Recapitulating essential pathophysiological characteristics in lung-on-a-chip for disease studies DOI Creative Commons
Yanning Zhang, Xuejiao Wang, Yaoqing Yang

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 28, 2023

Lung diseases have become a significant challenge to public healthcare worldwide, which stresses the necessity of developing effective biological models for pathophysiological and pharmacological studies human respiratory system. In recent years, lung-on-a-chip has been extensively developed as potentially revolutionary model paradigm with high efficiency improved accuracy, bridging gap between cell culture preclinical trials. The advantages technology derive from its capabilities in establishing 3D multicellular architectures dynamic microphysiological environments. A critical issue development is utilizing such recapitulate essential components system effectively restoring physiological functions illustrating disease progress. Here we present review technology, highlighting various strategies capturing lung pathological characteristics. key characteristics lungs are examined, including airways, alveoli, alveolar septum. Accordingly, research capture analyzed. Recent pneumonia, cancer, asthma, chronic obstructive pulmonary disease, fibrosis based on surveyed. Finally, cross-disciplinary approaches proposed foster future technology.

Language: Английский

Tunable metacrylated silk fibroin-based hybrid bioinks for the bioprinting of tissue engineering scaffolds DOI
Jin Yang, Zhihui Li, Shikai Li

et al.

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(5), P. 1895 - 1909

Published: Jan. 1, 2023

Three-dimensional (3D) bioprinting is a powerful technique for the production of tissue-like structures to study cell behavior and tissue properties. A major challenge in 3D extrusion limited diversity bioinks, which fulfills requirements shear-thinning strain recovery behaviors can be solidified by crosslinking process retain their shape after printing. Herein, we aimed develop natural biopolymer-based formula with dual performance formulate cell-laden bioink. In this study, methacrylate gelatin (GelMA) methacrylated silk fibroin (SFMA) different degrees methacrylation were fabricated into hybrid bioinks. The GelMA/SFMA bioink an optimal degree provides excellent rheological properties bioprinting, its hydrogel precursor polymer form network at low temperature high fidelity printed construct through photocrosslinking. Moreover, encapsulate types cells together create constructs that mimic cellular microenvironment microscale level. Human umbilical vein endothelial (HUVECs) rat pheochromocytoma (PC12) encapsulated maintain viability proliferation ability long time. Furthermore, hydrogels implanted subcutaneous SD rats evaluation biocompatibility degradability vivo. Thus, proposed expands palette available bioinks offers opportunities biomedical applications such as engineering soft robotics clinical applications.

Language: Английский

Citations

19
3D Printing Approaches to Engineer Cardiac Tissue DOI Creative Commons
Tingyu Lu, Yi Xiang, Min Tang

et al.

Current Cardiology Reports, Journal Year: 2023, Volume and Issue: 25(6), P. 505 - 514

Published: May 2, 2023

Bioengineering of functional cardiac tissue composed primary cardiomyocytes has great potential for myocardial regeneration and in vitro modeling. 3D bioprinting was developed to create hydrogels that can mimic the structural, physiological, features native myocardium. Through a detailed review printing technologies bioink materials used creation heart tissue, this article discusses engineered tissues biomedical applications.

Language: Английский

Citations

18
Advances in Biomedical Applications of Solution Blow Spinning DOI Open Access

Javier Carriles,

Paul Nguewa, Gustavo González‐Gaitano

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14757 - 14757

Published: Sept. 29, 2023

In recent years, Solution Blow Spinning (SBS) has emerged as a new technology for the production of polymeric, nanocomposite, and ceramic materials in form nano microfibers, with similar features to those achieved by other procedures. The advantages SBS over spinning methods are fast generation fibers simplicity experimental setup that opens up possibility their on-site production. While producing large number nanofibers short time is crucial factor large-scale manufacturing, situ generation, example, sprayable, multifunctional dressings, capable releasing embedded active agents on wounded tissue, or use operating rooms prevent hemostasis during surgical interventions, open wide range possibilities. interest this evident from growing patents issued articles published last few years. Our focus review biomedicine-oriented applications based collection most relevant scientific papers date. Drug delivery, 3D culturing, regenerative medicine, fabrication biosensors some areas which been explored, frequently at proof-of-concept level. promising results obtained demonstrate potential biomedical pharmaceutical fields.

Language: Английский

Citations

17
Advances and challenges in organ-on-chip technology: toward mimicking human physiology and disease in vitro DOI

Dhiraj Kumar,

Rahul Nadda, Ramjee Repaka

et al.

Medical & Biological Engineering & Computing, Journal Year: 2024, Volume and Issue: 62(7), P. 1925 - 1957

Published: March 4, 2024

Language: Английский

Citations

8
Bioprinting of Perfusable Vascularized Organ Models for Drug Development via Sacrificial‐Free Direct Ink Writing DOI Creative Commons
Dongwei Wu, Shumin Pang, Johanna Berg

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(30)

Published: March 29, 2024

Abstract 3D bioprinting enables the fabrication of human organ models that can be used for various fields biomedical research, including oncology and infection biology. An important challenge, however, remains generation vascularized, perfusable closely simulate natural physiology. Here, a novel direct ink writing (DIW) approach is described produce vascularized without using sacrificial materials during fabrication. The high resolution method allows one‐step sophisticated hollow geometries. This sacrificial‐free DIW (SF‐DIW) to fabricate hepatic metastasis cancer types different formats investigating cytostatic activity anti‐cancer drugs. To this end, are incorporated into newly developed perfusion system with integrated micropumps an agar casting step improves physiological features bioprinted tissues. It shown environment tumor capable activating prodrug, which inhibits breast growth. versatile SF‐DIW able complicated constructs or microfluidic chips in straightforward cost‐efficient manner. also easily adapted other cell generating tissues may support development new therapeutics.

