Therapeutic Potential and Challenges of Mesenchymal Stem Cell-Derived Exosomes for Peripheral Nerve Regeneration: A Systematic Review DOI Open Access
Clelia Dogny, Dominik André‐Lévigne, Daniel F. Kalbermatten

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6489 - 6489

Published: June 12, 2024

Gap injuries to the peripheral nervous system result in pain and loss of function, without any particularly effective therapeutic options. Within this context, mesenchymal stem cell (MSC)-derived exosomes have emerged as a potential option. Thus, focus study was review currently available data on MSC-derived exosome-mounted scaffolds nerve regeneration order identify most promising exosome sources field regeneration. We conducted systematic following PRISMA 2020 guidelines. Exosome origins varied (adipose-derived MSCs, bone marrow gingival MSC, induced pluripotent cells purified product) similarly materials (Matrigel, alginate silicone, acellular graft [ANG], chitosan, chitin, hydrogel fibrin glue). The compound muscle action (CMAP), sciatic functional index (SFI), gastrocnemius wet weight histological analyses were used main outcome measures. Overall, showed better than alone. Functionally, both exosome-enriched chitin ANG significant improvement over time sciatica index, CMAP weight. best outcomes found scaffold with high increase axonal diameter cross-section area. Further studies are needed confirm efficacy

Language: Английский

Small extracellular vesicles purification and scale-up DOI Creative Commons

Xinya Zheng,

Hongru Ai,

Kewen Qian

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 26, 2024

Exosomes are small extracellular vesicles (sEVs) secreted by cells. With advances in the study of sEVs, they have shown great potential diagnosis and treatment disease. However, sEV therapy usually requires a certain dose purity sEVs to achieve therapeutic effect, but existing purification technology exists form low yield, purity, time-consuming, complex operation many other problems, which greatly limits application sEVs. Therefore, how obtain high-purity high-quality quickly efficiently, make them realize large-scale production is major problem current research. This paper discusses improve yield from whole process including upstream cell line selection culture process, downstream isolation purification, quality testing final storage technology.

Language: Английский

Citations

9
Dental pulp regeneration strategies: A review of status quo and recent advances DOI Creative Commons
Xinlu Li, Wei Fan, Bing Fan

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 258 - 275

Published: May 7, 2024

Microorganisms, physical factors such as temperature or mechanical injury, and chemical free monomers from composite resin are the main causes of dental pulp diseases. Current clinical treatment methods for diseases include root canal therapy, vital therapy regenerative endodontic therapy. Regenerative serves purpose inducing regeneration new functional tissues through autologous revascularization tissue engineering. This article first discusses current reviews strategies well research outcomes regarding regeneration. Then in vivo models, prospects challenges were further discussed.

Language: Английский

Citations

9
Magnetically Controlled Strategies for Enhanced Tissue Vascularization DOI

Shilu Zhu,

Liang Xu,

Yang Zhang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(34)

Published: May 14, 2024

Abstract Tissue vascularization plays a critical role in the regeneration and repair of damaged tissues. However, certain instances tissue injury, pace effectiveness can be limited. Innovative strategies leveraging magnetic fields nanoparticles (MNPs) are devised to enhance efficacy vascularization. This review explores potential field‐assisted augmenting repair. Direct application static or dynamic fields, alone combination with MNPs, offers means modulate cellular behaviors gene expression, thereby promoting angiogenesis regeneration. Techniques such as cell labeling, delivery using targeting have shown promise efficiently repairing various ischemic injuries by enhancing These broad applications bone skin regeneration, limb ischemia treatment, myocardial injury diabetic wound therapy. By summarizing recent advancements magnetically controlled strategies, this aims shed light on their future prospects clinical treatment.

