Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: April 18, 2024
Degenerative
orthopaedic
diseases
pose
a
notable
worldwide
public
health
issue
attributable
to
the
global
aging
population.
Conventional
medical
approaches,
encompassing
physical
therapy,
pharmaceutical
interventions,
and
surgical
methods,
face
obstacles
in
halting
or
reversing
degenerative
process.
In
recent
times,
exosome-based
therapy
has
gained
widespread
acceptance
popularity
as
an
effective
treatment
for
diseases.
This
therapeutic
approach
holds
potential
"cell-free"
tissue
regeneration.
Exosomes,
membranous
vesicles
resulting
from
fusion
of
intracellular
multivesicles
with
cell
membrane,
are
released
into
extracellular
matrix.
Addressing
challenges
such
rapid
elimination
natural
exosomes
vivo
limitation
drug
concentration
can
be
effectively
achieved
through
various
strategies,
including
engineering
modification,
gene
overexpression
biomaterial
binding.
review
provides
concise
overview
source,
classification,
preparation
methods
exosomes,
followed
by
in-depth
analysis
their
functions
applications.
Furthermore,
explores
strategies
utilizing
diseases,
overexpression,
The
primary
objective
is
provide
fresh
viewpoint
on
utilization
addressing
bone
conditions
support
practical
application
theranosis
Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(26)
Published: July 9, 2023
Abstract
In
clinical
practice,
repairing
osteochondral
defects
presents
a
challenge
due
to
the
varying
biological
properties
of
articular
cartilages
and
subchondral
bones.
Thus,
elucidating
how
spatial
microenvironment‐specific
biomimetic
scaffolds
can
be
used
simultaneously
regenerate
tissue
is
an
important
research
topic.
Herein,
novel
bioinspired
double‐network
hydrogel
scaffold
produced
via
3D
printing
with
tissue‐specific
decellularized
extracellular
matrix
(dECM)
human
adipose
mesenchymal
stem
cell
(MSC)‐derived
exosomes
described.
The
bionic
promote
rat
bone
marrow
MSC
attachment,
spread,
migration,
proliferation,
chondrogenic
osteogenic
differentiation
in
vitro,
as
determined
based
on
sustained
release
bioactive
exosomes.
Furthermore,
3D‐printed
heterogeneous
bilayer
efficiently
accelerate
simultaneous
regeneration
cartilage
tissues
preclinical
model.
conclusion,
dECM‐based
biomimetics
encapsulated
serve
cell‐free
recipe
for
therapy
when
treating
injured
or
degenerative
joints.
This
strategy
provides
promising
platform
complex
zonal
whilst
holding
attractive
translation
potential.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(34)
Published: Sept. 22, 2023
Additive
manufacturing
(AM),
which
is
based
on
the
principle
of
layer-by-layer
shaping
and
stacking
discrete
materials,
has
shown
significant
benefits
in
fabrication
complicated
implants
for
tissue
engineering
(TE).
However,
many
native
tissues
exhibit
anisotropic
heterogenous
constructs
with
diverse
components
functions.
Consequently,
replication
biomimetic
using
conventional
AM
processes
a
single
material
challenging.
Multimaterial
3D
4D
bioprinting
(with
time
as
fourth
dimension)
emerged
promising
solution
constructing
multifunctional
that
can
mimic
host
microenvironment
better
than
single-material
alternatives.
Notably,
4D-printed
multimaterial
architectures
provide
time-dependent
programmable
dynamic
promote
cell
activity
regeneration
response
to
external
stimuli.
This
paper
first
presents
typical
design
strategies
TE
applications.
Subsequently,
latest
are
discussed,
along
their
advantages
challenges.
In
particular,
potential
smart
highlighted.
Furthermore,
this
review
provides
insights
into
how
facilitate
realization
next-generation
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Feb. 10, 2024
Abstract
Extracellular
vesicles
have
shown
promising
tissue
recovery-promoting
effects,
making
them
increasingly
sought-after
for
their
therapeutic
potential
in
wound
treatment.
However,
traditional
extracellular
vesicle
applications
suffer
from
limitations
such
as
rapid
degradation
and
short
maintenance
during
administration.
To
address
these
challenges,
a
growing
body
of
research
highlights
the
role
hydrogels
effective
carriers
sustained
release,
thereby
facilitating
healing.
The
combination
with
development
3D
bioprinting
create
composite
hydrogel
systems
boasting
excellent
mechanical
properties
biological
activity,
presenting
novel
approach
to
healing
skin
dressing.
This
comprehensive
review
explores
remarkable
hydrogels,
specifically
suited
loading
vesicles.
