NIR‐II AIE Luminogen‐Based Erythrocyte‐Like Nanoparticles with Granuloma‐Targeting and Self‐Oxygenation Characteristics for Combined Phototherapy of Tuberculosis

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(38)

Published: July 28, 2024

Abstract Tuberculosis, a fatal infectious disease caused by Mycobacterium tuberculosis ( M.tb ), is difficult to treat with antibiotics due drug resistance and short half‐life. Phototherapy represents promising alternative in combating . Exploring an intelligent material allowing effective treatment definitely appealing, yet significantly challenging task. Herein, all‐in‐one biomimetic therapeutic nanoparticle featured aggregation‐induced second near‐infrared emission, granuloma‐targeting, self‐oxygenation constructed, which can serve for prominent fluorescence imaging‐navigated combined phototherapy toward tuberculosis. After camouflaging the erythrocyte membrane, nanoparticles show prolonged blood circulation increased selective accumulation granuloma. Upon laser irradiation, loading photosensitizer of emission elevates production reactive oxygen species (ROS), causing damage death. The delivery relieve hypoxic granuloma microenvironment supports ROS generation during photodynamic therapy. Meanwhile, photothermal agent, Prussian blue nanoparticles, plays role good killing effect on Moreover, growth proliferation colonies are effectively inhibited nanoparticle‐treated tuberculous model mice, suggesting effects enhancing therapy

Language: Английский

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Functional probes for the diagnosis and treatment of infectious diseases

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Infectious diseases present significant challenges to global health, thereby extensively affecting both human society and the economy. In recent years, functional probes have demonstrated remarkable potential as crucial biomedical media for research treatment of infectious diseases. Their applications in realm include pathogen detection, exploration biological mechanisms, development anti‐infective drugs. This review provides a concise introduction severity, classification, pathogenesis Subsequently, we examined distinctiveness design strategies diagnosing treating diseases, shedding light on their rationale using typical examples. We discuss current status associated with clinical implementation probes. Furthermore, explored prospects these diagnosis aims offer novel insights into diagnostic broaden disease treatment.

Language: Английский

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Mechanics‐Resilient HA/SIS‐Based Composite Scaffolds with ROS‐Scavenging and Bacteria‐Resistant Capacity to Address Infected Bone Regeneration

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(24)

Published: Jan. 23, 2024

Abstract To address and regenerate infected bone defects complicated by issues such as inflammation resorption, to promote regeneration, this study focuses on the development of a composite scaffold with reactive oxygen species (ROS)‐scavenging bacteria‐resistant properties. The integrates self‐assembled small intestinal submucosa (SIS) hydrogel pre‐adsorbed hydroxyapatite (HA) particles tannic acid (TA), demonstrating distinctive mechanical resilience porous structures, suitable for filling irregular cavities facilitating cell infiltration, while exhibiting broad‐spectrum antibacterial efficacy robust ROS‐scavenging capacity tissue regeneration. RNA‐sequencing analysis indicates underlying mechanism revealing disrupting arginine alanine amino biosynthesis. Furthermore, demonstrates excellent cytocompatibility, viability exceeding 70%. Remarkably, it exceptional anti‐inflammatory performances (≈5‐fold control). In an defect model, facilitates superior being ≈5‐fold greater than control, maintaining conducive environment adhesion infiltration without collapse. This multifunctional emerges promising candidate combating infections in showcasing its potential addressing complex bone‐related challenges.

Language: Английский

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Stem cells vesicles-loaded type I pro-photosensitizer for synergetic oxygen-independent phototheranostics and microenvironment regulation in infected diabetic wounds

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159239 - 159239

Published: Jan. 10, 2025

Language: Английский

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Comprehensive Analysis of blaCTX-M1 Gene Expression Alongside iutA, csgA, and kpsMII Virulence Genes in Septicemic Escherichia coli Using Real-Time PCR

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 95 - 95

Published: Jan. 6, 2025

Background: Sepsis is a serious worldwide health concern, and Escherichia coli (E. coli) the main cause. This study investigates co-expression of blaCTX-M1 iutA, csgA, kpsMII genes in E. isolated from septicemic patients, aiming to clarify interaction between virulence resistance. Methods: evaluated 100 isolates patients. With disc diffusion method, antibiotic susceptibility was confirmed. The use ceftazidime-clavulanic acid allowed for confirmation ESBL. PCR real-time were used detect beta-lactamase genes. expression levels important compared LB blood. Results: Antibiotic resistance common carrying blaCTX-M1, including tetracycline (93%) erythromycin (99%). Instead, there no fosfomycin 3% carbapenems. Real-time revealed more blood csgA. Pathogenicity increased with csgA Conclusions: coexistence ESBL significantly increases infection severity. Monitoring these critical developing effective therapeutic strategies. key treating diseases having sophisticated diagnostic tools using antibiotics cautiously.