Language: Английский

Citations

8
Netrin-1–engineered endothelial cell exosomes induce the formation of pre-regenerative niche to accelerate peripheral nerve repair DOI Creative Commons
Jinsheng Huang, Jiangnan Li, Sen-Rui Li

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(26)

Published: June 28, 2024

The formation of vascular niche is pivotal during the early stage peripheral nerve regeneration. Nevertheless, mechanisms in regulation repair remain unclear. Netrin-1 (NTN1) was found up-regulated stump after injury (PNI). Herein, we demonstrated that NTN1-high endothelial cells (NTN1+ECs) were critical component niche, fostering angiogenesis, axon regeneration, and repair-related phenotypes. We also NTN1+EC–derived exosomes (NTN1 EC-EXO) involved as a role. Multi-omics analysis further verified NTN1 EC-EXO carried low-level expression let7a-5p activated key pathways associated with including focal adhesion, guidance, phosphatidylinositol 3-kinase–AKT, mammalian target rapamycin signaling pathway. Together, our study suggested construction pre-regenerative induced by could establish beneficial microenvironment for facilitate functional recovery PNI.

Language: Английский

Citations

8
PEDOT‐Integrated Fish Swim Bladders as Conductive Nerve Conduits DOI Creative Commons
Hui Zhang, Dongyu Xu, Bin Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 17, 2024

Abstract Advanced artificial nerve conduits offer a promising alternative for injury repair. Current research focuses on improving the therapeutic effectiveness of by optimizing scaffold materials and functional components. In this study, novel poly(3,4‐ethylenedioxythiophene) (PEDOT)‐integrated fish swim bladder (FSB) is presented as conductive conduit with ordered topology electrical stimulation to promote regeneration. PEDOT nanomaterials adhesive peptides (IKVAV) are successfully incorporated onto decellularized FSB substrate through pre‐coating polydopamine. The obtained PEDOT/IKVAV‐integrated exhibits outstanding mechanical properties, high conductivity, stability, well excellent biocompatibility bioadhesive properties. vitro studies confirm that can effectively facilitate growth directional extension pheochromocytoma 12 cells dorsal root ganglion neurites. addition, in vivo experiments demonstrate proposed accelerate defective repair restoration. findings indicate FSB‐derived multiple regenerative inducing signals integration provide conducive milieu regeneration, exhibiting great potential repairing long‐segment neural defects.

Language: Английский

Citations

6
Nerve Regeneration Potential of Antioxidant-Modified Black Phosphorus Quantum Dots in Peripheral Nerve Injury DOI
Junjie Shen, Yi Sun,

Xuanzhe Liu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(34), P. 23518 - 23536

Published: Aug. 16, 2024

Peripheral nerve injury is a major societal concern. Black phosphorus (BP) has inherent advantages over cell-based therapies in regenerative medicine. However, controlling spontaneous degradation and size-dependent cytotoxicity remains challenging poses difficulties for clinical translation. In this study, we constructed zero-dimensional BP quantum dots (QDs) modified with antioxidant β-carotene comprehensively investigated them Schwann cells (SCs) to elucidate their potential peripheral repair. vitro experiments demonstrated that BPQD@β-carotene an inappreciable toxicity good biocompatibility, favoring neural regrowth, angiogenesis, inflammatory regulation of SCs. Furthermore, the PI3K/Akt Ras/ERK1/2 signaling pathways were activated SCs at genetic, protein, metabolite levels. The BPQD@β-carotene-embedded GelMA/PEGDA scaffold enhanced functional recovery by promoting axon remyelination regeneration facilitating intraneural angiogenesis models rats beagle dogs. These results contribute advancing knowledge nanomaterials tissue show significant application translational

Language: Английский

Citations

4
Nanoparticle Strategies for Treating CNS Disorders: A Comprehensive Review of Drug Delivery and Theranostic Applications DOI Open Access
Corneliu Toader, Adrian Dumitru,

