Nano Research, Journal Year: 2022, Volume and Issue: 16(1), P. 905 - 916
Published: Aug. 17, 2022
Language: Английский
Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522
Published: Dec. 21, 2022
Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.
Language: Английский
Citations
270
Advanced Science, Journal Year: 2022, Volume and Issue: 9(13)
Published: March 6, 2022
Electroconductive hydrogels are very attractive candidates for accelerated spinal cord injury (SCI) repair because they match the electrical and mechanical properties of neural tissue. However, electroconductive hydrogel implantation can potentially aggravate inflammation, hinder its efficacy. Bone marrow stem cell-derived exosomes (BMSC-exosomes) have shown immunomodulatory tissue regeneration effects, therefore, tissue-like loaded with BMSC-exosomes developed synergistic treatment SCI. These exosomes-loaded modulate microglial M2 polarization via NF-κB pathway, synergistically enhance neuronal oligodendrocyte differentiation cells (NSCs) while inhibiting astrocyte differentiation, also increase axon outgrowth PTEN/PI3K/AKT/mTOR pathway. Furthermore, combined significantly decrease number CD68-positive microglia, local NSCs recruitment, promote axonal regeneration, resulting in significant functional recovery at early stage an SCI mouse model. Hence, findings this study demonstrate that combination is a promising therapeutic strategy repair.
Language: Английский
Citations
253
Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 33(6)
Published: Dec. 3, 2022
Abstract Bioadhesives have been used in clinics among the most prospective alternatives to sutures and staples for wound sealing repairing; however, they generally inadequate adhesion wet surfaces, improper mechanical strength, poor hemostasis, cytotoxicity. To address these challenges, a robust tissue adhesive based on collagen starch materials (CoSt) is designed this study. CoSt hydrogels integrate feature of drainage, molecular penetration strengthen cross‐linking similar mussel, ivy, oyster glues, which remove interfacial water quickly, reinforce tough dissipation involve multiple reversible dynamic interactions. Therefore, form strong sutureless injured tissues, accompanying actuate biointerfaces direct contact with liquids or blood, resolving crucial impediments commercially accessible adhesives. The novel bioadhesive shows repeatable adhesiveness (62 ± 4.8 KPa), high performance (153.2 35.1 mmHg), fast self‐healing ability, excellent injectability, shape adaptability. For different hemostatic needs rat models tail amputation, skin incision, severe liver, abdominal aorta, transected nerve injuries, hydrogel better efficiency than fibrin glue because coordinate efficacy property, outstanding red blood cell arresting capability, activation barrier membrane. Moreover, vivo investigation injury repair model validate that accelerate healing functional recovery via damage/defects. Tough adhesion, quick distinguished biocompatibility, suitability match irregular‐shaped target sites, good promotion makes it various biomedical applications.
Language: Английский
Citations
119
Theranostics, Journal Year: 2023, Volume and Issue: 13(6), P. 2015 - 2039
Published: Jan. 1, 2023
Increasing data reveals that gelatin has been methacrylated is involved in a variety of physiologic processes are important for therapeutic interventions. Gelatin methacryloyl (GelMA) hydrogel highly attractive hydrogels-based bioink because its good biocompatibility, low cost, and photo-cross-linking structure useful cell survivability monitoring. Methacrylated established itself as typical composition with extensive biomedical applications. Recent advances GelMA have focused on integrating them bioactive functional nanomaterials, the goal improving GelMA's physical, chemical, biological properties. ability to modify characteristics due synthesis technique also makes it choice soft hard tissues. become an independent or supplementary technology musculoskeletal problems. Here, we systematically review mechanism-of-action, uses, challenges future direction disorders. We give overview nanocomposite different applications disorders, such osteoarthritis, intervertebral disc degeneration, bone regeneration, tendon disorders so on.