Language: Английский

Citations

8
Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma DOI Creative Commons
Ilkay Irem Ozbek, Hale Saybaşılı, Kutlu Ö. Ülgen

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(5), P. 2616 - 2635

Published: April 26, 2024

Primary brain tumor is one of the most fatal diseases. The malignant type among them, glioblastoma (GBM), has low survival rates. Standard treatments reduce life quality patients due to serious side effects. Tumor aggressiveness and unique structure render removal tumors development new therapies challenging. To elucidate characteristics examine their response drugs, realistic systems that mimic environment cellular crosstalk are desperately needed. In past decade, 3D GBM models have been presented as excellent platforms they allowed investigation phenotypes testing innovative therapeutic strategies. scope, bioprinting technology offers utilities such fabricating bioprinted structures in a layer-by-layer manner precisely controlled deposition materials cells, can be integrated with other technologies like microfluidics approach. This Review covers studies investigated models, especially using techniques essential parameters affect result study frequently used physical hydrogel, conditions, cross-linking methods, characterization techniques.

Language: Английский

Citations

8
Heart-on-a-chip systems: disease modeling and drug screening applications DOI
Derrick Butler, Darwin R. Reyes

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1494 - 1528

Published: Jan. 1, 2024

Heart-on-chip systems are one of the tools at forefront in race to develop alternative vitro preclinical testing for disease modeling, drug toxicity and efficacy assessment. Further innovation standardization will accelerate their use.

Language: Английский

Citations

7
Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation DOI

Ana Paula Pereira Guimaraes,

Italo Rodrigo Calori, Roberta Sessa Stilhano

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025024 - 025024

Published: Feb. 26, 2024

'On-a-chip' technology advances the development of physiologically relevant organ-mimicking architecture by integrating human cells into three-dimensional microfluidic devices. This method also establishes discrete functional units, faciliting focused research on specific organ components. In this study, we detail and assessment a convoluted renal proximal tubule-on-a-chip (PT-on-a-chip). platform involves co-culturing Renal Proximal Tubule Epithelial Cells (RPTEC) Human Umbilical Vein Endothelial (HUVEC) within polydimethylsiloxane device, crafted through combination 3D printing molding techniques. Our PT-on-a-chip significantly reduced high glucose level, exhibited albumin uptake, simulated tubulopathy induced amphotericin B. Remarkably, RPTEC:HUVEC co-culture efficient cell adhesion 30 min microchannels functionalized with plasma, 3-aminopropyltriethoxysilane, type-I collagen. approach required incubation time for medium perfusion. comparison, alternative methods such as plasma plus polyvinyl alcohol were only effective in promoting attachment to flat surfaces. The holds great promise valuable tool assessing nephrotoxic potential new drug candidates, enhancing our understanding interactions co-cultured cells, reducing need animal experimentation, safe ethical pharmaceuticals.

Language: Английский

Citations

7
Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine DOI
Dilip Kumar Chandra, Rui L. Reis, Subhas C. Kundu

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(7), P. 4145 - 4174

Published: June 1, 2024

3D bioprinting is recognized as the ultimate additive biomanufacturing technology in tissue engineering and regeneration, augmented with intelligent bioinks bioprinters to construct tissues or organs, thereby eliminating stipulation for artificial organs. For of soft tissues, such kidneys, hearts, other human body parts, formulations bioink enhanced bioinspired rheological mechanical properties were essential. Nanomaterials-based hybrid have potential overcome above-mentioned problem require much attention among researchers. Natural synthetic nanomaterials carbon nanotubes, graphene oxides, titanium nanosilicates, nanoclay, nanocellulose, etc. their blended been used various benefitted bioprintability, biocompatibility, biodegradability. A limited number articles published, requirement pushed us write this review. We reviewed, explored, discussed nanocomposite-based technology, properties, natural, synthetic, nanomaterial-based bioinks, including applications challenges, limitations, ethical considerations, solution future perspective, technological advancement efficient cost-effective methods regeneration healthcare.

Language: Английский

Citations

7
Machine learning‐enabled optimization of melt electro‐writing three‐dimensional printing DOI Creative Commons
Ahmed Abdullah,

Olgac Özarslan,

Sara Soltanabadi Farshi

et al.

Aggregate, Journal Year: 2024, Volume and Issue: 5(3)

Published: Jan. 4, 2024

Abstract Melt electrowriting (MEW) is a solvent‐free (i.e., no volatile chemicals), high‐resolution three‐dimensional (3D) printing method that enables the fabrication of semi‐flexible structures with rigid polymers. Despite its advantages, MEW process sensitive to changes in parameters (e.g., voltage, pressure, and temperature), which can cause fluid column breakage, jet lag, and/or fiber pulsing, ultimately deteriorating resolution quality. In spite commonly used error‐and‐trial determine most suitable parameters, here, we present machine learning (ML)‐enabled image analysis‐based for determining optimum through an easy‐to‐use graphical user interface (GUI). We trained five different ML algorithms using 168 3D print samples, among Gaussian regression model yielded 93% accuracy variability dependent variable, 0.12329 on root mean square error validation set 0.015201 predicting line thickness. Integration control feedback loop reduce steps prior process, decreasing time increasing overall throughput MEW) material waste improving cost‐effectiveness MEW). Moreover, embedding system GUI facilitates more straightforward use ML‐based optimization techniques industrial section users skills).

Language: Английский

Citations

6