PLLA Porous Scaffold as a 3D Breast Cancer Model to Investigate Drug Resistance DOI

Camilla Carbone,

Salvatrice Rigogliuso, Valerio Brucato

et al.

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

ABSTRACT Multidrug resistance remains one of the major challenges in breast cancer research, often leading to treatment failure. To better understand this mechanism, sophisticated three‐dimensional (3D) tumor models are necessary, as they offer several advantages over traditional bidimensional (2D) cultures. In study, poly‐ l ‐lactic‐acid porous scaffolds were produced using a thermally induced phase separation technique and employed 3D for cell lines: MDA‐MB‐231, MCF‐7, its multidrug‐resistant variant, MCF‐7R. The MTS assay was used compare growth inhibition following doxorubicin 2D 3D. Remarkably, IC 50 values increased cultures compared 2D: MDA‐MB‐231 (445 vs. 54.5 ng/mL), MCF‐7 (7.45 0.75 μg/mL), MCF‐7R (165 39 μg/mL). MCF‐7R, which usually shows greater 2D, demonstrated even higher fact, not reached within 3 days with other models, but only after 6 days. Cellular morphology also played crucial role. When treated concentrations than , cells lost their physiological clustered structure, while resistant variant exhibited disrupted layers. All lines showed chemoresistance, suggesting more biomimetic spatial architecture. Our work bridges gap between monolayer animal highlighting potential polymeric research.

Language: Английский

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases DOI Creative Commons
Nora Bloise, Marialaura Giannaccari, G Guagliano

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 1054 - 1054

Published: June 18, 2024

Over the past decade, development of three-dimensional (3D) models has increased exponentially, facilitating unravelling fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues organs respond to biochemical biophysical stimuli under both physiological pathological conditions. This section presents a concise overview most recent updates on significant contribution different types 3D cell cultures including spheroids, organoids organ-on-chip bio-printed in advancing our understanding molecular mechanisms. The case studies presented include breast cancer (BC), endometriosis, liver microenvironment infections. In BC, establishment culture permitted visualization role cancer-associated fibroblasts delivery exosomes, as well significance physical properties extracellular matrix promoting proliferation invasion. approach also become valuable tool gaining insight general specific drug resistance. Given considerable heterogeneity offer more accurate representation vivo microenvironment, thereby identification translation novel targeted therapeutic strategies. advantages provided hepatic environment, conjunction high throughput characterizing various platforms, have enabled elucidation complex underlying threatening diseases. A limited number for gut skin infections been developed. However, profound comprehension spatial temporal interactions between microbes, host their environment may facilitate advancement vitro, ex disease models. Additionally, it pave way approaches diverse research fields. interested reader will find concluding remarks challenges prospects using discovering areas covered this review.

Language: Английский

Citations

7
Emerging Hydrophobized Surfaces for Rapid Fabrication of 3D Tumor Models DOI
Maria V. Monteiro, Mariana T. Carvalho, Beatriz S. Moura

et al.

ACS Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 961 - 973

Published: Feb. 11, 2025

Language: Английский

Citations

0
Silk fibroin: An innovative protein macromolecule-based hydrogel/scaffold revolutionizing breast cancer treatment and diagnosis – Mechanisms, advancements, and targeting capabilities DOI
Shubhrajit Mantry,

Kotaiah Silakabattini,

Prabhat Kumar Das

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142870 - 142870

Published: April 1, 2025

Language: Английский

Citations

0
Recent advancements and challenges in 3D bioprinting for cancer applications DOI
Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 43, P. e00357 - e00357

Published: Sept. 13, 2024

Language: Английский

Citations

1
PLLA Porous Scaffold as a 3D Breast Cancer Model to Investigate Drug Resistance DOI

Camilla Carbone,

Salvatrice Rigogliuso, Valerio Brucato

et al.

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

ABSTRACT Multidrug resistance remains one of the major challenges in breast cancer research, often leading to treatment failure. To better understand this mechanism, sophisticated three‐dimensional (3D) tumor models are necessary, as they offer several advantages over traditional bidimensional (2D) cultures. In study, poly‐ l ‐lactic‐acid porous scaffolds were produced using a thermally induced phase separation technique and employed 3D for cell lines: MDA‐MB‐231, MCF‐7, its multidrug‐resistant variant, MCF‐7R. The MTS assay was used compare growth inhibition following doxorubicin 2D 3D. Remarkably, IC 50 values increased cultures compared 2D: MDA‐MB‐231 (445 vs. 54.5 ng/mL), MCF‐7 (7.45 0.75 μg/mL), MCF‐7R (165 39 μg/mL). MCF‐7R, which usually shows greater 2D, demonstrated even higher fact, not reached within 3 days with other models, but only after 6 days. Cellular morphology also played crucial role. When treated concentrations than , cells lost their physiological clustered structure, while resistant variant exhibited disrupted layers. All lines showed chemoresistance, suggesting more biomimetic spatial architecture. Our work bridges gap between monolayer animal highlighting potential polymeric research.

Language: Английский

Citations

1