Droplets,
tiny
liquid
compartments,
are
increasingly
emerging
in
the
biomedical
and
biomanufacturing
fields
due
to
their
unique
properties
serve
as
templates
or
independent
reaction
units.
Currently,
straightforward
efficient
generation
of
various
functional
droplets
a
biofriendly
manner
remains
challenging.
Herein,
novel
microfluidic-assisted
pneumatic
strategy
is
described
for
customizable
high-throughput
production
monodispersed
droplets,
droplet
size
can
be
precisely
controlled
via
simplified
gas
pressure
regulation
module.
In
particular,
numerous
uniform
alginate
microcarriers
rapidly
fabricated
an
all-aqueous
manner,
wherein
encapsulated
islet
liver
cells
exhibit
favorable
viability
biological
functions.
Furthermore,
by
changing
microchannel
configuration,
several
fluid
manipulation
functions
developed
microfluidic
technology,
such
mixing
laminar
flow,
successfully
incorporated
into
this
platform.
The
generators
with
scalable
functionality
demonstrated
many
scenarios,
including
on-demand
distribution
cell-mimetic
particles,
continuous
synthesis
metal-organic
framework
(MOF),
controllable
preparation
compartmental
microgel,
etc.
These
may
provide
sustainable
inspiration
developing
applications
tissue
organ
engineering,
biomaterials
design,
bioprinting
nozzles,
other
fields.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 2, 2025
Abstract
Porous
nanofibrous
microspheres
(PNMs)
present
a
versatile
and
minimally
invasive
strategy
for
tissue
regeneration,
combining
biomimetic
morphology,
tunable
structure,
injectability.
While
self‐assembly
co‐axial
electrospray
are
explored
PNM
fabrication,
these
methods
limited
in
compositional
versatility
production
scalability.
Here,
3D‐printed
microfluidic
platform
is
presented
that
enables
large‐scale
fabrication
of
PNMs
with
precise
control
over
size,
pore
architecture,
morphology.
can
be
functionalized
bioactive
molecules
through
UV
crosslinking,
enhancing
their
regenerative
potential
by
promoting
osteogenesis
human
bone
marrow
stromal
cells
(hBMSCs),
angiogenesis
umbilical
vein
endothelial
(HUVECs),
exerting
anti‐inflammatory
effects
on
macrophages.
Subcutaneous
implantation
rats
demonstrates
support
cell
infiltration,
minimize
fibrosis,
facilitate
integration,
achieving
complete
penetration
incorporation
within
14
days.
These
findings
establish
as
versatile,
scalable,
customizable
platforms,
ideal
applications
injectable
drugs
or
carriers,
well
powders,
offering
promising
solutions
wound
healing
regeneration.
Macromolecular Rapid Communications,
Journal Year:
2024,
Volume and Issue:
45(11)
Published: March 10, 2024
The
porous
structure
of
microgels
significantly
influences
their
properties
and,
thus,
suitability
for
various
applications,
in
particular
as
building
blocks
tissue
scaffolds.
Porosity
is
one
the
crucial
features
microgel-cell
interactions
and
increases
cells'
accumulation
proliferation.
Consequently,
tailoring
porosity
an
effortless
way
important
but
still
challenging,
especially
nonspherical
microgels.
This
work
presents
a
straightforward
procedure
to
fabricate
complex-shaped
poly(N-isopropyl
acrylamide)
(PNIPAM)
with
tuned
structures
using
so-called
cononsolvency
effect
during
microgel
polymerization.
Therefore,
classical
solvent
reaction
solution
exchanged
from
water
water-methanol
mixtures
stop-flow
lithography
process.
For
cylindrical
higher
methanol
content
fabrication,
greater
degree
collapsing
observed,
aspect
ratio
increases.
Furthermore,
swelling
velocities
change
content,
indicating
modified
structure,
which
confirmed
by
electron
microscopy
micrographs.
patterns
variants
occur
cooling,
revealing
thermal
response
highly
heterogeneous
These
results
show
novel
PNIPAM
any
elongated
2D
shape
tailored
thermoresponsiveness
introducing
Advanced Materials Technologies,
Journal Year:
2024,
Volume and Issue:
9(12)
Published: April 17, 2024
Abstract
For
the
construction
of
accurate
3D
tissue
models
in
vitro,
coculture
model
formed
by
spatio‐controlled
distribution
multiple
cells
is
essential.
