HFD). Moreover, alleviated steatosis decreasing lipogenesis. These findings promote understanding AT-EVs shed light on isolation, identification applications potent regulator novel remedy diseases.

Language: Английский

The application of extracellular vesicles in orthopedic diseases DOI Creative Commons
Yuexin Li, Haiyan Zhang, Yu Jiang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(3)

Published: March 13, 2024

Abstract Orthopedic diseases, such as osteoarthritis and fractures, place a significant burden on individuals healthcare systems worldwide. Extracellular vesicles (EVs), which are membrane‐derived particles, have emerged novel tool in the field of orthopedics. EVs play crucial role diagnosing, regenerating, treating orthopedic diseases. In terms diagnosis, serve potential biomarkers, carrying unique donor cell information circulating effectively bodily fluids. Specific biomolecules within EVs, including proteins, nucleic acids, microRNAs, hold promise biological markers for early detection monitoring shown promoting bone cartilage regeneration. They can enhance tissue regeneration by stimulating various stem cells to proliferate, migrate, differentiate into mature chondrocytes osteocytes. also target specific tissues, making them attractive candidates drug delivery efficiently deliver therapeutic cargo, anti‐inflammatory agents growth factors, affected sites, enhancing treatment efficacy while minimizing toxicity adverse effects. conclusion,

Language: Английский

Citations

16
Nutrient availability regulates the secretion and function of immune cell–derived extracellular vesicles through metabolic rewiring DOI Creative Commons
Yizhuo Wang,

Peng Lou,

Yijing Xie

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(7)

Published: Feb. 14, 2024

Extracellular vesicle (EV)-based immunotherapeutics have emerged as promising strategy for treating diseases, and thus, a better understanding of the factors that regulate EV secretion function can provide insights into developing advanced therapies. Here, we report nutrient availability, even changes in individual components, may affect biogenesis composition immune cells [e.g., macrophages (Mφs)]. As proof concept, EVs from M1-Mφ under glutamine-depleted conditions (EV

Language: Английский

Citations

11
High-throughput capture and in situ protein analysis of extracellular vesicles by chemical probe-based array DOI
Xin Feng, Ao Shen, Wei Zhang

et al.

Nature Protocols, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Language: Английский

Citations

9
Emerging Frontiers in acute kidney injury: The role of extracellular vesicles DOI
Sirui Li, Lan Zhou, Yu Huang

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 149 - 170

Published: Feb. 18, 2025

Language: Английский

Citations

1
Prospects and challenges of tissue-derived extracellular vesicles DOI Creative Commons
C. Justin Lee, Roslyn M. Ray, Tristan Scott

et al.

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(9), P. 2950 - 2978

Published: June 22, 2024

Extracellular vesicles (EVs) are considered a vital component of cell-to-cell communication and represent new frontier in diagnostics means to identify pathways for therapeutic intervention. Recently, studies have revealed the importance tissue-derived EVs (Ti-EVs), which present interstitial spaces between cells, as they better underlying physiology complex, multicellular tissue microenvironments biology disease. native, lipid bilayer membraned nano-sized particles produced by all cells that packaged with varied functional biomolecules including proteins, lipids, nucleic acids. They implicated short- long-range cellular may elicit responses recipient cells. To date, often utilized cultured or biological fluids source do not capture local molecular signatures microenvironment. Recent work utilizing Ti-EVs has elucidated novel biomarkers disease provided insights into mechanisms lead development agents. Still, there considerable challenges facing current studies. This review explores vast potential unique Ti-EV research provides considerations future seek advance this exciting field.

Language: Английский

Citations

8
Hepatocyte-derived tissue extracellular vesicles safeguard liver regeneration and support regenerative therapy DOI Creative Commons
Si-Qi Ying, Yuan Cao,

Ze-Kai Zhou

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 30, 2024

Tissue-derived extracellular vesicles (EVs) are emerging as pivotal players to maintain organ homeostasis, which show promise a next-generation candidate for medical use with extensive source. However, the detailed function and therapeutic potential of tissue EVs remain insufficiently studied. Here, through bulk single-cell RNA sequencing analyses combined ultrastructural examinations, we first reveal that in situ liver (LT-EVs) contribute intricate regenerative process after partial hepatectomy (PHx), hepatocytes primary source regenerating liver. Nanoscale proteomic profiling further identify hepatocyte-specific (Hep-EVs) strengthened release carrying proliferative messages PHx. Moreover, targeted inhibition Hep-EV via AAV-shRab27a vivo confirms Hep-EVs required orchestrate regeneration. Mechanistically, from reciprocally stimulate hepatocyte proliferation by promoting cell cycle progression Cyclin-dependent kinase 1 (Cdk1) activity. Notably, supplementing demonstrates translational ameliorates insufficient This study provides functional mechanistic framework showing governs rapid regeneration, thereby enriching our understanding physiological endogenous regeneration therapy.

