Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation DOI Creative Commons
Jia‐Geng Liu, L. Prahl, Aria Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Engineering of embryonic strategies for tissue-building has extraordinary promise regenerative medicine. This led to a resurgence in interest the relationship between cell biophysical properties and morphological transitions. However, mapping gene or protein expression data physical morphogenesis remains challenging with current techniques. Here we present MATCHY (multiplexed adhesion traction cells at high yield). advances multiplexing throughput capabilities existing force cell-cell assays using microfabrication an automated computation scheme machine learning-driven segmentation. Both are coupled serial downstream immunofluorescence extract type/signaling state information. is especially suited complex primary tissue-, organoid-, biopsy-derived mixtures since it does not rely on

Language: Английский

Advancing Synthetic Hydrogels through Nature‐Inspired Materials Chemistry DOI Creative Commons
Bram G. Soliman,

Ashley K. Nguyen,

J. Justin Gooding

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Synthetic extracellular matrix (ECM) mimics that can recapitulate the complex biochemical and mechanical nature of native tissues are needed for advanced models development disease. Biomedical research has heavily relied on use animal-derived biomaterials, which is now impeding their translational potential convoluting biological insights gleaned from in vitro tissue models. Natural hydrogels have long served as a convenient effective cell culture tool, but advances materials chemistry fabrication techniques present promising new avenues creating xenogenic-free ECM substitutes appropriate organotypic microphysiological systems. However, significant challenges remain synthetic matrices approximate structural sophistication, complexity, dynamic functionality tissues. This review summarizes key properties ECM, discusses recent approaches used to systematically decouple tune these matrices. The importance mechanics, such viscoelasticity plasticity, also discussed, particularly within context organoid engineered Emerging design strategies mimic reviewed, multi-network hydrogels, supramolecular chemistry, assembled monomers.

Language: Английский

Citations

15
Cation‐Alginate Complexes and Their Hydrogels: A Powerful Toolkit for the Development of Next‐Generation Sustainable Functional Materials DOI Creative Commons
Pietro Tordi, Francesca Ridi, Paolo Samorı́

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract The use of materials from renewable sources instead fossil fuels is a crucial step forward in the industrial transition toward sustainability. Among polysaccharides, alginate stands out as versatile and eco‐friendly candidate due to its ability form functional complexes with cations. This review provides an up‐to‐date comprehensive description complexation specific cations, focusing on how interaction forces can be harnessed tailor physicochemical properties cation‐alginate‐based materials. Methodologies approaches for development multiscale characterization these are introduced discussed. Alginate mono‐, di‐, tri‐, tetravalent cations (namely Ag + , Mg 2+ Ca Sr Ba Mn Co Ni Cu Zn Cd Pb UO 2 Cr 3+ Fe Al Ga Y La Ce Nd Eu Tb Gd Zr 4+ Th ) reviewed. Each cation discussed individually, highlighting it uniquely influence material thereby unlocking new potentials design advanced Key challenges opportunities applying across diverse fields, such biomedicine, environmental remediation, food additives supplements, flame retardants, sensors, supercapacitors, catalysis, mechanical isolators assessed, providing evidence transformative potential cation‐alginate tackling global advancing cutting‐edge technologies.

Language: Английский

Citations

13
Surface‐Functionalized Microgels as Artificial Antigen‐Presenting Cells to Regulate Expansion of T Cells DOI
Junzhe Lou, Charlotte Meyer, Einat B. Vitner

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(31)

Published: April 14, 2024

Artificial antigen-presenting cells (aAPCs) are currently used to manufacture T for adoptive therapy in cancer treatment, but a readily tunable and modular system can enable both rapid cell expansion control over phenotype. Here, it is shown that microgels with tailored surface biochemical properties serve as aAPCs mediate activation expansion. Surface functionalization of achieved via layer-by-layer coating using oppositely charged polymers, forming thin dense polymer layer on the surface. This facile versatile approach compatible variety polymers allows efficient flexible surface-specific conjugation defined peptides or proteins. The authors demonstrate tethering appropriate stimulatory ligands microgel efficiently activates polyclonal antigen-specific expansion, phenotype, functional outcome primary mouse human be regulated by modulating concentration, ratio, distribution presented surfaces well stiffness viscoelasticity microgels.