We
delve
into
diverse
sources
analyzing
integration
within
formulations
Different
methods
well
bioprinting,
adapted
varying
conditions
construction
strategies,
are
examined
roles
promoting
results
highlight
vesicle-laden
advanced
tools
field
treatment,
offering
both
support
bioactive
functions.
By
providing
an
in-depth
examination
various
that
can
play
healing,
this
sheds
light
on
directions
further
development.
Finally,
we
challenges
associated
application
along
emerging
trends
domain.
discussion
covers
issues
scalability,
regulatory
considerations,
translation
technology
practical
clinical
settings.
In
conclusion,
underlines
significant
contributions
hydrogel-mediated
therapy
regeneration.
It
serves
valuable
resource
researchers
practitioners
alike,
fostering
deeper
understanding
benefits,
applications,
involved
utilizing
Graphical
abstract
Bioactive Materials,
Journal Year:
2024,
Volume and Issue:
38, P. 1 - 30
Published: April 23, 2024
Characterized
by
their
pivotal
roles
in
cell-to-cell
communication,
cell
proliferation,
and
immune
regulation
during
tissue
repair,
exosomes
have
emerged
as
a
promising
avenue
for
"cell-free
therapy"
clinical
applications.
Hydrogels,
possessing
commendable
biocompatibility,
degradability,
adjustability,
physical
properties
akin
to
biological
tissues,
also
found
extensive
utility
engineering
regenerative
repair.
The
synergistic
combination
of
hydrogels
holds
the
potential
not
only
enhance
efficiency
but
collaboratively
advance
repair
process.
This
review
has
summarized
advancements
made
over
past
decade
research
hydrogel-exosome
systems
regenerating
various
tissues
including
skin,
bone,
cartilage,
nerves
tendons,
with
focus
on
methods
encapsulating
releasing
within
hydrogels.
It
critically
examined
gaps
limitations
current
research,
whilst
proposed
future
directions
applications
this
innovative
approach.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(10), P. 7504 - 7520
Published: Feb. 27, 2024
The
essential
role
of
the
neural
network
in
enhancing
bone
regeneration
has
often
been
overlooked
biomaterial
design,
leading
to
delayed
or
compromised
healing.
Engineered
mesenchymal
stem
cells
(MSCs)-derived
exosomes
are
becoming
increasingly
recognized
as
potent
cell-free
agents
for
manipulating
cellular
behavior
and
improving
therapeutic
effectiveness.
Herein,
MSCs
stimulated
with
nerve
growth
factor
(NGF)
regulate
exosomal
cargoes
improve
neuro-promotive
potential
facilitate
innervated
regeneration.
In
vitro
cell
experiments
showed
that
NGF-stimulated
MSCs-derived
(N-Exos)
obviously
improved
function
neurotrophic
effects
cells,
consequently,
osteogenic
osteo-reparative
cells.
Bioinformatic
analysis
by
miRNA
sequencing
pathway
enrichment
revealed
beneficial
N-Exos
may
partly
be
ascribed
NGF-elicited
multicomponent
miRNAs
subsequent
regulation
activation
MAPK
PI3K-Akt
signaling
pathways.
On
this
basis,
were
delivered
on
micropores
3D-printed
hierarchical
porous
scaffold
accomplish
sustained
release
profile
extended
bioavailability.
a
rat
model
distal
femoral
defect,
N-Exos-functionalized
significantly
induced
neurovascular
structure
formation
This
study
provided
feasible
strategy
modulate
functional
acquire
desirable
potential.
Furthermore,
developed
represent
promising
neurovascular-promotive
reparative
clinical
translation.
Regenerative Biomaterials,
Journal Year:
2023,
Volume and Issue:
11
Published: Dec. 1, 2023
Abstract
Despite
the
considerable
advancements
in
fabricating
polymeric-based
scaffolds
for
tissue
engineering,
clinical
transformation
of
these
remained
a
big
challenge
because
difficulty
simulating
native
organs/tissues’
microenvironment.
As
kind
natural
tissue-derived
biomaterials,
decellularized
extracellular
matrix
(dECM)-based
have
gained
attention
due
to
their
unique
biomimetic
properties,
providing
specific
microenvironment
suitable
promoting
cell
proliferation,
migration,
attachment
and
regulating
differentiation.
The
medical
applications
dECM-based
addressed
critical
challenges,
including
poor
mechanical
strength
insufficient
stability.
For
reconstruction
damaged
tissues
or
organs,
different
types
composite
platforms
been
designed
mimic
microenvironment,
by
integrating
with
polymer
or/and
syntenic
adding
bioactive
factors.
In
this
review,
we
summarized
research
progress
regenerative
medicine,
highlighting
challenges
future
perspectives
related
application
materials.
