Structurally Dynamic Hydrogels for Biomedical Applications: Pursuing a Fine Balance between Macroscopic Stability and Microscopic Dynamics

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(18), P. 11149 - 11193

Published: June 30, 2021

Owing to their unique chemical and physical properties, hydrogels are attracting increasing attention in both basic translational biomedical studies. Although the classical with static networks have been widely reported for decades, a growing number of recent studies shown that structurally dynamic can better mimic dynamics functions natural extracellular matrix (ECM) soft tissues. These synthetic materials defined compositions recapitulate key biophysical properties living tissues, providing an important means understanding mechanisms by which cells sense remodel surrounding microenvironments. This review begins overall expectation design principles hydrogels. We then highlight progress fabrication strategies including degradation-dependent degradation-independent approaches, followed use applications such as regenerative medicine, drug delivery, 3D culture. Finally, challenges emerging trends development application discussed.

Language: Английский

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Direct imaging of single-molecule electrochemical reactions in solution

Nature, Journal Year: 2021, Volume and Issue: 596(7871), P. 244 - 249

Published: Aug. 11, 2021

Language: Английский

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Single Biomolecule Imaging by Electrochemiluminescence

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(43), P. 17910 - 17914

Published: Oct. 22, 2021

Herein, a single biomolecule is imaged by electrochemiluminescence (ECL) using Ru(bpy)32+-doped silica/Au nanoparticles (RuDSNs/AuNPs) as the ECL nanoemitters. The emission confined to local surface of RuDSNs leading significant enhancement in intensity. To prove concept, protein molecule at electrode initially visualized as-prepared RuDSN/AuNPs Furthermore, nanoemitter-labeled antibody linked cellular membrane image one cell, without interference current and optical background. success single-biomolecule imaging solves long-lasting task ultrasensitive analysis, which should be able provide more elegant information about biology.

Language: Английский

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Dual Intramolecular Electron Transfer for In Situ Coreactant‐Embedded Electrochemiluminescence Microimaging of Membrane Protein

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(1), P. 197 - 201

Published: Sept. 21, 2020

Abstract The demand for transporting coreactant to emitter and short lifetime of the radicals in electrochemiluminescence (ECL) emission inhibit greatly its application cytosensing microscopic imaging. Herein we designed a dual intramolecular electron transfer strategy tertiary amine conjugated polymer dots (TEA‐Pdots) develop coreactant‐embedded ECL mechanism microimaging system. TEA‐Pdots could produce at +1.2 V without need test solution. superstructure led unprecedented strength, which was 132 45 times stronger than those from mixture Pdots with TEA equivalent 62.5 higher amounts, respectively. efficiency even that typical [Ru(bpy) 3 ] 2+ Therefore, this system be used situ membrane protein on single living cells additional permeable treatment coreactant. feasibility validity were demonstrated by evaluating specific expression cell surface. This work opens new avenues applications analysis dynamic study biological events.

Language: Английский

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Rational Design of Electrochemiluminescent Devices

Accounts of Chemical Research, Journal Year: 2021, Volume and Issue: 54(14), P. 2936 - 2945

Published: June 24, 2021

ConspectusElectrochemiluminescence (ECL) is a light-emitting process which combines the intriguing merits of both electrochemical and chemiluminescent methods. It an extensively used method especially in clinical analysis biological research due to its high sensitivity, wide dynamic range, good reliability. ECL devices are critical for development applications ECL. Much effort has been expended improve portability, affordability, throughput new devices, allow adapt broad usage scenarios.In this Account, we summarize our efforts on recent including electrodes, based wireless power transfer (WPT) technique, novel bipolar electrochemistry. As essential components electrodes play important role detection. We have significantly improved sensitivity luminol detection H2O2 by using stainless steel electrode. By semiconductor materials (e.g., silicon BiVO4), exploited photoinduced generate intense emission at much lower potentials upon illumination. For convenience, disposability, cheap WPT designed. A small diode employed rectify alternating current into direct dramatically enhance intensity, enabling sensitive smart phone as detector. Finally, developed several electrochemistry view convenience multiplex sensing electrode (BPE). On basis feature BPE, movable BPEs BPE swimmers magnetic rotating BPE) deep exploration motional properties systems. To make full use solution, dispersed numerous micro-/nano-BPEs solution produce 3D entire instead 2D conventional devices. In addition, interference noise from driving was minimized introducing with passivation layer eliminate need fabrication arrays decrease applied voltage, develop new-type device consisting single-electrode system (SEES) resistance-induced potential difference. The SEES fabricated easily attaching multiperforated plate single film enables simultaneous many samples analytes only screen-printed electrode) arrays. great multiple-biomarker detection, drug screening, studies. Looking forward, believe that more related methods will be range applications, such vitro diagnosis, point-of-care testing, high-throughput analysis, study, mechanism investigation.

Language: Английский

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Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(21), P. 11769 - 11773

Published: March 12, 2021

Abstract Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of by electrochemiluminescence (ECL) microscopy. By regulating the concentrations luminophore and/or co‐reactant, thickness ECL layer can be controlled to match with spatial location different junctions. At a low concentration luminophore, generation is confined electrode surface, thus revealing only cell–matrix adhesions bottom cells. While high remarkably extended decreasing co‐reactant concentration, allowing sequential and cell–cell near apical surface cells, respectively. This strategy not provides new insights into mechanisms but also promises wide applications microscopy bioimaging.

