Design of an in vitro biocatalytic cascade for the manufacture of islatravir

Science, Journal Year: 2019, Volume and Issue: 366(6470), P. 1255 - 1259

Published: Dec. 6, 2019

Enzyme-catalyzed reactions have begun to transform pharmaceutical manufacturing, offering levels of selectivity and tunability that can dramatically improve chemical synthesis. Combining enzymatic into multistep biocatalytic cascades brings additional benefits. Cascades avoid the waste generated by purification intermediates. They also allow be linked together overcome an unfavorable equilibrium or accumulation unstable inhibitory We report in vitro cascade synthesis investigational HIV treatment islatravir. Five enzymes were engineered through directed evolution act on non-natural substrates. These combined with four auxiliary construct islatravir from simple building blocks a three-step cascade. The overall requires fewer than half number steps previously reported routes.

Language: Английский

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Biocatalysis

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 24, 2021

Language: Английский

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Borrowing Hydrogen for Organic Synthesis

ACS Central Science, Journal Year: 2021, Volume and Issue: 7(4), P. 570 - 585

Published: March 25, 2021

Borrowing hydrogen is a process that used to diversify the synthetic utility of commodity alcohols. A catalyst first oxidizes an alcohol by removing form reactive carbonyl compound. This intermediate can undergo diverse range subsequent transformations before returns "borrowed" liberate product and regenerate catalyst. In this way, alcohols may be as alkylating agents whereby sole byproduct one-pot reaction water. recent decades, significant advances have been made in area, demonstrating many effective methods access valuable products. outlook highlights diversity metal biocatalysts are available for approach, well various performed, focusing on selection most advances. By succinctly describing conveying versatility borrowing chemistry, we anticipate its uptake will increase across wider scientific audience, expanding opportunities further development.

Language: Английский

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The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis

Chemical Science, Journal Year: 2020, Volume and Issue: 11(10), P. 2587 - 2605

Published: Jan. 1, 2020

Enzymes are excellent catalysts that increasingly being used in industry and academia. This Perspective provides a general practical guide to enzymes their synthetic potential, primarily aimed at organic chemists.

Language: Английский

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Photoenzymatic enantioselective intermolecular radical hydroalkylation

Nature, Journal Year: 2020, Volume and Issue: 584(7819), P. 69 - 74

Published: June 8, 2020

Language: Английский

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New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 50(10), P. 5850 - 5862

Published: Jan. 1, 2021

This tutorial review focuses on recent advances in technologies for enzyme immobilisation, enabling their cost-effective use the bio-based economy and continuous processing general.

Language: Английский

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Embracing Nature’s Catalysts: A Viewpoint on the Future of Biocatalysis

ACS Catalysis, Journal Year: 2020, Volume and Issue: 10(15), P. 8418 - 8427

Published: June 4, 2020

ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTEmbracing Nature's Catalysts: A Viewpoint on the Future of BiocatalysisBernhard Hauer*Bernhard HauerInstitute Biochemistry and Technical Biochemistry, Department Universitaet Stuttgart, Allmandring 31, 70569 Germany*Email: [email protected]More by Bernhard Hauerhttp://orcid.org/0000-0001-6259-3348Cite this: ACS Catal. 2020, 10, 15, 8418–8427Publication Date (Web):June 4, 2020Publication History Received16 April 2020Published online4 June inissue 7 August 2020https://pubs.acs.org/doi/10.1021/acscatal.0c01708https://doi.org/10.1021/acscatal.0c01708article-commentaryACS PublicationsCopyright © 2020 American Chemical Society. This publication is available under these Terms Use. Request reuse permissions free to access through this site. Learn MoreArticle Views15099Altmetric-Citations190LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score a quantitative measure attention that research has received online. Clicking donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (1 MB) Get e-AlertscloseSUBJECTS:Biocatalysis,Catalysts,Chemical reactions,Industrial manufacturing,Peptides proteins e-Alerts

Language: Английский

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The road to fully programmable protein catalysis

Nature, Journal Year: 2022, Volume and Issue: 606(7912), P. 49 - 58

Published: June 1, 2022

Language: Английский

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Advances in ultrahigh-throughput screening for directed enzyme evolution

Chemical Society Reviews, Journal Year: 2019, Volume and Issue: 49(1), P. 233 - 262

Published: Dec. 9, 2019

This review summarizes how ultrahigh-throughput screening methods employ cells and biomimetic compartments to access the vast, unexplored diversity of biocatalysts with novel functions derived from directed evolution metagenomics libraries.

Language: Английский

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RetroBioCat as a computer-aided synthesis planning tool for biocatalytic reactions and cascades

Nature Catalysis, Journal Year: 2021, Volume and Issue: 4(2), P. 98 - 104

Published: Jan. 4, 2021

Language: Английский

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