Mechano‐Activated Cell Therapy for Accelerated Diabetic Wound Healing

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(47)

Published: Sept. 8, 2023

Chronic diabetic wounds are a significant global healthcare challenge. Current strategies, such as biomaterials, cell therapies, and medical devices, however, only target few pathological features have limited efficacy. A powerful platform technology combining magneto-responsive hydrogel, cells, wireless magneto-induced dynamic mechanical stimulation (MDMS) is developed to accelerate wound healing. The hydrogel encapsulates U.S. Food Drug Administration (FDA)-approved fibroblasts keratinocytes achieve ∼3-fold better closure in mouse model. MDMS acts nongenetic mechano-rheostat activate fibroblasts, resulting ∼240% proliferation, ∼220% more collagen deposition, improved keratinocyte paracrine profiles via the Ras/MEK/ERK pathway boost angiogenesis. property also enables on-demand insulin release for spatiotemporal glucose regulation through increasing network deformation interstitial flow. By mining scRNAseq data, mechanosensitive fibroblast subpopulation identified that can be mechanically tuned enhanced proliferation production, maximizing therapeutic impact. "all-in-one" system addresses major factors associated with single platform, potential applications other challenging types.

Language: Английский

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Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 10(21)

Published: April 29, 2023

Abstract Cells interact with their surrounding environment through a combination of static and dynamic mechanical signals that vary over stimulus types, intensity, space, time. Compared to such as stiffness, porosity, topography, the current understanding on effects stimulations cells remains limited, attributing lack access devices, complexity experimental set‐up, data interpretation. Yet, in pursuit emerging translational applications (e.g., cell manufacturing for clinical treatment), it is crucial understand how respond variety forces are omnipresent vivo so they can be exploited enhance therapeutic outcomes. With rising appreciation extracellular matrix (ECM) key regulator biofunctions, researchers have bioengineered suite ECM‐mimicking hydrogels, which fine‐tuned spatiotemporal cues model complex profiles. This review first discusses stimuli may impact different cellular components various mechanobiology pathways involved. Then, hydrogels designed incorporate parameters influence behaviors described. The Scopus database also used analyze relative strength evidence, ranging from strong weak, based number published literatures, associated citations, treatment significance. Additionally, impacts clinically relevant types including mesenchymal stem cells, fibroblasts, immune evaluated. aim draw attention paucity studies well highlight potential using cocktail intensities fates (similar concept biochemical direct fate). It envisioned this progress report will inspire more exciting development mechanoresponsive biomedical applications.

Language: Английский

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Optimizing scaffold pore size for tissue engineering: insights across various tissue types

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 12, 2024

Scaffold porosity is a critical factor in replicating the complex vivo microenvironment, directly influencing cellular interactions, migration, nutrient transfer, vascularization, and formation of functional tissues. For optimal tissue formation, scaffold design must account for various parameters, including material composition, morphology, mechanical properties, compatibility. This review highlights importance interconnected pore size, emphasizing their impact on behavior across several engineering domains, such as skin, bone, cardiovascular, lung Specific size ranges enhance functionality different tissues: small pores (∼1–2 µm) aid epidermal cell attachment skin regeneration, moderate (∼2–12 support dermal larger (∼40–100 facilitate vascular structures. bone engineering, multi-layered scaffolds with smaller (50–100 foster attachment, while (200–400 diffusion angiogenesis. Cardiovascular tissues benefit from sizes (∼25–60 to balance integration diffusion. By addressing challenges optimizing distributions, this provides insights into innovations, ultimately advancing regeneration strategies.

Language: Английский

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Injectable self-crosslinking hyaluronic acid/silk fibroin blend hydrogel based on disulfide bond

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: 356, P. 123374 - 123374

Published: Feb. 11, 2025

Language: Английский

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Development of Biocompatible 3D-Printed Artificial Blood Vessels through Multidimensional Approaches

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(10), P. 497 - 497

Published: Oct. 8, 2023

Within the human body, intricate network of blood vessels plays a pivotal role in transporting nutrients and oxygen maintaining homeostasis. Bioprinting is an innovative technology with potential to revolutionize this field by constructing complex multicellular structures. This technique offers advantage depositing individual cells, growth factors, biochemical signals, thereby facilitating functional vessels. Despite challenges fabricating vascularized constructs, bioprinting has emerged as advance organ engineering. The continuous evolution biomaterial knowledge provides avenue overcome hurdles associated tissue fabrication. article overview biofabrication process used create vascular constructs. It delves into various techniques engineering, including extrusion-, droplet-, laser-based methods. Integrating these prospect crafting artificial remarkable precision functionality. Therefore, impact engineering significant. With technological advances, it holds promise revolutionizing transplantation, regenerative medicine. By mimicking natural complexity vessels, brings us one step closer organs vasculature, ushering new era medical advancement.

