Nanomanaging Chronic Wounds with Targeted Exosome Therapeutics DOI Creative Commons
Anita Yadav, Anu Sharma,

Mohini Moulick

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 366 - 366

Published: March 13, 2025

Chronic wounds pose a significant healthcare challenge, impacting millions of patients worldwide and burdening systems substantially. These often occur as comorbidities are prone to infections. Such infections hinder the healing process, complicating clinical management proving recalcitrant therapy. The environment within wound itself poses challenges such lack oxygen, restricted blood flow, oxidative stress, ongoing inflammation, bacterial presence. Traditional systemic treatment for chronic peripheral may not be effective due inadequate supply, resulting in unintended side effects. Furthermore, topical applications impervious persistent biofilm A growing concern is therapeutic modalities treating wounds. Additionally, chemically harsh microenvironment can reduce effectiveness treatments, highlighting need drug delivery that deliver therapies precisely where needed with optimal dosages. Compared cell-based therapies, exosome-based offer distinct advantages cell-free approach treatment. Exosomes endosomal origin enable cell-to-cell communications, they possess benefits, including biocompatibility decreased immunogenicity, making them ideal vehicles efficient targeting minimizing off-target damage. However, exosomes rapidly cleared from body, it difficult maintain concentrations at sites. hydrogel-based development biocompatible scaffolds beneficial sustained release prolong presence these Engineered have been shown stability promoting compared their unmodified counterparts. Significant progress has made this field, but further research essential unlock potential. This review seeks explore benefits opportunities wounds, ensuring efficacy precise despite obstacles posed by environment.

Language: Английский

Adipose‐Derived Mesenchymal Stem Cell‐Derived Exosomes Biopotentiated Extracellular Matrix Hydrogels Accelerate Diabetic Wound Healing and Skin Regeneration DOI Creative Commons
Yanling Song, Yuchan You, Xinyi Xu

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(30)

Published: Sept. 15, 2023

Wound healing is an urgent clinical challenge, particularly in the case of chronic wounds. Traditional approaches to wound have limited therapeutic efficacy due lengthy times, risk immune rejection, and susceptibility infection. Recently, adipose-derived mesenchymal stem cell-derived exosomes (ADSC-exos) emerged as a promising modality for tissue regeneration repair. In this study, development novel extracellular matrix hydrogel@exosomes (ECM@exo) reported, which entails incorporation ADSC-exos into hydrogel (ECM hydrogel). This solution forms at physiological temperature (≈37 °C) upon local injection site. ECM@exo enables sustained release from ECM hydrogel, maintains high concentrations The displays good biocompatibility biodegradability. vivo vitro results demonstrate that treatment effectively reduces inflammation promotes angiogenesis, collagen deposition, cell proliferation, migration, thereby accelerating process. Overall, innovative approach offers new avenue via biological with controlled exosome release.

Language: Английский

Citations

90
Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healing DOI Creative Commons
Xiaoxue Han, Chaimongkol Saengow,

Leah Ju

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 23, 2024

Abstract Wound healing is an obvious clinical concern that can be hindered by inadequate angiogenesis, inflammation, and chronic hypoxia. While exosomes derived from adipose tissue-derived stem cells have shown promise in accelerating carrying therapeutic growth factors microRNAs, intracellular cargo delivery compromised hypoxic tissues due to activated hypoxia-induced endocytic recycling. To address this challenge, we developed a strategy coat oxygen nanobubbles with incorporate them into polyvinyl alcohol/gelatin hybrid hydrogel. This approach not only alleviates wound hypoxia but also offers efficient means of delivering exosome-coated nanoparticles conditions. The self-healing properties the hydrogel, along its component, gelatin, aids hemostasis, while crosslinking bonds facilitate hydrogen peroxide decomposition, ameliorate inflammation. Here, show potential multifunctional hydrogel for enhanced healing, promoting facilitating exosome delivery, mitigating hypoxia, inhibiting inflammation male rat full-thickness model.

Language: Английский

Citations

78
Stem Cell‐Derived Nanovesicles Embedded in Dual‐Layered Hydrogel for Programmed ROS Regulation and Comprehensive Tissue Regeneration in Burn Wound Healing DOI
Meijiao Zhao,

Miaomiao Kang,

Jingru Wang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(32)

Published: June 1, 2024

Abstract Burn wounds often bring high risks of delayed healing process and even death. Reactive oxygen species (ROS) play a crucial role in burn wound repair. However, the dynamic requires both generation ROS to inhibit bacteria subsequent reduction levels initiate promote tissue regeneration, which calls for more intelligent regulation dressing system. Hence, dual‐layered hydrogel (Dual‐Gel) tailored repair is designed: inner layer (Gel 2) first responds bacterial hyaluronidase (Hyal) deliver aggregation‐induced emission photosensitizer functionalized adipose‐derived stem cell nanovesicles, generate upon light irradiation eliminate bacteria; then outer 1) continuously starts long‐lasting consumption excess at site accelerate regeneration. Simultaneously, nanovesicles trapped burns also provide nutrients mobilize neighboring tissues thoroughly assist inflammation regulation, proliferation, migration, angiogenesis. In summary, this study develops an treatment approach on by programmatically regulating facilitating comprehensive