Lucian Eva

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13302 - 13302

Published: Dec. 11, 2024

This review aims to address the significant challenges of treating central nervous system (CNS) disorders such as neurodegenerative diseases, strokes, spinal cord injuries, and brain tumors. These are difficult manage due complexity disease mechanisms protective blood–brain barrier (BBB), which restricts drug delivery. Recent advancements in nanoparticle (NP) technologies offer promising solutions, with potential applications delivery, neuroprotection, neuroregeneration. By examining current research, we explore how NPs can cross BBB, deliver medications directly targeted CNS regions, enhance both diagnostics treatment. Key NP strategies, passive targeting, receptor-mediated transport, stimuli-responsive systems, demonstrate encouraging results. Studies show that may improve minimize side effects, increase therapeutic effectiveness models Alzheimer’s, Parkinson’s, stroke, glioblastoma. thus represent a approach for disorder management, combining delivery diagnostic capabilities enable more precise effective treatments could significantly benefit patient outcomes.

Language: Английский

Citations

4
Bio‐Based Elastomers: Design, Properties, and Biomedical Applications DOI Open Access
Qingsheng Liu, Pengfei Lou, Zhentao Sun

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract To reduce carbon footprint and human dependence on fossil fuels, the field of bio‐based polymers has undergone explosive growth in recent years. Among them, elastomers have gained tremendous attention for their inherent softness, high strain, resilience. In this review, progress representative derived from molecular building blocks biopolymers are recapitulated, with an emphasis design, synthesis approaches, mechanical performance. The performance‐advantaged properties elastomers, including immune modulation, biocompatibility, biodegradability also explored. Furthermore, biomedical applications wound dressing, cardiovascular, nerve repair, bone biosensors exemplified. Lastly, challenges outlooks development discussed. This review aims to offer readers valuable insights into potential as viable alternatives petroleum‐based counterparts, supporting transition toward a more sustainable future.

Language: Английский

Citations

0
Metabolic Reprogramming of Neural Stem Cells by Chiral Nanofiber for Spinal Cord Injury DOI Creative Commons
Haining Wu, Chao Xing, Beibei Yu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Exogenous neural stem cells (NSCs) have great potential to reconstitute damage spinal circuitry. However, regulating the metabolic reprogramming of NSCs for reliable nerve regeneration has been challenging. This report discusses biomimetic dextral hydrogel (DH) with right-handed nanofibers that specifically reprograms lipid metabolism NSCs, promoting their differentiation and rapid damaged axons. The underlying mechanism is intrinsic stereoselectivity between DH fatty acid-binding protein 5 (FABP5), which facilitates transportation acids bound FABP5 into mitochondria endoplasmic reticulum, subsequently augmenting acid oxidation (FAO) levels enriching sphingosine biosynthesis. In rat SCI model, significantly improved Basso–Beattie–Bresnahan (BBB) locomotor scores (over 3-fold) hindlimbs' compound muscle action 4-fold) compared untreated group, conveying a significant return functional recovery. finding nanoscale chirality-dependent provides insights understanding cell physiology presents opportunities regenerative medicine.

Language: Английский

Citations

0
Engineered Extracellular Vesicles from Antler Blastema Progenitor Cells: A Therapeutic Choice for Spinal Cord Injury DOI Creative Commons

Shijie Yang,

Borui Xue,

Yongfeng Zhang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Deer antler blastema progenitor cells (ABPCs) are promising for regenerative medicine due to their role in annual regeneration, the only case of complete organ regeneration mammals. ABPC-derived signals show great potential promoting tissues with limited natural ability. Our findings demonstrate capability extracellular vesicles from ABPCs (EVsABPC) repair spinal cord injury (SCI), a condition low capacity. EVsABPC significantly enhanced proliferation neural stem (NSCs) and activated neuronal potential, resulting 5.2-fold increase axonal length. Additionally, exhibited immunomodulatory effects, shifting macrophages M1 M2. Engineered cell-penetrating peptides (ACPPs), outperformed EVs rat bone marrow (EVsBMSC) (EVsNSC), 1.3-fold growth, 30.6% reduction apoptosis, 2.6-fold improvement motor function recovery. These support as therapeutic candidate SCI repair.

Language: Английский

Citations

0