Language: Английский

Citations

7
Emerging role of extracellular vesicles in veterinary practice: novel opportunities and potential challenges DOI Creative Commons
Yindi Xiong,

Peng Lou,

Chuang Xu

et al.

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 25, 2024

Extracellular vesicles are nanoscale that transport signals between cells, mediating both physiological and pathological processes. EVs facilitate conserved intercellular communication. By transferring bioactive molecules coordinate systemic responses, regulating homeostasis, immunity, disease progression. Given their biological importance involvement in pathogenesis, show promise as biomarkers for veterinary diagnosis, candidates vaccine production, treatment agents. Additionally, different or engineering methods could be used to boost the capability of extracellular vesicles. Despite emerging interest, EV research has been predominantly human-based. Critical knowledge gaps remain regarding isolation protocols, cargo loading mechanisms, vivo biodistribution, species-specific functions. Standardized characterization validation lacking. Regulatory uncertainties impede clinical translation. Advances fundamental biology technology needed propel field forward. This review introduces from a perspective by introducing latest studies, highlighting potential while analyzing challenges motivate expanded investigation

Language: Английский

Citations

6
Exosomal Proteomics: Unveiling Novel Insights into Lung Cancer DOI Creative Commons

Guanhua Yi,

Haixin Luo,

Yalin Zheng

et al.

Aging and Disease, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Although significant progress has been made in early lung cancer screening over the past decade, it remains one of most prevalent and deadliest forms worldwide. Exosomal proteomics emerged as a transformative field research, with potential to redefine diagnostics, prognostic assessments, therapeutic strategies through lens precision medicine. This review discusses recent advances exosome-related proteomic glycoproteomic technologies, highlighting their revolutionise treatment by addressing issues heterogeneity, integrating multiomics data, utilising advanced analytical methods. While these technologies show promise, there are obstacles overcome before they can be widely implemented, such need for standardization, gaps clinical application, importance dynamic monitoring. Future directions should aim challenges fully utilize exosomal cancer. promises new era personalized medicine that leverages molecular complexity exosomes groundbreaking advancements detection, prognosis, treatment.

Language: Английский

Citations

6
A Review of the Use of Extracellular Vesicles in the Treatment of Neonatal Diseases: Current State and Problems with Translation to the Clinic DOI Open Access
Кirill Goryunov, Mikhail Ivanov, А. В. Куликов

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2879 - 2879

Published: March 1, 2024

Neonatal disorders, particularly those resulting from prematurity, pose a major challenge in health care and have significant impact on infant mortality long-term child health. The limitations of current therapeutic strategies emphasize the need for innovative treatments. New cell-free technologies utilizing extracellular vesicles (EVs) offer compelling opportunity neonatal therapy by harnessing inherent regenerative capabilities EVs. These nanoscale particles, secreted variety organisms including animals, bacteria, fungi plants, contain repertoire bioactive molecules with potential. This review aims to provide comprehensive assessment effects EVs mechanistic insights into stem cells, biological fluids non-animal sources, focus common conditions such as hypoxic–ischemic encephalopathy, respiratory distress syndrome, bronchopulmonary dysplasia necrotizing enterocolitis. summarizes evidence potential EVs, analyzes their mechanisms action discusses challenges associated implementation EV-based therapies clinical practice.

Language: Английский

Citations

4
Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and Melanocytes DOI Creative Commons

Sebastian Kistenmacher,

Melanie Schwämmle, Gottfried Martin

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(7), P. 623 - 623

Published: April 4, 2024

Limbal epithelial progenitor cells (LEPC) rely on their niche environment for proper functionality and self-renewal. While extracellular vesicles (EV), specifically small EVs (sEV), have been proposed to support LEPC homeostasis, data sEV derived from limbal like mesenchymal stromal (LMSC) remain limited, there are no studies sEVs melanocytes (LM). In this study, we isolated conditioned media of LMSC LM using a combination tangential flow filtration size exclusion chromatography characterized them by nanoparticle tracking analysis, transmission electron microscopy, Western blot, multiplex bead arrays, quantitative mass spectrometry. The internalization was studied cytometry confocal microscopy. exhibited typical EV characteristics, including cell-specific markers such as CD90 LMSC-sEV Melan-A LM-sEV. Bioinformatics analysis the proteomic suggested significant role in matrix deposition, with LMSC-derived containing proteins involved collagen remodeling cell adhesion, whereas LM-sEV were implicated other cellular bioprocesses pigmentation development. Moreover, fluorescently labeled taken up localized perinuclear compartment. These findings provide valuable insights into complex regulating human stem niche.

Language: Английский

Citations

4