Language: Английский

Citations

71
An ATP-activated spatiotemporally controlled hydrogel prodrug system for treating multidrug-resistant bacteria-infected pressure ulcers DOI
Xiaoliang Qi,

Yajing Xiang,

Ying Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 45, P. 301 - 321

Published: Nov. 27, 2024

Language: Английский

Citations

24
Biointerface‐Engineered Hybrid Nanovesicles for Targeted Reprogramming of Tumor Microenvironment DOI

Xueyan Zhen,

Yongjiang Li,

Wanqing Yuan

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(41)

Published: June 9, 2024

Abstract The tumor microenvironment (TME) of typical types such as triple‐negative breast cancer is featured by hypoxia and immunosuppression with abundant tumor‐associated macrophages (TAMs), which also emerge potential therapeutic targets for antitumor therapy. M1‐like macrophage‐derived exosomes (M1‐Exos) have emerged a promising candidate their tumor‐targeting macrophage‐polarization capabilities. However, the limited drug‐loading efficiency stability M1‐Exos hindered effectiveness in applications. Here, hybrid nanovesicle developed integrating AS1411 aptamer‐conjugated liposomes (AApt‐Lips), termed M1E/AALs. obtained M1E/AALs are loaded perfluorotributylamine (PFTBA) IR780, P‐I, to construct P‐I@M1E/AALs reprogramming TME alleviating engineering TAMs. P‐I@M1E/AAL‐mediated therapy enhances situ generation reactive oxygen species, repolarizes TAMs toward an phenotype, promotes infiltration T lymphocytes. synergistic based on significantly suppresses growth prolongs survival 4T1‐tumor‐bearing mice. By multiple treatment modalities, P‐I@M1E/AAL nanoplatform demonstrates approach overcoming hypoxic immunosuppressive targeted TAM enhanced photodynamic immunotherapy. This study highlights innovative TAM‐engineering platform tumors characterized TME.

Language: Английский

Citations

23
Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy DOI Creative Commons

Ming‐Hui Fan,

Jin-Kui Pi,

Chen‐Yu Zou

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

Citations

22
Cell Membrane-derived Nanovesicles as Extracellular Vesicle-mimetics in Wound Healing DOI Creative Commons
Wen Li, Huihui Zhang, Lianglong Chen

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101595 - 101595

Published: Feb. 19, 2025

Language: Английский

Citations

4
Advancement of Nanomaterials- and Biomaterials-Based Technologies for Wound Healing and Tissue Regenerative Applications DOI
Durba Banerjee,

Kalyan Vydiam,

Venugopal Vangala

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Patients and healthcare systems face significant social financial challenges due to the increasing number of individuals with chronic external internal wounds that fail heal. The complexity healing process remains a serious health concern, despite effectiveness conventional wound dressings in promoting healing. Recent advancements materials science fabrication techniques have led development innovative enhance To further expedite process, novel approaches such as nanoparticles, 3D-printed dressings, biomolecule-infused emerged, along cell-based methods. Additionally, gene therapy technologies are being harnessed generate stem cell derivatives more functional, selective, responsive than their natural counterparts. This review highlights potential biomaterials, 3D bioprinting, gene- therapies However, it also underscores necessity for research address existing integrate these strategies into standard clinical practice.

Language: Английский

Citations

3
Extracellular Matrix-Mimicking Hydrogel with Angiogenic and Immunomodulatory Properties Accelerates Healing of Diabetic Wounds by Promoting Autophagy DOI
Yujie Pan, Yangyang Li, Xin Zhou

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

The management of diabetic wounds faces significant challenges due to the excessive activation reactive oxygen species (ROS), dysregulation inflammatory response, and impaired angiogenesis. A substantial body evidence suggests that aforementioned diverse factors contributing delayed healing may be associated with autophagy. Impaired autophagy leads endothelial fibroblast dysfunction impedes macrophage phenotypic transformation. This disruption hinders angiogenesis extracellular matrix deposition, ultimately culminating in wound healing. Therefore, biomaterials possessing regulatory functions hold potential for clinical applications enhancing wounds. hybrid multifunctional hydrogel (GelMa@SIS-Qu) has been developed, comprising methacrylamide gelatin (GelMa), a small intestine submucosal acellular (SIS), quercetin nanoparticles, which demonstrates capability promote promotion not only reduces ROS levels cells enhances their antioxidant activity but also mitigates ROS-induced cell apoptosis, thereby promoting Furthermore, facilitates transformation macrophages from M1 phenotype M2 phenotype. study investigates distinctive mechanisms GelMa@SIS-Qu proposes promising therapeutic strategy treating diabetes-related

Language: Английский

Citations

2
Multifunctional hydrogel-based engineered extracellular vesicles delivery for complicated wound healing DOI Creative Commons
Zuhao Li,

Jinlong Liu,

Jian Song

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4198 - 4217

Published: Jan. 1, 2024

The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.

Language: Английский

Citations

15