Language: Английский

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Evaluation of the probiotic, anti-microbial, anti-biofilm, and safety properties of Levilactobacillus brevis Lb13H

LWT, Journal Year: 2024, Volume and Issue: 207, P. 116636 - 116636

Published: Aug. 15, 2024

In this research, the probiotic potential, anti-microbial, anti-biofilm, and safety properties of Levilactobacillus brevis Lb13H were investigated. Also, Gaussian Process Regression (GPR) model was applied to predict some experimental parameters. The strain demonstrated resistance acidic pH, gastric intestinal juices, bile salts. Additionally, exhibited 45.34% surface hydrophobicity, 36.55% auto-aggregation capacity, 26.30% co-aggregation, 10.20% adhesion Caco-2 cells, 42.57% cholesterol removal, 48.63% radical-scavenging properties. able inhibit Listeria monocytogenes cells by 31.30%, its competitive inhibition percentage 37.5%. Furthermore, replacement L. with measured at 22.10%. most significant antimicrobial effect observed against monocytogenes, while least noted Rhizopus stolonifer. Haemolytic activity, DNase production, biogenic amine production not in strain, which also found be sensitive antibiotics ciprofloxacin chloramphenicol. cell-free supernatant effectively inhibited degraded biofilm formation achieving effectiveness rates 40.80% 36.60% respectively. results modeling indicated that there is any difference between actual predicted data GPR can variables high accuracy.

Language: Английский

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Advancing Aggregation‐Induced Emission‐Derived Biomaterials in Viral, Tuberculosis, and Fungal Infectious Diseases

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

ABSTRACT Contagious diseases caused by different types of highly contagious pathogens, such as SARS‐CoV‐2, monkeypox virus, Mycobacterium tuberculosis , and human immunodeficiency could trigger global outbreaks bring a huge public health burden. Advanced diagnostic, therapeutic, preventive strategies are urgently needed to deal with the epidemic diseases. Aggregation‐induced emission (AIE) has emerged one promising candidates that exhibit tunable photophysical properties, high biocompatibility, exceptional photostability, distinguishing aggregation‐enhanced fluorescence. As result, they offer effective for diagnosis, treatment, prevention This review systematically outlined latest research progress AIE‐based biomaterials mechanisms in The versatility AIE molecules, well efficient fluorescence potential innovative combat these challenges. Thanks recent advances materials science better understanding aggregation‐induced luminogens (AIEgens), AIEgens have great provide solutions detection, By reviewing state‐of‐the‐art methods killing, agents highlighting technological developments, this outlook aims promote development new means control emerging, re‐emerging, major further activities critical area research.

Language: Английский

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NIR-Activated Hydrogel with Dual-Enhanced Antibiotic Effectiveness for Thorough Elimination of Antibiotic-Resistant Bacteria

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Antibiotic resistance has become a critical health crisis globally. Traditional strategies using antibiotics can lead to drug-resistance, while inorganic antimicrobial agents cause severe systemic toxicity. Here, we have developed dual-antibiotic hydrogel delivery system (PDA-Ag@Levo/CMCS), which achieve controlled release of clinical levofloxacin (Levo) and classic nanoscale antibiotic silver nanoparticles (AgNPs), effectively eliminating drug-resistant P. aeruginosa. Benefiting from the photothermal (PTT) effect polydopamine (PDA), local high temperature generated by PDA-Ag@Levo/CMCS quickly kill bacteria through continuous responsive dual-antibiotics restore sensitivity ineffective antibiotics. Moreover, AgNPs could significantly improve efficiency traditional disrupting bacterial membranes reducing their toxicity healthy tissues. A clever combination PTT drug-combination therapy eliminate biofilms bacteria. Mechanism studies shown that PDA-Ag@Levo might aeruginosa biofilm formation protein synthesis, inhibit mutation promoting expression related genes, such as rpoS, dinB, mutS. Collectively, synergistic this combined with provides creative strategy for in chronic infection wounds.

Language: Английский

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Hydrogel integrating CBEO-CINcocktail (Cinnamomum pauciflorum Nees bark oil enriched with cinnamaldehyde) loaded nanosponges exhibited potent antibacterial activity against carbapenem resistant bacterial pathogens - in vitro and in vivo evaluation

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120503 - 120503

Published: Jan. 21, 2025

Language: Английский

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Extracellular Vesicle‐Inspired Therapeutic Strategies for the COVID‐19

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(29)

Published: June 25, 2024

Emerging infectious diseases like coronavirus pneumonia (COVID-19) present significant challenges to global health, extensively affecting both human society and the economy. Extracellular vesicles (EVs) have demonstrated remarkable potential as crucial biomedical tools for COVID-19 diagnosis treatment. However, due limitations in performance titer of natural vesicles, their clinical use remains limited. Nonetheless, EV-inspired strategies are gaining increasing attention. Notably, biomimetic inspired by EVs, possess specific receptors that can act "Trojan horses," preventing virus from infecting host cells. Genetic engineering enhance these enabling them carry more receptors, significantly specificity absorbing novel coronavirus. Additionally, inherit numerous cytokine parent cells, allowing effectively mitigate "cytokine storm" adsorbing pro-inflammatory cytokines. Overall, this strategy offers new avenues treatment emerging diseases. Herein, review systematically summarizes current applications COVID-19. The status associated with implementation also discussed. goal is provide insights into design expand application combating

Language: Английский

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