Language: Английский

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Advances in cancer mechanobiology: Metastasis, mechanics, and materials

APL Bioengineering, Journal Year: 2024, Volume and Issue: 8(1)

Published: March 1, 2024

Within the tumor microenvironment (TME), cells are exposed to numerous mechanical forces, both internally and externally, which contribute metastatic cascade. From initial growth of traveling through vasculature eventual colonization distant organs, continuously interacting with their surroundings physical contact force application. The forces found in TME can be simplified into three main categories: (i) shear stress, (ii) tension strain, (iii) solid stress compression. Each type independently impact progression. Here, we review recent bioengineering strategies, have been employed establish connection between While many cancers explored this review, place great emphasis on that understudied response such as ovarian colorectal cancers. We discuss major steps transformation present novel, advances model systems used study how end by summarizing incorporate multiple expand complexity our understanding sense respond environment. Future studies would also benefit from inclusion time or aspect memory further enhance field. knowledge metastasis grows, developing novel materials vitro essential providing new insight predicting, treating, preventing cancer progression metastasis.

Language: Английский

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Direct fibroblast reprogramming: an emerging strategy for treating organic fibrosis

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 27, 2025

Direct reprogramming has garnered considerable attention due to its capacity directly convert differentiated cells into desired cells. Fibroblasts are frequently employed in studies their abundance and accessibility. However, they also the key drivers progression of fibrosis, a pathological condition characterized by excessive extracellular matrix deposition tissue scarring. Furthermore, initial stage typically involves deactivating fibrotic pathways. Hence, direct offers valuable method regenerate target for repair while simultaneously reducing tendencies. Understanding link between fibrosis could help develop effective strategies treat damaged with potential risk fibrosis. This review summarizes advances reveals anti-fibrosis effects various organs such as heart, liver, skin. we dissect mechanisms influenced molecules including TGF-β signaling, mechanical inflammation epigenetic modifiers, metabolic regulators. Innovative methods fibroblast like small molecules, CRISPRa, modified mRNA, challenges cellular heterogeneity senescence faced vivo reprogramming, discussed.

Language: Английский

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Mechanically programming anisotropy in engineered muscle with actuating extracellular matrices

Device, Journal Year: 2023, Volume and Issue: 1(4), P. 100097 - 100097

Published: Oct. 1, 2023

The hierarchical design and adaptive functionalities of biological tissues are driven by dynamic biochemical, electrical, mechanical signaling between cells their extracellular matrices. While existing tools enable monitoring controlling biochemical electrical in multicellular systems, there is a significant need for techniques that mapping modulating intercellular signaling. We have developed magnetically actuated matrix serves as mechanically active substrate can program morphological functional anisotropy such skeletal muscle. This method improves the ease efficiency programming muscle force directionality synchronicity applications ranging from medicine to robotics. Additionally, we present an open-source computational framework enabling quantitative analyses contractility. Our actuating matrices accompanying broadly applicable across cell types hydrogel chemistries, they drive fundamental studies mechanobiology well translational engineered machines.

Language: Английский

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Magnetically Controlled Strategies for Enhanced Tissue Vascularization

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(34)

Published: May 14, 2024

Abstract Tissue vascularization plays a critical role in the regeneration and repair of damaged tissues. However, certain instances tissue injury, pace effectiveness can be limited. Innovative strategies leveraging magnetic fields nanoparticles (MNPs) are devised to enhance efficacy vascularization. This review explores potential field‐assisted augmenting repair. Direct application static or dynamic fields, alone combination with MNPs, offers means modulate cellular behaviors gene expression, thereby promoting angiogenesis regeneration. Techniques such as cell labeling, delivery using targeting have shown promise efficiently repairing various ischemic injuries by enhancing These broad applications bone skin regeneration, limb ischemia treatment, myocardial injury diabetic wound therapy. By summarizing recent advancements magnetically controlled strategies, this aims shed light on their future prospects clinical treatment.

Language: Английский

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Enhancing Osteoblast Differentiation from Adipose-Derived Stem Cells Using Hydrogels and Photobiomodulation: Overcoming In Vitro Limitations for Osteoporosis Treatment

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(7), P. 6346 - 6365

Published: June 25, 2024

Osteoporosis represents a widespread and debilitating chronic bone condition that is increasingly prevalent globally. Its hallmark features include reduced density heightened fragility, which significantly elevate the risk of fractures due to decreased presence mature osteoblasts. The limitations current pharmaceutical therapies, often accompanied by severe side effects, have spurred researchers seek alternative strategies. Adipose-derived stem cells (ADSCs) hold considerable promise for tissue repair, albeit they encounter obstacles such as replicative senescence in laboratory conditions. In comparison, employing ADSCs within three-dimensional (3D) environments provides an innovative solution, replicating natural extracellular matrix environment while offering controlled cost-effective vitro platform. Moreover, utilization photobiomodulation (PBM) has emerged method enhance ADSC differentiation proliferation potential instigating cellular stimulation facilitating beneficial performance modifications. This literature review critically examines shortcomings osteoporosis treatments investigates synergies between 3D cell culture PBM augmenting towards osteogenic lineages. primary objective this study assess efficacy combined enhancing management. research notably distinguished its thorough scrutiny existing literature, synthesis recent advancements, identification future trajectories, databases PubMed, Scopus, Web Science, Google Scholar review. Furthermore, exploration biomechanical biophysical stimuli holds refining treatment outlook suggests integrating with housed advancing regeneration efforts. Importantly, aspires catalyse further advancements therapeutic strategies regeneration.

Language: Английский

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