Biocatalysis: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(1), P. 88 - 119

Published: June 18, 2020

Abstract Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes organic synthesis, especially make chiral compounds for pharmaceuticals well the flavors and fragrance industry, are most prominent examples. In addition, biocatalysts used on a large scale specialty even bulk chemicals. This review intends give illustrative examples this field with special focus scalable production using enzymes. It also discusses opportunities limitations enzymatic syntheses distinct provides outlook emerging enzyme classes.

Language: Английский

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Electrochemical strategies for C–H functionalization and C–N bond formation

Chemical Society Reviews, Journal Year: 2018, Volume and Issue: 47(15), P. 5786 - 5865

Published: Jan. 1, 2018

This review provides an overview of the use electrochemistry as appealing platform for expediting carbon–hydrogen functionalization and carbon–nitrogen bond formation.

Language: Английский

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Engineering new catalytic activities in enzymes

Nature Catalysis, Journal Year: 2020, Volume and Issue: 3(3), P. 203 - 213

Published: Jan. 20, 2020

Language: Английский

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Opportunities and challenges for combining chemo- and biocatalysis

Nature Catalysis, Journal Year: 2018, Volume and Issue: 1(1), P. 12 - 22

Published: Jan. 8, 2018

Language: Английский

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Biocatalysis

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 24, 2021

Language: Английский

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Biocatalytic Oxidation Reactions: A Chemist's Perspective

Angewandte Chemie International Edition, Journal Year: 2018, Volume and Issue: 57(30), P. 9238 - 9261

Published: March 24, 2018

Abstract Oxidation chemistry using enzymes is approaching maturity and practical applicability in organic synthesis. Oxidoreductases (enzymes catalysing redox reactions) enable chemists to perform highly selective efficient transformations ranging from simple alcohol oxidations stereoselective halogenations of non‐activated C−H bonds. For many these reactions, no “classical” chemical counterpart known. Hence oxidoreductases open up shorter synthesis routes based on a more direct access the target products. The generally very mild reaction conditions may also reduce environmental impact biocatalytic reactions compared classical counterparts. In this Review, we critically summarise most important recent developments field oxidation identify pressing bottlenecks as well promising solutions.

Language: Английский

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The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Angewandte Chemie International Edition, Journal Year: 2019, Volume and Issue: 59(32), P. 13204 - 13231

Published: July 3, 2019

Directed evolution of stereo-, regio-, and chemoselective enzymes constitutes a unique way to generate biocatalysts for synthetically interesting transformations in organic chemistry biotechnology. In order this protein engineering technique be efficient, fast, reliable, also relevance synthetic chemistry, methodology development was still is necessary. Following description early key contributions, review focuses on recent developments. It includes optimization molecular biological methods gene mutagenesis the design efficient strategies their application, resulting notable reduction screening effort (bottleneck directed evolution). When aiming laboratory selectivity activity, second-generation versions Combinatorial Active-Site Saturation Test (CAST) Iterative Mutagenesis (ISM), both involving saturation (SM) at sites lining binding pocket, have emerged as preferred approaches, aided by silico such machine learning. The recently proposed Focused Rational Site-specific (FRISM) fusion rational evolution. On-chip solid-phase chemical synthesis rapid library construction enhances quality notably eliminating undesired amino acid bias, future evolution?

Language: Английский

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Extending the application of biocatalysis to meet the challenges of drug development

Nature Reviews Chemistry, Journal Year: 2018, Volume and Issue: 2(12), P. 409 - 421

Published: Nov. 20, 2018

Language: Английский

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Power of Biocatalysis for Organic Synthesis

ACS Central Science, Journal Year: 2021, Volume and Issue: 7(1), P. 55 - 71

Published: Jan. 14, 2021

Biocatalysis, using defined enzymes for organic transformations, has become a common tool in synthesis, which is also frequently applied industry. The generally high activity and outstanding stereo-, regio-, chemoselectivity observed many biotransformations are the result of precise control reaction active site biocatalyst. This achieved by exact positioning reagents relative to each other fine-tuned 3D environment, specific activating interactions between protein, subtle movements catalyst. Enzyme engineering enables one adapt catalyst desired process. A well-filled biocatalytic toolbox ready be used various reactions. Providing nonnatural conditions evolving biocatalysts play with myriad options creating novel transformations thereby opening new, short pathways target molecules. Combining several pot perform reactions concurrently increases efficiency biocatalysis even further.

Language: Английский

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Catalytic Homogeneous Asymmetric Hydrogenation: Successes and Opportunities

Organometallics, Journal Year: 2018, Volume and Issue: 38(1), P. 47 - 65

Published: Dec. 20, 2018

This is an overview of successes in the realm catalytic homogeneous asymmetric hydrogenation substrates primarily interest synthesis pharmaceuticals order to identify important problems still unsolved. First, tables are provided that list successful reductions over 90% enantiomeric excess prochiral ketones alcohols, imines amines, and olefins saturated carbon centers. Noted metal (including "green" metals Mn, Fe, Co) or enzyme, class ligand, conditions medium, scale reduction, if 1 kg product, as well nature process, whether direct using H2 gas (DH), transfer (TH), with dynamic kinetic resolution (DKR). Tables representative pharmaceutical fine chemicals products for each substrate. With this overview, opportunities further research development become clearer.

Language: Английский

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