Language: Английский

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Electrogenerated chemiluminescence detection of single entities

Chemical Science, Journal Year: 2021, Volume and Issue: 12(16), P. 5720 - 5736

Published: Jan. 1, 2021

Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL outstanding tool in analytical sensing. The traditional approach measures averaged electrochemical quantities large ensemble individual entities, including molecules, microstructures ions. However, real system usually heterogeneous, study single entities holds great potential elucidating new truths nature which are out assays or hidden complex systems. We would like review development intensity imaging based entity detection place emphasis on small micro/nanoparticles cells. current challenges for perspectives discussed.

Language: Английский

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The New Era of High-Throughput Nanoelectrochemistry

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(1), P. 319 - 356

Published: Jan. 10, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTThe New Era of High-Throughput NanoelectrochemistryXiangdong XuXiangdong XuDepartment Chemistry, University Warwick, Coventry CV4 7AL, U.K.More by Xiangdong XuView Biographyhttps://orcid.org/0000-0002-1120-4661, Dimitrios ValavanisDimitrios ValavanisDepartment ValavanisView Biographyhttps://orcid.org/0000-0002-8777-664X, Paolo CiocciPaolo CiocciUniversité Paris Cité, ITODYS, CNRS, F-75013 Paris, FranceMore CiocciView Biography, Samuel ConfederatSamuel ConfederatSchool Electronic and Electrical Engineering Pollard Institute, Leeds, Leeds LS2 9JT, U.K.Bragg Centre for Materials Research, ConfederatView Fabio MarcuccioFabio MarcuccioSchool U.K.Faculty Medicine, Imperial College London, London SW7 2AZ, United KingdomMore MarcuccioView Biographyhttps://orcid.org/0000-0003-4816-2896, Jean-François LemineurJean-François LemineurUniversité LemineurView Actis*Paolo ActisSchool U.K.*[email protected]More ActisView Biographyhttps://orcid.org/0000-0002-7146-1854, Frédéric Kanoufi*Frédéric KanoufiUniversité France*[email KanoufiView Biographyhttps://orcid.org/0000-0002-9784-2380, Patrick R. Unwin*Patrick UnwinDepartment UnwinView Biographyhttps://orcid.org/0000-0003-3106-2178Cite this: Anal. Chem. 2023, 95, 1, 319–356Publication Date (Web):January 10, 2023Publication History Received15 November 2022Published online10 January 2023Published inissue 10 2023https://doi.org/10.1021/acs.analchem.2c05105Copyright © 2023 The Authors. Published American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceCC: Creative CommonsBY: Credit must be given to the creatorArticle Views1582Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (5 MB) Get e-AlertsSUBJECTS:Ions,Nanoparticles,Nanopores,Probes,Surface charge e-Alerts

Language: Английский

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Single Cell Imaging of Electrochemiluminescence‐Driven Photodynamic Therapy

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(16)

Published: Feb. 15, 2022

Abstract We report a photodynamic therapy driven by electrochemiluminescence (ECL). The luminescence generated Ru(bpy) 3 2+ and co‐reactant tripropylamine (TPA) pair acts as both optical readout for ECL imaging, light source the excitation of photosensitizer to produce reactive oxygen species (ROS) in (PDT) system. ECL‐driven PDT (ECL‐PDT) relies on effective energy transfer from emission chlorin e6 (Ce6), which sensitizes surrounding O 2 into ROS. dynamic process gradual morphological changes, variation cell‐matrix adhesions, well increase cell membrane permeability ECL‐PDT were monitored under microscopy (ECLM) with good spatiotemporal resolution. Combining real‐time imaging ECL‐PDT, this new strategy provides not only insights cellular processes, but also promising potential clinical applications.

Language: Английский

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Nanoconfinement-Enhanced Electrochemiluminescence for in Situ Imaging of Single Biomolecules

ACS Nano, Journal Year: 2023, Volume and Issue: 17(4), P. 3809 - 3817

Published: Feb. 17, 2023

Direct imaging of electrochemical reactions at the single-molecule level is potential interest in materials, diagnostic, and catalysis applications. Electrochemiluminescence (ECL) offers opportunity to convert redox events into photons. However, it challenging capture single photons emitted from a ECL reaction specific location, thus limiting high-quality We developed nanoreactors based on Ru(bpy)32+-doped nanoporous zeolite nanoparticles (Ru@zeolite) for direct visualization nanoconfinement-enhanced reactions. Each nanoreactor not only acts as matrix host Ru(bpy)32+ molecules but also provides nanoconfined environment collision co-reactant radicals realize efficient situ The nanoscale confinement resulted enhanced ECL. Using such probes, dual-signal sensing protocol visual tracking biomolecule was performed. High-resolution membrane proteins heterogeneous cells effectively addressed with near-zero backgrounds. This could provide more sensitive tool individual biomolecules significantly advance biological

Language: Английский

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