Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future DOI Creative Commons
Kamelia Boodhoo, Michael C. Flickinger, John M. Woodley

et al.

Chemical Engineering and Processing - Process Intensification, Journal Year: 2022, Volume and Issue: 172, P. 108793 - 108793

Published: Jan. 10, 2022

With the current pressing need to rise ambition of net zero targets mitigate carbon emissions and climate change impacts, sustainable processing has never been more critical. Bioprocessing all desirable attributes respond challenge: use cheap, renewable resources, nature-inspired, highly selective biocatalysts operating optimally under mild conditions reduced energy consumption/carbon footprint. bioprocessing productivity being far from ideal meet large-scale for food, drugs, biofuels bio-based chemicals, there tremendous interest late in developing intensified bioprocesses, with significant advancement achieved tailoring utilising technologies toolbox traditionally applied chemical process intensification. This review highlights wide range activities currently on-going bioprocess intensification, focusing on upstream, bioreactor/fermentation downstream separation steps. Great strides have made biocatalyst engineering high density cell immobilisation enhancement, which, conjunction elegant innovations such as novel bioreactor in-situ product separations, are enabling bioprocesses become competitive than ever before. The future prospects intensification promising but still challenges that be overcome fully exploit this technology.

Language: Английский

Biocatalysis: Enzymatic Synthesis for Industrial Applications DOI Creative Commons
Shuke Wu, Radka Šnajdrová, Jeffrey C. Moore

et al.

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(1), P. 88 - 119

Published: June 18, 2020

Abstract Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes organic synthesis, especially make chiral compounds for pharmaceuticals well the flavors and fragrance industry, are most prominent examples. In addition, biocatalysts used on a large scale specialty even bulk chemicals. This review intends give illustrative examples this field with special focus scalable production using enzymes. It also discusses opportunities limitations enzymatic syntheses distinct provides outlook emerging enzyme classes.

Language: Английский

Citations

1065
Water as the reaction medium in organic chemistry: from our worst enemy to our best friend DOI Creative Commons
Margery Cortes‐Clerget, Tzu‐Yu Yu, Joseph R. A. Kincaid

et al.

Chemical Science, Journal Year: 2021, Volume and Issue: 12(12), P. 4237 - 4266

Published: Jan. 1, 2021

A review presenting water as the logical reaction medium for future of organic chemistry. discussion is offered that covers both "on water" and "in phenomena, how playing unique roles in each, specifically with regard to its use synthesis.

Language: Английский

Citations

402
The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes DOI
Ge Qu, Aitao Li, Carlos G. Acevedo‐Rocha

et al.

Angewandte Chemie International Edition, Journal Year: 2019, Volume and Issue: 59(32), P. 13204 - 13231

Published: July 3, 2019

Directed evolution of stereo-, regio-, and chemoselective enzymes constitutes a unique way to generate biocatalysts for synthetically interesting transformations in organic chemistry biotechnology. In order this protein engineering technique be efficient, fast, reliable, also relevance synthetic chemistry, methodology development was still is necessary. Following description early key contributions, review focuses on recent developments. It includes optimization molecular biological methods gene mutagenesis the design efficient strategies their application, resulting notable reduction screening effort (bottleneck directed evolution). When aiming laboratory selectivity activity, second-generation versions Combinatorial Active-Site Saturation Test (CAST) Iterative Mutagenesis (ISM), both involving saturation (SM) at sites lining binding pocket, have emerged as preferred approaches, aided by silico such machine learning. The recently proposed Focused Rational Site-specific (FRISM) fusion rational evolution. On-chip solid-phase chemical synthesis rapid library construction enhances quality notably eliminating undesired amino acid bias, future evolution?

Language: Английский

Citations

399
Machine Learning in Enzyme Engineering DOI Creative Commons
Stanislav Mazurenko, Zbyněk Prokop, Jir̆ı́ Damborský

et al.

ACS Catalysis, Journal Year: 2019, Volume and Issue: 10(2), P. 1210 - 1223

Published: Dec. 13, 2019

Enzyme engineering plays a central role in developing efficient biocatalysts for biotechnology, biomedicine, and life sciences. Apart from classical rational design directed evolution approaches, machine learning methods have been increasingly applied to find patterns data that help predict protein structures, improve enzyme stability, solubility, function, substrate specificity, guide design. In this Perspective, we analyze the state of art databases used training validating predictors engineering. We discuss current limitations challenges which community is facing recent advancements experimental theoretical potential address those challenges. also present our view on possible future directions applications biocatalysts.

Language: Английский

Citations

342
Power of Biocatalysis for Organic Synthesis DOI Creative Commons
Christoph K. Winkler, Joerg H. Schrittwieser, Wolfgang Kroutil

et al.