Language: Английский

Citations

6
Hormonally and chemically defined expansion conditions for organoids of biliary tree Stem Cells DOI Creative Commons
Wencheng Zhang, Yangyang Cui,

Mengqi Lu

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 41, P. 672 - 695

Published: Sept. 10, 2024

Language: Английский

Citations

6
Tuning porosity of macroporous hydrogels enables rapid rates of stress relaxation and promotes cell expansion and migration DOI Creative Commons
Bryan A. Nerger,

K. T. Kashyap,

Brendan T. Deveney

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(45)

Published: Oct. 28, 2024

Extracellular matrix (ECM) viscoelasticity broadly regulates cell behavior. While hydrogels can approximate the of native ECM, it remains challenging to recapitulate rapid stress relaxation observed in many tissues without limiting mechanical stability hydrogel. Here, we develop macroporous alginate that have an order magnitude increase rate as compared bulk hydrogels. The increased occurs across a wide range polymer molecular weights (MWs), which enables use high MW for improved depends on volume fraction pores and concentration bovine serum albumin, is added stabilize structure during gelation. Relative spheroids encapsulated hydrogels, significantly larger area smaller circularity because migration. A computational model provides framework relationship between architecture morphogenesis consistent with experimental observations. Taken together, these findings elucidate hydrogel help inform design materials-based therapies.

Language: Английский

Citations

6
Standardization and consensus in the development and application of bone organoids DOI Creative Commons
Wang Jian, Xiao Chen, Ruiyang Li

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 15(2), P. 682 - 706

Published: Dec. 2, 2024

Organoids, self-organized structures derived from stem cells cultured in a specific three-dimensional (3D) vitro microenvironment, have emerged as innovative platforms that closely mimic vivo cellular behavior, tissue architecture, and organ function. Bone organoids, frontier organoid research, can replicate the complex functional characteristics of bone tissue. Recent advancements led to successful development including models callus, woven bone, cartilage, trabecular marrow. These organoids are widely utilized establishing bone-related disease models, injury repair, drug screening. However, significant discrepancies remain between current human skeletal tissues terms morphology functionality, limiting their ability accurately model physiology pathology. To address these challenges promote standardization construction, evaluation, application we convened experts research teams with substantial expertise field. By integrating existing findings, this consortium aims establish consensus guide future organoids.

Language: Английский

Citations

5
Organoid bioprinting: from cells to functional tissues DOI
Michelle S. Huang, Fotis Christakopoulos, Julien G. Roth

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Language: Английский

Citations

5
Co‐Assembled Supramolecular Hydrogelators Enhance Glomerulogenesis in Kidney Organoids Through Cell‐Adhesive Motifs DOI Creative Commons
Johnick F. van Sprang,

Jasper G. M. Aarts,

Martin G. T. A. Rutten

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Abstract Soluble biochemical agents are being employed to generate kidney organoids from human‐induced pluripotent stem cells. However, this soluble factor approach does not consider the effect of mechanical environment on lineage commitment. Here, a mechanoresponsive nano‐environment with cell‐adhesive properties composed supramolecular hydrogelators that co‐assemble into fibrous superstructures form transient network is presented, which used encapsulate organoids. The delayed sol‐gel transition enables diffuse densely packed extracellular organoid space during encapsulation procedure. This allows matrix induce biological response beyond organoid‐hydrogel border, and tune glomerulogenesis inside In manner, biomaterials as complementary tool in tuning commitment refining maturation.

Language: Английский

Citations

4
Mechanical feedback links cell division and dynamics in growing cell collectives DOI
Sumit Sinha, Xin Li, Abdul N. Malmi-Kakkada

et al.

Soft Matter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Local stresses in a tissue, collective property, links cell division and dynamics.

Language: Английский

Citations

0
Viscoelastic Mechanics: From Pathology and Cell Fate to Tissue Regeneration Biomaterial Development DOI
Xinyu Dai, Dan Wu, Ke Xu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Viscoelasticity is the mechanical feature of living tissues and cellular extracellular matrix (ECM) has been recognized as an essential biophysical cue in cell function fate regulation, tissue development homeostasis maintenance, disease progression. These findings provide new insights for biomaterials with comparable viscoelastic properties native ECMs matrix, displaying promising applications regeneration medicine. In this review, relationship between viscoelasticity functions (e.g., regeneration) physiological conditions progression aging, degenerative, fibrosis, tumor) pathological will be especially highlighted to figure out potential therapeutic target treatment inspiration related biomaterial development. Furthermore, understanding response ECM mechanism behind it are comprehensively summarized a pathophysiological basis design. The advances on defect repair also reviewed, suggesting significance matchable microenvironment regeneration. Although challenging, tunable that match show great promise. They could promote regeneration, treat degenerative diseases, support organoids artificial organs.

Language: Английский

Citations

0