Despite
benefits
porous
microspheres
with
low
density,
large
surface
area,
and
high
permeability,
exact
fails
to
appear
using
as
carriers.
Herein,
a
facile
method
proposed
construct
heterogeneous
cell
spatio‐tailored
GelMA
customizable
porosity
structure
size
based
on
droplet
microfluidics
combination
placeholders.
ATPS
emulsions,
containing
two
biocompatible
immiscible
aqueous
phases
cell/Alg
/dextran
(Dex)
mixture
polyethylene
glycol
(PEG)
solution,
are
used
templates
generate
The
micron‐sized
prepared
UV
crosslinking
droplets
microfluidic
devices,
followed
chelation
As
proof
concept,
hepatocellular
carcinoma
(HCC)
HepG2
encapsulated
outside
pores
HUVEC
inside
established.
Noteworthy,
HCC
exhibit
greater
activity
proliferation,
enhanced
functionalities,
drug
resistance
than
control
group.
In
conclusion,
this
straightforward
provides
novel
approach
for
constructing
vitro.
Small,
Journal Year:
2023,
Volume and Issue:
19(38)
Published: May 24, 2023
In
this
study,
a
one-step
method
is
discussed
for
producing
uniform
cell-sized
microgels
using
glass
capillaries
filled
with
binary
polymer
blend
of
polyethylene
glycol
(PEG)
and
gelatin.
Upon
decreasing
temperature,
phase
separation
the
PEG/gelatin
blends
gelation
gelatin
occur,
then
forms
linearly
aligned,
uniformly
sized
in
capillary.
When
DNA
added
to
solution,
entrapping
are
spontaneously
formed,
prevents
coalescence
microdroplets
even
at
temperatures
above
melting
point.
This
novel
form
may
be
applicable
other
biopolymers.
expected
contribute
diverse
materials
science
via
biopolymer
biophysics
synthetic
biology
through
cellular
models
containing
gels.
Frontiers in Chemistry,
Journal Year:
2024,
Volume and Issue:
12
Published: May 13, 2024
In
droplet
microfluidics,
UV-Vis
absorption
spectroscopy
along
with
colorimetric
assays
have
been
widely
used
for
chemical
and
biochemical
analysis.
However,
the
sensitivity
of
measurement
can
be
limited
by
short
optical
pathlength.
Here
we
report
a
novel
design
to
enhance
removing
oil
converting
droplets
into
single-phase
aqueous
flow,
which
measured
within
U-shape
channel
long
The
flow
cells
were
fabricated
via
3D
printing.
calibration
results
demonstrated
complete
removal
effective
pathlengths
similar
designed
lengths
(from
5
20
mm).
cell
was
further
employed
in
microfluidic-based
phosphate
sensing
system.
levels
displayed
excellent
consistency
data
obtained
from
traditional
UV
This
overcomes
limitations
microfluidics
has
potential
situ
continuous
monitoring.
ACS Omega,
Journal Year:
2023,
Volume and Issue:
8(32), P. 29758 - 29769
Published: Aug. 1, 2023
Micromixers
offer
the
advantage
of
rapid
and
homogeneous
mixing
compared
with
conventional
macroscale
reaction
systems,
thus
they
show
great
potential
for
synthesis
nanoparticles.
An
ellipse
curve
serpentine
micromixer,
which
had
been
proposed
in
our
prior
works
was
employed
to
synthesize
Cu2O
are
excellent
photocatalysts
that
have
widely
utilized
degradation
organic
dyes.
Owing
performance,
reduction
Cu(OH)2
micromixing
more
sufficient
than
stirring
synthesis.
The
nanoparticles
synthesized
by
smaller
size
narrower
distribution
those
a
beaker.
smallest
were
obtained
Re
=
100
at
T
60
°C,
while
most
uniform
80
°C
owing
Ostwald
ripening.
Through
photocatalytic
experiments
Rhodamine
B,
found
better
photocatalysis
stirring.
research
results
showed
suitable
choice
produce
photocatalysis.
micromixer
simple
structure
high
performance
can
be
applied
various