ACS Central Science, Journal Year: 2021, Volume and Issue: 7(1), P. 55 - 71

Published: Jan. 14, 2021

Biocatalysis, using defined enzymes for organic transformations, has become a common tool in synthesis, which is also frequently applied industry. The generally high activity and outstanding stereo-, regio-, chemoselectivity observed many biotransformations are the result of precise control reaction active site biocatalyst. This achieved by exact positioning reagents relative to each other fine-tuned 3D environment, specific activating interactions between protein, subtle movements catalyst. Enzyme engineering enables one adapt catalyst desired process. A well-filled biocatalytic toolbox ready be used various reactions. Providing nonnatural conditions evolving biocatalysts play with myriad options creating novel transformations thereby opening new, short pathways target molecules. Combining several pot perform reactions concurrently increases efficiency biocatalysis even further.

Language: Английский

Citations

288
Embracing Nature’s Catalysts: A Viewpoint on the Future of Biocatalysis DOI Open Access
Bernhard Hauer

ACS Catalysis, Journal Year: 2020, Volume and Issue: 10(15), P. 8418 - 8427

Published: June 4, 2020

ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTEmbracing Nature's Catalysts: A Viewpoint on the Future of BiocatalysisBernhard Hauer*Bernhard HauerInstitute Biochemistry and Technical Biochemistry, Department Universitaet Stuttgart, Allmandring 31, 70569 Germany*Email: [email protected]More by Bernhard Hauerhttp://orcid.org/0000-0001-6259-3348Cite this: ACS Catal. 2020, 10, 15, 8418–8427Publication Date (Web):June 4, 2020Publication History Received16 April 2020Published online4 June inissue 7 August 2020https://pubs.acs.org/doi/10.1021/acscatal.0c01708https://doi.org/10.1021/acscatal.0c01708article-commentaryACS PublicationsCopyright © 2020 American Chemical Society. This publication is available under these Terms Use. Request reuse permissions free to access through this site. Learn MoreArticle Views15099Altmetric-Citations190LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score a quantitative measure attention that research has received online. Clicking donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (1 MB) Get e-AlertscloseSUBJECTS:Biocatalysis,Catalysts,Chemical reactions,Industrial manufacturing,Peptides proteins e-Alerts

Language: Английский

Citations

236
Synthetic biology 2020–2030: six commercially-available products that are changing our world DOI Creative Commons

Christopher A. Voigt

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Dec. 11, 2020

Synthetic biology will transform how we grow food, what eat, and where source materials medicines. Here I have selected six products that are now on the market, highlighting underlying technologies projecting forward to future can be expected over next ten years.

Language: Английский

Citations

227
Advances in ultrahigh-throughput screening for directed enzyme evolution DOI
Ulrich Markel, Khalil Essani,

Volkan Besirlioglu

et al.

Chemical Society Reviews, Journal Year: 2019, Volume and Issue: 49(1), P. 233 - 262

Published: Dec. 9, 2019

This review summarizes how ultrahigh-throughput screening methods employ cells and biomimetic compartments to access the vast, unexplored diversity of biocatalysts with novel functions derived from directed evolution metagenomics libraries.

Language: Английский

Citations

222
Two-Dimensional Polymers and Polymerizations DOI
Austin M. Evans, Michael J. Strauss, Amanda R. Corcos

et al.

Chemical Reviews, Journal Year: 2021, Volume and Issue: 122(1), P. 442 - 564

Published: Dec. 1, 2021

Synthetic chemists have developed robust methods to synthesize discrete molecules, linear and branched polymers, disordered cross-linked networks. However, two-dimensional polymers (2DPs) prepared from designed monomers been long missing these capabilities, both as objects of chemical synthesis in nature. Recently, new polymerization strategies characterization enabled the unambiguous realization covalently linked macromolecular sheets. Here we review 2DPs 2D methods. Three predominant emerged date, which produce either monolayers or multilayer assemblies. We discuss fundamental understanding scope each approaches, including: bond-forming reactions used, synthetic diversity prepared, their stacking behaviors, nanoscale mesoscale structures, macroscale morphologies. Additionally, describe analytical tools currently available characterize various isolated forms. Finally, emergent 2DP properties potential applications planar macromolecules. Throughout, highlight achievements identify opportunities for continued study.

Language: Английский

Citations

217
Fatty Acids and their Derivatives as Renewable Platform Molecules for the Chemical Industry DOI Creative Commons

Ursula Biermann,

Uwe T. Bornscheuer, Ivo Feußner

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(37), P. 20144 - 20165

Published: Feb. 22, 2021

Oils and fats of vegetable animal origin remain an important renewable feedstock for the chemical industry. Their industrial use has increased during last 10 years from 31 to 51 million tonnes annually. Remarkable achievements made in field oleochemistry this timeframe are summarized herein, including reduction fatty esters ethers, selective oxidation oxidative cleavage C-C double bonds, synthesis alkyl-branched compounds, isomerizing hydroformylation alkoxycarbonylation, olefin metathesis. The oleochemicals a great variety polymeric materials tremendously, too. In addition lipases phospholipases, other enzymes have found their way into biocatalytic oleochemistry. Important also generated new oil qualities existing crop plants or by using microorganisms optimized metabolic engineering.

Language: Английский

Citations

177