Language: Английский
Citations
80
Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 306, P. 120578 - 120578
Published: Jan. 18, 2023
Language: Английский
Citations
69
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(13)
Published: Jan. 28, 2023
Abstract Photothermal hydrogel adhesives have yielded promising results for wound closure and infected treatment in recent years. However, photothermal bioadhesives with on‐demand removability without additional nanomaterials‐based agents rarely been reported the literature. In this work, an injectable intrinsic bioadhesive removal trait is developed through dynamic cross‐linking of gelatin (Gel), tannic acid (TA) quinone, borax closing skin incisions accelerating methicillin‐resistant Staphylococcus aureus (MRSA) healing. The TA quinone containing polyphenol groups multifunctional adhesiveness performance confer adhesive near‐infrared (NIR) responsive antibacterial activity. pH‐sensitive boronic ester (polyphenol−B) Schiff base bonds endow great self‐healing capacity removability. Moreover, possesses good biocompatibility, injectability, hemostasis. vivo experiment a rat cutaneous incision model full‐thickness MRSA‐infected indicate that smart can close wounds efficiently treat ones, demonstrating its superiority noninvasive enhancing
Language: Английский
Citations
63
Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)
Published: April 20, 2023
Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.
Language: Английский
Citations
62
ACS Nano, Journal Year: 2023, Volume and Issue: 17(9), P. 8530 - 8550
Published: April 28, 2023
Exosomes (EXs) shed by mesenchymal stem cells (MSCs) are potent therapeutic agents that promote wound healing and regeneration, but when used alone in vivo, their potency is diminished rapid clearance bioactivity loss. Inspired the biotin-avidin interaction, we developed a simple yet versatile method for immobilization of MSC-derived EXs (MSC-EXs) into hydrogels achieved sustained release regenerative purposes. First, biotin-modified gelatin methacryloyl (Bio-GelMA) was fabricated grafting NHS-PEG12-biotin onto amino groups GelMA. Biotin-modified MSC-EXs (Bio-EXs) were then synthesized using an situ self-assembling biotinylation strategy, which provided sufficient binding sites MSC-EX delivery with little effect on cargo composition. Thereafter, Bio-EXs immobilized Bio-GelMA via streptavidin to generate Bio-GelMA@Bio-EX hydrogels. An vitro analysis demonstrated could be taken up macrophages exerted immunomodulatory effects similar those MSC-EXs, 7 days. After subcutaneous transplantation, more constant retention observed 28 When placed artificial periodontal multitissue defect, functionalized exhibited optimized performance regrow complex tissues, including acellular cementum, ligaments (PDLs), alveolar bone. In this context, robust promoted macrophage polarization toward M2 phenotype. Our findings demonstrate delivered aid system exhibit macrophage-modulating repair-promoting functions suggest universal approach development MSC-EX-functionalized biomaterials advanced therapies.
Language: Английский
Citations
58
Protein & Cell, Journal Year: 2023, Volume and Issue: 14(9), P. 635 - 652
Published: Feb. 10, 2023
Abstract Spinal cord injury (SCI) disrupts the structural and functional connectivity between higher center spinal cord, resulting in severe motor, sensory, autonomic dysfunction with a variety of complications. The pathophysiology SCI is complicated multifaceted, thus individual treatments acting on specific aspect or process are inadequate to elicit neuronal regeneration recovery after SCI. Combinatory strategies targeting multiple aspects pathology have achieved greater beneficial effects than therapy alone. Although many problems challenges remain, encouraging outcomes that been preclinical models offer promising foothold for development novel clinical treat In this review, we characterize mechanisms underlying axon adult neurons summarize recent advances facilitating following at both acute chronic stages. addition, analyze current status, remaining problems, realistic towards translation. Finally, consider future treatment provide insights into how narrow translational gap currently exists studies practice. Going forward, trials should emphasize multidisciplinary conversation cooperation identify optimal combinatorial approaches maximize therapeutic benefit humans
Language: Английский
Citations
57
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 24, 2023
Spinal cord injury (SCI) has considerable impact on patient physical, mental, and financial health. Secondary SCI is associated with inflammation, vascular destruction, subsequent permanent damage to the nervous system. Mesenchymal stem cells (MSCs) have anti-inflammatory properties, promoting regeneration release neuro-nutrients, are a promising strategy for treatment of SCI. Preclinical studies shown that MSCs promote sensory motor function recovery in rats. In clinical trials, been reported improve American Injury Association (ASIA) scores. However, effectiveness treating patients remains controversial. tumorigenesis ensuring survival hostile environment challenging. this article we examine evidence pathophysiological changes occurring after We then review underlying mechanisms summarize potential application practice. Finally, highlight challenges surrounding use discuss future applications.
Language: Английский
Citations
53